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pMSCV-3xFLAG-mp62-del UBA (#RDB15408)

Retroviral expression vector of mouse p62 deletion mutant (Ub-associated (UBA) domain, 392-436 a.a) tagged with 3xFLAG at N-terminal.

Clone info. Retroviral expression vector of mouse p62 deletion mutant (Ub-associated (UBA) domain, 392-436 a.a) tagged with 3xFLAG at N-terminal. See Fig.5e of Watanabe, Y. et al. Nat. Commun., 7: 13508, 2016.
Comment Expression was confirmed by the depositor with immunostaining and western blotting.
Vector backbone pMSCV–Flag(3x) (plasmid)
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells).
Gene/insert name mouse p62 cDNA
Depositor|Developer Shimizu, Shigeomi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15408 pMSCV-3xFLAG-mp62-del UBA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMSCV-3xFLAG-mp62-del UBA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15408).

Reference section:

Watanabe, Y., Honda, S., Konishi, A., Arakawa, S., Murohashi, M., Yamaguchi, H., Torii, S., Tanabe, M., Tanaka, S., Warabi, E., Shimizu, S., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15408_A7Ikp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: gag-M_F (Pr0730)
Region: gag3', 3xFLAG, insert5'
Sequence file: RDB15408_A7Ika.seq check
>D04516A8_A7Ik_1_gag-M_F_A03_03_ABI24.ab1
    1 ATGTGGGTAG CCCTTTGTAA CCCTAAGCCT CCGCCTCCTC TTCCTCCATC CGCCCCGTCT
   61 CTCCCCCTTG AACCTCCTCG TTCGACCCCG CCTCGATCCT CCCTTTATCC AGCCCTCACT
  121 CCTTCTCTAG GCGCCGGAAT TAGATCCATG GATTACAAGG ATGACGACGA TAAGAGATCC
  181 GATTACAAGG ATGACGACGA TAAGAGATCC GATTACAAGG ATGACGACGA TAAGAGATCC
  241 CCCGGGCTGC AGGAATTCAT GGCGTCGTTC ACGGTGAAGG CCTATCTTCT GGGCAAGGAG
  301 GAGGCGACCC GCGAGATCCG CCGCTTCAGC TTCTGCTTCA GCCCGGAGCC GGAGGCGGAA
  361 GCCCAAGCCG CGGCCGGCCC GGGGCCCTGC GAGAGGCTGC TGAGCCGAGT GGCTGTGCTG
  421 TTCCCCACGC TGAGGCCTGG CGGCTTCCAG GCGCACTACC GCGATGAGGA TGGGGACTTG
  481 GTTGCCTTTT CCAGTGATGA GGAGCTGACA ATGGCTATGT CCTATGTGAA AGATGACATC
  541 TTCCGCATCT ACATTAAAGA GAAGAAGGAG TGCCGGCGGG AACATCGCCC ACCATGTGCT
  601 CAGGAGGCAC CCCGAAACAT GGTGCACCCC AATGTGATCT GTGATGGTTG CAACGGGCCT
  661 GTGGTGGGAA CTCGCTATAA GTGCAGTGTG TGCCCAGACT ACGACCTGTG CAGCGTGTGC
  721 GAGGGGAAGG GCCTGCACAG GGAACACAGC AAGCTCATCT TTCCCAACCC CTTTGGCCAC
  781 CTCTCTGATA GCTTCTCTCA TAGCCGCTGG CTTCGGAAGC TGAAACATGG ACACTTTGGC
  841 TGGCCTGGCT GGGAGATGGG CCCACCGGGG AACTGGAGCC CACGTCCTCC TCGTGCAGGG
  901 GATGGCCGCC CTTGCCCTAC AGCTGAGTCA GCTTCTGCTC CACCAGAAGA TCCCAATGTC
  961 AATTTCCTGA AGAATGTGGG GGGAGAGTGT GGCAGCTGCC CTCAGCCCTC TAGGCATTGA
 1021 GGTTGACATT GATGTGCACA TGGAGGGAAG AGAGCCCGCC CTGACACCCA CTACCCCCAG
 1081 AAAGTTTCCA GCCACAGCAC AGAGGACCAG AAGTTACACT CAGGCCAAGG CCAGCATGCC
1141 TTCTTTCG
//
Primer: PGK_N_rev (Pr0243)
Region: PGK pro, insert3'
Sequence file: RDB15408_A7Ikb.seq check
>D04516A8_A7Ik_1_PGK_N_rev_B03_06_ABI24.ab1
    1 CTGGTGGCGC TCCCTACCCG GTAGAATTCG TTAACCTCGA GTCACAATGG TGGAGGGTGC
   61 TTAGATCCCT CTGGTGGGAG ATGTGGGTAT AGGGCAGCTT CCTTCAGCCC TGTGGGTCCT
  121 TCCTGTGAGG GGTCTAGAGA GCTTGGCCCT TCCGATTCTG GCATCTGTAG AGACTGGAGT
  181 TCACCTGTAG ATGGGTCCAC TTCTTTTGAA GACAAATGTG TCCAGTCATC GTCTCCTCCT
  241 GAGCAGTTTC CCGACTCCAT CTGTTCCTCT GGCTGTCCCA CCGACTCCAA GGCTATCTTT
  301 TTCATTTGCT CTGTCAGAGA CTGAGCAGGG CCCTCCCCAG CCCCGTCAGG TTTGCTGACT
  361 TCCGAAGAGC AGCTGCTTGG CTGAGTGTTA CTCTTGTCTT CTGTGCCTGT GCTGGAACTT
  421 TCTGGGGTAG TGGGTGTCAG GCGGCTTCTC TTCCCTCCAT GTTCCACATC AATGTCAACC
  481 TCAATGCCTA GAGGGCTGAG GGCAGCTGCC ACACTCTCCC CCACATTCTT CAGGAAATTG
  541 ACATTGGGAT CTTCTGGTGG AGCAGAAGCT GACTCAGCTG TAGGGCAAGG GCGGCCATCC
  601 CCTGCACGAG GAGGACGTGG GCTCCAGTTC CCCGGTGGGC CCATCTCCCA GCCAGGCCAG
  661 CCAAAGTGTC CATGTTTCAG CTTCCGAAGC CAGCGGCTAT GAGAGAAGCT ATCAGAGAGG
  721 TGGCCAAAGG GGTTGGGAAA GATGAGCTTG CTGTGTTCCC TGTGCAGGCC CTTCCCCTCG
  781 CACACGCTGC ACAGGTCGTA GTCTGGGCAC ACACTGCACT TATAGCGAGT TCCCACCACA
  841 GGCCCGTTGC AACCATCACA GATCACATTG GGGTGCACCA TGTTTCGGGG TGCCTCCTGA
  901 GCACATGGTG GGCGATGTTC CCGCCGGCAC TCCTTCTTCT CTTTAATGTA GATGCGGAAG
  961 ATGTCATCTT TCACATAGGA CATAGCCATT GTCAGCTCCT CATCACTGGG AAAAGGGCAA
 1021 CCAAGTCCCC ATCCTCATCG CGGGTAGTGC GCCTGCAAGC CGCCAGGGCC TCAGCCGTGG
 1081 GGACAGCACA GCCACTCGAG CTCAGCAGCC TCTCGCAACG ACCCCGGACC GGACCGCCGG
1141 CCTTGGGGGC ATC
//
Primer: pBR322_BamHI_R (Pr0065)
Region: 5'LTR, MESV psi
Sequence file: RDB15408_A7Ikc.seq check
>D04516A8_A7Ik_1_pBR322_BamHI_R_C03_09_ABI24.ab1
    1 ATCTTAGGAC GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG
   61 AAGCCTATAG AGTACGAGCC ATAGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG
  121 GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC
  181 AAGGCATGGA AAATACATAA CTGAGAATAG AGAAGTTCAG ATCAAGGTTA GGAACAGAGA
  241 GACAGCAGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG
  301 GCCAAGAACA GATGGTCCCC AGATGCGGTC CCGCCCTCAG CAGTTTCTAG AGAACCATCA
  361 GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA
  421 TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC
  481 ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATAGACTGC GTCGCCCGGG TACCCGTATT
  541 CCCAATAAAG CCTCTTGCTG TTTGCATCCG AATCGTGGAC TCGCTGATCC TTGGGAGGGT
  601 CTCCTCAGAT TGATTGACTG CCCACCTCGG GGGTCTTTCA TTTGGAGGTT CCACCGAGAT
  661 TTGGAGACCC CTGCCCAGGG ACCACCGACC CCCCCGCCGG GAGGTAAGCT GGCCAGCGGT
  721 CGTTTCGTGT CTGTCTCTGT CTTTGTGCGT GTTTGTGCCG GCATCTAATG TTTGCGCCTG
  781 CGTCTGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG AACTGACGAG
  841 TTCTGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTTGGGGG GCCGTTTTTG
  901 TGGCCCGACC TGAGGAAAGG GAGTCGATGT GGAATCCGAC CCCGTCAGGA TATGTGGGTT
  961 CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCCTCCG TCTGAATTTT TGCTTTCGGG
 1021 TTTGGAACCC GAAAGCCGCG CGTCTTGTCT GCTGCAGCGC TGCAGCATCG TTCTGTGATG
 1081 TCTCTGTCTG ACTGTGTTTC TGTAATTTGG TCTGAAATAA CGGACAGGAC TGATTAACCA
1141 CCTCCCTTAA GTATGGACCA TTAG
//
Primer: PGKpro_F (Pr0249)
Region: PGK pro, HygR 5'
Sequence file: RDB15408_A7Ikd.seq check
>D04516A8_A7Ik_1_PGKpro_F_E03_15_ABI24.ab1
    1 ACTGGGCGAT CTCTCTTCTC ATCTCCGGGC CTTTCGACCT GCAGCCCAAG CTTATGAAAA
   61 AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT
  121 CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG
  181 GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG
  241 TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAGT
  301 TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC
  361 TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG
  421 CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC
  481 AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC
  541 AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC
  601 TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA
  661 ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG
  721 GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG
  781 AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC
  841 GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT
  901 TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA
  961 CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGGCCG TCTGGGACCG ATGGGCTGTG
 1021 TAGAAGTACT CGCCCGATAG TGGAAACGGA CGCCCCAGCA CTCGTCCGAA GGCCAACGAT
 1081 AGATCGATAA AATAAAGATT TATTTAGTCT CAGAAAACGG GGGGAATGAA AGAACCCTCA
1141 ACC
//
Primer: HygR-C_F (Pr0501)
Region: HygR 3', 3'LTR
Sequence file: RDB15408_A7Ike.seq check
>D04516A8_A7Ik_1_HygR-C_F_H03_08_ABI08.ab1
    1 CGAAACGGGG GGATCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT
   61 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG
  121 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC
  181 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA
  241 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC
  301 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG
  361 GAATAGATCG ATAAAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA
  421 CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAT
  481 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTTAGGAA CAGAGAGACA GCAGAATATG
  541 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG
  601 GTCCCCAGAT GCGGTCCCGC CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT
  661 GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC
  721 GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC
  781 GGCGCGCCAG TCCTCCGATA GACTGCGTCG CCCGGGGTAC CCGTGTATCC AATAAACCCT
  841 CTTGCAGTTG CATCCGACTT GTGGTCTCGC TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT
  901 TGACTACCCG TCAGCGGGGG GTCTTTTCAT GGGGTAACAG TTTCTTGAAG TTTGGAGAAA
  961 CAACATTTCT GAGGGGTAGG GAGTCGAAAT ATTAAGTAAA TCCCTGACTC AATTTAGCCC
 1021 ACTGTTTTGA ATCGACATAC TCCCATACTC CCTTGAATTA GTTTCCATAT GGACAGCGCA
1081 GAAGAAGCCT GGGGAGAACT TGCTGCAAAT TGGTCATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Watanabe, Y., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]