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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGIP-FLAG-Mafk

Expression vector of mouse Mafk.

Catalog number RDB15412
Resource name pCAGIP-FLAG-Mafk
Clone info. Expression vector of mouse musculoaponeurotic fibrosarcoma (MAF) oncogene family protein K (Mafk), tagged with FLAG at N-terminal, SV40 ori, IRES-puromycin resistance, CAG promoter.
Comment Expression was confirmed by the depositor with immunoblotting. Please refer Niwa, H. et al., Nat. Genet. 24 (4): 372-376, 2000 for pCAGIP vector.
Vector backbone pCAGIP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Mouse Mafk cDNA
Depositor|Developer Kato, Mitsuyasu |
Other clones in our bank

External Database
Mouse Mafk

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017).
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017)。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15412 pCAGIP-FLAG-Mafk DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Okita, Y., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15412_A7J2p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V2
          Region: CAG pro,FLAG,insert5'
          Sequence file: RDB15412_A7J2a.seq check
          >D04907A1_A7J2_1_pCAG-F_V2_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NNNNTCTGGG NNGNGCCGCC GCACCCCCTC
             61 TAGCGGGCGC GGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT
            121 TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG CCTCGGGGCT GTCCGCGGGG
            181 GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC GGCTTCTGGC GTGTGACCGG
            241 CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG
            301 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCAGAT CCACCGCCAT
            361 GGACTACAAG GACGATGATG ACAAGGGCGA ATTCACGACT AATCCCAAGC CCAACAAGGC
            421 ATTGAAGGTC AAGAAGGAGG CGGGTGAGAA CGCCCCTGTT CTTAGCGATG ATGAGCTGGT
            481 GTCCATGTCA GTGCGGGAGC TAAACCAGCA CCTGCGGGGG CTCACCAAGG AGGAGGTCAC
            541 TCGGCTGAAG CAGCGGCGGC GCACACTCAA GAACAGAGGC TACGCGGCTA GCTGTCGCAT
            601 CAAGCGTGTG ACACAGAAAG AGGAGCTGGA ACGGCAGCGT GTGGAGCTAC AGCAGGAGGT
            661 GGAGAAGCTG GCTCGAGAGA ACAGCAGCAT GCGGCTGGAG CTAGATGCCC TGCGCTCCAA
            721 GTATGAGGCC CTACAGACCT TCGCTCGCAC CGTGGCCCGA NGGCCTGTCA CACCCACCNA
            781 GGTGGCCACC ACCAGTGTCA TCACCATCGT CAAATCTGCC GAGCTCTCCT CCNCCTCTGT
            841 ACCCTTCTCA GCCGCCTCCN ANTCNAGANG GNCCCCCCCT CGANGNCNAN GAANTCCNCC
          901 CCCCCCCCCN NNNCNNNNNN NNNN
          //
          Primer: IRES-R
          Region: IRES,insert3',FLAG,CAG pro
          Sequence file: RDB15412_A7J2b.seq check
          >D04907A1_A7J2_1_IRES-R_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT NAGGGGGGGG GGNNGGANAG GGGGGGGGGG
             61 CGGAATTCCT CGACCTCGAG GGGGGGCCCT CTAGACTAGG AGGCGGCTGA GAAGGGTACA
            121 GAGGTGGAGG AGAGCTCGGC AGATTTGACG ATGGTGATGA CACTGGTGGT GGCCACCTTG
            181 GTGGGTGTGA CAGGCCCTCG GGCCACGGTG CGAGCGAAGG TCTGTAGGGC CTCATACTTG
            241 GAGCGCAGGG CATCTAGCTC CAGCCGCATG CTGCTGTTCT CTCGAGCCAG CTTCTCCACC
            301 TCCTGCTGTA GCTCCACACG CTGCCGTTCC AGCTCCTCTT TCTGTGTCAC ACGCTTGATG
            361 CGACAGCTAG CCGCGTAGCC TCTGTTCTTG AGTGTGCGCC GCCGCTGCTT CAGCCGAGTG
            421 ACCTCCTCCT TGGTGAGCCC CCGCAGGTGC TGGTTTAGCT CCCGCACTGA CATGGACACC
            481 AGCTCATCAT CGCTAAGAAC AGGGGCGTTC TCACCCGCCT CCTTCTTGAC CTTCAATGCC
            541 TTGTTGGGCT TGGGATTAGT CGTGAATTCG CCCTTGTCAT CATCGTCCTT GTAGTCCATG
            601 GCGGTGGATC TGAATTCTTT GCCAAAATGA TGANACAGCA CAATAACCAG CACGTTGCCC
            661 AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTCT AGAGCCGCCG
            721 GTCACACGCC ANAAGCCGAA CCCCGCCCTG CCCCGTCCCC CCCGAANGCA GCCGTCCCCC
            781 CGCGGANAGC CCCGANGCTG GANAGGGAGA ANGGGANGGC GGCGCGGCGA CGCACGAANG
            841 CCCTCCCCGC CCATTTCCTT CCTGCCGGCG CCGCACCGCT TCGCCCCGCG CCNGCTANAG
            901 GGGGNGCGGC GGCGCCNNCC NNATTTNGGN TCCNCACANA TTTNGGNANA AAGGAAGTCC
            961 CTGCNCCCNN TCNNACNATT ACCATAAANN GNNANNGGNN GNNNNNCNNN GCGCNTCGNN
          1021 NGNNNNCNGG NNCNNNNGGG NNNNNNNNNN
          //
          Primer: IRES-F
          Region: IRES,PuroR
          Sequence file: RDB15412_A7J2c.seq check
          >D04907A1_A7J2_1_IRES-F_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNCGAGG TTANNAAACG TCTAGGCCCC CCGAACCNCG
             61 GGGACGTGGT TTTCCTTTGN NAAACACGAT GATAATATGG CCACAACCAT GACCGAGTAC
            121 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC
            181 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG
            241 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG
            301 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG
            361 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC
            421 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC
            481 CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG
            541 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG
            601 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG
            661 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC
            721 GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCNTG CATCGATGAT CTAGAGCTCG
            781 CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT
            841 GCCTTCCTTG ACCCTGNAAN GTGCCNCTCC CACTGTCCTT TCCTAANNAA ATGAGGAANN
            901 TGCNTCGCAT TGTCTGANTA NGTGTCATTC TATTCTGGGG GGGNGGGGTG GGGGCANGAC
            961 ANCAAGGGGG ANGANTGGGN AAGNNAATAN CNNNNTGCTG GGGNNGCNGT GGGCNNNANN
          1021 GNNNTNNNNA NNCNGNAAAN NNNNNGNAGC CNNNNNNNNN N
          //
          Primer: Amp_R
          Region: Polyoma Ori,SV40 poly(A),HCMVIEE
          Sequence file: RDB15412_A7J2d.seq check
          >D04907A1_A7J2_1_Amp_R_A04_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNAGANTGT ATTTCCTGGA AATTAATGTT
             61 TATTCNATAA ACTGTGTATT CAGCTATATT CACATAGTGG TGAGGCTGAA ATGAGGCGGG
            121 AAGAGGCGGT TGGGGCTTAA TTATATCAAT TTGGGTGGCC CCACAGCGCC TCCAAGGCGC
            181 CAGTCCTGTT TTGACAAGTT GCCTCTGGAA GCCTCTCTTC TTTTTCTCCA GAGTAAGCGG
            241 AGGCCAGGGG CCCCCGGCCT CTGCTTAATA CTAAAAAAAA CAGCTGTTGT CATAGTAATG
            301 ATTGGGTGGA AACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA
            361 CACTGACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGC
            421 CCTCTGGAGG GCAGTTGCCT AGCAACTAAT TAAAAGAGGA TGTCGCACGG CCAGCTGCGG
            481 TCAGTTAGTC ACTTCCTGCT TAACTGACTT GACATTTTCT ATTTTAAGAG TCGGGAGGAA
            541 AATTACTGTG TTGGAGGCCC TCCGCCATCT TCTGAAGCTG AATCGAATTA ACTTGTTTAT
            601 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT
            661 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG
            721 GATCTGATAT CATCGTCGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
            781 TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG
            841 CCTGGCTGAN CGNCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGNA TGTTCCCNTA
            901 GTAACGCCNA TANGGACTTN NNATTGACGT CAANNGGNGG AGTATTTANG GNAAACTGCC
            961 NNCTTGGNAN TANNTCAANN GTATCATATG CNNNGNANNN CCCCNANNGN NNNNNNTGAN
          1021 NGNNAANNNN NNNNNCNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Okita, Y., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl