Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAGIP-FLAG-Mafk

Expression vector of mouse Mafk.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017).
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017)。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15412_A7J2p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 Region: CAG pro,FLAG,insert5' Sequence file: RDB15412_A7J2a.seq
>D04907A1_A7J2_1_pCAG-F_V2_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NNNNTCTGGG NNGNGCCGCC GCACCCCCTC    61 TAGCGGGCGC GGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT   121 TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG CCTCGGGGCT GTCCGCGGGG   181 GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC GGCTTCTGGC GTGTGACCGG   241 CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG   301 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCAGAT CCACCGCCAT   361 GGACTACAAG GACGATGATG ACAAGGGCGA ATTCACGACT AATCCCAAGC CCAACAAGGC   421 ATTGAAGGTC AAGAAGGAGG CGGGTGAGAA CGCCCCTGTT CTTAGCGATG ATGAGCTGGT   481 GTCCATGTCA GTGCGGGAGC TAAACCAGCA CCTGCGGGGG CTCACCAAGG AGGAGGTCAC   541 TCGGCTGAAG CAGCGGCGGC GCACACTCAA GAACAGAGGC TACGCGGCTA GCTGTCGCAT   601 CAAGCGTGTG ACACAGAAAG AGGAGCTGGA ACGGCAGCGT GTGGAGCTAC AGCAGGAGGT   661 GGAGAAGCTG GCTCGAGAGA ACAGCAGCAT GCGGCTGGAG CTAGATGCCC TGCGCTCCAA   721 GTATGAGGCC CTACAGACCT TCGCTCGCAC CGTGGCCCGA NGGCCTGTCA CACCCACCNA   781 GGTGGCCACC ACCAGTGTCA TCACCATCGT CAAATCTGCC GAGCTCTCCT CCNCCTCTGT   841 ACCCTTCTCA GCCGCCTCCN ANTCNAGANG GNCCCCCCCT CGANGNCNAN GAANTCCNCC 901 CCCCCCCCCN NNNCNNNNNN NNNN //
Primer: IRES-R Region: IRES,insert3',FLAG,CAG pro Sequence file: RDB15412_A7J2b.seq
>D04907A1_A7J2_1_IRES-R_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT NAGGGGGGGG GGNNGGANAG GGGGGGGGGG    61 CGGAATTCCT CGACCTCGAG GGGGGGCCCT CTAGACTAGG AGGCGGCTGA GAAGGGTACA   121 GAGGTGGAGG AGAGCTCGGC AGATTTGACG ATGGTGATGA CACTGGTGGT GGCCACCTTG   181 GTGGGTGTGA CAGGCCCTCG GGCCACGGTG CGAGCGAAGG TCTGTAGGGC CTCATACTTG   241 GAGCGCAGGG CATCTAGCTC CAGCCGCATG CTGCTGTTCT CTCGAGCCAG CTTCTCCACC   301 TCCTGCTGTA GCTCCACACG CTGCCGTTCC AGCTCCTCTT TCTGTGTCAC ACGCTTGATG   361 CGACAGCTAG CCGCGTAGCC TCTGTTCTTG AGTGTGCGCC GCCGCTGCTT CAGCCGAGTG   421 ACCTCCTCCT TGGTGAGCCC CCGCAGGTGC TGGTTTAGCT CCCGCACTGA CATGGACACC   481 AGCTCATCAT CGCTAAGAAC AGGGGCGTTC TCACCCGCCT CCTTCTTGAC CTTCAATGCC   541 TTGTTGGGCT TGGGATTAGT CGTGAATTCG CCCTTGTCAT CATCGTCCTT GTAGTCCATG   601 GCGGTGGATC TGAATTCTTT GCCAAAATGA TGANACAGCA CAATAACCAG CACGTTGCCC   661 AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTCT AGAGCCGCCG   721 GTCACACGCC ANAAGCCGAA CCCCGCCCTG CCCCGTCCCC CCCGAANGCA GCCGTCCCCC   781 CGCGGANAGC CCCGANGCTG GANAGGGAGA ANGGGANGGC GGCGCGGCGA CGCACGAANG   841 CCCTCCCCGC CCATTTCCTT CCTGCCGGCG CCGCACCGCT TCGCCCCGCG CCNGCTANAG   901 GGGGNGCGGC GGCGCCNNCC NNATTTNGGN TCCNCACANA TTTNGGNANA AAGGAAGTCC   961 CTGCNCCCNN TCNNACNATT ACCATAAANN GNNANNGGNN GNNNNNCNNN GCGCNTCGNN 1021 NGNNNNCNGG NNCNNNNGGG NNNNNNNNNN //
Primer: IRES-F Region: IRES,PuroR Sequence file: RDB15412_A7J2c.seq
>D04907A1_A7J2_1_IRES-F_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCGAGG TTANNAAACG TCTAGGCCCC CCGAACCNCG    61 GGGACGTGGT TTTCCTTTGN NAAACACGAT GATAATATGG CCACAACCAT GACCGAGTAC   121 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC   181 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG   241 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG   301 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG   361 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC   421 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC   481 CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG   541 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG   601 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG   661 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC   721 GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCNTG CATCGATGAT CTAGAGCTCG   781 CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT   841 GCCTTCCTTG ACCCTGNAAN GTGCCNCTCC CACTGTCCTT TCCTAANNAA ATGAGGAANN   901 TGCNTCGCAT TGTCTGANTA NGTGTCATTC TATTCTGGGG GGGNGGGGTG GGGGCANGAC   961 ANCAAGGGGG ANGANTGGGN AAGNNAATAN CNNNNTGCTG GGGNNGCNGT GGGCNNNANN 1021 GNNNTNNNNA NNCNGNAAAN NNNNNGNAGC CNNNNNNNNN N //
Primer: Amp_R Region: Polyoma Ori,SV40 poly(A),HCMVIEE Sequence file: RDB15412_A7J2d.seq
>D04907A1_A7J2_1_Amp_R_A04_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNAGANTGT ATTTCCTGGA AATTAATGTT    61 TATTCNATAA ACTGTGTATT CAGCTATATT CACATAGTGG TGAGGCTGAA ATGAGGCGGG   121 AAGAGGCGGT TGGGGCTTAA TTATATCAAT TTGGGTGGCC CCACAGCGCC TCCAAGGCGC   181 CAGTCCTGTT TTGACAAGTT GCCTCTGGAA GCCTCTCTTC TTTTTCTCCA GAGTAAGCGG   241 AGGCCAGGGG CCCCCGGCCT CTGCTTAATA CTAAAAAAAA CAGCTGTTGT CATAGTAATG   301 ATTGGGTGGA AACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA   361 CACTGACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGC   421 CCTCTGGAGG GCAGTTGCCT AGCAACTAAT TAAAAGAGGA TGTCGCACGG CCAGCTGCGG   481 TCAGTTAGTC ACTTCCTGCT TAACTGACTT GACATTTTCT ATTTTAAGAG TCGGGAGGAA   541 AATTACTGTG TTGGAGGCCC TCCGCCATCT TCTGAAGCTG AATCGAATTA ACTTGTTTAT   601 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT   661 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG   721 GATCTGATAT CATCGTCGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG   781 TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG   841 CCTGGCTGAN CGNCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGNA TGTTCCCNTA   901 GTAACGCCNA TANGGACTTN NNATTGACGT CAANNGGNGG AGTATTTANG GNAAACTGCC   961 NNCTTGGNAN TANNTCAANN GTATCATATG CNNNGNANNN CCCCNANNGN NNNNNNTGAN 1021 NGNNAANNNN NNNNNCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl