Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAGIP-Gpnmb-FLAG/HA

Expression vector of mouse glycoprotein nmb (GPNMB).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017).
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017)。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15413_A7J2p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 Sequence file: RDB15413_A7J2a.seq
>D04907A2_A7J2_1_pCAG-F_V2_B04_02_ABI08.ab1     1 GAATACTTTG GTTCAAATCT GTGCGGAGCC GAATCTGGGA GGCGCCGCCG CACCCCCTCT    61 AGCGGGCGCG GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT   121 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CCTCTCCAGC CTCGGGGCTG TCCGCGGGGG   181 GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC   241 GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC   301 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCAGATC TGCCGCCATG   361 GAAAGTCTCT GCGGGGTCCT GGGATTTCTG CTGCTGGCTG CAGGACTGCC TCTCCAGGCT   421 GCCAAGCGAT TTCGTGATGT GCTGGGCCAT GAACAGTATC CCGATCACAT GAGAGAGCAC   481 AACCAATTAC GTGGCTGGTC TTCGGATGAA AATGAATGGG ATGAACACCT GTATCCAGTG   541 TGGAGGAGGG GAGACGGCAG GTGGAAGGAC TCCTGGGAAG GAGGCCGTGT GCAGGCAGTC   601 CTGACCAGTG ACTCACCGGC TCTGGTGGGT TCCAATATCA CTTTTGTGGT GAACCTGGTG   661 TTCCCCAGAT GCCAGAAGGA AGATGCTAAT GGCAATATCG TCTATGAGAA GAACTGCAGG   721 AATGATTTGG GACTGACATC TGACCTGCAT GTCTACAACT GGACTGCAGG GGCAGATGAT   781 GGTGACTGGG AAGATGGCAC CAGCCGAAGC CAGCATCTCA GGTTCCCGGA CAGGAGGCCC   841 TTCCCTCGCC CCCATGGATG GAAGAAAATG GAGCTTTGTC TACGTCTTTC ACACACTTGG   901 CCAGTATTTC CAAAAACTGG GTCGGTGTTC AGCACGGGGT TTCTATAACA CAGTCAACTT   961 GACAGCTGGC CCTCAAGGTC ATGGAAAGTG ACTGTCTTTT CGAAGATTAC GGCCCGGGGC  1021 ATTACATTTC CTCATCTCGG AAGGTGAAAA GATTGTGTAT TGGTGAATAA CAGATCAGGA  1081 TCCCTTGTAA TTCGGTGACC CATGTCCCAG AGGATTGAAC AGGAACTTGG TCTGAATGGA  1141 GATCCTTTCC CTTCGAAGAG AACCCTCCCT CATCGCGTCC CTTCGCGAAT TGTAGTGTCC 1201 TCA //
Primer: IRES-R Sequence file: RDB15413_A7J2b.seq
>D04907A2_A7J2_1_IRES-R_C04_03_ABI08.ab1     1 GCCACATACC GGGCCTTTCG GGCCAGTTAA CGTTAGGGGG GGGGGGAGGG AGAGGGGGGG    61 GGGGCGGAAT TCCTCGAGCT AGAGGCTAGC ATAATCAGGA ACATCATACG GATACTTGTC   121 ATCGTCGTCC TTGTAGTCGC GGCCGCAGAG TGTCCTTGGC TTGTCCTGGA GCAATGGATC   181 CTTCTCCTGG TCTCCTCGGA AGAACGGGGC TTTCGCGTGA CTGAGGAGAA CACTCAGGCC   241 CTTGCCCTTG ACCGTGTTCC TGGGGCAGTT TCCTATTGGC TTGTACGCCT TGTGTTTTTT   301 GTACAGCAAG ATGGTAACCA TGGTGACAAG CACAGCCAGG CAGCCAATGG AGATCAGGAC   361 ACCATTCACT GCTCTCAGAG GGGAGTCTGG GTCTTTGCCA GGGATAGAGA TCAGGGTGCT   421 GGTGAGGGCC AGGCTTGCAT CATCTCCCAG AGTGAAATTC ACACAGTAGG TGCCAGACCC   481 ATTGAAGGCT CTTCTCACAG ACAGCAGGCA CAGCCCATCC ACAGCCACAG GGCTGCAAAC   541 CCGGTTCTGG GCGATCTGGC AGGTGGGGTC GGAGATGATC GTACAGGCTT CCATGGGGGT   601 GGCCCCTTTG CAGGTCACAG TGAAGTCCAT CAGGGAGTTG GCAGGCTGCG GTGTGGGCAT   661 GGGGACATCT GCTATCTGCA TGATGCTGAC TTCCAGGATC CCCTCTACAA TTGTGATGGT   721 GGCTCTGAAG TAGCCAATAT CTGTTTATTC GGCAGTTTTC ATTGGAAAAT GTCACTCAGC   781 TCCATGGATT TGTAACCAGT AGGCATTAAA GAGGGGCTAG GGTGTTGATA ATGTGGGCAG   841 AGGTTGAGGG CGGAGGTGAG GGGCGGAGTT GAAGGTGGAG GCGGAGTCCG AAAGGCGAAG   901 GGGGAGGGGC ATGGCCCCGG GCACTGCAGT TTTGCACGGT GAGGTTAAAG GTTTGAAAGG   961 TTCCCATTTG AGCACCATAA GTTGTGATTT CAAAGTGGTG ATTTGTTTGT AAGACAAACA  1021 GGCCCAGTTG TTTGTTCCCC CAAAGGTTTC CACCTTTGTC AGGAAAAATG GCCGAAGTTC  1081 GTTTTGAGGA AAGTTGGCCT TGGGGATTCA TGTGAATATG AAGGGACATC TCGAAAGAAA  1141 ACGAATTGGG GGGAAGGTTC TTCTGTAAGG GAAAAATTCT TCATTCAGGA CCAAGATTTC 1201 TCCTCTGTGC TTCCCTATCT CTTCTCTCTG TGGA //
Primer: IRES-F Sequence file: RDB15413_A7J2c.seq
>D04907A2_A7J2_1_IRES-F_D04_04_ABI08.ab1     1 TACTAGCCTT TTTATGGTTG TTTTAGGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC    61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA   121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT   181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT   241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA   301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA   361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT   421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG   481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT   541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC   601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA   661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC   721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG   781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC   841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAATGAGGA   901 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGCAGGAC   961 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG  1021 GCTTCTGAGG CGGAAAGAAC CTTGCAGCCC AAGCTTGGCG TAAATCATGG GTCAATAAGC  1081 CTGTTTCCCT GTGTGAAAAT TTGATAATTC CGCCTTCCAC AATTCACACA CATACCGAGT 1141 CGGGAGGCAA TTAAAGTGTA AGCCCTTGGG GGTGCCCTTA ATG //
Primer: IRES-F Sequence file: RDB15413_A7J2c.seq
>D04907A2_A7J2_1_IRES-F_D04_04_ABI08.ab1     1 TACTAGCCTT TTTATGGTTG TTTTAGGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC    61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA   121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT   181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT   241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA   301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA   361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT   421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG   481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT   541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC   601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA   661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC   721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG   781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC   841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAATGAGGA   901 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGCAGGAC   961 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG  1021 GCTTCTGAGG CGGAAAGAAC CTTGCAGCCC AAGCTTGGCG TAAATCATGG GTCAATAAGC  1081 CTGTTTCCCT GTGTGAAAAT TTGATAATTC CGCCTTCCAC AATTCACACA CATACCGAGT 1141 CGGGAGGCAA TTAAAGTGTA AGCCCTTGGG GGTGCCCTTA ATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl