Resource data sheet
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pBaitD-gap43-linker-EGFP

Donor plasmid for targeted insertion into the gap43 locus of Japanese medaka by the BaitD system.

Catalog number RDB15409
Resource name pBaitD-gap43-linker-EGFP
Clone info. Donor plasmid for targeted insertion of fluorescent protein marker gene to growth associated protein 43 (gap43) locus of Japanese medaka (Oryzias latipes) by the BaitD system. See Fig.3a of Murakami, Y. et al. Zoological Lett. 3: 10 (2017).
Expression was confirmed by the depositor with microscopic observation. Ensembl gene number ENSORLG00000015837
Vector backbone Plasmid
Gene/insert name Oryzias latipes gap43 genomic DNA Aequorea victoria GFP cDNA
Depositor Kinoshita, Masato |
 
Other clones in our bank Oryzias latipes gap43 (KEGG orthology K20041) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Murakami, Y. et al. Zool. Letters 3: 10, 2017).
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Exclusive MTA (genome editing) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use the Exclusive MTA (genome editing). [Word]
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


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RDB15409 pBaitD-gap43-linker-EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Murakami, Y., An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Zoological Lett. 3: 10 (2017). PMID 28694996.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15409_A7Ikp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_C_F RDB15409_A7Ika.seq
>D04849A1_A7lk_1_Amp_C_F_A05_02_ABI24.ab1
    1 TTCAATGTAG CCTCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG
   61 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG
  121 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA
  181 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGAGCGGATA CACGTAGTGG ATCTTCGGCC
  241 TAGACTGCGA GGCTCGAGGA ACATCTCAGT CTAAGACTCC ATTTTTCTTA GCAAATGTAC
  301 ATTATAGCTA CAAAAGGTTC CTCTGGACCC ACATGACTGC TGCTAGCAGC TAATATTCTG
  361 TACTGTGTGT TGTTCATCAG GTGGAGAAAA ATGAGGATGC AGACCAGAAG ATTGAGCAGG
  421 ATGGCACCGC CAACAAACCC GAGGACAACA AGGCCCACAA GGCAGCCACC AAAATACAGG
  481 CTAGCTTCCG TGGACACATA ACTCGGAAAA AGATGAAAGA CGGAGAGGAG GAGAAGGAAG
  541 AAAACAGTCC TGTTGCAGAT GAGGCAAAAG AGAGAGAAGA GGAGACAAAG AAAGCAGAGG
  601 GAGATGAGAC ACCTGCTAAG CAGGATGAAG CTGCAGGAGA GGAGGCGAAG GAAGAGTTAA
  661 ACCAAGCCAA AAGCCCTGCA GCAGACAAAC CAGCTAACTC TCCGGCAGCT GCTGTCTCTC
  721 CTGCAGGCGC AGCCACTTCT CCCGGATCCG GAGGTAGCGG TGGAACTATG GTGAGCAAGG
  781 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
  841 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC
  901 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC
  961 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT
 1021 TCAAGTCCGG CCATGCCCGA AGGCTACGTC CCAGGAGCGC ACCCATCTTC TTCATGGACG
 1081 ACGGCAACTA CAGGACCGCG CCGGAGGTGA AGGCTCGAGG CGAACCACCC TGGTTGGAAC
1141 CGGCCAA
//
Primer: SV40pA3'Element Reverse RDB15409_A7Ikb.seq
>D04849A1_A7lk_1_SV40pA3'ElementReverse_B05_05_ABI24.ab1
    1 GTGTCGGGGG GGCGGAGCCT ATGGAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTTGCTG GCCTTTTGCT GATCTTCGGC CTAGACTGCG AGGACTAGTC CTTATAAATG
  121 CTTCACTATA CACTTACCAC ATGCACGACA AAGGTTTGTT CCATTTTCAT AAAGTCCCTT
  181 AAGTTGGTTG TAGGACCCTA AAGGAAACTG CAAGACACAC CTGCTCTCCC CTTAGGATGC
  241 AGCCACTCTT CTCTATGACC CTCTATGGGC CCGGATAACC AAAAAAACTG TAGCACCCAT
  301 ACCGGTCATG TGACCAAGGC TTAACTGGAG CGCTGTAATC GGCATACCTT GTTGATCTTT
  361 TGTTTGGTTA GGTTCTTCCT CAGCTGTTGG GCTGACAGCA GCAGGCACAT CGGCTTGTCT
  421 GGCCTCCTCC TTCTTCTCCT CCCACTTTTT CTCCTCCTCC TTCTTCTCCT CAGCTGTGGC
  481 TGTGGTGGGA CTCTTGGTTG TTGCAGCTGG AGCCTCCACC TCTTTGGGCT TCTCCTTCGG
  541 CTCCTCTTTC TCAGGTTCAG AGGGCGCCGT GGCAGTTGTA GGAGCAGATG GAGCCGGCGC
  601 CACAGGAATT CTAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA
  661 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG
  721 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT
  781 GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCT
  841 AGAGTCGCGG CCGCTTTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT
  901 CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA
  961 CTGGGTGCTC AGGTAGTGGT TTGTCGGGCA GCAGCACGGG GGCCGTCGCC GATGGGGGTG
 1021 TTTCTGCTGG TAGTGGGTCG GCGAGCTGCA CGCTGCCCGT CCCTCGAATG TTGTGGCGGA
 1081 TCTTGAAAGT TTCACCATGA TGCGATCCTC CTGCCGTGTC CGTCATGAAT AATAGAACGA
1141 TTGGGTTGGC CTGGAG
//
Primer: SV40polyA-R RDB15409_A7Ikc.seq
>D04849A1_A7lk_1_SV40polyA-R_D05_11_ABI24.ab1
    1 AAAAATGCAG TTTTTAAGCA GTAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG
   61 AGTCGCGGCC GCTTTACTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA
  121 CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT
  181 GGGTGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT
  241 GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT
  301 TCACCTTGAT GCCGTTCTTC TGCTTGTCGG CCATGATATA GACGTTGTGG CTGTTGTAGT
  361 TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT
  421 CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC
  481 CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA
  541 AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA GCACTGCACG CCGTAGGTCA
  601 GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATGAACTTCA
  661 GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC
  721 CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC
  781 CCTTGCTCAC CATAGTTCCA CCGCTACCTC CGGATCCGGG AGAAGTGGCT GCGCCTGCAG
  841 GAGAGACAGC AGCTGCCGGA GAGTTAGCTG GTTTGTCTGC TGCAGGGCTT TTGGCTTGGT
  901 TTAACTCTTC CTTCGCCTCC TCTCCTGCAG CTTCATCCTG CTTAGCAGGT GTCTCATCTC
  961 CCTCTGCTTT CTTTGTCTCC TCTTCTCTCT CTTTTGCCTC ATCTGCAACA GGACTGTTTT
 1021 CTTCCTTCTC CTCCTCTCCG TCTTTCATCT TTTTCCGAGT TATGTGTCCC ACGGGAAGCT
 1081 AGCCCTTGTA TTTTTGGGTG CTGCATGTGG ACTTGATGTC CTCGGATTGA TGGCGGATGG
1141 CATCTGCTCA ATCTCTGGAT CTGCCAAATC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Murakami, Y., An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Zoological Lett. 3: 10 (2017). PMID 28694996.
reference Okuyama, T., Controlled Cre/loxP site-specific recombination in the developing brain in medaka fish, Oryzias latipes. PLoS One 8 (6): e66597 (2013). PMID 23825546.

2018.08.31

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