Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMSCV-GFP-mCentrin2

Retroviral expression vector of mouse Centrin2 fused with EGFP at N-terminal.

Distribution information

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Watanabe, Y. et al. Nat. Commun. 7: 13508, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15401_A7Ikp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F (Pr0574) Region: EGFP 3',insert 5'(Centrin2,PGK pro) Sequence file: RDB15401_A7Ikf.seq
>15401_15401_B2GlF_mVenus-M_F_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTCNAGG TGANNTTNNA GATCCGCCAC ANNCATCGAG    61 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC   121 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC   181 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC   241 ATGGACGAGC TGTACAAGAG ATCTGCCTCT AATTTTAAGA AGACAACCAT GGCATCCAGT   301 GCCCAGAGAA AAAGGATGAG TCCTAAGCCT GAACTTACTG AAGACCAGAA ACAGGAAATC   361 CGGGAAGCTT TTGATCTCTT TGATGCTGAT GGAACTGGAA CTATAGACAT CAAAGAACTG   421 AAGGTGGCAA TGAGGGCCCT GGGCTTTGAG CCCAAGAAAG AAGAAATTAA GAAAATGATA   481 AGTGAAATTG ATAAAGAAGG AACAGGAAAA ATGAACTTCA GTGACTTTTT GACAGTGATG   541 ACTCAGAAAA TGTCTGAGAA AGACACTAAA GAAGAAATCC TGAAAGCTTT CAAGTTGTTC   601 GATGATGATG AGACTGGGAA AATATCATTC AAGAATCTTA AGCGCGTGGC CAAGGAGCTG   661 GGTGAGAACC TGACAGATGA GGAACTGCAG GAAATGATTG ATGAAGCTGA TCGAGATGGA   721 GATGGTGAGG TCAATGAGCA AGAGTTCCTG CGCATCATGA AAAAGACCAG CCTCTATTAA   781 GAATTCTACC GGGTAGGGGA GGCGCTTTTC CCNAGGCAGT CTGGAGCATG CGCTTTANCA   841 GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCNGGC CTCNCACACA TTCCACATCC   901 NCCGGTANGC GCCNNNNGGC TNCGTTNNTT GGNGGNCCCT TCGCGCCACN NNCTACTCCN   961 CCCCNANNCN NGNNNNNCCC CCCNNNCCCN NNNNNNNGNN NNCNNGNAGN NNGNNNCAAA 1021 NNGNNNNNNN CANNNTNNNN NTANNNNNNN NGCNNANNNN NNNNNN //
Primer: PGKpro_F (Pr0249) Region: Zeo,3' LTR Sequence file: RDB15401_A7Ikg.seq
>D04516A1_A7Ik_2_PGKpro_F_E04_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTCTCC GGGCCTTTCG ACCTGCAGCN NNNNCTTATG    61 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG   121 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG   181 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC   241 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC   301 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG   361 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG   421 GAGCAGGACT GAATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT   481 GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG   541 GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA GAGACAGCAG   601 AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA NGGCCAAGAA   661 CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC   721 NAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG   781 CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGANCTCA ATAAAAGANC CCACAACCCC   841 TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCNCCCG GGNACCCGNG TATCNNANAA   901 ACCCTCTTGC ANTTGCATNC NNACTTGNGG TCNCGCTGTT CCTNGGGNNG GTCTNNTCNN 961 ANNGANTGAC TANNNNNNCA NCNGGGGGNN TTTCNTGGNN TNNNANNTTN NNNNNNNNN //
Primer: EGFP-N (Pr0027) Region: gag,MESV_psi Sequence file: RDB15401_A7Ikh.seq
>D04516A1_A7Ik_2_EGFP_N_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNCNNN NCGCNNTNGC TNNCNNTGGN TCTAATTCCG GCGCCTAGAG    61 AAGGAGTGAG GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG   121 GGAGAGACGG GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA   181 CCCAGGGAGG GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG   241 GGTGTCCATG CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT   301 TAAAGGTGCC GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG   361 TAACCCAACG TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT   421 TCCAGTGACC TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCTAA TTTTCAGACA   481 AATACAGAAA CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCGC TGCAGCAGAC   541 AAGACGCGCG GCTTCGGTTC CAAACCGAAA GCAAAAATTC AGACGGAGGC GGGAACTGTT   601 TTAGGTTCTC GTCTCCTACC AGAACCACAT ATCCTGACGG GGTCGGATTC CACATCGACT   661 CCCTTCCTCA GGTCGGGCCA CAAAAACGGC CCCCAAAGTC CCTGGGACGT CTCCCAGGGT   721 TGCGGCCGGG TGTTCAGAAC TCGTCAGTTC CACCACGGGT CCGCCAGATA CAGAGCTAGT   781 TAGCTAACTA GTACAGACGC ANGCGCAAAC ATTAGATGCC GGCNCAAACA CGCACAAAGA   841 CNGAGACAGA CACGAAACGA CCGCTGGCCA GCTTACNNNC CGGCGGGGGG GGTCGGNGGN   901 NCCNNGGCAN GGGNCTNCNA ATCTCNNNGG AACNNNCNAN NGAAANANCC CCNNNNNGGN   961 NANNNNNTCN NTCTGNNNAN ANCNNNCNNN NGNATCANCN NNTCNNCNNN TNNGNNNGNN 1021 NNNNNNNN //
Primer: gag-M_F (Pr0471) Region: gag,EGFP 5' Sequence file: RDB15401_A7Iki.seq
>15401_15401_B2GlF_gag-M_F_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNTNN NNNNNNCTNN NNNNCCGCCT CCTCTTCCTC CNTCCGCCCC    61 GTCTCTCCCC CTTGAACCTC CTCGTTCGAC CCCGCCTCGA TCCTCCCTTT ATCCAGCCCT   121 CACTCCTTCT CTAGGCGCCG GAATTAGATC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC   181 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT   241 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC   301 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA   361 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC   421 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG   481 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA   541 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA   601 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA   661 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG   721 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGANC ACCCAGTCCG CCCTGANCAA   781 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT   841 CACTCTCNGC ATGGACGANC TGTACNAGAG ATCTGCCNCT AATTTTAANA NGANNACCAT   901 GGCATNCANN GNCCAGANAA ANNGNNGANT CCTANGCCTG AANTTANNGN ANANCNNAAA   961 CAGGNAATCN NGNANGNTNN NNTNTCNTTG ATGCTGATNG NANTNGNANN TATNNACNTN 1021 NAAGNNNNNN NNNN //
Primer: pBR322_BamHI_R (Pr0065) Region: 5' LTR,MESV_psi Sequence file: RDB15401_A7Ikj.seq
>D04516A1_A7Ik_2_pBR322_BamHI_R_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGGTCNNG GCTCTAGTTT TGACTCNACN ATATCACCAG    61 CTGAAGCCTA TAGAGTACGA GCCATAGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA   121 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT   181 TGCAAGGCAT GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG   241 AGAGACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC   301 AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA   361 TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC   421 CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG   481 CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT   541 ATTCCCAATA AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG   601 GGTCTCCTCA GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA   661 GATTTGGAGA CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC   721 GGTCGTTTCG TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGNTTGCGC   781 CTGCGTCTGT ACTAGTTAGC TAACTAGCTC TGTATCTGGN GGACCCGTGG TGGAACTGAC   841 GAGTTCTGAA CACCCGGNCG CAACCCNGGG NNANGTCCCA NGGNNTTNGG GGGGCCGTTT   901 TTGNGGNCCG ANCNNAGGNA NNGNANTCNA NGNNGAATNC NANCCNNNCN NGNNANGTGG   961 NTCTGGNANG ANANNANAAN CTAAAACNNN NNCCGCCNNN NNCTGNANTT TTTNNNTTNN 1021 GNNTNGNNAN NNNNANNCNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl