Resource data sheet
DNA Bank Top

pBaitD-gap43-linker-EGFP (#RDB15409)

Donor plasmid for targeted insertion into the gap43 locus of Japanese medaka by the BaitD system.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Murakami, Y., Zoological Lett. 3: 10 (2017). PMID 28694996. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Donor plasmid for targeted insertion of fluorescent protein marker gene to growth associated protein 43 (gap43) locus of Japanese medaka (Oryzias latipes) by the BaitD system. See Fig.3a of Murakami, Y. et al. Zoological Lett. 3: 10 (2017).
Comment Expression was confirmed by the depositor with microscopic observation. Ensembl gene number ENSORLG00000015837
Vector backbone Plasmid
Growth conditions LB+Ampicilin, 37oC
Gene/insert name Oryzias latipes gap43 genomic DNA Aequorea victoria GFP cDNA
Depositor|Developer Kinoshita, Masato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Murakami, Y. et al. Zool. Letters 3: 10, 2017).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Murakami, Y. et al. Zool. Letters 3: 10, 2017)。

Catalog # Resource name Shipping form Fee
RDB15409 pBaitD-gap43-linker-EGFP DNA solution

How to cite this biological resource

Materials & Methods section:

The pBaitD-gap43-linker-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15409).

Reference section:

Murakami, Y., Ansai, S., Yonemura, A., Kinoshita, M., An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Zoological Lett. 3: 10 (2017). PMID 28694996. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Murakami, Y., An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Zoological Lett. 3: 10 (2017). PMID 28694996. [PubMed] [Article] [RRC of NBRP]
reference Okuyama, T., Controlled Cre/loxP site-specific recombination in the developing brain in medaka fish, Oryzias latipes. PLoS One 8 (6): e66597 (2013). PMID 23825546. [PubMed] [Article]