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pUC-mdv-alphal180-mutL2

Plasmid clone of in vitro RNA transcription of alpha-domain gene for the selection of repof beta-galactosidaselicable RNA.

Catalog number RDB15410
Resource name pUC-mdv-alphal180-mutL2
Clone info. Plasmid clone of in vitro RNA transcription of alpha-domain gene for the selection of repof beta-galactosidaselicable RNA. Please refer Fig.1 of Yumura, M. et al. PLoS One, 12 (3): e0174130, 2017, for the scheme of the selection cycle.
Comment pUC-mdv-alpha_mutA vector was constructed from pUC19 (Usui, K. et al. Nucleic Acids Res., 43 (16): 8033-80343, 2015.).
Vector backbone pUC-mdv-alpha_mutA (Plasmid)
Selectable markers Amp^r
Gene/insert name E. coli beta-galactosidase genomic DNA
Depositor|Developer Ichihashi, Norikazu |
Other clones in our bank
External Database
E. coli beta-galactosidase
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yumura, M et al. PLoS One, 12 (3), e0174130, 2017). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yumura, M et al. PLoS One, 12 (3), e0174130, 2017)。非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB15410 pUC-mdv-alphal180-mutL2 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Yumura, M., Combinatorial selection for replicable RNA by Qbeta replicase while maintaining encoded gene function. PLoS One 12 (3): e0174130 (2017). PMID 28328998.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Yumura, M., Combinatorial selection for replicable RNA by Qbeta replicase while maintaining encoded gene function. PLoS One 12 (3): e0174130 (2017). PMID 28328998.
          reference Usui, K., A design principle for a single-stranded RNA genome that replicates with less double-strand formation. Nucleic Acids Res. 43 (16): 8033-8043 (2015). PMID 26202975.

          2022.05.17

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