Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAGIP-Gpnmb(YF)-FLAG/HA

Expression vector of mouse glycoprotein nmb (GPNMB) mutant (Y529F).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017).
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017)。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15414_A7J2p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 Sequence file: RDB15414_A7J2a.seq
>D04907A3_A7J2_1_pCAG-F_V2_F04_06_ABI08.ab1     1 GCTATCTTTG GTTCAAATCT TGTTGCGGAG CCGAATCTGG GAGGCGCCGC CGCACCCCCT    61 CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG CCGGCAGGAA GGAAATGGGC GGGGAGGGCC   121 TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCCTCTCCA GCCTCGGGGC TGTCCGCGGG   181 GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT CGGCTTCTGG CGTGTGACCG   241 GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG   301 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCAGA TCTGCCGCCA   361 TGGAAAGTCT CTGCGGGGTC CTGGGATTTC TGCTGCTGGC TGCAGGACTG CCTCTCCAGG   421 CTGCCAAGCG ATTTCGTGAT GTGCTGGGCC ATGAACAGTA TCCCGATCAC ATGAGAGAGC   481 ACAACCAATT ACGTGGCTGG TCTTCGGATG AAAATGAATG GGATGAACAC CTGTATCCAG   541 TGTGGAGGAG GGGAGACGGC AGGTGGAAGG ACTCCTGGGA AGGAGGCCGT GTGCAGGCAG   601 TCCTGACCAG TGACTCACCG GCTCTGGTGG GTTCCAATAT CACTTTTGTG GTGAACCTGG   661 TGTTCCCCAG ATGCCAGAAG GAAGATGCTA ATGGCAATAT CGTCTATGAG AAGAACTGCA   721 GGAATGATTT GGGACTGACA TCTGACCTGC ATGTCTACAA CTGGACTGCA GGGGCAGATG   781 ATGGTGACTG GGAAGATGGC ACCAGCCGAA GCCAGCATCT CAGGTTCCCG GACAGGAGGC   841 CCTTCCCTCG CCCCCATGGA TGGAAGAAAT GGAGCTTTGT CTACGTCTTT CACACACTTG   901 GCCAGTATTT CCAAAAACTG GGTCGGTGTT CAGCACGGGT TTCTATAAAC ACAGTCAACT   961 TGACAGCTGG CCCTCAGGTC ATGGAAAGTG ACTGTCTTTC GAGGATACCG GCCGGGCATA  1021 CATTCCCAAT CTCGAAGTGA AAGAATGTGT ATGTGAATAA CAGATCAGAT CCCTTGTTAT  1081 TCGTGGAACC AATGTCCCCA GAGAATTGAA CAAGGAAACT GTTCTTGATG AGATCCTCCT  1141 TCAGAAGAAC TTCCTCAATC GTCTTCCGAA TGTGGTCTCC TCTCCAATTC CTCAATGTAG //
Primer: IRES-R Sequence file: RDB15414_A7J2b.seq
>D04907A3_A7J2_1_IRES-R_G04_07_ABI08.ab1     1 GCAAAAGCGG GGCCTTCGGC CAGTAACGTT AGGGGGGGGG GAGGGAGAGG GGGGGGGGGC    61 GGAATTCCTC GAGCTAGAGG CTAGCATAAT CAGGAACATC ATACGGATAC TTGTCATCGT   121 CGTCCTTGTA GTCGCGGCCG CAGAGTGTCC TTGGCTTGTC CTGGAGCAAT GGATCCTTCT   181 CCTGGTCTCC TCGGAAGAAC GGGGCTTTCG CGTGACTGAG GAGAACACTC AGGCCCTTGC   241 CCTTGACCGT GTTCCTGGGG CAGTTTCCTA TTGGCTTGAA CGCCTTGTGT TTTTTGTACA   301 GCAAGATGGT AACCATGGTG ACAAGCACAG CCAGGCAGCC GATGGAGATC AGGACACCAT   361 TCACTGCTCT CAGAGGGGAG TCTGGGTCTT TGCCAGGGAT AGAGATCAGG GTGCTGGTGA   421 GGGCCAGGCT TGCATCATCT CCCAGAGTGA AATTCACACA GTAGGTGCCA GACCCATTGA   481 AGGCTCTTCT CACAGACAGC AGGCACAGCC CATCCACAGC CACAGGGCTG CAGACCCGGT   541 TCTGGGCGAT CTGGCAGGTG GGGTCGGAGA TGATCGTACA GGCTTCCATG GGGGTGGCCC   601 CTTTGCAGGT CACAGTGAAG TCCATCAGGG AGTTGGCAGG CTGCGGTGTG GGCATGGGGA   661 CATCTGCTAT CTGCATGATG CTGACTTCCA GGATCCCCTC TACAATTGTG ATGGTGGCTC   721 TGAAGTAGCC ATATCTGTTT ATTCGGCAGT TTTCATTGGA AATGTCACTC AGCTCCATGG   781 ATTTGTAACC AGTAGGCATT AAAGAGGGGC TAGGTGTTGA TAATGTGGGC AGAGGTGAGG   841 GCGGAGGTGA GGGCGGAGTT GAAGGTGGAG GCGGAGTCGA AGGCGAAGGG GGGGAGGGCA   901 TGGCCCCGGG CACTGCAGTT TGCACGGTGA GGTTAAGGTT GAAGGTTCCA TTGAGCACAT   961 AAGTGTGATT CAAAAGTGTG ATTGTTGGGA GACAAACAGG GCCAGTGTTG TTCCCCAAAA  1021 GTTCCACTTG TAGGAAAATG GCAAGAAGTT CGTTGAAGAA AGTGCTGGAT CAATGAATGG  1081 AGACCATCGA AGACGAATGG GAGGGTTCTC TGAGGAGGAT TCTCAATCCA GACAGGTTCC 1141 CTGGTCAATC TTCTGTGGGG ACAATG //
Primer: IRES-F Sequence file: RDB15414_A7J2c.seq
>D04907A3_A7J2_1_IRES-F_H04_08_ABI08.ab1     1 GCATGTCCTT TAAAATGGTT GTTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC    61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA   121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT   181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT   241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA   301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA   361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT   421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG   481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT   541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC   601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA   661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC   721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG   781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC   841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG   901 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTTC TGGGGGGGTG GGGGTGGGCA   961 GGACAGCAAG GGGGAGGATT GGAAGACAAT TAGCAGCCAT TGCTTGGGGA TGCGGTGGGC  1021 TTCTTATGGC TTCTGAAGGC GGAAAGAAAC CTTGCAAGCC TAAGCTTTGC GTAAATCATG  1081 GGTTCAATAG CTGTTTCCCT GGTGTGAATT TGTTTATCCG GCCTTCACAC AATTTCCCAC  1141 ACAAACTTAC GAAGGCTGGA AGCCTTATAA AGGTGTATAA AAGCCTCTGT GGGGGGGTGA 1201 CCTA //
Primer: IRES-F Sequence file: RDB15414_A7J2c.seq
>D04907A3_A7J2_1_IRES-F_H04_08_ABI08.ab1     1 GCATGTCCTT TAAAATGGTT GTTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC    61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA   121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT   181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT   241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA   301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA   361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT   421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG   481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT   541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC   601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA   661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC   721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG   781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC   841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG   901 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTTC TGGGGGGGTG GGGGTGGGCA   961 GGACAGCAAG GGGGAGGATT GGAAGACAAT TAGCAGCCAT TGCTTGGGGA TGCGGTGGGC  1021 TTCTTATGGC TTCTGAAGGC GGAAAGAAAC CTTGCAAGCC TAAGCTTTGC GTAAATCATG  1081 GGTTCAATAG CTGTTTCCCT GGTGTGAATT TGTTTATCCG GCCTTCACAC AATTTCCCAC  1141 ACAAACTTAC GAAGGCTGGA AGCCTTATAA AGGTGTATAA AAGCCTCTGT GGGGGGGTGA 1201 CCTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl