Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMSCV-3xFLAG-mp62-del UBA

Retroviral expression vector of mouse p62 deletion mutant (Ub-associated (UBA) domain, 392-436 a.a) tagged with 3xFLAG at N-terminal.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15408_A7Ikp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: gag-M_F Region: gag3', 3xFLAG, insert5' Sequence file: RDB15408_A7Ika.seq
>D04516A8_A7Ik_1_gag-M_F_A03_03_ABI24.ab1     1 ATGTGGGTAG CCCTTTGTAA CCCTAAGCCT CCGCCTCCTC TTCCTCCATC CGCCCCGTCT    61 CTCCCCCTTG AACCTCCTCG TTCGACCCCG CCTCGATCCT CCCTTTATCC AGCCCTCACT   121 CCTTCTCTAG GCGCCGGAAT TAGATCCATG GATTACAAGG ATGACGACGA TAAGAGATCC   181 GATTACAAGG ATGACGACGA TAAGAGATCC GATTACAAGG ATGACGACGA TAAGAGATCC   241 CCCGGGCTGC AGGAATTCAT GGCGTCGTTC ACGGTGAAGG CCTATCTTCT GGGCAAGGAG   301 GAGGCGACCC GCGAGATCCG CCGCTTCAGC TTCTGCTTCA GCCCGGAGCC GGAGGCGGAA   361 GCCCAAGCCG CGGCCGGCCC GGGGCCCTGC GAGAGGCTGC TGAGCCGAGT GGCTGTGCTG   421 TTCCCCACGC TGAGGCCTGG CGGCTTCCAG GCGCACTACC GCGATGAGGA TGGGGACTTG   481 GTTGCCTTTT CCAGTGATGA GGAGCTGACA ATGGCTATGT CCTATGTGAA AGATGACATC   541 TTCCGCATCT ACATTAAAGA GAAGAAGGAG TGCCGGCGGG AACATCGCCC ACCATGTGCT   601 CAGGAGGCAC CCCGAAACAT GGTGCACCCC AATGTGATCT GTGATGGTTG CAACGGGCCT   661 GTGGTGGGAA CTCGCTATAA GTGCAGTGTG TGCCCAGACT ACGACCTGTG CAGCGTGTGC   721 GAGGGGAAGG GCCTGCACAG GGAACACAGC AAGCTCATCT TTCCCAACCC CTTTGGCCAC   781 CTCTCTGATA GCTTCTCTCA TAGCCGCTGG CTTCGGAAGC TGAAACATGG ACACTTTGGC   841 TGGCCTGGCT GGGAGATGGG CCCACCGGGG AACTGGAGCC CACGTCCTCC TCGTGCAGGG   901 GATGGCCGCC CTTGCCCTAC AGCTGAGTCA GCTTCTGCTC CACCAGAAGA TCCCAATGTC   961 AATTTCCTGA AGAATGTGGG GGGAGAGTGT GGCAGCTGCC CTCAGCCCTC TAGGCATTGA  1021 GGTTGACATT GATGTGCACA TGGAGGGAAG AGAGCCCGCC CTGACACCCA CTACCCCCAG  1081 AAAGTTTCCA GCCACAGCAC AGAGGACCAG AAGTTACACT CAGGCCAAGG CCAGCATGCC 1141 TTCTTTCG //
Primer: PGK_N_rev Region: PGK pro, insert3' Sequence file: RDB15408_A7Ikb.seq
>D04516A8_A7Ik_1_PGK_N_rev_B03_06_ABI24.ab1     1 CTGGTGGCGC TCCCTACCCG GTAGAATTCG TTAACCTCGA GTCACAATGG TGGAGGGTGC    61 TTAGATCCCT CTGGTGGGAG ATGTGGGTAT AGGGCAGCTT CCTTCAGCCC TGTGGGTCCT   121 TCCTGTGAGG GGTCTAGAGA GCTTGGCCCT TCCGATTCTG GCATCTGTAG AGACTGGAGT   181 TCACCTGTAG ATGGGTCCAC TTCTTTTGAA GACAAATGTG TCCAGTCATC GTCTCCTCCT   241 GAGCAGTTTC CCGACTCCAT CTGTTCCTCT GGCTGTCCCA CCGACTCCAA GGCTATCTTT   301 TTCATTTGCT CTGTCAGAGA CTGAGCAGGG CCCTCCCCAG CCCCGTCAGG TTTGCTGACT   361 TCCGAAGAGC AGCTGCTTGG CTGAGTGTTA CTCTTGTCTT CTGTGCCTGT GCTGGAACTT   421 TCTGGGGTAG TGGGTGTCAG GCGGCTTCTC TTCCCTCCAT GTTCCACATC AATGTCAACC   481 TCAATGCCTA GAGGGCTGAG GGCAGCTGCC ACACTCTCCC CCACATTCTT CAGGAAATTG   541 ACATTGGGAT CTTCTGGTGG AGCAGAAGCT GACTCAGCTG TAGGGCAAGG GCGGCCATCC   601 CCTGCACGAG GAGGACGTGG GCTCCAGTTC CCCGGTGGGC CCATCTCCCA GCCAGGCCAG   661 CCAAAGTGTC CATGTTTCAG CTTCCGAAGC CAGCGGCTAT GAGAGAAGCT ATCAGAGAGG   721 TGGCCAAAGG GGTTGGGAAA GATGAGCTTG CTGTGTTCCC TGTGCAGGCC CTTCCCCTCG   781 CACACGCTGC ACAGGTCGTA GTCTGGGCAC ACACTGCACT TATAGCGAGT TCCCACCACA   841 GGCCCGTTGC AACCATCACA GATCACATTG GGGTGCACCA TGTTTCGGGG TGCCTCCTGA   901 GCACATGGTG GGCGATGTTC CCGCCGGCAC TCCTTCTTCT CTTTAATGTA GATGCGGAAG   961 ATGTCATCTT TCACATAGGA CATAGCCATT GTCAGCTCCT CATCACTGGG AAAAGGGCAA  1021 CCAAGTCCCC ATCCTCATCG CGGGTAGTGC GCCTGCAAGC CGCCAGGGCC TCAGCCGTGG  1081 GGACAGCACA GCCACTCGAG CTCAGCAGCC TCTCGCAACG ACCCCGGACC GGACCGCCGG 1141 CCTTGGGGGC ATC //
Primer: pBR322_BamHI_R Region: 5'LTR, MESV psi Sequence file: RDB15408_A7Ikc.seq
>D04516A8_A7Ik_1_pBR322_BamHI_R_C03_09_ABI24.ab1     1 ATCTTAGGAC GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG    61 AAGCCTATAG AGTACGAGCC ATAGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG   121 GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC   181 AAGGCATGGA AAATACATAA CTGAGAATAG AGAAGTTCAG ATCAAGGTTA GGAACAGAGA   241 GACAGCAGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG   301 GCCAAGAACA GATGGTCCCC AGATGCGGTC CCGCCCTCAG CAGTTTCTAG AGAACCATCA   361 GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA   421 TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC   481 ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATAGACTGC GTCGCCCGGG TACCCGTATT   541 CCCAATAAAG CCTCTTGCTG TTTGCATCCG AATCGTGGAC TCGCTGATCC TTGGGAGGGT   601 CTCCTCAGAT TGATTGACTG CCCACCTCGG GGGTCTTTCA TTTGGAGGTT CCACCGAGAT   661 TTGGAGACCC CTGCCCAGGG ACCACCGACC CCCCCGCCGG GAGGTAAGCT GGCCAGCGGT   721 CGTTTCGTGT CTGTCTCTGT CTTTGTGCGT GTTTGTGCCG GCATCTAATG TTTGCGCCTG   781 CGTCTGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG AACTGACGAG   841 TTCTGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTTGGGGG GCCGTTTTTG   901 TGGCCCGACC TGAGGAAAGG GAGTCGATGT GGAATCCGAC CCCGTCAGGA TATGTGGGTT   961 CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCCTCCG TCTGAATTTT TGCTTTCGGG  1021 TTTGGAACCC GAAAGCCGCG CGTCTTGTCT GCTGCAGCGC TGCAGCATCG TTCTGTGATG  1081 TCTCTGTCTG ACTGTGTTTC TGTAATTTGG TCTGAAATAA CGGACAGGAC TGATTAACCA 1141 CCTCCCTTAA GTATGGACCA TTAG //
Primer: PGKpro_F Region: PGK pro, HygR 5' Sequence file: RDB15408_A7Ikd.seq
>D04516A8_A7Ik_1_PGKpro_F_E03_15_ABI24.ab1     1 ACTGGGCGAT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCAGCCCAAG CTTATGAAAA    61 AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT   121 CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG   181 GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG   241 TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAGT   301 TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC   361 TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG   421 CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC   481 AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC   541 AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC   601 TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA   661 ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG   721 GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG   781 AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC   841 GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT   901 TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA   961 CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGGCCG TCTGGGACCG ATGGGCTGTG  1021 TAGAAGTACT CGCCCGATAG TGGAAACGGA CGCCCCAGCA CTCGTCCGAA GGCCAACGAT  1081 AGATCGATAA AATAAAGATT TATTTAGTCT CAGAAAACGG GGGGAATGAA AGAACCCTCA 1141 ACC //
Primer: HygR-C_F Region: HygR 3', 3'LTR Sequence file: RDB15408_A7Ike.seq
>D04516A8_A7Ik_1_HygR-C_F_H03_08_ABI08.ab1     1 CGAAACGGGG GGATCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT    61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG   121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC   181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA   241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC   301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG   361 GAATAGATCG ATAAAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA   421 CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAT   481 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTTAGGAA CAGAGAGACA GCAGAATATG   541 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG   601 GTCCCCAGAT GCGGTCCCGC CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT   661 GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC   721 GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC   781 GGCGCGCCAG TCCTCCGATA GACTGCGTCG CCCGGGGTAC CCGTGTATCC AATAAACCCT   841 CTTGCAGTTG CATCCGACTT GTGGTCTCGC TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT   901 TGACTACCCG TCAGCGGGGG GTCTTTTCAT GGGGTAACAG TTTCTTGAAG TTTGGAGAAA   961 CAACATTTCT GAGGGGTAGG GAGTCGAAAT ATTAAGTAAA TCCCTGACTC AATTTAGCCC  1021 ACTGTTTTGA ATCGACATAC TCCCATACTC CCTTGAATTA GTTTCCATAT GGACAGCGCA 1081 GAAGAAGCCT GGGGAGAACT TGCTGCAAAT TGGTCATC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl