Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMSCV-3xFLAG-mp62-del Zinc

Retroviral expression vector of mouse p62 deletion mutant (zinc finger domain, 123-167 a.a) tagged with 3xFLAG at N-terminal.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15406_A7Ikp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: gag-M_F Region: gag3', 3xFLAG, insert5' Sequence file: RDB15406_A7Ika.seq
>D04516A6_A7Ik_2_gag-M_F_C01_07_ABI24.ab1     1 ATGTGGTAGC CTTTGTAACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG CCCCGTCTCT    61 CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG CCCTCACTCC   121 TTCTCTAGGC GCCGGAATTA GATCCATGGA TTACAAGGAT GACGACGATA AGAGATCCGA   181 TTACAAGGAT GACGACGATA AGAGATCCGA TTACAAGGAT GACGACGATA AGAGATCCCC   241 CGGGCTGCAG GAATTCATGG CGTCGTTCAC GGTGAAGGCC TATCTTCTGG GCAAGGAGGA   301 GGCGACCCGC GAGATCCGCC GCTTCAGCTT CTGCTTCAGC CCGGAGCCGG AGGCGGAAGC   361 CCAAGCCGCG GCCGGCCCGG GGCCCTGCGA GAGGCTGCTG AGCCGAGTGG CTGTGCTGTT   421 CCCCACGCTG AGGCCTGGCG GCTTCCAGGC GCACTACCGC GATGAGGATG GGGACTTGGT   481 TGCCTTTTCC AGTGATGAGG AGCTGACAAT GGCTATGTCC TATGTGAAAG ATGACATCTT   541 CCGCATCTAC ATTAAAGAGA AGAAGGAGTG CCGGCGGGAA CATCGCCCAC CATGTGCTCA   601 GGAGGCACCC CGAAACATGG TGGGATCTTT TCCCAACCCC TTTGGCCACC TCTCTGATAG   661 CTTCTCTCAT AGCCGCTGGC TTCGGAAGCT GAAACATGGA CACTTTGGCT GGCCTGGCTG   721 GGAGATGGGC CCACCGGGGA ACTGGAGCCC ACGTCCTCCT CGTGCAGGGG ATGGCCGCCC   781 TTGCCCTACA GCTGAGTCAG CTTCTGCTCC ACCAGAAGAT CCCAATGTCA ATTTCCTGAA   841 GAATGTGGGG GAGAGTGTGG CAGCTGCCCT CAGCCCTCTA GGCATTGAGG TTGACATTGA   901 TGTGGAACAT GGAGGGAAGA GAAGCCGCCT GACACCCACT ACCCCAGAAA GTTCCAGCAC   961 AGGCACAGAA GACAAGAGTA ACACTCAGCC AAGCAGCTGC TCTTCGGGAA GTCAGCAAAC  1021 CCTGACGGGG CTGGGGGAGG CCCCTGCTCA GTCTCTGACA GAGCAAATGA AAAAGATAGC 1081 CCTTGGTGGT CCGTGGACAG CAGAGCACAG ATGGAAGTCG GCAAAACTGC CCTTCAG //
Primer: PGK_N_rev Region: insert3' Sequence file: RDB15406_A7Ikb.seq
>D04516A6_A7Ik_2_PGK_N_rev_D01_10_ABI24.ab1     1 CCTTTAACGC CTCCCTACCG GTAGATTCGT TAACCTCGAG TCACAATGGT GGAGGGTGCT    61 TCGAATACTG GATCGTGTCC AGAGCAGCCC CGATGTCGTA ATTCTTGGTC TGTAGGAGCC   121 TGGTGAGCCA GCCGCCTTCA TCCGAGAAAC CCATGGACAG CATCTGGGAG AGGGACTCAA   181 TCAGCCGGGG ATCAGCCTCT GGTGGGAGAT GTGGGTATAG GGCAGCTTCC TTCAGCCCTG   241 TGGGTCCTTC CTGTGAGGGG TCTAGAGAGC TTGGCCCTTC CGATTCTGGC ATCTGTAGAG   301 ACTGGAGTTC ACCTGTAGAT GGGTCCACTT CTTTTGAAGA CAAATGTGTC CAGTCATCGT   361 CTCCTCCTGA GCAGTTTCCC GACTCCATCT GTTCCTCTGG CTGTCCCACC GACTCCAAGG   421 CTATCTTTTT CATTTGCTCT GTCAGAGACT GAGCAGGGCC CTCCCCAGCC CCGTCAGGTT   481 TGCTGACTTC CGAAGAGCAG CTGCTTGGCT GAGTGTTACT CTTGTCTTCT GTGCCTGTGC   541 TGGAACTTTC TGGGGTAGTG GGTGTCAGGC GGCTTCTCTT CCCTCCATGT TCCACATCAA   601 TGTCAACCTC AATGCCTAGA GGGCTGAGGG CAGCTGCCAC ACTCTCCCCC ACATTCTTCA   661 GGAAATTGAC ATTGGGATCT TCTGGTGGAG CAGAAGCTGA CTCAGCTGTA GGGCAAGGGC   721 GGCCATCCCC TGCACGAGGA GGACGTGGGC TCCAGTTCCC CGGTGGGCCC ATCTCCCAGC   781 CAGGCCAGCC AAAGTGTCCA TGTTTCAGCT TCCGAAGCCA GCGGCTATGA GAGAAGCTAT   841 CAGAGAGGTG GCCAAAGGGG TTGGGAAAAG ATCCCACCAT GTTTCGGGGT GCCTCCTGAG   901 CACATGGTGG GCGATGTTCC CGCCGGCACT CCTTCTTCTC TTTAATGTAG ATGCGGAAGA   961 TGTCATCTTT CACATAGGAC ATAGCCATTG TCAGCTCCTC ATCACTGGAA AAGGCAACCC  1021 AAGTCCCCAT CCCTCATCGC GGTAGTGCGC CCTGGAAAGC CGCCAGGGCC CTCAGCGTGG  1081 GGAACAGCAC AGCCCACTTC GACTCAGCAG CTCTCGCAGG ACCCCGGAAC CGGACCGCCG 1141 GACTTGGGGA CCCTC //
Primer: pBR322_BamHI_R Region: 5'LTR, MESV psi Sequence file: RDB15406_A7Ikc.seq
>D04516A6_A7Ik_2_pBR322_BamHI_R_E01_13_ABI24.ab1     1 ATATTAAGAA GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG    61 AAGCCTATAG AGTACGAGCC ATAGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG   121 GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC   181 AAGGCATGGA AAATACATAA CTGAGAATAG AGAAGTTCAG ATCAAGGTTA GGAACAGAGA   241 GACAGCAGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG   301 GCCAAGAACA GATGGTCCCC AGATGCGGTC CCGCCCTCAG CAGTTTCTAG AGAACCATCA   361 GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA   421 TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC   481 ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATAGACTGC GTCGCCCGGG TACCCGTATT   541 CCCAATAAAG CCTCTTGCTG TTTGCATCCG AATCGTGGAC TCGCTGATCC TTGGGAGGGT   601 CTCCTCAGAT TGATTGACTG CCCACCTCGG GGGTCTTTCA TTTGGAGGTT CCACCGAGAT   661 TTGGAGACCC CTGCCCAGGG ACCACCGACC CCCCCGCCGG GAGGTAAGCT GGCCAGCGGT   721 CGTTTCGTGT CTGTCTCTGT CTTTGTGCGT GTTTGTGCCG GCATCTAATG TTTGCGCCTG   781 CGTCTGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG AACTGACGAG   841 TTCTGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTTGGGGG CCGTTTTTGT   901 GGCCCGACCT GAGGAAGGGA GTCGATGTGG AATCCGACCC CGTCAGGATA TGTGGTTCTG   961 GTAGGAGACG AGAACCTAAA ACAGTTCCCG CCCTCCGTCT GAATTTTTGC TTTCGGGTTT  1021 GGAACCGAAG CCGCGCGTCT TGTCTGCTGC AGCGGCTGCA GCATCGATCT GTGTGGTCTC  1081 TGTCTGACTG TGTTCTTGTA TGACTGACAC TTACGACAGA CCTGATTAAC CACCTCCCAT 1141 AAGATTTGGA CCGTG //
Primer: PGKpro_F Region: PGK pro, HygR 5' Sequence file: RDB15406_A7Ikd.seq
>D04516A6_A7Ik_2_PGKpro_F_G01_19_ABI24.ab1     1 AGGGGGCGTA TCTCTCTTCT CATCTCCGGG CCTTTCGACC TGCAGCCCAA GCTTATGAAA    61 AAGCCTGAAC TCACCGCGAC GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC   121 TCCGACCTGA TGCAGCTCTC GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA   181 GGGCGTGGAT ATGTCCTGCG GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT   241 GTTTATCGGC ACTTTGCATC GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAG   301 TTCAGCGAGA GCCTGACCTA TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC   361 CTGCCTGAAA CCGAACTGCC CGCTGTTCTG CAGCCGGTCG CGGAGGCCAT GGATGCGATC   421 GCTGCGGCCG ATCTTAGCCA GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT   481 CAATACACTA CATGGCGTGA TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG   541 CAAACTGTGA TGGACGACAC CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG   601 CTTTGGGCCG AGGACTGCCC CGAAGTCCGG CACCTCGTGC ACGCGGATTT CGGCTCCAAC   661 AATGTCCTGA CGGACAATGG CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC   721 GGGGATTCCC AATACGAGGT CGCCAACATC TTCTTCTGGA GGCCGTGGTT GGCTTGTATG   781 GAGCAGCAGA CGCGCTACTT CGAGCGGAGG CATCCGGAGC TTGCAGGATC GCCGCGGCTC   841 CGGGCGTATA TGCTCCGCAT TGGTCTTGAC CAACTCTATC AGAGCTTGGT TGACGGCAAT   901 TTCGATGATG CAGCTTGGGC GCAGGGTCGA TGCGACGCAA TCGTCCGATC CGGAGCCGGG   961 ACTGTCGGGC GTACACAAAT CGCCCGCAGA AGCGCGGCCG TCTGGGACCG ATGGGCTGTG  1021 TAGAAGTACT CGCCGATAGT GGAACGACGC CCCAGCACTC GTCCGAGGCC AAAGATTAGA 1081 TCGATAAATA ATGATTTATT AGGTCTCAGG AAAGGGGGGA TGAAAGACCC CCACCCTG //
Primer: HygR-C_F Region: HygR 3', 3'LTR Sequence file: RDB15406_A7Ike.seq
>D04516A6_A7Ik_2_HygR-C_F_G02_07_ABI08.ab1     1 CGTTAGGGGG GATCCAATAC GAGGTCGCCA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG    61 TATGGAGCAG CAGACGCGCT ACTTCGAGCG GAGGCATCCG GAGCTTGCAG GATCGCCGCG   121 GCTCCGGGCG TATATGCTCC GCATTGGTCT TGACCAACTC TATCAGAGCT TGGTTGACGG   181 CAATTTCGAT GATGCAGCTT GGGCGCAGGG TCGATGCGAC GCAATCGTCC GATCCGGAGC   241 CGGGACTGTC GGGCGTACAC AAATCGCCCG CAGAAGCGCG GCCGTCTGGA CCGATGGCTG   301 TGTAGAAGTA CTCGCCGATA GTGGAAACCG ACGCCCCAGC ACTCGTCCGA GGGCAAAGGA   361 ATAGATCGAT AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC   421 CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAATAC   481 ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTTAGGAACA GAGAGACAGC AGAATATGGG   541 CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT   601 CCCCAGATGC GGTCCCGCCC TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC   661 CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC   721 TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG   781 CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT   841 GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGGA GGGTCTCCTC TGAGTGATTG   901 ACTACCCGTC AGCGGGGGGT CTTTCATGGG TAACAGTTTT CTTGAAGTTG GGAGAACAAC   961 ATTCTGAGGG GTAGGAGTCG GAATATTAAG TAATCCCTGA CTCATTAGCC CACTGGTTTT  1021 GAATCCACAT ACTCCCAATA CTCCTGAATA GTCCAATAAT GGGACAGCGC AGATGAGCCT 1081 TGGGCAGCAC TTGCTGCAAA TTTGGGCTTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl