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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-Myc-mG alpha o1

Expression vector of mouse heterotrimeric G-protein alpha-subunit, G(o1)-alpha, myc-tag.

Catalog number RDB15371
Resource name pCAG-Myc-mG alpha o1
Clone info. Expression vector of mouse heterotrimeric G-protein alpha-subunit, G(o1)-alpha, tagged with Myc at N-terminal, CAG promoter.
Comment The CMV promoter region of pcDNA3 (Clontech) was replaced with the promoter/enhancer region of pCAGGS (provided by Dr J.Miyazaki) to generate pcCAG. Expression was confirmed by the depositor with Western blotting.
Vector backbone pCAG-Myc (plasmid)
Selectable markers Amp^r (E. coli) Neo^r (mammalian cells).
Gene/insert name mouse Gnao1 cDNA
Depositor|Developer Nagata, Koh-ichi |
Other clones in our bank
External Database
mouse Gnao1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15371 pCAG-Myc-mG alpha o1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15371_A7I5p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V3
          Sequence file: RDB15371_A7I5a.seq check
          >D04868A4-2_A7I5_pCAG-F_V3_B02_05_ABI24.ab1
              1 CGGGGGGGCG GGCGCTTCGG GGGGGGAGGG GCAGGGCGGG GTTCGGCTTC TGGCGTGTGA
             61 CCGGCGGCTC TAGAGCCTCT GCTAACCATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC
            121 TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTT AATACGACTC
            181 ACTATAGGGA GACCCAAGCT AATCGATATG GAGCAGAAGC TGATCTCCGA GGAGGACCTG
            241 ATATCAAGCT TTGGATCTGG ATGTACGCTG AGCGCAGAGG AGAGAGCCGC CCTCGAGCGG
            301 AGCAAGGCGA TTGAGAAAAA CCTCAAAGAA GATGGCATCA GCGCCGCCAA AGACGTGAAA
            361 TTACTCCTGC TGGGGGCTGG AGAATCAGGA AAAAGCACCA TTGTGAAGCA GATGAAGATC
            421 ATCCATGAAG ATGGCTTCTC TGGGGAAGAC GTGAAGCAGT ACAAGCCTGT GGTCTACAGC
            481 AACACCATCC AGTCTCTGGC GGCCATTGTC CGGGCCATGG ACACTTTGGG CGTGGAGTAT
            541 GGTGACAAGG AGAGGAAGAC GGACTCCAAG ATGGTGTGTG ACGTGGTGAG TCGTATGGAA
            601 GACACTGAAC CGTTCTCTGC AGAACTTCTT TCTGCCATGA TGCGACTCTG GGGCGACTCG
            661 GGGATCCAGG AGTGCTTCAA CCGATCTCGG GAGTATCAGC TCAATGACTC TGCCAAATAC
            721 TACCTGGACA GCCTGGATCG GATTGGAGCC GGTGACTACC AGCCCACTGA GCAGGACATC
            781 CTCCGAACCA GAGTCAAAAC AACTGGCATC GTAGAAACCC ACTTCACCTT CAAGAACCTC
            841 CACTTCAGGC TGTTTGACGT CGGGGGCCAG CGATCTGAAC GCAAGAAGTG ATCCACTGCT
            901 TTGAGGATGT CACGGGCCAT CATCTTCTGT GTCGCACTCA GCGGCTATGA CCAGGTGCTC
            961 CACGAGGACG AAACCACGAA ACCGCATGCA CGAGTCTCTC ATGCTCTTCG ACTCCATCTG
           1021 TAACACAAGG TTTTTCATTG ATACTCAATC ATTCTCCTTC TCACCAGAAG AACCTCTTTG
           1081 GCGAGAAGAT AGAAGTCATC TTGACCATCT GCCTTTCCGA AATACCCAGG CTTCCGTACA
          1141 CCCTAATG
          //
          Primer: pCA-bgter
          Sequence file: RDB15371_A7I5b.seq check
          >D04868A4-2_A715_ pCA-bgter_E03_15_ABI24.ab1
              1 AGCCAGAAGT CCCATAATTT TTGGCAGAGG GAAAAGATCT CAGTGGTATT TGTGAGCCAG
             61 GGCATTGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG GAGTGAATTC
            121 ATACGATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT GCATGCTCGA GGTTTAAACG
            181 CGGCCGCGGT ACCGCTAGAT CAGTACAAGC CGCAGCCCCG GAGATTGTTG GCAATGATGA
            241 TGTCGGTGAC GGCGTCGAAT ACCACCTGGA TATTATTCGT GTCTGTGGCA CAAGTCATGT
            301 GACAGTAAAT TTCTTTGTTG GGTGAGCGGT TTTTGCTTTC AAACTGTGTT TGGATGTAGG
            361 CAGCTGCATC TTCATAGGTG TTGGAGCCTG GGTATTCGGG AAAGCAGATG GTCAAGGGTG
            421 ACTTCTTAAT CTTCTCGCCA AAGAGGTCTT TCTTGTTGAG GAAGAGGATG ATGGAGGTAT
            481 CAATGAAAAA CTTGTTGTTA CAGATGGAGT CGAAGAGCAT GAGAGACTCG TGCATGCGGT
            541 TCGTGGTTTC GTCCTCGTGG AGCACCTGGT CATAGCCGCT GAGTGCGACA CAGAAGATGA
            601 TGGCCGTGAC ATCCTCAAAG CAGTGGATCC ACTTCTTGCG TTCAGATCGC TGGCCCCCGA
            661 CGTCAAACAG CCTGAAGTGG AGGTTCTTGA AGGTGAAGTG GGTTTCTACG ATGCCAGTTG
            721 TTTTGACTCT GGTTCGGAGG ATGTCCTGCT CAGTGGGCTG GTAGTCACCG GCTCCAATCC
            781 GATCCAGGCT GTCCAGGTAG TATTTGGCAG AGTCATTGAG CTGATACTCC CGAGATCGGT
            841 TGAAGCACTC CTGGATCCCC GAGTCGCCCC AGAGTCGCAT CATGGCAGAA AGAAGTTCTG
            901 CAGAGAACGG TTCAGTGTCT TCCATACGAC TCACCACGTC ACACACCATC TTGGAGTCCG
            961 TCTTCCTCTC CTTGTCACCA TACTCCACGC CCAAAGTGTC CATGGGCCCG GGACAATGGG
           1021 CCGCCAGAGA CTGGATGGGT GTTGCTGTAG ACCACAGGCT TGTACTGCTT TCACGTCTTC
           1081 CCCAGAGAAG CCATCTTCAT GATGATCGTC ATCTGCCTCA CAATTGATGC TTTTTTCCAT
          1141 GAATTCCTTC CAAG
          //
          Primer: SV40pro_F
          Sequence file: RDB15371_A7I5c.seq check
          >D04868A4-2_A7I5_SV40pro_F_D02_11_ABI24.ab1
              1 CCTGGGCCTG TGGCCAATCA GGAAGTAGGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
             61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG
            121 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
            181 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
            241 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
            301 TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
            361 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
            421 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
            481 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
            541 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
            601 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC
            661 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
            721 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
            781 GCTATCAGGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG
            841 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC
            901 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GGTTCGAAAT GACCGACCAA
            961 GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCC GCCGCCTTTC TATGAAAGGT
           1021 TGGGCTTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCAGCGC GGGGGATCTT
           1081 CATGCTGAGT TCTTCGCCAA CCCCAACTGG TTATGCAGCT TATATGGGTT ACAAAATAAA
          1141 GGCCAAATAG GCCATTCTAC A
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl