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pCAGIP-Gpnmb(YF)-FLAG/HA (#RDB15414)

Expression vector of mouse glycoprotein nmb (GPNMB) mutant (Y529F).

Clone info. Expression vector of mouse glycoprotein nmb (GPNMB) mutant (Y529F), tagged with FLAG and HA at C-terminal, SV40 ori, IRES-puromycin resistance, CAG promoter.
Comment Expression was confirmed by the depositor with immunoblotting and immunoprecipitation. Please refer Niwa, H. et al., Nat. Genet. 24 (4): 372-376, 2000 for pCAGIP vector.
Vector backbone pCAGIP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Mouse Gpnmb cDNA
Depositor|Developer Kato, Mitsuyasu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15414 pCAGIP-Gpnmb(YF)-FLAG/HA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGIP-Gpnmb(YF)-FLAG/HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15414).

Reference section:

Okita, Y., Kimura, M., Xie, R., Chen, C., Shen, L.T., Kojima, Y., Suzuki, H., Muratani, M., Saitoh, M., Semba, K., Heldin, C.H., Kato, M., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15414_A7J2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 ()
Sequence file: RDB15414_A7J2a.seq check
>D04907A3_A7J2_1_pCAG-F_V2_F04_06_ABI08.ab1
    1 GCTATCTTTG GTTCAAATCT TGTTGCGGAG CCGAATCTGG GAGGCGCCGC CGCACCCCCT
   61 CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG CCGGCAGGAA GGAAATGGGC GGGGAGGGCC
  121 TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCCTCTCCA GCCTCGGGGC TGTCCGCGGG
  181 GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT CGGCTTCTGG CGTGTGACCG
  241 GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
  301 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCAGA TCTGCCGCCA
  361 TGGAAAGTCT CTGCGGGGTC CTGGGATTTC TGCTGCTGGC TGCAGGACTG CCTCTCCAGG
  421 CTGCCAAGCG ATTTCGTGAT GTGCTGGGCC ATGAACAGTA TCCCGATCAC ATGAGAGAGC
  481 ACAACCAATT ACGTGGCTGG TCTTCGGATG AAAATGAATG GGATGAACAC CTGTATCCAG
  541 TGTGGAGGAG GGGAGACGGC AGGTGGAAGG ACTCCTGGGA AGGAGGCCGT GTGCAGGCAG
  601 TCCTGACCAG TGACTCACCG GCTCTGGTGG GTTCCAATAT CACTTTTGTG GTGAACCTGG
  661 TGTTCCCCAG ATGCCAGAAG GAAGATGCTA ATGGCAATAT CGTCTATGAG AAGAACTGCA
  721 GGAATGATTT GGGACTGACA TCTGACCTGC ATGTCTACAA CTGGACTGCA GGGGCAGATG
  781 ATGGTGACTG GGAAGATGGC ACCAGCCGAA GCCAGCATCT CAGGTTCCCG GACAGGAGGC
  841 CCTTCCCTCG CCCCCATGGA TGGAAGAAAT GGAGCTTTGT CTACGTCTTT CACACACTTG
  901 GCCAGTATTT CCAAAAACTG GGTCGGTGTT CAGCACGGGT TTCTATAAAC ACAGTCAACT
  961 TGACAGCTGG CCCTCAGGTC ATGGAAAGTG ACTGTCTTTC GAGGATACCG GCCGGGCATA
 1021 CATTCCCAAT CTCGAAGTGA AAGAATGTGT ATGTGAATAA CAGATCAGAT CCCTTGTTAT
 1081 TCGTGGAACC AATGTCCCCA GAGAATTGAA CAAGGAAACT GTTCTTGATG AGATCCTCCT
 1141 TCAGAAGAAC TTCCTCAATC GTCTTCCGAA TGTGGTCTCC TCTCCAATTC CTCAATGTAG
//
Primer: IRES-R ()
Sequence file: RDB15414_A7J2b.seq check
>D04907A3_A7J2_1_IRES-R_G04_07_ABI08.ab1
    1 GCAAAAGCGG GGCCTTCGGC CAGTAACGTT AGGGGGGGGG GAGGGAGAGG GGGGGGGGGC
   61 GGAATTCCTC GAGCTAGAGG CTAGCATAAT CAGGAACATC ATACGGATAC TTGTCATCGT
  121 CGTCCTTGTA GTCGCGGCCG CAGAGTGTCC TTGGCTTGTC CTGGAGCAAT GGATCCTTCT
  181 CCTGGTCTCC TCGGAAGAAC GGGGCTTTCG CGTGACTGAG GAGAACACTC AGGCCCTTGC
  241 CCTTGACCGT GTTCCTGGGG CAGTTTCCTA TTGGCTTGAA CGCCTTGTGT TTTTTGTACA
  301 GCAAGATGGT AACCATGGTG ACAAGCACAG CCAGGCAGCC GATGGAGATC AGGACACCAT
  361 TCACTGCTCT CAGAGGGGAG TCTGGGTCTT TGCCAGGGAT AGAGATCAGG GTGCTGGTGA
  421 GGGCCAGGCT TGCATCATCT CCCAGAGTGA AATTCACACA GTAGGTGCCA GACCCATTGA
  481 AGGCTCTTCT CACAGACAGC AGGCACAGCC CATCCACAGC CACAGGGCTG CAGACCCGGT
  541 TCTGGGCGAT CTGGCAGGTG GGGTCGGAGA TGATCGTACA GGCTTCCATG GGGGTGGCCC
  601 CTTTGCAGGT CACAGTGAAG TCCATCAGGG AGTTGGCAGG CTGCGGTGTG GGCATGGGGA
  661 CATCTGCTAT CTGCATGATG CTGACTTCCA GGATCCCCTC TACAATTGTG ATGGTGGCTC
  721 TGAAGTAGCC ATATCTGTTT ATTCGGCAGT TTTCATTGGA AATGTCACTC AGCTCCATGG
  781 ATTTGTAACC AGTAGGCATT AAAGAGGGGC TAGGTGTTGA TAATGTGGGC AGAGGTGAGG
  841 GCGGAGGTGA GGGCGGAGTT GAAGGTGGAG GCGGAGTCGA AGGCGAAGGG GGGGAGGGCA
  901 TGGCCCCGGG CACTGCAGTT TGCACGGTGA GGTTAAGGTT GAAGGTTCCA TTGAGCACAT
  961 AAGTGTGATT CAAAAGTGTG ATTGTTGGGA GACAAACAGG GCCAGTGTTG TTCCCCAAAA
 1021 GTTCCACTTG TAGGAAAATG GCAAGAAGTT CGTTGAAGAA AGTGCTGGAT CAATGAATGG
 1081 AGACCATCGA AGACGAATGG GAGGGTTCTC TGAGGAGGAT TCTCAATCCA GACAGGTTCC
1141 CTGGTCAATC TTCTGTGGGG ACAATG
//
Primer: IRES-F ()
Sequence file: RDB15414_A7J2c.seq check
>D04907A3_A7J2_1_IRES-F_H04_08_ABI08.ab1
    1 GCATGTCCTT TAAAATGGTT GTTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC
   61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA
  121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT
  181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT
  241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA
  301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA
  361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT
  421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG
  481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT
  541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC
  601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA
  661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC
  721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG
  781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
  841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG
  901 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTTC TGGGGGGGTG GGGGTGGGCA
  961 GGACAGCAAG GGGGAGGATT GGAAGACAAT TAGCAGCCAT TGCTTGGGGA TGCGGTGGGC
 1021 TTCTTATGGC TTCTGAAGGC GGAAAGAAAC CTTGCAAGCC TAAGCTTTGC GTAAATCATG
 1081 GGTTCAATAG CTGTTTCCCT GGTGTGAATT TGTTTATCCG GCCTTCACAC AATTTCCCAC
 1141 ACAAACTTAC GAAGGCTGGA AGCCTTATAA AGGTGTATAA AAGCCTCTGT GGGGGGGTGA
1201 CCTA
//
Primer: IRES-F ()
Sequence file: RDB15414_A7J2c.seq check
>D04907A3_A7J2_1_IRES-F_H04_08_ABI08.ab1
    1 GCATGTCCTT TAAAATGGTT GTTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC
   61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA
  121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT
  181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT
  241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA
  301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA
  361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT
  421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG
  481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT
  541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC
  601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA
  661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC
  721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG
  781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
  841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG
  901 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTTC TGGGGGGGTG GGGGTGGGCA
  961 GGACAGCAAG GGGGAGGATT GGAAGACAAT TAGCAGCCAT TGCTTGGGGA TGCGGTGGGC
 1021 TTCTTATGGC TTCTGAAGGC GGAAAGAAAC CTTGCAAGCC TAAGCTTTGC GTAAATCATG
 1081 GGTTCAATAG CTGTTTCCCT GGTGTGAATT TGTTTATCCG GCCTTCACAC AATTTCCCAC
 1141 ACAAACTTAC GAAGGCTGGA AGCCTTATAA AGGTGTATAA AAGCCTCTGT GGGGGGGTGA
1201 CCTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Okita, Y., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538. [link to RRC of NBRP]