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pUC-mdv-alphal180-mutL2 (#RDB15410)

Plasmid clone of in vitro RNA transcription of alpha-domain gene for the selection of repof beta-galactosidaselicable RNA.

Clone info. Plasmid clone of in vitro RNA transcription of alpha-domain gene for the selection of repof beta-galactosidaselicable RNA. Please refer Fig.1 of Yumura, M. et al. PLoS One, 12 (3): e0174130, 2017, for the scheme of the selection cycle.
Comment pUC-mdv-alpha_mutA vector was constructed from pUC19 (Usui, K. et al. Nucleic Acids Res., 43 (16): 8033-80343, 2015.).
Vector backbone pUC-mdv-alpha_mutA (Plasmid)
Selectable markers Amp^r
Gene/insert name E. coli beta-galactosidase genomic DNA
Depositor|Developer Ichihashi, Norikazu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yumura, M et al. PLoS One, 12 (3), e0174130, 2017). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yumura, M et al. PLoS One, 12 (3), e0174130, 2017)。非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。

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RDB15410 pUC-mdv-alphal180-mutL2 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pUC-mdv-alphal180-mutL2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15410).

Reference section:

Yumura, M., Yamamoto, N., Yokoyama, K., Mori, H., Yomo, T., Ichihashi, N., Combinatorial selection for replicable RNA by Qbeta replicase while maintaining encoded gene function. PLoS One 12 (3): e0174130 (2017). PMID 28328998. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Yumura, M., Combinatorial selection for replicable RNA by Qbeta replicase while maintaining encoded gene function. PLoS One 12 (3): e0174130 (2017). PMID 28328998. [link to RRC of NBRP]
reference Usui, K., A design principle for a single-stranded RNA genome that replicates with less double-strand formation. Nucleic Acids Res. 43 (16): 8033-8043 (2015). PMID 26202975.