Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMSCV-GFP-mCentrin2 (#RDB15401)

Retroviral expression vector of mouse Centrin2 fused with EGFP at N-terminal.

Clone info. Retroviral expression vector of mouse Centrin2 fused with EGFP at N-terminal.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pMSCV–GFP–Zeo (plasmid)
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name mouse Cetn2 cDNA
Depositor|Developer Shimizu, Shigeomi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15401 pMSCV-GFP-mCentrin2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMSCV-GFP-mCentrin2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15401).

Reference section:

Watanabe, Y., Honda, S., Konishi, A., Arakawa, S., Murohashi, M., Yamaguchi, H., Torii, S., Tanabe, M., Tanaka, S., Warabi, E., Shimizu, S., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15401_A7Ikp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',insert 5'(Centrin2,PGK pro)
Sequence file: RDB15401_A7Ikf.seq check
>15401_15401_B2GlF_mVenus-M_F_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTCNAGG TGANNTTNNA GATCCGCCAC ANNCATCGAG
   61 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC
  121 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC
  181 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC
  241 ATGGACGAGC TGTACAAGAG ATCTGCCTCT AATTTTAAGA AGACAACCAT GGCATCCAGT
  301 GCCCAGAGAA AAAGGATGAG TCCTAAGCCT GAACTTACTG AAGACCAGAA ACAGGAAATC
  361 CGGGAAGCTT TTGATCTCTT TGATGCTGAT GGAACTGGAA CTATAGACAT CAAAGAACTG
  421 AAGGTGGCAA TGAGGGCCCT GGGCTTTGAG CCCAAGAAAG AAGAAATTAA GAAAATGATA
  481 AGTGAAATTG ATAAAGAAGG AACAGGAAAA ATGAACTTCA GTGACTTTTT GACAGTGATG
  541 ACTCAGAAAA TGTCTGAGAA AGACACTAAA GAAGAAATCC TGAAAGCTTT CAAGTTGTTC
  601 GATGATGATG AGACTGGGAA AATATCATTC AAGAATCTTA AGCGCGTGGC CAAGGAGCTG
  661 GGTGAGAACC TGACAGATGA GGAACTGCAG GAAATGATTG ATGAAGCTGA TCGAGATGGA
  721 GATGGTGAGG TCAATGAGCA AGAGTTCCTG CGCATCATGA AAAAGACCAG CCTCTATTAA
  781 GAATTCTACC GGGTAGGGGA GGCGCTTTTC CCNAGGCAGT CTGGAGCATG CGCTTTANCA
  841 GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCNGGC CTCNCACACA TTCCACATCC
  901 NCCGGTANGC GCCNNNNGGC TNCGTTNNTT GGNGGNCCCT TCGCGCCACN NNCTACTCCN
  961 CCCCNANNCN NGNNNNNCCC CCCNNNCCCN NNNNNNNGNN NNCNNGNAGN NNGNNNCAAA
1021 NNGNNNNNNN CANNNTNNNN NTANNNNNNN NGCNNANNNN NNNNNN
//
Primer: PGKpro_F (Pr0249)
Region: Zeo,3' LTR
Sequence file: RDB15401_A7Ikg.seq check
>D04516A1_A7Ik_2_PGKpro_F_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTCTCC GGGCCTTTCG ACCTGCAGCN NNNNCTTATG
   61 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG
  121 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG
  181 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC
  241 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC
  301 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG
  361 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG
  421 GAGCAGGACT GAATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT
  481 GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG
  541 GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA GAGACAGCAG
  601 AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA NGGCCAAGAA
  661 CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC
  721 NAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG
  781 CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGANCTCA ATAAAAGANC CCACAACCCC
  841 TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCNCCCG GGNACCCGNG TATCNNANAA
  901 ACCCTCTTGC ANTTGCATNC NNACTTGNGG TCNCGCTGTT CCTNGGGNNG GTCTNNTCNN
961 ANNGANTGAC TANNNNNNCA NCNGGGGGNN TTTCNTGGNN TNNNANNTTN NNNNNNNNN
//
Primer: EGFP-N (Pr0027)
Region: gag,MESV_psi
Sequence file: RDB15401_A7Ikh.seq check
>D04516A1_A7Ik_2_EGFP_N_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN NCGCNNTNGC TNNCNNTGGN TCTAATTCCG GCGCCTAGAG
   61 AAGGAGTGAG GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG
  121 GGAGAGACGG GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA
  181 CCCAGGGAGG GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG
  241 GGTGTCCATG CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT
  301 TAAAGGTGCC GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG
  361 TAACCCAACG TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT
  421 TCCAGTGACC TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCTAA TTTTCAGACA
  481 AATACAGAAA CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCGC TGCAGCAGAC
  541 AAGACGCGCG GCTTCGGTTC CAAACCGAAA GCAAAAATTC AGACGGAGGC GGGAACTGTT
  601 TTAGGTTCTC GTCTCCTACC AGAACCACAT ATCCTGACGG GGTCGGATTC CACATCGACT
  661 CCCTTCCTCA GGTCGGGCCA CAAAAACGGC CCCCAAAGTC CCTGGGACGT CTCCCAGGGT
  721 TGCGGCCGGG TGTTCAGAAC TCGTCAGTTC CACCACGGGT CCGCCAGATA CAGAGCTAGT
  781 TAGCTAACTA GTACAGACGC ANGCGCAAAC ATTAGATGCC GGCNCAAACA CGCACAAAGA
  841 CNGAGACAGA CACGAAACGA CCGCTGGCCA GCTTACNNNC CGGCGGGGGG GGTCGGNGGN
  901 NCCNNGGCAN GGGNCTNCNA ATCTCNNNGG AACNNNCNAN NGAAANANCC CCNNNNNGGN
  961 NANNNNNTCN NTCTGNNNAN ANCNNNCNNN NGNATCANCN NNTCNNCNNN TNNGNNNGNN
1021 NNNNNNNN
//
Primer: gag-M_F (Pr0471)
Region: gag,EGFP 5'
Sequence file: RDB15401_A7Iki.seq check
>15401_15401_B2GlF_gag-M_F_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNTNN NNNNNNCTNN NNNNCCGCCT CCTCTTCCTC CNTCCGCCCC
   61 GTCTCTCCCC CTTGAACCTC CTCGTTCGAC CCCGCCTCGA TCCTCCCTTT ATCCAGCCCT
  121 CACTCCTTCT CTAGGCGCCG GAATTAGATC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC
  181 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT
  241 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC
  301 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA
  361 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC
  421 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG
  481 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA
  541 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA
  601 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA
  661 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG
  721 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGANC ACCCAGTCCG CCCTGANCAA
  781 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT
  841 CACTCTCNGC ATGGACGANC TGTACNAGAG ATCTGCCNCT AATTTTAANA NGANNACCAT
  901 GGCATNCANN GNCCAGANAA ANNGNNGANT CCTANGCCTG AANTTANNGN ANANCNNAAA
  961 CAGGNAATCN NGNANGNTNN NNTNTCNTTG ATGCTGATNG NANTNGNANN TATNNACNTN
1021 NAAGNNNNNN NNNN
//
Primer: pBR322_BamHI_R (Pr0065)
Region: 5' LTR,MESV_psi
Sequence file: RDB15401_A7Ikj.seq check
>D04516A1_A7Ik_2_pBR322_BamHI_R_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGGTCNNG GCTCTAGTTT TGACTCNACN ATATCACCAG
   61 CTGAAGCCTA TAGAGTACGA GCCATAGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA
  121 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
  181 TGCAAGGCAT GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG
  241 AGAGACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
  301 AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA
  361 TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC
  421 CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG
  481 CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT
  541 ATTCCCAATA AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG
  601 GGTCTCCTCA GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA
  661 GATTTGGAGA CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC
  721 GGTCGTTTCG TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGNTTGCGC
  781 CTGCGTCTGT ACTAGTTAGC TAACTAGCTC TGTATCTGGN GGACCCGTGG TGGAACTGAC
  841 GAGTTCTGAA CACCCGGNCG CAACCCNGGG NNANGTCCCA NGGNNTTNGG GGGGCCGTTT
  901 TTGNGGNCCG ANCNNAGGNA NNGNANTCNA NGNNGAATNC NANCCNNNCN NGNNANGTGG
  961 NTCTGGNANG ANANNANAAN CTAAAACNNN NNCCGCCNNN NNCTGNANTT TTTNNNTTNN
1021 GNNTNGNNAN NNNNANNCNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Watanabe, Y., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]