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pCAG-Myc-mG alpha o1 (#RDB15371)

Expression vector of mouse heterotrimeric G-protein alpha-subunit, G(o1)-alpha, myc-tag.

Clone info. Expression vector of mouse heterotrimeric G-protein alpha-subunit, G(o1)-alpha, tagged with Myc at N-terminal, CAG promoter.
Comment The CMV promoter region of pcDNA3 (Clontech) was replaced with the promoter/enhancer region of pCAGGS (provided by Dr J.Miyazaki) to generate pcCAG. Expression was confirmed by the depositor with Western blotting.
Vector backbone pCAG-Myc (plasmid)
Selectable markers Amp^r (E. coli) Neo^r (mammalian cells).
Gene/insert name mouse Gnao1 cDNA
Depositor|Developer Nagata, Koh-ichi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15371 pCAG-Myc-mG alpha o1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG-Myc-mG alpha o1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15371).

Reference section:

Hamada, N., Negishi, Y., Mizuno, M., Miya, F., Hattori, A., Okamoto, N., Kato, M., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Tabata, H., Saitoh, S., Nagata, K.I., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15371_A7I5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 ()
Sequence file: RDB15371_A7I5a.seq check
>D04868A4-2_A7I5_pCAG-F_V3_B02_05_ABI24.ab1
    1 CGGGGGGGCG GGCGCTTCGG GGGGGGAGGG GCAGGGCGGG GTTCGGCTTC TGGCGTGTGA
   61 CCGGCGGCTC TAGAGCCTCT GCTAACCATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC
  121 TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTT AATACGACTC
  181 ACTATAGGGA GACCCAAGCT AATCGATATG GAGCAGAAGC TGATCTCCGA GGAGGACCTG
  241 ATATCAAGCT TTGGATCTGG ATGTACGCTG AGCGCAGAGG AGAGAGCCGC CCTCGAGCGG
  301 AGCAAGGCGA TTGAGAAAAA CCTCAAAGAA GATGGCATCA GCGCCGCCAA AGACGTGAAA
  361 TTACTCCTGC TGGGGGCTGG AGAATCAGGA AAAAGCACCA TTGTGAAGCA GATGAAGATC
  421 ATCCATGAAG ATGGCTTCTC TGGGGAAGAC GTGAAGCAGT ACAAGCCTGT GGTCTACAGC
  481 AACACCATCC AGTCTCTGGC GGCCATTGTC CGGGCCATGG ACACTTTGGG CGTGGAGTAT
  541 GGTGACAAGG AGAGGAAGAC GGACTCCAAG ATGGTGTGTG ACGTGGTGAG TCGTATGGAA
  601 GACACTGAAC CGTTCTCTGC AGAACTTCTT TCTGCCATGA TGCGACTCTG GGGCGACTCG
  661 GGGATCCAGG AGTGCTTCAA CCGATCTCGG GAGTATCAGC TCAATGACTC TGCCAAATAC
  721 TACCTGGACA GCCTGGATCG GATTGGAGCC GGTGACTACC AGCCCACTGA GCAGGACATC
  781 CTCCGAACCA GAGTCAAAAC AACTGGCATC GTAGAAACCC ACTTCACCTT CAAGAACCTC
  841 CACTTCAGGC TGTTTGACGT CGGGGGCCAG CGATCTGAAC GCAAGAAGTG ATCCACTGCT
  901 TTGAGGATGT CACGGGCCAT CATCTTCTGT GTCGCACTCA GCGGCTATGA CCAGGTGCTC
  961 CACGAGGACG AAACCACGAA ACCGCATGCA CGAGTCTCTC ATGCTCTTCG ACTCCATCTG
 1021 TAACACAAGG TTTTTCATTG ATACTCAATC ATTCTCCTTC TCACCAGAAG AACCTCTTTG
 1081 GCGAGAAGAT AGAAGTCATC TTGACCATCT GCCTTTCCGA AATACCCAGG CTTCCGTACA
1141 CCCTAATG
//
Primer: pCA-bgter ()
Sequence file: RDB15371_A7I5b.seq check
>D04868A4-2_A715_ pCA-bgter_E03_15_ABI24.ab1
    1 AGCCAGAAGT CCCATAATTT TTGGCAGAGG GAAAAGATCT CAGTGGTATT TGTGAGCCAG
   61 GGCATTGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG GAGTGAATTC
  121 ATACGATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT GCATGCTCGA GGTTTAAACG
  181 CGGCCGCGGT ACCGCTAGAT CAGTACAAGC CGCAGCCCCG GAGATTGTTG GCAATGATGA
  241 TGTCGGTGAC GGCGTCGAAT ACCACCTGGA TATTATTCGT GTCTGTGGCA CAAGTCATGT
  301 GACAGTAAAT TTCTTTGTTG GGTGAGCGGT TTTTGCTTTC AAACTGTGTT TGGATGTAGG
  361 CAGCTGCATC TTCATAGGTG TTGGAGCCTG GGTATTCGGG AAAGCAGATG GTCAAGGGTG
  421 ACTTCTTAAT CTTCTCGCCA AAGAGGTCTT TCTTGTTGAG GAAGAGGATG ATGGAGGTAT
  481 CAATGAAAAA CTTGTTGTTA CAGATGGAGT CGAAGAGCAT GAGAGACTCG TGCATGCGGT
  541 TCGTGGTTTC GTCCTCGTGG AGCACCTGGT CATAGCCGCT GAGTGCGACA CAGAAGATGA
  601 TGGCCGTGAC ATCCTCAAAG CAGTGGATCC ACTTCTTGCG TTCAGATCGC TGGCCCCCGA
  661 CGTCAAACAG CCTGAAGTGG AGGTTCTTGA AGGTGAAGTG GGTTTCTACG ATGCCAGTTG
  721 TTTTGACTCT GGTTCGGAGG ATGTCCTGCT CAGTGGGCTG GTAGTCACCG GCTCCAATCC
  781 GATCCAGGCT GTCCAGGTAG TATTTGGCAG AGTCATTGAG CTGATACTCC CGAGATCGGT
  841 TGAAGCACTC CTGGATCCCC GAGTCGCCCC AGAGTCGCAT CATGGCAGAA AGAAGTTCTG
  901 CAGAGAACGG TTCAGTGTCT TCCATACGAC TCACCACGTC ACACACCATC TTGGAGTCCG
  961 TCTTCCTCTC CTTGTCACCA TACTCCACGC CCAAAGTGTC CATGGGCCCG GGACAATGGG
 1021 CCGCCAGAGA CTGGATGGGT GTTGCTGTAG ACCACAGGCT TGTACTGCTT TCACGTCTTC
 1081 CCCAGAGAAG CCATCTTCAT GATGATCGTC ATCTGCCTCA CAATTGATGC TTTTTTCCAT
1141 GAATTCCTTC CAAG
//
Primer: SV40pro_F ()
Sequence file: RDB15371_A7I5c.seq check
>D04868A4-2_A7I5_SV40pro_F_D02_11_ABI24.ab1
    1 CCTGGGCCTG TGGCCAATCA GGAAGTAGGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG
  121 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
  181 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
  241 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
  301 TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
  361 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
  421 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
  481 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
  541 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
  601 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC
  661 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
  721 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
  781 GCTATCAGGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG
  841 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC
  901 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GGTTCGAAAT GACCGACCAA
  961 GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCC GCCGCCTTTC TATGAAAGGT
 1021 TGGGCTTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCAGCGC GGGGGATCTT
 1081 CATGCTGAGT TCTTCGCCAA CCCCAACTGG TTATGCAGCT TATATGGGTT ACAAAATAAA
1141 GGCCAAATAG GCCATTCTAC A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898. [link to RRC of NBRP]