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pUC-mdv-alphal180-mutL2

Plasmid clone of in vitro RNA transcription of alpha-domain gene for the selection of repof beta-galactosidaselicable RNA.

Catalog number RDB15410
Resource name pUC-mdv-alphal180-mutL2
Clone info. Plasmid clone of in vitro RNA transcription of alpha-domain gene for the selection of repof beta-galactosidaselicable RNA. Please refer Fig.1 of Yumura, M. et al. PLoS One, 12 (3): e0174130, 2017, for the scheme of the selection cycle.
pUC-mdv-alpha_mutA vector was constructed from pUC19 (Usui, K. et al. Nucleic Acids Res., 43 (16): 8033-80343, 2015.).
Vector backbone pUC-mdv-alpha_mutA (Plasmid)
Selectable markers Amp^r
Gene/insert name E. coli beta-galactosidase genomic DNA
Depositor Ichihashi, Norikazu |
 
Other clones in our bank E. coli lacZ (KEGG orthology K01190) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yumura, M et al. PLoS One, 12 (3), e0174130, 2017). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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RDB15410 pUC-mdv-alphal180-mutL2 Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Yumura, M., Combinatorial selection for replicable RNA by Qbeta replicase while maintaining encoded gene function. PLoS One 12 (3): e0174130 (2017). PMID 28328998.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Yumura, M., Combinatorial selection for replicable RNA by Qbeta replicase while maintaining encoded gene function. PLoS One 12 (3): e0174130 (2017). PMID 28328998.
reference Usui, K., A design principle for a single-stranded RNA genome that replicates with less double-strand formation. Nucleic Acids Res. 43 (16): 8033-8043 (2015). PMID 26202975.

2019.07.17

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