RDB15409 - pBaitD-gap43-linker-EGFP

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icon below as well as clone information before placing your order.

pBaitD-gap43-linker-EGFP (#RDB15409)

Donor plasmid for targeted insertion into the gap43 locus of Japanese medaka by the BaitD system.

Clone info.	Donor plasmid for targeted insertion of fluorescent protein marker gene to growth associated protein 43 (gap43) locus of Japanese medaka (Oryzias latipes) by the BaitD system. See Fig.3a of Murakami, Y. et al. Zoological Lett. 3: 10 (2017).
Comment	Expression was confirmed by the depositor with microscopic observation. Ensembl gene number ENSORLG00000015837
Vector backbone	Plasmid
Gene/insert name	Oryzias latipes gap43 genomic DNA Aequorea victoria GFP cDNA
Depositor\|Developer	Kinoshita, Masato \|

Sequence (full)	RDB15409zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Murakami, Y. et al. Zool. Letters 3: 10, 2017).

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Murakami, Y. et al. Zool. Letters 3: 10, 2017).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Murakami, Y. et al. Zool. Letters 3: 10, 2017)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15409	pBaitD-gap43-linker-EGFP	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBaitD-gap43-linker-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15409).

Reference section:

Murakami, Y., Ansai, S., Yonemura, A., Kinoshita, M., An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Zoological Lett. 3: 10 (2017). PMID 28694996. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15409_A7Ikp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_C_F (Pr0271) Region: AmpR,AmpR ter,Bait7,GAP43 intron1,exon2,linker Sequence file: RDB15409_A7Ikd.seq
>D04849A1_A7lk_1_Amp_C_F_A05_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTC GNANTTANCT ACNCGACGGG GNNTCAGGCA ACTATGGATG 61 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG 121 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA 181 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGAGCGGATA CACGTAGTGG ATCTTCGGCC 241 TAGACTGCGA GGCTCGAGGA ACATCTCAGT CTAAGACTCC ATTTTTCTTA GCAAATGTAC 301 ATTATAGCTA CAAAAGGTTC CTCTGGACCC ACATGACTGC TGCTAGCAGC TAATATTCTG 361 TACTGTGTGT TGTTCATCAG GTGGAGAAAA ATGAGGATGC AGACCAGAAG ATTGAGCAGG 421 ATGGCACCGC CAACAAACCC GAGGACAACA AGGCCCACAA GGCAGCCACC AAAATACAGG 481 CTAGCTTCCG TGGACACATA ACTCGGAAAA AGATGAAAGA CGGAGAGGAG GAGAAGGAAG 541 AAAACAGTCC TGTTGCAGAT GAGGCAAAAG AGAGAGAAGA GGAGACAAAG AAAGCAGAGG 601 GAGATGAGAC ACCTGCTAAG CAGGATGAAG CTGCAGGAGA GGAGGCGAAG GAAGAGTTAA 661 ACCAAGCCAA AAGCCCTGCA GCAGACAAAC CAGCTAACTC TCCGGCAGCT GCTGTCTCTC 721 CTGCAGGCGC AGCCACTTCT CCCGGATCCG GANGTAGCGG TGGAACTATG GTGAGCAAGG 781 GCGAGGANCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA NCTGGACGGC GACGTAAACG 841 GCCACAAGTT CAGCGTGTCC GGCGNNGGCG ANGNCGANGC CANCNACGGC AAGCTGANCC 901 TGAANTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCNN GCCNACCNNC NNGNNCNCCN 961 NNNNCNACNG NNNGCANNGN TNNAGCNGNN ACCCNNNNCN NNNNNAGCAN CNNNNNNNNN 1021 N //
Primer: SV40pA3element Reverse (Pr0108) Region: Bait7,GAP43 intron3,exon3,intron2,insert seq,exon2,SV40 pA Sequence file: RDB15409_A7Ike.seq
>D04849A1_A7lk_1_SV40pA3'ElementReverse_B05_05_ABI24.ab1 1 NNNNNNNNNN NNNNGNNNNN NTGGNNANNG CNNGCAACGC GGCCTTTTTA CGGTTCCTGG 61 CCTTTTGCTG GCCTTTTGCT GATCTTCGGC CTAGACTGCG AGGACTAGTC CTTATAAATG 121 CTTCACTATA CACTTACCAC ATGCACGACA AAGGTTTGTT CCATTTTCAT AAAGTCCCTT 181 AAGTTGGTTG TAGGACCCTA AAGGAAACTG CAAGACACAC CTGCTCTCCC CTTAGGATGC 241 AGCCACTCTT CTCTATGACC CTCTATGGGC CCGGATAACC AAAAAAACTG TAGCACCCAT 301 ACCGGTCATG TGACCAAGGC TTAACTGGAG CGCTGTAATC GGCATACCTT GTTGATCTTT 361 TGTTTGGTTA GGTTCTTCCT CAGCTGTTGG GCTGACAGCA GCAGGCACAT CGGCTTGTCT 421 GGCCTCCTCC TTCTTCTCCT CCCACTTTTT CTCCTCCTCC TTCTTCTCCT CAGCTGTGGC 481 TGTGGTGGGA CTCTTGGTTG TTGCAGCTGG AGCCTCCACC TCTTTGGGCT TCTCCTTCGG 541 CTCCTCTTTC TCAGGTTCAG AGGGCGCCGT GGCAGTTGTA GGAGCAGATG GAGCCGGCGC 601 CACAGGAATT CTAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA 661 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG 721 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGANGT 781 GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACNAATGT GGTATGGCTG ATTATGATCT 841 AGANTCGCGG CCGCTTTACT TGTACAGCTC GTCCATGCCG ANAGTGATNC CGGCGGCGGT 901 CNNGAACTCC AGCAGGNNCA TGTGATNGCG CTTCNCGTTG GGGTCTTTNC TCNNGGCNNN 961 NNNGGNGNNC NNNNANNGGN TNNNCNGGNA NCNNCNNNGG GGNNNNNNNN NN //
Primer: pGCAP_R (Pr0074) Region: GAP43 exon2,insert seq,intron2,exon3,intron3 Sequence file: RDB15409_A7Ikf.seq
>15409_15409_B2DdF_1_pGCAP_R_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNN CCGGCTCCNT CTGCTCCTAC NANCTGCCAC 61 GGCGCCCTCT GAACCTGAGA AAGAGGAGCC GAAGGAGAAG CCCAAAGAGG TGGAGGCTCC 121 AGCTGCAACA ACCAAGAGTC CCACCACAGC CACAGCTGAG GAGAAGAAGG AGGAGGAGAA 181 AAAGTGGGAG GAGAAGAAGG AGGAGGCCAG ACAAGCCGAT GTGCCTGCTG CTGTCAGCCC 241 AACAGCTGAG GAAGAACCTA ACCAAACAAA AGATCAACAA GGTATGCCGA TTACAGCGCT 301 CCAGTTAAGC CTTGGTCACA TGACCGGTAT GGGTGCTACA GTTTTTTTGG TTATCCGGGC 361 CCATAGAGGG TCATAGAGAA GAGTGGCTGC ATCCTAAGGG GAGAGCAGGT GTGTCTTGCA 421 GTTTCCTTTA GGGTCCTACA ACCAACTTAA GGGACTTTAT GAAAATGGAA CAAACCTTTG 481 TCGTGCATGT GGTAAGTGTA TAGTGAAGCA TTTATAAGGA CTANNCCTCG CAGTCTAGGC 541 CGAAGATCAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG 601 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG 661 TGGCGAANCC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG 721 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA 781 AGCGTGGCGC TTTCTCATAG CTCACGCTGT ANGTATCTCA GTTCGGNGNA NGNTCGTTCG 841 CTNCAAGCTG GGCTGTGTGC ACGANCCCCC CGTNNANCCC GANCGCTGCN CCTTNTCCNG 901 GNAACTATCN NNNNGAGTCN NANCCNGNNN ANNANNNNAN NTNNTCGCCN CNNGGCNNCN 961 NCCNNCNGGN NANNNNGNNN NNNNNANCNA GGNATGNNNG GNGGNGNNNN NNNANTTNNT 1021 TGNANNGGNG GNNNNNNCNN NCNGNTACNN NNNNANNNAN NNNNNNTTTN GNNNNCNGNN 1081 CTNNNNNGNA NNNCNNNNNN NNCNNNNNGA AAANNANNNN GNNNNNNNNN N //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3',SV40 pA,GAP43 exon2,insert seq Sequence file: RDB15409_A7Ikg.seq
>15409_15409_B2DdF_1_mVenus-M_F_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGTGAACT TNNAGANCCG CCNCNACATC 61 GAGGANGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC 121 CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC 181 AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC 241 GGCATGGACG AGCTGTACAA GTAAAGCGGC CGCGACTCTA GATCATAATC AGCCATACCA 301 CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC 361 ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 421 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 481 GTTTGTCCAA ACTCATCAAT GTATCTTAGA ATTCCTGTGG CGCCGGCTCC ATCTGCTCCT 541 ACAACTGCCA CGGCGCCCTC TGAACCTGAG AAAGAGGAGC CGAAGGAGAA GCCCAAAGAG 601 GTGGAGGCTC CAGCTGCAAC AACCAAGAGT CCCACNNNNN NCACAGCTGA GGAGAAGAAN 661 NNNNNGGAGA AAAAGTGGGA GGAGAAGAAG GAGGAGGCCA GACAAGCCGA TGTGCCTGCT 721 GCTGTCAGCC CAACAGCTGA GGAAGAACCT AACCNAACNA AAGATCAACA AGGTATGCCG 781 ATTACAGCGC TCCAGNTTAA ANCCTTGGTC ANNNGACCGG TATGGGTGCN ACAGNTTTTT 841 TTNGGTTATC CGGGCCCNNT AGAGGGTCNT AGANNANAGT GGNNTGCNNN CCTAANGNGA 901 NAGCNNGTGN GNNNTNGNAG TTTTCCNTNA NGNNCCTACN ANCAACTTNA NGGNANTTTN 961 NNAAAATGGA NNAANNNNTT TGNCNNGNNT GTGNNAAGTG NNNTAGTGAN NCATTNNNNN 1021 AGGNCTANNC NTNNNNNNNN NN //
Primer: EGFP-M_R (Pr0743) Region: EGFP 5',linker,GAP43 exon2 Sequence file: RDB15409_A7Ikh.seq
>15409_15409_B2DdF_1_EGFP-M_R_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NGNGNNGGNN CTTGAAGTTN NCCTTGATGC CGTTCTTCTG 61 CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT TGTGCCCCAG 121 GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC 181 GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT 241 GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG 301 GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTAGGTCAGG GTGGTCACGA GGGTGGGCCA 361 GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCAGG GTCAGCTTGC CGTAGGTGGC 421 ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC 481 GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA TAGTTCCACC 541 GCTACCTCCG GATCCGGGAG AAGTGGCTGC GCCTGCAGGA GAGACAGCAG CTGCCGGAGA 601 GTTAGCTGGT TTGTCTGCTG CAGGGCTTTT GGCTTGGTTT AACTCTTCCT TCGCCTCCTC 661 TCCTGCAGCT TCATCCTGCT TAGCAGGTGT CTCATCTCCC TCTGCTTTCT TTGTCTCCTC 721 TTCTCTCTCT TTTGCCTCAT CTGCAACANG ACTGTTTTCT TCCTTCTCCT CCTCTCCGTC 781 TTTCATCTTT TTCCGAGTTA TGTGTNNNNN GNNNNTAGCC TGTATTTTGG TGGCTGCCTT 841 GTGGNCCTTG TTGTCCTCGG GTTTGTTGNN GGNNGCCNNN NTGCTCNNTN NTTCTGGTCT 901 GCANNCTCAN TTTTCTNCNN CNGNANGAAC AACACACAGT ACANANNANT AGCTGNTAGC 961 ANCANNCNTG NGGGNTNCAN ANGAACNNTT TNGNNNNNTA TNNNGNNNNN TTTGCNNNNN 1021 AAAAATGNAA NTNTNNNANN NNANNNGTTN CNTCNNNNNT CGNNNNNNAN NNNNNNNANN 1081 NNNNNNNN //
Primer: FP-N_F1 (Pr0920) Region: EGFP 3',SV40 pA Sequence file: RDB15409_A7Iki.seq
>15409_15409_B2DdF_1_FP-N_F1_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCN TGAAGCAGCA CGACTTCTNN NNNNCCGCCA 61 TGCCCGAAGG CTACNNNNNN NANCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA 121 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA 181 TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC 241 ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC 301 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA 361 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA 421 GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG 481 GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGCGACTCT AGATCATAAT 541 CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT 601 GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTNAC TTGTTTATTG NAGCTTATAA 661 TGGTTACNAA TAAAGCAATA GCATCACAAA NTTNNCAAAT AAAGNATTTT TTTNNNCNNN 721 NNNNNANNNN NNNNTNNNNN NANNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Murakami, Y., An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Zoological Lett. 3: 10 (2017). PMID 28694996. [link to RRC of NBRP]
reference	Okuyama, T., Controlled Cre/loxP site-specific recombination in the developing brain in medaka fish, Oryzias latipes. PLoS One 8 (6): e66597 (2013). PMID 23825546.