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pMSCV-GFP-hCep63 (#RDB15403)

Retroviral expression vector of human Cep63 fused with EGFP at N-terminal.

Clone info. Retroviral expression vector of human Cep63 fused with EGFP at N-terminal.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pMSCV–GFP–Zeo (plasmid)
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name human CEP63 cDNA
Depositor|Developer Shimizu, Shigeomi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15403 pMSCV-GFP-hCep63 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMSCV-GFP-hCep63 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15403).

Reference section:

Watanabe, Y., Honda, S., Konishi, A., Arakawa, S., Murohashi, M., Yamaguchi, H., Torii, S., Tanabe, M., Tanaka, S., Warabi, E., Shimizu, S., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15403_A7Ikp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: gag_t-C_F (Pr0730)
Region: gag 3', EGFP, insert 5'
Sequence file: RDB15403_A7Ika.seq check
>D04516A3_A7Ik_1_gag_t-C_F_C02_03_ABI08.ab1
    1 ACCGGGAAAC TCCTTTATCC AGCCCTCACT CCTTCTCTAG GCGCCGGAAT TAGATCCATG
   61 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
  121 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
  181 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
  241 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG
  301 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
  361 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
  421 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
  481 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC
  541 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
  601 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
  661 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
  721 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGAGATCC
  781 ATGGAGGCTT TGTTAGAAGG AATACAAAAT CGAGGGCATG GTGGGGGATT TTTGACATCT
  841 TGTGAAGCAG AACTACAGGA GCTCATGAAA CAGATTGACA TAAATGGTGG CTCATAAAAA
  901 ATCTGAATGG GGAAGGACGT ACACATGCTC TAGAAACTTT GCTTGAAAAT CCGTGAACAG
  961 GAACTTAAAG AGTCTTAGGA GTCAGTTGGA TGTGACACAT AAAGGAAGGT TGGAAATGTT
 1021 GCATCAGCAG GTAGAAGGAA CATGAAAAAA TCAAGCAAGA GATGACCATT GGAATATAGC
1081 AAGCAGTTTG AAAGAAACCT ACATGTGAAG AGAT
//
Primer: EGFP-C (Pr0025)
Region: EGFP3', insert5'
Sequence file: RDB15403_A7Ikb.seq check
>D04516A3_A7Ik_1_EGFP_C_C05_08_ABI24.ab1
    1 CAGCGGGAAA CCATTCTCGG GGCATGGGGA CGAGCTGTAC AAGAGATCCA TGGAGGCTTT
   61 GTTAGAAGGA ATACAAAATC GAGGGCATGG TGGGGGATTT TTGACATCTT GTGAAGCAGA
  121 ACTACAGGAG CTCATGAAAC AGATTGACAT AATGGTGGCT CATAAAAAAT CTGAATGGGA
  181 AGGACGTACA CATGCTCTAG AAACTTGCTT GAAAATCCGT GAACAGGAAC TTAAGAGTCT
  241 TAGGAGTCAG TTGGATGTGA CACATAAGGA GGTTGGAATG TTGCATCAGC AGGTAGAAGA
  301 ACATGAAAAA ATCAAGCAAG AGATGACCAT GGAATATAAG CAGGAGTTGA AGAAACTACA
  361 TGAAGAATTA TGCATACTGA AGAGAAGCTA TGAAAAGCTT CAGAAAAAGC AAATGAGGGA
  421 ATTCAGAGGA AATACCAAAA ATCACAGGGA AGATCGGTCT GAAATTGAGA GGTTAACTGC
  481 AAAAATAGAG GAATTCCGTC AGAAATCGCT GGACTGGGAG AAGCAACGCT TGATTTATCA
  541 GCAACAGGTA TCTTCACTGG AGGCACAAAG GAAGGCTCTG GCTGAACAAT CAGAGATAAT
  601 TCAGGCTCAG CTTGTCAATC GGAAACAGAA ATTAGAGTCT GTGGAACTTT CTAGCCAATC
  661 AGAAATTCAA CACTTAAGCA GTAAACTGGA GCGGGCTAAT GACACTATCT GTGCCAATGA
  721 GTTGGAAATA GAGCGCCTCA CCATGAGGGT CAATGACTTG GTTGGAACCA GTATGACTGT
  781 CCTACAGGAG CAGCAGCAAA AAGAAGAAAA ATTGAGGGAA TCTGAAAAAC TATTAGAGGC
  841 TCTGCAGGAA GAAAAGAGAG AATTGAAGGC AGCTCTTCAG TCTCAAGAAA ATCTCATACA
  901 TGAGGCCAGA ATACAAAAGG AGAAGTTACA AGGAAAAAGT AAAAGGCAAC TAACACTCAA
  961 CATGCTGTAG AAGCTATAAA GGCCACGGGA AGAATCTCTG GCAGAAAAGA AGTACCACCT
 1021 TCTCAGGGCA GGGGACTTTA GACAGTGTGC CTCCTCCCCA GTTGAATTTT ACCCATACTA
 1081 GTGAGGACCT TTCTGGCAGC AGAGTGACTT GTTCTTGAGC CAGTTGATCT TGTGGATGCC
1141 AACGGTGTTA AACCAGCCCT GGAGGCCCAC AGA
//
Primer: PGK_N_rev (Pr0243)
Region: insert3'
Sequence file: RDB15403_A7Ikc.seq check
>D04516A3_A7Ik_1_PGK_N_rev_G05_20_ABI24.ab1
    1 CATTGGACGC TCCCTACCCG GTAGATTCGT TAACCTCGAG CTACTTTAAG GCTGTGAATT
   61 GTCTCACTGT CTTTTCACTC TCTCTTTTTA GTTCTTCAAT ATGGGCATCC AAGCGCTCTA
  121 GAATGTGATG AGACCTCAGT TCCTCCTCTT CCAAAAATCT GGTAGCTATT GAACCAAGGG
  181 GAGATACAGG CAAGGAACAC GAAGATATAA ACTCTTCTTG GTCATTCATA CTCTCAGACA
  241 TAGTGTCAGA AAAATTGGCT TCATTTGTAT CTAGCGCTGA AGGCAAAGAA TGAGTTTTAC
  301 ATAGAGAGTA GGAAGTCAAA GACCTGGTGG AGCTGCAAGG GCTGATTTGA GGACTCAGGG
  361 GGCTTAGCAC CCTAGACATG GGGTTTATAC TATCCGACGC TTGTCCTCTT GGAGAATGAA
  421 GATCTGTTTT GTAGTGCTCA GTCTTTATTC CATCATGTCT GTGCTGGCCA TGGGTTGGCT
  481 TGAACTCTGT ATTCTTGAAC TCAGTTGTTC TGCTGTGTGT TGGTTTAAAG ATCCTGTCAT
  541 TTTGTTTTTT GCACATCTGA GTAGAAATCT CCAGCTGAGA TTTGGTATTC ATCAAATCTT
  601 TCTGTAGTTG TTGATTTTCA GACACTAATT TATTTAATGC CTCAATATAA TTCTCCTGGA
  661 GGTCTGCTAG TGAAATCTCT TTTGCCTCAT GTAAACCCAA TTCCAATTTC ATCACCATTT
  721 CAGAAAGATG ACGATTTTCT AATTTGAGTG ACTCCAGCTG ATCCAAAATC TCCTTATGCT
  781 CTACTGCTTT GTCTTCTGCC TTTTGCATCT CTGCTCTGAG TCGCTTTTCC ATGTCTGAGG
  841 AAGAACCTTT GGTGGAAGCA ATAGTGATAT CTCGCTGATG TAACTCCTGA GTTAGATGGG
  901 AGATTTCCAT CTTCATTCCT TCTAGTGCAG AACTGTAACT TTGTTCACCC TGTAAAATCT
  961 GTTCTTTCAA CTTCTTAATC TCTGCTTTGT ATTCATTGTT ATGTGCTTTC CATCCCTCTT
 1021 CAGTTCTTTC ATATTTTTCC ATTTAGACTT GGCTCAGCTG TTTTACACGT GGCACTCACA
 1081 GATTTCCCAA ACTGTCTCAG ACCAGTTCAC CTCTGGCCTG CAGAAGGATC CTCCACTAGG
1141 TAATTGCGT
//
Primer: pBR322_BamHI_R (Pr0065)
Region: 5'LTR, MESV psi
Sequence file: RDB15403_A7Ikd.seq check
>D04516A3_A7Ik_1_pBR322_BamHI_R_E05_14_ABI24.ab1
    1 TGTTTAAGGA AGGATATCAG TGGTCCAGGC TCTAGTTTTG ACTCAACAAT ATCACCAGCT
   61 GAAGCCTATA GAGTACGAGC CATAGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA
  121 GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG
  181 CAAGGCATGG AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTT AGGAACAGAG
  241 AGACAGCAGA ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG
  301 GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCCGCCCTCA GCAGTTTCTA GAGAACCATC
  361 AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA
  421 ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC
  481 CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATAGACTG CGTCGCCCGG GTACCCGTAT
  541 TCCCAATAAA GCCTCTTGCT GTTTGCATCC GAATCGTGGA CTCGCTGATC CTTGGGAGGG
  601 TCTCCTCAGA TTGATTGACT GCCCACCTCG GGGGTCTTTC ATTTGGAGGT TCCACCGAGA
  661 TTTGGAGACC CCTGCCCAGG GACCACCGAC CCCCCCGCCG GGAGGTAAGC TGGCCAGCGG
  721 TCGTTTCGTG TCTGTCTCTG TCTTTGTGCG TGTTTGTGCC GGCATCTAAT GTTTGCGCCT
  781 GCGTCTGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG GAACTGACGA
  841 GTTCTGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTTGGGG GCCGTTTTTG
  901 TGGCCCGACC TGAGGAAGGG AGTCGATGTG GAATCCGACC CCGTCAGGAT ATGTGGTTCT
  961 GGTAGGAGAC GAGAACCTAA AACAGTTTCC CGCCTCCGTC TGAAATTTTT TGCTTTCGGG
 1021 TTTGGAACCC GAAAGCCCGC GCGGTCTTGT CTGCTGCAGC GCTGCAGCAT CGATTCTGTG
 1081 ATGTCTCTGT CTGACTGTGT TTCCTGTATT TGGTCCTGAA AATTAGATCA GACTGATTAC
1141 CCACTCCCCT TTTAAGGGGT T
//
Primer: EGFP-N (Pr0027)
Region: EGFP, gag, MESV psi, 5' LTR
Sequence file: RDB15403_A7Ike.seq check
>D04516A3_A7Ik_1_EGFP_N_D05_11_ABI24.ab1
    1 GTAAACCGGG GGGGGACAGC TCCTCGCCCT TGCTCACCAT GGATCTAATT CCGGCGCCTA
   61 GAGAAGGAGT GAGGGCTGGA TAAAGGGAGG ATCGAGGCGG GGTCGAACGA GGAGGTTCAA
  121 GGGGGAGAGA CGGGGCGGAT GGAGGAAGAG GAGGCGGAGG CTTAGGGTGT ACAAAGGGCT
  181 TGACCCAGGG AGGGGGGTCA AAAGCCAAGG CTTCCCAGGT CACGATGTAG GGGACCTGGT
  241 CTGGGTGTCC ATGCGGGCCA GGTGAAAAGA CCTTGATCTT AACCTGGGTG ATGAGGTCTC
  301 GGTTAAAGGT GCCGTCTCGC GGCCATCCGA CGTTAAAGGT TGGCCATTCT GCAGAGCAGA
  361 AGGTAACCCA ACGTCTCTTC TTGACATCTA CCGACTGGTT GTGAGCGATC CGCTCGACAT
  421 CTTTCCAGTG ACCTAAGGTC AAACTTAAGG GAGTGGTAAC AGTCTGGCCC TAATTTTCAG
  481 ACAAATACAG AAACACAGTC AGACAGAGAC AACACAGAAC GATGCTGCAG CGCTGCAGCA
  541 GACAAGACGC GCGGCTTCGG TTCCAAACCG AAAGCAAAAA TTCAGACGGA GGCGGGAACT
  601 GTTTTAGGTT CTCGTCTCCT ACCAGAACCA CATATCCTGA CGGGGTCGGA TTCCACATCG
  661 ACTCCCTTCC TCAGGTCGGG CCACAAAAAC GGCCCCCAAA GTCCCTGGGA CGTCTCCCAG
  721 GGTTGCGGCC GGGTGTTCAG AACTCGTCAG TTCCACCACG GGTCCGCCAG ATACAGAGCT
  781 AGTTAGCTAA CTAGTACAGA CGCAGGCGCA AACATTAGAT GCCGGCACAA ACACGCACAA
  841 AGACAGAGAC AGACACGAAA CGACCGCTGG CCAGCTTACC TCCCGGCGGG GGGGTCGGTG
  901 GTCCCTGGGC AGGGGTCTCC AAATCTCGGT GGAACCTCCA AATGAAAGAC CCCCGAGGTG
  961 GCAGTCAATC AATCTGAGGA GACCCTCCCA AGGATCAGCG AGTCCACGAT TCGATGCAAA
 1021 ACAGCAAGAG CTTTTATTGG AATACGGTAC CCGGCGACGC AGTCTATCGG AGACTGCGCG
 1081 TGAGTGAGGA TTGTGGCTCT TTAATGACTC GGGAGCAGAA GCGGCGCGAA CCAGAAGTCG
1141 C
//
Primer: PGKpro_F (Pr0249)
Region: PGK pro, HygR, 3'LTR
Sequence file: RDB15403_A7Ikf.seq check
>D04516A3_A7Ik_1_PGKpro_F_A07_01_ABI24.ab1
    1 AGGGGGGCCG TATCTCTCTT CTCATCTCCG GGCCTTTCGA CCTGCAGCCC AAGCTTATGA
   61 AAAAGCCTGA ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG
  121 TCTCCGACCT GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG
  181 GAGGGCGTGG ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT
  241 ATGTTTATCG GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG
  301 AGTTCAGCGA GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG
  361 ACCTGCCTGA AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA
  421 TCGCTGCGGC CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG
  481 GTCAATACAC TACATGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT
  541 GGCAAACTGT GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA
  601 TGCTTTGGGC CGAGGACTGC CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA
  661 ACAATGTCCT GACGGACAAT GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT
  721 TCGGGGATTC CCAATACGAG GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA
  781 TGGAGCAGCA GACGCGCTAC TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCGCGGC
  841 TCCGGGCGTA TATGCTCCGC ATTGGTCTTG ACCAACTCTA TCAGAGCTTG GTTGACGGCA
  901 ATTTCGATGA TGCAGCTTGT GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGGAGCC
  961 GGGACTGTCG GGCGTACACA AATCGCCCGC AGAAGCGCGG GCCGTCTGGG ACCCGATGGC
 1021 TGTGTAGAAG TACTCGCGAT AGTGGAAACC GACGCCCCAG CACTTCGTTC GAGGCCAAAG
1081 GAAATAGATC GATCAAATTA AGATTTTTAT TTAGGTCTCC AGAAAAAAGT TG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Watanabe, Y., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]