Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMSCV-GFP-hCep63

Retroviral expression vector of human Cep63 fused with EGFP at N-terminal.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15403_A7Ikp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: gag_t-C_F (Pr0730) Region: gag 3', EGFP, insert 5' Sequence file: RDB15403_A7Ika.seq
>D04516A3_A7Ik_1_gag_t-C_F_C02_03_ABI08.ab1     1 ACCGGGAAAC TCCTTTATCC AGCCCTCACT CCTTCTCTAG GCGCCGGAAT TAGATCCATG    61 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC   121 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC   181 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC   241 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG   301 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC   361 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG   421 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG   481 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC   541 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC   601 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC   661 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG   721 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGAGATCC   781 ATGGAGGCTT TGTTAGAAGG AATACAAAAT CGAGGGCATG GTGGGGGATT TTTGACATCT   841 TGTGAAGCAG AACTACAGGA GCTCATGAAA CAGATTGACA TAAATGGTGG CTCATAAAAA   901 ATCTGAATGG GGAAGGACGT ACACATGCTC TAGAAACTTT GCTTGAAAAT CCGTGAACAG   961 GAACTTAAAG AGTCTTAGGA GTCAGTTGGA TGTGACACAT AAAGGAAGGT TGGAAATGTT  1021 GCATCAGCAG GTAGAAGGAA CATGAAAAAA TCAAGCAAGA GATGACCATT GGAATATAGC 1081 AAGCAGTTTG AAAGAAACCT ACATGTGAAG AGAT //
Primer: EGFP-C (Pr0025) Region: EGFP3', insert5' Sequence file: RDB15403_A7Ikb.seq
>D04516A3_A7Ik_1_EGFP_C_C05_08_ABI24.ab1     1 CAGCGGGAAA CCATTCTCGG GGCATGGGGA CGAGCTGTAC AAGAGATCCA TGGAGGCTTT    61 GTTAGAAGGA ATACAAAATC GAGGGCATGG TGGGGGATTT TTGACATCTT GTGAAGCAGA   121 ACTACAGGAG CTCATGAAAC AGATTGACAT AATGGTGGCT CATAAAAAAT CTGAATGGGA   181 AGGACGTACA CATGCTCTAG AAACTTGCTT GAAAATCCGT GAACAGGAAC TTAAGAGTCT   241 TAGGAGTCAG TTGGATGTGA CACATAAGGA GGTTGGAATG TTGCATCAGC AGGTAGAAGA   301 ACATGAAAAA ATCAAGCAAG AGATGACCAT GGAATATAAG CAGGAGTTGA AGAAACTACA   361 TGAAGAATTA TGCATACTGA AGAGAAGCTA TGAAAAGCTT CAGAAAAAGC AAATGAGGGA   421 ATTCAGAGGA AATACCAAAA ATCACAGGGA AGATCGGTCT GAAATTGAGA GGTTAACTGC   481 AAAAATAGAG GAATTCCGTC AGAAATCGCT GGACTGGGAG AAGCAACGCT TGATTTATCA   541 GCAACAGGTA TCTTCACTGG AGGCACAAAG GAAGGCTCTG GCTGAACAAT CAGAGATAAT   601 TCAGGCTCAG CTTGTCAATC GGAAACAGAA ATTAGAGTCT GTGGAACTTT CTAGCCAATC   661 AGAAATTCAA CACTTAAGCA GTAAACTGGA GCGGGCTAAT GACACTATCT GTGCCAATGA   721 GTTGGAAATA GAGCGCCTCA CCATGAGGGT CAATGACTTG GTTGGAACCA GTATGACTGT   781 CCTACAGGAG CAGCAGCAAA AAGAAGAAAA ATTGAGGGAA TCTGAAAAAC TATTAGAGGC   841 TCTGCAGGAA GAAAAGAGAG AATTGAAGGC AGCTCTTCAG TCTCAAGAAA ATCTCATACA   901 TGAGGCCAGA ATACAAAAGG AGAAGTTACA AGGAAAAAGT AAAAGGCAAC TAACACTCAA   961 CATGCTGTAG AAGCTATAAA GGCCACGGGA AGAATCTCTG GCAGAAAAGA AGTACCACCT  1021 TCTCAGGGCA GGGGACTTTA GACAGTGTGC CTCCTCCCCA GTTGAATTTT ACCCATACTA  1081 GTGAGGACCT TTCTGGCAGC AGAGTGACTT GTTCTTGAGC CAGTTGATCT TGTGGATGCC 1141 AACGGTGTTA AACCAGCCCT GGAGGCCCAC AGA //
Primer: PGK_N_rev (Pr0243) Region: insert3' Sequence file: RDB15403_A7Ikc.seq
>D04516A3_A7Ik_1_PGK_N_rev_G05_20_ABI24.ab1     1 CATTGGACGC TCCCTACCCG GTAGATTCGT TAACCTCGAG CTACTTTAAG GCTGTGAATT    61 GTCTCACTGT CTTTTCACTC TCTCTTTTTA GTTCTTCAAT ATGGGCATCC AAGCGCTCTA   121 GAATGTGATG AGACCTCAGT TCCTCCTCTT CCAAAAATCT GGTAGCTATT GAACCAAGGG   181 GAGATACAGG CAAGGAACAC GAAGATATAA ACTCTTCTTG GTCATTCATA CTCTCAGACA   241 TAGTGTCAGA AAAATTGGCT TCATTTGTAT CTAGCGCTGA AGGCAAAGAA TGAGTTTTAC   301 ATAGAGAGTA GGAAGTCAAA GACCTGGTGG AGCTGCAAGG GCTGATTTGA GGACTCAGGG   361 GGCTTAGCAC CCTAGACATG GGGTTTATAC TATCCGACGC TTGTCCTCTT GGAGAATGAA   421 GATCTGTTTT GTAGTGCTCA GTCTTTATTC CATCATGTCT GTGCTGGCCA TGGGTTGGCT   481 TGAACTCTGT ATTCTTGAAC TCAGTTGTTC TGCTGTGTGT TGGTTTAAAG ATCCTGTCAT   541 TTTGTTTTTT GCACATCTGA GTAGAAATCT CCAGCTGAGA TTTGGTATTC ATCAAATCTT   601 TCTGTAGTTG TTGATTTTCA GACACTAATT TATTTAATGC CTCAATATAA TTCTCCTGGA   661 GGTCTGCTAG TGAAATCTCT TTTGCCTCAT GTAAACCCAA TTCCAATTTC ATCACCATTT   721 CAGAAAGATG ACGATTTTCT AATTTGAGTG ACTCCAGCTG ATCCAAAATC TCCTTATGCT   781 CTACTGCTTT GTCTTCTGCC TTTTGCATCT CTGCTCTGAG TCGCTTTTCC ATGTCTGAGG   841 AAGAACCTTT GGTGGAAGCA ATAGTGATAT CTCGCTGATG TAACTCCTGA GTTAGATGGG   901 AGATTTCCAT CTTCATTCCT TCTAGTGCAG AACTGTAACT TTGTTCACCC TGTAAAATCT   961 GTTCTTTCAA CTTCTTAATC TCTGCTTTGT ATTCATTGTT ATGTGCTTTC CATCCCTCTT  1021 CAGTTCTTTC ATATTTTTCC ATTTAGACTT GGCTCAGCTG TTTTACACGT GGCACTCACA  1081 GATTTCCCAA ACTGTCTCAG ACCAGTTCAC CTCTGGCCTG CAGAAGGATC CTCCACTAGG 1141 TAATTGCGT //
Primer: pBR322_BamHI_R (Pr0065) Region: 5'LTR, MESV psi Sequence file: RDB15403_A7Ikd.seq
>D04516A3_A7Ik_1_pBR322_BamHI_R_E05_14_ABI24.ab1     1 TGTTTAAGGA AGGATATCAG TGGTCCAGGC TCTAGTTTTG ACTCAACAAT ATCACCAGCT    61 GAAGCCTATA GAGTACGAGC CATAGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA   121 GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG   181 CAAGGCATGG AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTT AGGAACAGAG   241 AGACAGCAGA ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG   301 GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCCGCCCTCA GCAGTTTCTA GAGAACCATC   361 AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA   421 ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC   481 CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATAGACTG CGTCGCCCGG GTACCCGTAT   541 TCCCAATAAA GCCTCTTGCT GTTTGCATCC GAATCGTGGA CTCGCTGATC CTTGGGAGGG   601 TCTCCTCAGA TTGATTGACT GCCCACCTCG GGGGTCTTTC ATTTGGAGGT TCCACCGAGA   661 TTTGGAGACC CCTGCCCAGG GACCACCGAC CCCCCCGCCG GGAGGTAAGC TGGCCAGCGG   721 TCGTTTCGTG TCTGTCTCTG TCTTTGTGCG TGTTTGTGCC GGCATCTAAT GTTTGCGCCT   781 GCGTCTGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG GAACTGACGA   841 GTTCTGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTTGGGG GCCGTTTTTG   901 TGGCCCGACC TGAGGAAGGG AGTCGATGTG GAATCCGACC CCGTCAGGAT ATGTGGTTCT   961 GGTAGGAGAC GAGAACCTAA AACAGTTTCC CGCCTCCGTC TGAAATTTTT TGCTTTCGGG  1021 TTTGGAACCC GAAAGCCCGC GCGGTCTTGT CTGCTGCAGC GCTGCAGCAT CGATTCTGTG  1081 ATGTCTCTGT CTGACTGTGT TTCCTGTATT TGGTCCTGAA AATTAGATCA GACTGATTAC 1141 CCACTCCCCT TTTAAGGGGT T //
Primer: EGFP-N (Pr0027) Region: EGFP, gag, MESV psi, 5' LTR Sequence file: RDB15403_A7Ike.seq
>D04516A3_A7Ik_1_EGFP_N_D05_11_ABI24.ab1     1 GTAAACCGGG GGGGGACAGC TCCTCGCCCT TGCTCACCAT GGATCTAATT CCGGCGCCTA    61 GAGAAGGAGT GAGGGCTGGA TAAAGGGAGG ATCGAGGCGG GGTCGAACGA GGAGGTTCAA   121 GGGGGAGAGA CGGGGCGGAT GGAGGAAGAG GAGGCGGAGG CTTAGGGTGT ACAAAGGGCT   181 TGACCCAGGG AGGGGGGTCA AAAGCCAAGG CTTCCCAGGT CACGATGTAG GGGACCTGGT   241 CTGGGTGTCC ATGCGGGCCA GGTGAAAAGA CCTTGATCTT AACCTGGGTG ATGAGGTCTC   301 GGTTAAAGGT GCCGTCTCGC GGCCATCCGA CGTTAAAGGT TGGCCATTCT GCAGAGCAGA   361 AGGTAACCCA ACGTCTCTTC TTGACATCTA CCGACTGGTT GTGAGCGATC CGCTCGACAT   421 CTTTCCAGTG ACCTAAGGTC AAACTTAAGG GAGTGGTAAC AGTCTGGCCC TAATTTTCAG   481 ACAAATACAG AAACACAGTC AGACAGAGAC AACACAGAAC GATGCTGCAG CGCTGCAGCA   541 GACAAGACGC GCGGCTTCGG TTCCAAACCG AAAGCAAAAA TTCAGACGGA GGCGGGAACT   601 GTTTTAGGTT CTCGTCTCCT ACCAGAACCA CATATCCTGA CGGGGTCGGA TTCCACATCG   661 ACTCCCTTCC TCAGGTCGGG CCACAAAAAC GGCCCCCAAA GTCCCTGGGA CGTCTCCCAG   721 GGTTGCGGCC GGGTGTTCAG AACTCGTCAG TTCCACCACG GGTCCGCCAG ATACAGAGCT   781 AGTTAGCTAA CTAGTACAGA CGCAGGCGCA AACATTAGAT GCCGGCACAA ACACGCACAA   841 AGACAGAGAC AGACACGAAA CGACCGCTGG CCAGCTTACC TCCCGGCGGG GGGGTCGGTG   901 GTCCCTGGGC AGGGGTCTCC AAATCTCGGT GGAACCTCCA AATGAAAGAC CCCCGAGGTG   961 GCAGTCAATC AATCTGAGGA GACCCTCCCA AGGATCAGCG AGTCCACGAT TCGATGCAAA  1021 ACAGCAAGAG CTTTTATTGG AATACGGTAC CCGGCGACGC AGTCTATCGG AGACTGCGCG  1081 TGAGTGAGGA TTGTGGCTCT TTAATGACTC GGGAGCAGAA GCGGCGCGAA CCAGAAGTCG 1141 C //
Primer: PGKpro_F (Pr0249) Region: PGK pro, HygR, 3'LTR Sequence file: RDB15403_A7Ikf.seq
>D04516A3_A7Ik_1_PGKpro_F_A07_01_ABI24.ab1     1 AGGGGGGCCG TATCTCTCTT CTCATCTCCG GGCCTTTCGA CCTGCAGCCC AAGCTTATGA    61 AAAAGCCTGA ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG   121 TCTCCGACCT GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG   181 GAGGGCGTGG ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT   241 ATGTTTATCG GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG   301 AGTTCAGCGA GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG   361 ACCTGCCTGA AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA   421 TCGCTGCGGC CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG   481 GTCAATACAC TACATGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT   541 GGCAAACTGT GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA   601 TGCTTTGGGC CGAGGACTGC CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA   661 ACAATGTCCT GACGGACAAT GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT   721 TCGGGGATTC CCAATACGAG GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA   781 TGGAGCAGCA GACGCGCTAC TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCGCGGC   841 TCCGGGCGTA TATGCTCCGC ATTGGTCTTG ACCAACTCTA TCAGAGCTTG GTTGACGGCA   901 ATTTCGATGA TGCAGCTTGT GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGGAGCC   961 GGGACTGTCG GGCGTACACA AATCGCCCGC AGAAGCGCGG GCCGTCTGGG ACCCGATGGC  1021 TGTGTAGAAG TACTCGCGAT AGTGGAAACC GACGCCCCAG CACTTCGTTC GAGGCCAAAG 1081 GAAATAGATC GATCAAATTA AGATTTTTAT TTAGGTCTCC AGAAAAAAGT TG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl