RDB15401 - pMSCV-GFP-mCentrin2

Please review the QC test results indicated by check

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pMSCV-GFP-mCentrin2 (#RDB15401)

Retroviral expression vector of mouse Centrin2 fused with EGFP at N-terminal.

Clone info.	Retroviral expression vector of mouse Centrin2 fused with EGFP at N-terminal.
Comment	Expression was confirmed by the depositor with immunostaining.
Vector backbone	pMSCV–GFP–Zeo (plasmid)
Selectable markers	Amp^r (E. coli), Zeo^r (mammalian cells).
Gene/insert name	mouse Cetn2 cDNA
Depositor\|Developer	Shimizu, Shigeomi \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15401	pMSCV-GFP-mCentrin2	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMSCV-GFP-mCentrin2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15401).

Reference section:

Watanabe, Y., Honda, S., Konishi, A., Arakawa, S., Murohashi, M., Yamaguchi, H., Torii, S., Tanabe, M., Tanaka, S., Warabi, E., Shimizu, S., Autophagy controls centrosome number by degrading Cep63. Nat. Commun. 7: 13508 (2016). PMID 27869116. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15401_A7Ikp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F (Pr0574) Region: EGFP 3',insert 5'(Centrin2,PGK pro) Sequence file: RDB15401_A7Ikf.seq
>15401_15401_B2GlF_mVenus-M_F_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNTCNAGG TGANNTTNNA GATCCGCCAC ANNCATCGAG 61 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC 121 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC 181 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC 241 ATGGACGAGC TGTACAAGAG ATCTGCCTCT AATTTTAAGA AGACAACCAT GGCATCCAGT 301 GCCCAGAGAA AAAGGATGAG TCCTAAGCCT GAACTTACTG AAGACCAGAA ACAGGAAATC 361 CGGGAAGCTT TTGATCTCTT TGATGCTGAT GGAACTGGAA CTATAGACAT CAAAGAACTG 421 AAGGTGGCAA TGAGGGCCCT GGGCTTTGAG CCCAAGAAAG AAGAAATTAA GAAAATGATA 481 AGTGAAATTG ATAAAGAAGG AACAGGAAAA ATGAACTTCA GTGACTTTTT GACAGTGATG 541 ACTCAGAAAA TGTCTGAGAA AGACACTAAA GAAGAAATCC TGAAAGCTTT CAAGTTGTTC 601 GATGATGATG AGACTGGGAA AATATCATTC AAGAATCTTA AGCGCGTGGC CAAGGAGCTG 661 GGTGAGAACC TGACAGATGA GGAACTGCAG GAAATGATTG ATGAAGCTGA TCGAGATGGA 721 GATGGTGAGG TCAATGAGCA AGAGTTCCTG CGCATCATGA AAAAGACCAG CCTCTATTAA 781 GAATTCTACC GGGTAGGGGA GGCGCTTTTC CCNAGGCAGT CTGGAGCATG CGCTTTANCA 841 GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCNGGC CTCNCACACA TTCCACATCC 901 NCCGGTANGC GCCNNNNGGC TNCGTTNNTT GGNGGNCCCT TCGCGCCACN NNCTACTCCN 961 CCCCNANNCN NGNNNNNCCC CCCNNNCCCN NNNNNNNGNN NNCNNGNAGN NNGNNNCAAA 1021 NNGNNNNNNN CANNNTNNNN NTANNNNNNN NGCNNANNNN NNNNNN //
Primer: PGKpro_F (Pr0249) Region: Zeo,3' LTR Sequence file: RDB15401_A7Ikg.seq
>D04516A1_A7Ik_2_PGKpro_F_E04_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNTCTCC GGGCCTTTCG ACCTGCAGCN NNNNCTTATG 61 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG 121 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG 181 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC 241 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC 301 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG 361 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG 421 GAGCAGGACT GAATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT 481 GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG 541 GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA GAGACAGCAG 601 AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA NGGCCAAGAA 661 CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC 721 NAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG 781 CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGANCTCA ATAAAAGANC CCACAACCCC 841 TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCNCCCG GGNACCCGNG TATCNNANAA 901 ACCCTCTTGC ANTTGCATNC NNACTTGNGG TCNCGCTGTT CCTNGGGNNG GTCTNNTCNN 961 ANNGANTGAC TANNNNNNCA NCNGGGGGNN TTTCNTGGNN TNNNANNTTN NNNNNNNNN //
Primer: EGFP-N (Pr0027) Region: gag,MESV_psi Sequence file: RDB15401_A7Ikh.seq
>D04516A1_A7Ik_2_EGFP_N_C04_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNCNNN NCGCNNTNGC TNNCNNTGGN TCTAATTCCG GCGCCTAGAG 61 AAGGAGTGAG GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG 121 GGAGAGACGG GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA 181 CCCAGGGAGG GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG 241 GGTGTCCATG CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT 301 TAAAGGTGCC GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG 361 TAACCCAACG TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT 421 TCCAGTGACC TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCTAA TTTTCAGACA 481 AATACAGAAA CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCGC TGCAGCAGAC 541 AAGACGCGCG GCTTCGGTTC CAAACCGAAA GCAAAAATTC AGACGGAGGC GGGAACTGTT 601 TTAGGTTCTC GTCTCCTACC AGAACCACAT ATCCTGACGG GGTCGGATTC CACATCGACT 661 CCCTTCCTCA GGTCGGGCCA CAAAAACGGC CCCCAAAGTC CCTGGGACGT CTCCCAGGGT 721 TGCGGCCGGG TGTTCAGAAC TCGTCAGTTC CACCACGGGT CCGCCAGATA CAGAGCTAGT 781 TAGCTAACTA GTACAGACGC ANGCGCAAAC ATTAGATGCC GGCNCAAACA CGCACAAAGA 841 CNGAGACAGA CACGAAACGA CCGCTGGCCA GCTTACNNNC CGGCGGGGGG GGTCGGNGGN 901 NCCNNGGCAN GGGNCTNCNA ATCTCNNNGG AACNNNCNAN NGAAANANCC CCNNNNNGGN 961 NANNNNNTCN NTCTGNNNAN ANCNNNCNNN NGNATCANCN NNTCNNCNNN TNNGNNNGNN 1021 NNNNNNNN //
Primer: gag-M_F (Pr0471) Region: gag,EGFP 5' Sequence file: RDB15401_A7Iki.seq
>15401_15401_B2GlF_gag-M_F_C02_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNTNN NNNNNNCTNN NNNNCCGCCT CCTCTTCCTC CNTCCGCCCC 61 GTCTCTCCCC CTTGAACCTC CTCGTTCGAC CCCGCCTCGA TCCTCCCTTT ATCCAGCCCT 121 CACTCCTTCT CTAGGCGCCG GAATTAGATC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC 181 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT 241 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC 301 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA 361 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC 421 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG 481 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA 541 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA 601 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA 661 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG 721 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGANC ACCCAGTCCG CCCTGANCAA 781 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT 841 CACTCTCNGC ATGGACGANC TGTACNAGAG ATCTGCCNCT AATTTTAANA NGANNACCAT 901 GGCATNCANN GNCCAGANAA ANNGNNGANT CCTANGCCTG AANTTANNGN ANANCNNAAA 961 CAGGNAATCN NGNANGNTNN NNTNTCNTTG ATGCTGATNG NANTNGNANN TATNNACNTN 1021 NAAGNNNNNN NNNN //
Primer: pBR322_BamHI_R (Pr0065) Region: 5' LTR,MESV_psi Sequence file: RDB15401_A7Ikj.seq
>D04516A1_A7Ik_2_pBR322_BamHI_R_D04_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGGTCNNG GCTCTAGTTT TGACTCNACN ATATCACCAG 61 CTGAAGCCTA TAGAGTACGA GCCATAGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA 121 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT 181 TGCAAGGCAT GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG 241 AGAGACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC 301 AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA 361 TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC 421 CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG 481 CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT 541 ATTCCCAATA AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG 601 GGTCTCCTCA GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA 661 GATTTGGAGA CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC 721 GGTCGTTTCG TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGNTTGCGC 781 CTGCGTCTGT ACTAGTTAGC TAACTAGCTC TGTATCTGGN GGACCCGTGG TGGAACTGAC 841 GAGTTCTGAA CACCCGGNCG CAACCCNGGG NNANGTCCCA NGGNNTTNGG GGGGCCGTTT 901 TTGNGGNCCG ANCNNAGGNA NNGNANTCNA NGNNGAATNC NANCCNNNCN NGNNANGTGG 961 NTCTGGNANG ANANNANAAN CTAAAACNNN NNCCGCCNNN NNCTGNANTT TTTNNNTTNN 1021 GNNTNGNNAN NNNNANNCNN NNNNNNNN //