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pCAGIP-Gpnmb-FLAG/HA (#RDB15413)

Expression vector of mouse glycoprotein nmb (GPNMB).

Clone info. Expression vector of mouse glycoprotein nmb (GPNMB), tagged with FLAG and HA at C-terminal, SV40 ori, IRES-puromycin resistance, CAG promoter.
Comment Expression was confirmed by the depositor with immunoblotting and immunoprecipitation. Please refer Niwa, H. et al., Nat. Genet. 24 (4): 372-376, 2000 for pCAGIP vector.
Vector backbone pCAGIP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Mouse Gpnmb cDNA
Depositor|Developer Kato, Mitsuyasu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15413 pCAGIP-Gpnmb-FLAG/HA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGIP-Gpnmb-FLAG/HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15413).

Reference section:

Okita, Y., Kimura, M., Xie, R., Chen, C., Shen, L.T., Kojima, Y., Suzuki, H., Muratani, M., Saitoh, M., Semba, K., Heldin, C.H., Kato, M., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15413_A7J2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 ()
Sequence file: RDB15413_A7J2a.seq check
>D04907A2_A7J2_1_pCAG-F_V2_B04_02_ABI08.ab1
    1 GAATACTTTG GTTCAAATCT GTGCGGAGCC GAATCTGGGA GGCGCCGCCG CACCCCCTCT
   61 AGCGGGCGCG GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT
  121 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CCTCTCCAGC CTCGGGGCTG TCCGCGGGGG
  181 GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC
  241 GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC
  301 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCAGATC TGCCGCCATG
  361 GAAAGTCTCT GCGGGGTCCT GGGATTTCTG CTGCTGGCTG CAGGACTGCC TCTCCAGGCT
  421 GCCAAGCGAT TTCGTGATGT GCTGGGCCAT GAACAGTATC CCGATCACAT GAGAGAGCAC
  481 AACCAATTAC GTGGCTGGTC TTCGGATGAA AATGAATGGG ATGAACACCT GTATCCAGTG
  541 TGGAGGAGGG GAGACGGCAG GTGGAAGGAC TCCTGGGAAG GAGGCCGTGT GCAGGCAGTC
  601 CTGACCAGTG ACTCACCGGC TCTGGTGGGT TCCAATATCA CTTTTGTGGT GAACCTGGTG
  661 TTCCCCAGAT GCCAGAAGGA AGATGCTAAT GGCAATATCG TCTATGAGAA GAACTGCAGG
  721 AATGATTTGG GACTGACATC TGACCTGCAT GTCTACAACT GGACTGCAGG GGCAGATGAT
  781 GGTGACTGGG AAGATGGCAC CAGCCGAAGC CAGCATCTCA GGTTCCCGGA CAGGAGGCCC
  841 TTCCCTCGCC CCCATGGATG GAAGAAAATG GAGCTTTGTC TACGTCTTTC ACACACTTGG
  901 CCAGTATTTC CAAAAACTGG GTCGGTGTTC AGCACGGGGT TTCTATAACA CAGTCAACTT
  961 GACAGCTGGC CCTCAAGGTC ATGGAAAGTG ACTGTCTTTT CGAAGATTAC GGCCCGGGGC
 1021 ATTACATTTC CTCATCTCGG AAGGTGAAAA GATTGTGTAT TGGTGAATAA CAGATCAGGA
 1081 TCCCTTGTAA TTCGGTGACC CATGTCCCAG AGGATTGAAC AGGAACTTGG TCTGAATGGA
 1141 GATCCTTTCC CTTCGAAGAG AACCCTCCCT CATCGCGTCC CTTCGCGAAT TGTAGTGTCC
1201 TCA
//
Primer: IRES-R ()
Sequence file: RDB15413_A7J2b.seq check
>D04907A2_A7J2_1_IRES-R_C04_03_ABI08.ab1
    1 GCCACATACC GGGCCTTTCG GGCCAGTTAA CGTTAGGGGG GGGGGGAGGG AGAGGGGGGG
   61 GGGGCGGAAT TCCTCGAGCT AGAGGCTAGC ATAATCAGGA ACATCATACG GATACTTGTC
  121 ATCGTCGTCC TTGTAGTCGC GGCCGCAGAG TGTCCTTGGC TTGTCCTGGA GCAATGGATC
  181 CTTCTCCTGG TCTCCTCGGA AGAACGGGGC TTTCGCGTGA CTGAGGAGAA CACTCAGGCC
  241 CTTGCCCTTG ACCGTGTTCC TGGGGCAGTT TCCTATTGGC TTGTACGCCT TGTGTTTTTT
  301 GTACAGCAAG ATGGTAACCA TGGTGACAAG CACAGCCAGG CAGCCAATGG AGATCAGGAC
  361 ACCATTCACT GCTCTCAGAG GGGAGTCTGG GTCTTTGCCA GGGATAGAGA TCAGGGTGCT
  421 GGTGAGGGCC AGGCTTGCAT CATCTCCCAG AGTGAAATTC ACACAGTAGG TGCCAGACCC
  481 ATTGAAGGCT CTTCTCACAG ACAGCAGGCA CAGCCCATCC ACAGCCACAG GGCTGCAAAC
  541 CCGGTTCTGG GCGATCTGGC AGGTGGGGTC GGAGATGATC GTACAGGCTT CCATGGGGGT
  601 GGCCCCTTTG CAGGTCACAG TGAAGTCCAT CAGGGAGTTG GCAGGCTGCG GTGTGGGCAT
  661 GGGGACATCT GCTATCTGCA TGATGCTGAC TTCCAGGATC CCCTCTACAA TTGTGATGGT
  721 GGCTCTGAAG TAGCCAATAT CTGTTTATTC GGCAGTTTTC ATTGGAAAAT GTCACTCAGC
  781 TCCATGGATT TGTAACCAGT AGGCATTAAA GAGGGGCTAG GGTGTTGATA ATGTGGGCAG
  841 AGGTTGAGGG CGGAGGTGAG GGGCGGAGTT GAAGGTGGAG GCGGAGTCCG AAAGGCGAAG
  901 GGGGAGGGGC ATGGCCCCGG GCACTGCAGT TTTGCACGGT GAGGTTAAAG GTTTGAAAGG
  961 TTCCCATTTG AGCACCATAA GTTGTGATTT CAAAGTGGTG ATTTGTTTGT AAGACAAACA
 1021 GGCCCAGTTG TTTGTTCCCC CAAAGGTTTC CACCTTTGTC AGGAAAAATG GCCGAAGTTC
 1081 GTTTTGAGGA AAGTTGGCCT TGGGGATTCA TGTGAATATG AAGGGACATC TCGAAAGAAA
 1141 ACGAATTGGG GGGAAGGTTC TTCTGTAAGG GAAAAATTCT TCATTCAGGA CCAAGATTTC
1201 TCCTCTGTGC TTCCCTATCT CTTCTCTCTG TGGA
//
Primer: IRES-F ()
Sequence file: RDB15413_A7J2c.seq check
>D04907A2_A7J2_1_IRES-F_D04_04_ABI08.ab1
    1 TACTAGCCTT TTTATGGTTG TTTTAGGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC
   61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA
  121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT
  181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT
  241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA
  301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA
  361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT
  421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG
  481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT
  541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC
  601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA
  661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC
  721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG
  781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
  841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAATGAGGA
  901 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGCAGGAC
  961 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG
 1021 GCTTCTGAGG CGGAAAGAAC CTTGCAGCCC AAGCTTGGCG TAAATCATGG GTCAATAAGC
 1081 CTGTTTCCCT GTGTGAAAAT TTGATAATTC CGCCTTCCAC AATTCACACA CATACCGAGT
1141 CGGGAGGCAA TTAAAGTGTA AGCCCTTGGG GGTGCCCTTA ATG
//
Primer: IRES-F ()
Sequence file: RDB15413_A7J2c.seq check
>D04907A2_A7J2_1_IRES-F_D04_04_ABI08.ab1
    1 TACTAGCCTT TTTATGGTTG TTTTAGGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC
   61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA
  121 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT
  181 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT
  241 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA
  301 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA
  361 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT
  421 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG
  481 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT
  541 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC
  601 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA
  661 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC
  721 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG
  781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
  841 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAATGAGGA
  901 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGCAGGAC
  961 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG
 1021 GCTTCTGAGG CGGAAAGAAC CTTGCAGCCC AAGCTTGGCG TAAATCATGG GTCAATAAGC
 1081 CTGTTTCCCT GTGTGAAAAT TTGATAATTC CGCCTTCCAC AATTCACACA CATACCGAGT
1141 CGGGAGGCAA TTAAAGTGTA AGCCCTTGGG GGTGCCCTTA ATG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Okita, Y., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538. [link to RRC of NBRP]