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pMSCV-3xFLAG-mp62-del Zinc (#RDB15406)

Retroviral expression vector of mouse p62 deletion mutant (zinc finger domain, 123-167 a.a) tagged with 3xFLAG at N-terminal.

Clone info. Retroviral expression vector of mouse p62 deletion mutant (zinc finger domain, 123-167 a.a) tagged with 3xFLAG at N-terminal. See Fig.5e of Watanabe, Y. et al. Nat. Commun., 7: 13508, 2016.
Comment Expression was confirmed by the depositor with immunostaining and western blotting.
Vector backbone pMSCV–Flag(3x) (plasmid)
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells).
Gene/insert name mouse p62 cDNA
Depositor|Developer Shimizu, Shigeomi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15406 pMSCV-3xFLAG-mp62-del Zinc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMSCV-3xFLAG-mp62-del Zinc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15406).

Reference section:

Watanabe, Y., Honda, S., Konishi, A., Arakawa, S., Murohashi, M., Yamaguchi, H., Torii, S., Tanabe, M., Tanaka, S., Warabi, E., Shimizu, S., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15406_A7Ikp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: gag-M_F (Pr0730)
Region: gag3', 3xFLAG, insert5'
Sequence file: RDB15406_A7Ika.seq check
>D04516A6_A7Ik_2_gag-M_F_C01_07_ABI24.ab1
    1 ATGTGGTAGC CTTTGTAACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG CCCCGTCTCT
   61 CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG CCCTCACTCC
  121 TTCTCTAGGC GCCGGAATTA GATCCATGGA TTACAAGGAT GACGACGATA AGAGATCCGA
  181 TTACAAGGAT GACGACGATA AGAGATCCGA TTACAAGGAT GACGACGATA AGAGATCCCC
  241 CGGGCTGCAG GAATTCATGG CGTCGTTCAC GGTGAAGGCC TATCTTCTGG GCAAGGAGGA
  301 GGCGACCCGC GAGATCCGCC GCTTCAGCTT CTGCTTCAGC CCGGAGCCGG AGGCGGAAGC
  361 CCAAGCCGCG GCCGGCCCGG GGCCCTGCGA GAGGCTGCTG AGCCGAGTGG CTGTGCTGTT
  421 CCCCACGCTG AGGCCTGGCG GCTTCCAGGC GCACTACCGC GATGAGGATG GGGACTTGGT
  481 TGCCTTTTCC AGTGATGAGG AGCTGACAAT GGCTATGTCC TATGTGAAAG ATGACATCTT
  541 CCGCATCTAC ATTAAAGAGA AGAAGGAGTG CCGGCGGGAA CATCGCCCAC CATGTGCTCA
  601 GGAGGCACCC CGAAACATGG TGGGATCTTT TCCCAACCCC TTTGGCCACC TCTCTGATAG
  661 CTTCTCTCAT AGCCGCTGGC TTCGGAAGCT GAAACATGGA CACTTTGGCT GGCCTGGCTG
  721 GGAGATGGGC CCACCGGGGA ACTGGAGCCC ACGTCCTCCT CGTGCAGGGG ATGGCCGCCC
  781 TTGCCCTACA GCTGAGTCAG CTTCTGCTCC ACCAGAAGAT CCCAATGTCA ATTTCCTGAA
  841 GAATGTGGGG GAGAGTGTGG CAGCTGCCCT CAGCCCTCTA GGCATTGAGG TTGACATTGA
  901 TGTGGAACAT GGAGGGAAGA GAAGCCGCCT GACACCCACT ACCCCAGAAA GTTCCAGCAC
  961 AGGCACAGAA GACAAGAGTA ACACTCAGCC AAGCAGCTGC TCTTCGGGAA GTCAGCAAAC
 1021 CCTGACGGGG CTGGGGGAGG CCCCTGCTCA GTCTCTGACA GAGCAAATGA AAAAGATAGC
1081 CCTTGGTGGT CCGTGGACAG CAGAGCACAG ATGGAAGTCG GCAAAACTGC CCTTCAG
//
Primer: PGK_N_rev (Pr0243)
Region: insert3'
Sequence file: RDB15406_A7Ikb.seq check
>D04516A6_A7Ik_2_PGK_N_rev_D01_10_ABI24.ab1
    1 CCTTTAACGC CTCCCTACCG GTAGATTCGT TAACCTCGAG TCACAATGGT GGAGGGTGCT
   61 TCGAATACTG GATCGTGTCC AGAGCAGCCC CGATGTCGTA ATTCTTGGTC TGTAGGAGCC
  121 TGGTGAGCCA GCCGCCTTCA TCCGAGAAAC CCATGGACAG CATCTGGGAG AGGGACTCAA
  181 TCAGCCGGGG ATCAGCCTCT GGTGGGAGAT GTGGGTATAG GGCAGCTTCC TTCAGCCCTG
  241 TGGGTCCTTC CTGTGAGGGG TCTAGAGAGC TTGGCCCTTC CGATTCTGGC ATCTGTAGAG
  301 ACTGGAGTTC ACCTGTAGAT GGGTCCACTT CTTTTGAAGA CAAATGTGTC CAGTCATCGT
  361 CTCCTCCTGA GCAGTTTCCC GACTCCATCT GTTCCTCTGG CTGTCCCACC GACTCCAAGG
  421 CTATCTTTTT CATTTGCTCT GTCAGAGACT GAGCAGGGCC CTCCCCAGCC CCGTCAGGTT
  481 TGCTGACTTC CGAAGAGCAG CTGCTTGGCT GAGTGTTACT CTTGTCTTCT GTGCCTGTGC
  541 TGGAACTTTC TGGGGTAGTG GGTGTCAGGC GGCTTCTCTT CCCTCCATGT TCCACATCAA
  601 TGTCAACCTC AATGCCTAGA GGGCTGAGGG CAGCTGCCAC ACTCTCCCCC ACATTCTTCA
  661 GGAAATTGAC ATTGGGATCT TCTGGTGGAG CAGAAGCTGA CTCAGCTGTA GGGCAAGGGC
  721 GGCCATCCCC TGCACGAGGA GGACGTGGGC TCCAGTTCCC CGGTGGGCCC ATCTCCCAGC
  781 CAGGCCAGCC AAAGTGTCCA TGTTTCAGCT TCCGAAGCCA GCGGCTATGA GAGAAGCTAT
  841 CAGAGAGGTG GCCAAAGGGG TTGGGAAAAG ATCCCACCAT GTTTCGGGGT GCCTCCTGAG
  901 CACATGGTGG GCGATGTTCC CGCCGGCACT CCTTCTTCTC TTTAATGTAG ATGCGGAAGA
  961 TGTCATCTTT CACATAGGAC ATAGCCATTG TCAGCTCCTC ATCACTGGAA AAGGCAACCC
 1021 AAGTCCCCAT CCCTCATCGC GGTAGTGCGC CCTGGAAAGC CGCCAGGGCC CTCAGCGTGG
 1081 GGAACAGCAC AGCCCACTTC GACTCAGCAG CTCTCGCAGG ACCCCGGAAC CGGACCGCCG
1141 GACTTGGGGA CCCTC
//
Primer: pBR322_BamHI_R (Pr0065)
Region: 5'LTR, MESV psi
Sequence file: RDB15406_A7Ikc.seq check
>D04516A6_A7Ik_2_pBR322_BamHI_R_E01_13_ABI24.ab1
    1 ATATTAAGAA GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG
   61 AAGCCTATAG AGTACGAGCC ATAGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG
  121 GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC
  181 AAGGCATGGA AAATACATAA CTGAGAATAG AGAAGTTCAG ATCAAGGTTA GGAACAGAGA
  241 GACAGCAGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG
  301 GCCAAGAACA GATGGTCCCC AGATGCGGTC CCGCCCTCAG CAGTTTCTAG AGAACCATCA
  361 GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA
  421 TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC
  481 ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATAGACTGC GTCGCCCGGG TACCCGTATT
  541 CCCAATAAAG CCTCTTGCTG TTTGCATCCG AATCGTGGAC TCGCTGATCC TTGGGAGGGT
  601 CTCCTCAGAT TGATTGACTG CCCACCTCGG GGGTCTTTCA TTTGGAGGTT CCACCGAGAT
  661 TTGGAGACCC CTGCCCAGGG ACCACCGACC CCCCCGCCGG GAGGTAAGCT GGCCAGCGGT
  721 CGTTTCGTGT CTGTCTCTGT CTTTGTGCGT GTTTGTGCCG GCATCTAATG TTTGCGCCTG
  781 CGTCTGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG AACTGACGAG
  841 TTCTGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTTGGGGG CCGTTTTTGT
  901 GGCCCGACCT GAGGAAGGGA GTCGATGTGG AATCCGACCC CGTCAGGATA TGTGGTTCTG
  961 GTAGGAGACG AGAACCTAAA ACAGTTCCCG CCCTCCGTCT GAATTTTTGC TTTCGGGTTT
 1021 GGAACCGAAG CCGCGCGTCT TGTCTGCTGC AGCGGCTGCA GCATCGATCT GTGTGGTCTC
 1081 TGTCTGACTG TGTTCTTGTA TGACTGACAC TTACGACAGA CCTGATTAAC CACCTCCCAT
1141 AAGATTTGGA CCGTG
//
Primer: PGKpro_F (Pr0249)
Region: PGK pro, HygR 5'
Sequence file: RDB15406_A7Ikd.seq check
>D04516A6_A7Ik_2_PGKpro_F_G01_19_ABI24.ab1
    1 AGGGGGCGTA TCTCTCTTCT CATCTCCGGG CCTTTCGACC TGCAGCCCAA GCTTATGAAA
   61 AAGCCTGAAC TCACCGCGAC GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC
  121 TCCGACCTGA TGCAGCTCTC GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA
  181 GGGCGTGGAT ATGTCCTGCG GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT
  241 GTTTATCGGC ACTTTGCATC GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAG
  301 TTCAGCGAGA GCCTGACCTA TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC
  361 CTGCCTGAAA CCGAACTGCC CGCTGTTCTG CAGCCGGTCG CGGAGGCCAT GGATGCGATC
  421 GCTGCGGCCG ATCTTAGCCA GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT
  481 CAATACACTA CATGGCGTGA TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG
  541 CAAACTGTGA TGGACGACAC CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG
  601 CTTTGGGCCG AGGACTGCCC CGAAGTCCGG CACCTCGTGC ACGCGGATTT CGGCTCCAAC
  661 AATGTCCTGA CGGACAATGG CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC
  721 GGGGATTCCC AATACGAGGT CGCCAACATC TTCTTCTGGA GGCCGTGGTT GGCTTGTATG
  781 GAGCAGCAGA CGCGCTACTT CGAGCGGAGG CATCCGGAGC TTGCAGGATC GCCGCGGCTC
  841 CGGGCGTATA TGCTCCGCAT TGGTCTTGAC CAACTCTATC AGAGCTTGGT TGACGGCAAT
  901 TTCGATGATG CAGCTTGGGC GCAGGGTCGA TGCGACGCAA TCGTCCGATC CGGAGCCGGG
  961 ACTGTCGGGC GTACACAAAT CGCCCGCAGA AGCGCGGCCG TCTGGGACCG ATGGGCTGTG
 1021 TAGAAGTACT CGCCGATAGT GGAACGACGC CCCAGCACTC GTCCGAGGCC AAAGATTAGA
1081 TCGATAAATA ATGATTTATT AGGTCTCAGG AAAGGGGGGA TGAAAGACCC CCACCCTG
//
Primer: HygR-C_F (Pr0501)
Region: HygR 3', 3'LTR
Sequence file: RDB15406_A7Ike.seq check
>D04516A6_A7Ik_2_HygR-C_F_G02_07_ABI08.ab1
    1 CGTTAGGGGG GATCCAATAC GAGGTCGCCA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG
   61 TATGGAGCAG CAGACGCGCT ACTTCGAGCG GAGGCATCCG GAGCTTGCAG GATCGCCGCG
  121 GCTCCGGGCG TATATGCTCC GCATTGGTCT TGACCAACTC TATCAGAGCT TGGTTGACGG
  181 CAATTTCGAT GATGCAGCTT GGGCGCAGGG TCGATGCGAC GCAATCGTCC GATCCGGAGC
  241 CGGGACTGTC GGGCGTACAC AAATCGCCCG CAGAAGCGCG GCCGTCTGGA CCGATGGCTG
  301 TGTAGAAGTA CTCGCCGATA GTGGAAACCG ACGCCCCAGC ACTCGTCCGA GGGCAAAGGA
  361 ATAGATCGAT AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC
  421 CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAATAC
  481 ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTTAGGAACA GAGAGACAGC AGAATATGGG
  541 CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT
  601 CCCCAGATGC GGTCCCGCCC TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC
  661 CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC
  721 TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG
  781 CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT
  841 GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGGA GGGTCTCCTC TGAGTGATTG
  901 ACTACCCGTC AGCGGGGGGT CTTTCATGGG TAACAGTTTT CTTGAAGTTG GGAGAACAAC
  961 ATTCTGAGGG GTAGGAGTCG GAATATTAAG TAATCCCTGA CTCATTAGCC CACTGGTTTT
 1021 GAATCCACAT ACTCCCAATA CTCCTGAATA GTCCAATAAT GGGACAGCGC AGATGAGCCT
1081 TGGGCAGCAC TTGCTGCAAA TTTGGGCTTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Watanabe, Y., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]