Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMSCV-GFP-mCentrin2

Retroviral expression vector of mouse Centrin2 fused with EGFP at N-terminal.

Catalog number RDB15401
Resource name pMSCV-GFP-mCentrin2
Clone info. Retroviral expression vector of mouse Centrin2 fused with EGFP at N-terminal.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pMSCV–GFP–Zeo (plasmid)
Selectable markers Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name mouse Cetn2 cDNA
Depositor|Developer Shimizu, Shigeomi |
Other clones in our bank

External Database
mouse Cetn2

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15401 pMSCV-GFP-mCentrin2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Watanabe, Y., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15401_A7Ikp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: mVenus-M_F
          Region: EGFP 3',insert 5'(Centrin2,PGK pro)
          Sequence file: RDB15401_A7Ikf.seq check
          >15401_15401_B2GlF_mVenus-M_F_B02_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTCNAGG TGANNTTNNA GATCCGCCAC ANNCATCGAG
             61 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC
            121 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC
            181 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC
            241 ATGGACGAGC TGTACAAGAG ATCTGCCTCT AATTTTAAGA AGACAACCAT GGCATCCAGT
            301 GCCCAGAGAA AAAGGATGAG TCCTAAGCCT GAACTTACTG AAGACCAGAA ACAGGAAATC
            361 CGGGAAGCTT TTGATCTCTT TGATGCTGAT GGAACTGGAA CTATAGACAT CAAAGAACTG
            421 AAGGTGGCAA TGAGGGCCCT GGGCTTTGAG CCCAAGAAAG AAGAAATTAA GAAAATGATA
            481 AGTGAAATTG ATAAAGAAGG AACAGGAAAA ATGAACTTCA GTGACTTTTT GACAGTGATG
            541 ACTCAGAAAA TGTCTGAGAA AGACACTAAA GAAGAAATCC TGAAAGCTTT CAAGTTGTTC
            601 GATGATGATG AGACTGGGAA AATATCATTC AAGAATCTTA AGCGCGTGGC CAAGGAGCTG
            661 GGTGAGAACC TGACAGATGA GGAACTGCAG GAAATGATTG ATGAAGCTGA TCGAGATGGA
            721 GATGGTGAGG TCAATGAGCA AGAGTTCCTG CGCATCATGA AAAAGACCAG CCTCTATTAA
            781 GAATTCTACC GGGTAGGGGA GGCGCTTTTC CCNAGGCAGT CTGGAGCATG CGCTTTANCA
            841 GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCNGGC CTCNCACACA TTCCACATCC
            901 NCCGGTANGC GCCNNNNGGC TNCGTTNNTT GGNGGNCCCT TCGCGCCACN NNCTACTCCN
            961 CCCCNANNCN NGNNNNNCCC CCCNNNCCCN NNNNNNNGNN NNCNNGNAGN NNGNNNCAAA
          1021 NNGNNNNNNN CANNNTNNNN NTANNNNNNN NGCNNANNNN NNNNNN
          //
          Primer: PGKpro_F
          Region: Zeo,3' LTR
          Sequence file: RDB15401_A7Ikg.seq check
          >D04516A1_A7Ik_2_PGKpro_F_E04_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNTCTCC GGGCCTTTCG ACCTGCAGCN NNNNCTTATG
             61 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG
            121 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG
            181 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC
            241 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC
            301 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG
            361 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG
            421 GAGCAGGACT GAATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT
            481 GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG
            541 GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA GAGACAGCAG
            601 AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA NGGCCAAGAA
            661 CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC
            721 NAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG
            781 CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGANCTCA ATAAAAGANC CCACAACCCC
            841 TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCNCCCG GGNACCCGNG TATCNNANAA
            901 ACCCTCTTGC ANTTGCATNC NNACTTGNGG TCNCGCTGTT CCTNGGGNNG GTCTNNTCNN
          961 ANNGANTGAC TANNNNNNCA NCNGGGGGNN TTTCNTGGNN TNNNANNTTN NNNNNNNNN
          //
          Primer: EGFP-N
          Region: gag,MESV_psi
          Sequence file: RDB15401_A7Ikh.seq check
          >D04516A1_A7Ik_2_EGFP_N_C04_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCNNN NCGCNNTNGC TNNCNNTGGN TCTAATTCCG GCGCCTAGAG
             61 AAGGAGTGAG GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG
            121 GGAGAGACGG GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA
            181 CCCAGGGAGG GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG
            241 GGTGTCCATG CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT
            301 TAAAGGTGCC GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG
            361 TAACCCAACG TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT
            421 TCCAGTGACC TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCTAA TTTTCAGACA
            481 AATACAGAAA CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCGC TGCAGCAGAC
            541 AAGACGCGCG GCTTCGGTTC CAAACCGAAA GCAAAAATTC AGACGGAGGC GGGAACTGTT
            601 TTAGGTTCTC GTCTCCTACC AGAACCACAT ATCCTGACGG GGTCGGATTC CACATCGACT
            661 CCCTTCCTCA GGTCGGGCCA CAAAAACGGC CCCCAAAGTC CCTGGGACGT CTCCCAGGGT
            721 TGCGGCCGGG TGTTCAGAAC TCGTCAGTTC CACCACGGGT CCGCCAGATA CAGAGCTAGT
            781 TAGCTAACTA GTACAGACGC ANGCGCAAAC ATTAGATGCC GGCNCAAACA CGCACAAAGA
            841 CNGAGACAGA CACGAAACGA CCGCTGGCCA GCTTACNNNC CGGCGGGGGG GGTCGGNGGN
            901 NCCNNGGCAN GGGNCTNCNA ATCTCNNNGG AACNNNCNAN NGAAANANCC CCNNNNNGGN
            961 NANNNNNTCN NTCTGNNNAN ANCNNNCNNN NGNATCANCN NNTCNNCNNN TNNGNNNGNN
          1021 NNNNNNNN
          //
          Primer: gag-M_F
          Region: gag,EGFP 5'
          Sequence file: RDB15401_A7Iki.seq check
          >15401_15401_B2GlF_gag-M_F_C02_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNTNN NNNNNNCTNN NNNNCCGCCT CCTCTTCCTC CNTCCGCCCC
             61 GTCTCTCCCC CTTGAACCTC CTCGTTCGAC CCCGCCTCGA TCCTCCCTTT ATCCAGCCCT
            121 CACTCCTTCT CTAGGCGCCG GAATTAGATC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC
            181 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT
            241 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC
            301 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA
            361 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC
            421 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG
            481 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA
            541 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA
            601 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA
            661 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG
            721 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGANC ACCCAGTCCG CCCTGANCAA
            781 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT
            841 CACTCTCNGC ATGGACGANC TGTACNAGAG ATCTGCCNCT AATTTTAANA NGANNACCAT
            901 GGCATNCANN GNCCAGANAA ANNGNNGANT CCTANGCCTG AANTTANNGN ANANCNNAAA
            961 CAGGNAATCN NGNANGNTNN NNTNTCNTTG ATGCTGATNG NANTNGNANN TATNNACNTN
          1021 NAAGNNNNNN NNNN
          //
          Primer: pBR322_BamHI_R
          Region: 5' LTR,MESV_psi
          Sequence file: RDB15401_A7Ikj.seq check
          >D04516A1_A7Ik_2_pBR322_BamHI_R_D04_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGGTCNNG GCTCTAGTTT TGACTCNACN ATATCACCAG
             61 CTGAAGCCTA TAGAGTACGA GCCATAGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA
            121 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
            181 TGCAAGGCAT GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG
            241 AGAGACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
            301 AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA
            361 TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC
            421 CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG
            481 CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT
            541 ATTCCCAATA AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG
            601 GGTCTCCTCA GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA
            661 GATTTGGAGA CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC
            721 GGTCGTTTCG TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGNTTGCGC
            781 CTGCGTCTGT ACTAGTTAGC TAACTAGCTC TGTATCTGGN GGACCCGTGG TGGAACTGAC
            841 GAGTTCTGAA CACCCGGNCG CAACCCNGGG NNANGTCCCA NGGNNTTNGG GGGGCCGTTT
            901 TTGNGGNCCG ANCNNAGGNA NNGNANTCNA NGNNGAATNC NANCCNNNCN NGNNANGTGG
            961 NTCTGGNANG ANANNANAAN CTAAAACNNN NNCCGCCNNN NNCTGNANTT TTTNNNTTNN
          1021 GNNTNGNNAN NNNNANNCNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Watanabe, Y., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116.

          2022.08.11

          GNP_filter3_RDBDEP_html_220808.pl