Resource data sheet
Prev.

pCAGIP-Gpnmb(YF)-FLAG/HA

Expression vector of mouse glycoprotein nmb (GPNMB) mutant (Y529F).

Catalog number RDB15414
Resource name pCAGIP-Gpnmb(YF)-FLAG/HA
Clone info. Expression vector of mouse glycoprotein nmb (GPNMB) mutant (Y529F), tagged with FLAG and HA at C-terminal, SV40 ori, IRES-puromycin resistance, CAG promoter.
Expression was confirmed by the depositor with immunoblotting and immunoprecipitation. Please refer Niwa, H. et al., Nat. Genet. 24 (4): 372-376, 2000 for pCAGIP vector.
Vector backbone pCAGIP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Mouse Gpnmb cDNA
Depositor Kato, Mitsuyasu |
 
Other clones in our bank mouse Gpnmb (NCBI Gene 93695) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017).
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RDB15414 pCAGIP-Gpnmb(YF)-FLAG/HA DNA solution

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References and tips

Electronic file

Featured content

References

original Okita, Y., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15414_A7J2p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 RDB15414_A7J2a.seq
>D04907A3_A7J2_1_pCAG-F_V2_F04_06_ABI08.ab1
    1 GCTATCTTTG GTTCAAATCT TGTTGCGGAG CCGAATCTGG GAGGCGCCGC CGCACCCCCT
   61 CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG CCGGCAGGAA GGAAATGGGC GGGGAGGGCC
  121 TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCCTCTCCA GCCTCGGGGC TGTCCGCGGG
  181 GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT CGGCTTCTGG CGTGTGACCG
  241 GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
  301 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCAGA TCTGCCGCCA
  361 TGGAAAGTCT CTGCGGGGTC CTGGGATTTC TGCTGCTGGC TGCAGGACTG CCTCTCCAGG
  421 CTGCCAAGCG ATTTCGTGAT GTGCTGGGCC ATGAACAGTA TCCCGATCAC ATGAGAGAGC
  481 ACAACCAATT ACGTGGCTGG TCTTCGGATG AAAATGAATG GGATGAACAC CTGTATCCAG
  541 TGTGGAGGAG GGGAGACGGC AGGTGGAAGG ACTCCTGGGA AGGAGGCCGT GTGCAGGCAG
  601 TCCTGACCAG TGACTCACCG GCTCTGGTGG GTTCCAATAT CACTTTTGTG GTGAACCTGG
  661 TGTTCCCCAG ATGCCAGAAG GAAGATGCTA ATGGCAATAT CGTCTATGAG AAGAACTGCA
  721 GGAATGATTT GGGACTGACA TCTGACCTGC ATGTCTACAA CTGGACTGCA GGGGCAGATG
  781 ATGGTGACTG GGAAGATGGC ACCAGCCGAA GCCAGCATCT CAGGTTCCCG GACAGGAGGC
  841 CCTTCCCTCG CCCCCATGGA TGGAAGAAAT GGAGCTTTGT CTACGTCTTT CACACACTTG
  901 GCCAGTATTT CCAAAAACTG GGTCGGTGTT CAGCACGGGT TTCTATAAAC ACAGTCAACT
  961 TGACAGCTGG CCCTCAGGTC ATGGAAAGTG ACTGTCTTTC GAGGATACCG GCCGGGCATA
 1021 CATTCCCAAT CTCGAAGTGA AAGAATGTGT ATGTGAATAA CAGATCAGAT CCCTTGTTAT
 1081 TCGTGGAACC AATGTCCCCA GAGAATTGAA CAAGGAAACT GTTCTTGATG AGATCCTCCT
 1141 TCAGAAGAAC TTCCTCAATC GTCTTCCGAA TGTGGTCTCC TCTCCAATTC CTCAATGTAG
//
Primer: IRES-R RDB15414_A7J2b.seq
>D04907A3_A7J2_1_IRES-R_G04_07_ABI08.ab1
    1 GCAAAAGCGG GGCCTTCGGC CAGTAACGTT AGGGGGGGGG GAGGGAGAGG GGGGGGGGGC
   61 GGAATTCCTC GAGCTAGAGG CTAGCATAAT CAGGAACATC ATACGGATAC TTGTCATCGT
  121 CGTCCTTGTA GTCGCGGCCG CAGAGTGTCC TTGGCTTGTC CTGGAGCAAT GGATCCTTCT
  181 CCTGGTCTCC TCGGAAGAAC GGGGCTTTCG CGTGACTGAG GAGAACACTC AGGCCCTTGC
  241 CCTTGACCGT GTTCCTGGGG CAGTTTCCTA TTGGCTTGAA CGCCTTGTGT TTTTTGTACA
  301 GCAAGATGGT AACCATGGTG ACAAGCACAG CCAGGCAGCC GATGGAGATC AGGACACCAT
  361 TCACTGCTCT CAGAGGGGAG TCTGGGTCTT TGCCAGGGAT AGAGATCAGG GTGCTGGTGA
  421 GGGCCAGGCT TGCATCATCT CCCAGAGTGA AATTCACACA GTAGGTGCCA GACCCATTGA
  481 AGGCTCTTCT CACAGACAGC AGGCACAGCC CATCCACAGC CACAGGGCTG CAGACCCGGT
  541 TCTGGGCGAT CTGGCAGGTG GGGTCGGAGA TGATCGTACA GGCTTCCATG GGGGTGGCCC
  601 CTTTGCAGGT CACAGTGAAG TCCATCAGGG AGTTGGCAGG CTGCGGTGTG GGCATGGGGA
  661 CATCTGCTAT CTGCATGATG CTGACTTCCA GGATCCCCTC TACAATTGTG ATGGTGGCTC
  721 TGAAGTAGCC ATATCTGTTT ATTCGGCAGT TTTCATTGGA AATGTCACTC AGCTCCATGG
  781 ATTTGTAACC AGTAGGCATT AAAGAGGGGC TAGGTGTTGA TAATGTGGGC AGAGGTGAGG
  841 GCGGAGGTGA GGGCGGAGTT GAAGGTGGAG GCGGAGTCGA AGGCGAAGGG GGGGAGGGCA
  901 TGGCCCCGGG CACTGCAGTT TGCACGGTGA GGTTAAGGTT GAAGGTTCCA TTGAGCACAT
  961 AAGTGTGATT CAAAAGTGTG ATTGTTGGGA GACAAACAGG GCCAGTGTTG TTCCCCAAAA
 1021 GTTCCACTTG TAGGAAAATG GCAAGAAGTT CGTTGAAGAA AGTGCTGGAT CAATGAATGG
 1081 AGACCATCGA AGACGAATGG GAGGGTTCTC TGAGGAGGAT TCTCAATCCA GACAGGTTCC
1141 CTGGTCAATC TTCTGTGGGG ACAATG
//
Primer: IRES-F RDB15414_A7J2c.seq
>D04907A3_A7J2_1_IRES-F_H04_08_ABI08.ab1
    1 GCATGTCCTT TAAAATGGTT GTTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC
   61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA
  121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT
  181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT
  241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA
  301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA
  361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT
  421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG
  481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT
  541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC
  601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA
  661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC
  721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG
  781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
  841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG
  901 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTTC TGGGGGGGTG GGGGTGGGCA
  961 GGACAGCAAG GGGGAGGATT GGAAGACAAT TAGCAGCCAT TGCTTGGGGA TGCGGTGGGC
 1021 TTCTTATGGC TTCTGAAGGC GGAAAGAAAC CTTGCAAGCC TAAGCTTTGC GTAAATCATG
 1081 GGTTCAATAG CTGTTTCCCT GGTGTGAATT TGTTTATCCG GCCTTCACAC AATTTCCCAC
 1141 ACAAACTTAC GAAGGCTGGA AGCCTTATAA AGGTGTATAA AAGCCTCTGT GGGGGGGTGA
1201 CCTA
//
Primer: AmpR RDB15414_A7J2c.seq
>D04907A3_A7J2_1_IRES-F_H04_08_ABI08.ab1
    1 GCATGTCCTT TAAAATGGTT GTTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC
   61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA
  121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT
  181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT
  241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA
  301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA
  361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT
  421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG
  481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT
  541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC
  601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA
  661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC
  721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG
  781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
  841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG
  901 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTTC TGGGGGGGTG GGGGTGGGCA
  961 GGACAGCAAG GGGGAGGATT GGAAGACAAT TAGCAGCCAT TGCTTGGGGA TGCGGTGGGC
 1021 TTCTTATGGC TTCTGAAGGC GGAAAGAAAC CTTGCAAGCC TAAGCTTTGC GTAAATCATG
 1081 GGTTCAATAG CTGTTTCCCT GGTGTGAATT TGTTTATCCG GCCTTCACAC AATTTCCCAC
 1141 ACAAACTTAC GAAGGCTGGA AGCCTTATAA AGGTGTATAA AAGCCTCTGT GGGGGGGTGA
1201 CCTA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Okita, Y., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538.

2019.03.18

GNP_filter3_RDBDEP_html_190316.pl