Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGIP-FLAG-Mafk

Expression vector of mouse Mafk.

Catalog number RDB15412
Resource name pCAGIP-FLAG-Mafk
Clone info. Expression vector of mouse musculoaponeurotic fibrosarcoma (MAF) oncogene family protein K (Mafk), tagged with FLAG at N-terminal, SV40 ori, IRES-puromycin resistance, CAG promoter.
Expression was confirmed by the depositor with immunoblotting. Please refer Niwa, H. et al., Nat. Genet. 24 (4): 372-376, 2000 for pCAGIP vector.
Vector backbone pCAGIP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Mouse Mafk cDNA
Depositor Kato, Mitsuyasu |
 
Other clones in our bank mouse Mafk (NCBI Gene 17135) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017).
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15412 pCAGIP-FLAG-Mafk DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Okita, Y., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15412_A7J2p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2
Region: CAG pro,FLAG,insert5'
Sequence file: RDB15412_A7J2a.seq check
>D04907A1_A7J2_1_pCAG-F_V2_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NNNNTCTGGG NNGNGCCGCC GCACCCCCTC
   61 TAGCGGGCGC GGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT
  121 TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG CCTCGGGGCT GTCCGCGGGG
  181 GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC GGCTTCTGGC GTGTGACCGG
  241 CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG
  301 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCAGAT CCACCGCCAT
  361 GGACTACAAG GACGATGATG ACAAGGGCGA ATTCACGACT AATCCCAAGC CCAACAAGGC
  421 ATTGAAGGTC AAGAAGGAGG CGGGTGAGAA CGCCCCTGTT CTTAGCGATG ATGAGCTGGT
  481 GTCCATGTCA GTGCGGGAGC TAAACCAGCA CCTGCGGGGG CTCACCAAGG AGGAGGTCAC
  541 TCGGCTGAAG CAGCGGCGGC GCACACTCAA GAACAGAGGC TACGCGGCTA GCTGTCGCAT
  601 CAAGCGTGTG ACACAGAAAG AGGAGCTGGA ACGGCAGCGT GTGGAGCTAC AGCAGGAGGT
  661 GGAGAAGCTG GCTCGAGAGA ACAGCAGCAT GCGGCTGGAG CTAGATGCCC TGCGCTCCAA
  721 GTATGAGGCC CTACAGACCT TCGCTCGCAC CGTGGCCCGA NGGCCTGTCA CACCCACCNA
  781 GGTGGCCACC ACCAGTGTCA TCACCATCGT CAAATCTGCC GAGCTCTCCT CCNCCTCTGT
  841 ACCCTTCTCA GCCGCCTCCN ANTCNAGANG GNCCCCCCCT CGANGNCNAN GAANTCCNCC
  901 CCCCCCCCCN NNNCNNNNNN NNNNCNNNNN NNNGNTNNCN GGNCNNAANN NNNTNNNNAT
  961 AANNNNNGNG NNCNNNNNNN NATATGNTNN TTTTNNNCNN NANNNNCCNN CTTTNNNNNG
 1021 NGANNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN N
//
Primer: IRES-R
Region: IRES,insert3',FLAG,CAG pro
Sequence file: RDB15412_A7J2b.seq check
>D04907A1_A7J2_1_IRES-R_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT NAGGGGGGGG GGNNGGANAG GGGGGGGGGG
   61 CGGAATTCCT CGACCTCGAG GGGGGGCCCT CTAGACTAGG AGGCGGCTGA GAAGGGTACA
  121 GAGGTGGAGG AGAGCTCGGC AGATTTGACG ATGGTGATGA CACTGGTGGT GGCCACCTTG
  181 GTGGGTGTGA CAGGCCCTCG GGCCACGGTG CGAGCGAAGG TCTGTAGGGC CTCATACTTG
  241 GAGCGCAGGG CATCTAGCTC CAGCCGCATG CTGCTGTTCT CTCGAGCCAG CTTCTCCACC
  301 TCCTGCTGTA GCTCCACACG CTGCCGTTCC AGCTCCTCTT TCTGTGTCAC ACGCTTGATG
  361 CGACAGCTAG CCGCGTAGCC TCTGTTCTTG AGTGTGCGCC GCCGCTGCTT CAGCCGAGTG
  421 ACCTCCTCCT TGGTGAGCCC CCGCAGGTGC TGGTTTAGCT CCCGCACTGA CATGGACACC
  481 AGCTCATCAT CGCTAAGAAC AGGGGCGTTC TCACCCGCCT CCTTCTTGAC CTTCAATGCC
  541 TTGTTGGGCT TGGGATTAGT CGTGAATTCG CCCTTGTCAT CATCGTCCTT GTAGTCCATG
  601 GCGGTGGATC TGAATTCTTT GCCAAAATGA TGANACAGCA CAATAACCAG CACGTTGCCC
  661 AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTCT AGAGCCGCCG
  721 GTCACACGCC ANAAGCCGAA CCCCGCCCTG CCCCGTCCCC CCCGAANGCA GCCGTCCCCC
  781 CGCGGANAGC CCCGANGCTG GANAGGGAGA ANGGGANGGC GGCGCGGCGA CGCACGAANG
  841 CCCTCCCCGC CCATTTCCTT CCTGCCGGCG CCGCACCGCT TCGCCCCGCG CCNGCTANAG
  901 GGGGNGCGGC GGCGCCNNCC NNATTTNGGN TCCNCACANA TTTNGGNANA AAGGAAGTCC
  961 CTGCNCCCNN TCNNACNATT ACCATAAANN GNNANNGGNN GNNNNNCNNN GCGCNTCGNN
 1021 NGNNNNCNGG NNCNNNNGGG NNNNNNNNNN NCCNNNCNNN NNNNNNNCNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNN
//
Primer: IRES-F
Region: IRES,PuroR
Sequence file: RDB15412_A7J2c.seq check
>D04907A1_A7J2_1_IRES-F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCGAGG TTANNAAACG TCTAGGCCCC CCGAACCNCG
   61 GGGACGTGGT TTTCCTTTGN NAAACACGAT GATAATATGG CCACAACCAT GACCGAGTAC
  121 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC
  181 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG
  241 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG
  301 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG
  361 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC
  421 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC
  481 CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG
  541 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG
  601 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG
  661 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC
  721 GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCNTG CATCGATGAT CTAGAGCTCG
  781 CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT
  841 GCCTTCCTTG ACCCTGNAAN GTGCCNCTCC CACTGTCCTT TCCTAANNAA ATGAGGAANN
  901 TGCNTCGCAT TGTCTGANTA NGTGTCATTC TATTCTGGGG GGGNGGGGTG GGGGCANGAC
  961 ANCAAGGGGG ANGANTGGGN AAGNNAATAN CNNNNTGCTG GGGNNGCNGT GGGCNNNANN
 1021 GNNNTNNNNA NNCNGNAAAN NNNNNGNAGC CNNNNNNNNN NNNNNNNNNN NGNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNANNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
//
Primer: Amp_R
Region: Polyoma Ori,SV40 poly(A),HCMVIEE
Sequence file: RDB15412_A7J2d.seq check
>D04907A1_A7J2_1_Amp_R_A04_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNAGANTGT ATTTCCTGGA AATTAATGTT
   61 TATTCNATAA ACTGTGTATT CAGCTATATT CACATAGTGG TGAGGCTGAA ATGAGGCGGG
  121 AAGAGGCGGT TGGGGCTTAA TTATATCAAT TTGGGTGGCC CCACAGCGCC TCCAAGGCGC
  181 CAGTCCTGTT TTGACAAGTT GCCTCTGGAA GCCTCTCTTC TTTTTCTCCA GAGTAAGCGG
  241 AGGCCAGGGG CCCCCGGCCT CTGCTTAATA CTAAAAAAAA CAGCTGTTGT CATAGTAATG
  301 ATTGGGTGGA AACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA
  361 CACTGACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGC
  421 CCTCTGGAGG GCAGTTGCCT AGCAACTAAT TAAAAGAGGA TGTCGCACGG CCAGCTGCGG
  481 TCAGTTAGTC ACTTCCTGCT TAACTGACTT GACATTTTCT ATTTTAAGAG TCGGGAGGAA
  541 AATTACTGTG TTGGAGGCCC TCCGCCATCT TCTGAAGCTG AATCGAATTA ACTTGTTTAT
  601 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT
  661 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG
  721 GATCTGATAT CATCGTCGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
  781 TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG
  841 CCTGGCTGAN CGNCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGNA TGTTCCCNTA
  901 GTAACGCCNA TANGGACTTN NNATTGACGT CAANNGGNGG AGTATTTANG GNAAACTGCC
  961 NNCTTGGNAN TANNTCAANN GTATCATATG CNNNGNANNN CCCCNANNGN NNNNNNTGAN
 1021 NGNNAANNNN NNNNNCNNNN NNNNNNNNNN NNNNNNNNNN NCNNNNNNGG NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Okita, Y., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538.

2019.12.09

GNP_filter3_RDBDEP_html_191130.pl