Expression vector of S. pombe dg non-coding RNA gene SPNCRNA.232 genomic region containing dg intron.
Clone info. | Expression vector of S. pombe dg non-coding RNA gene SPNCRNA.232 consisting of shortened upstream sequence (299 bp), dg intron, and 106 bp downstream sequence with the nmt1 promoter and LEU2 marker. See Fig. 8 of Mutazono, M., PLoS Genet., 13 (2): e1006606, 2017. |
---|---|
Comment | Expression was confirmed by the depositor with RT-PCR |
Vector backbone | pREP1 (plasmid) |
Selectable markers | Amp^r (E. coli), LEU2 (S. pombe). |
Gene/insert name | Schizosaccharomyces pombe 972h- miscRNA (SPNCRNA.232) genomic |
Depositor|Developer | Tani, Tokio | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
---|---|
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mutazono, M. et al. PLoS Genet. 13(2): e1006606) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
---|---|
MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mutazono, M. et al. PLoS Genet. 13(2): e1006606)。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
---|---|---|---|---|
RDB15423 | pREP1-dg -3 | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pREP1-dg -3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15423). |
Reference section:
Mutazono, M., Morita, M., Tsukahara, C., Chinen, M., Nishioka, S., Yumikake, T., Dohke, K., Sakamoto, M., Ideue, T., Nakayama, J.I., Ishii, K., Tani, T., The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin. PLoS Genet. 13 (2): e1006606 (2017). PMID 28231281. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Mutazono, M., The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin. PLoS Genet. 13 (2): e1006606 (2017). PMID 28231281. [link to RRC of NBRP] |
---|
Featured content