Resource data sheet
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pCAG-Myc-mG alpha o1

Expression vector of mouse heterotrimeric G-protein alpha-subunit, G(o1)-alpha, myc-tag.

Catalog number RDB15371
Resource name pCAG-Myc-mG alpha o1
Clone info. Expression vector of mouse heterotrimeric G-protein alpha-subunit, G(o1)-alpha, tagged with Myc at N-terminal, CAG promoter.
The CMV promoter region of pcDNA3 (Clontech) was replaced with the promoter/enhancer region of pCAGGS (provided by Dr J.Miyazaki) to generate pcCAG. Expression was confirmed by the depositor with Western blotting.
Vector backbone pCAG-Myc (plasmid)
Selectable markers Amp^r (E. coli) Neo^r (mammalian cells).
Gene/insert name mouse Gnao1 cDNA
Depositor Nagata, Koh-ichi |
 
Other clones in our bank mouse Gnao1 (NCBI Gene 14681) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)
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RDB15371 pCAG-Myc-mG alpha o1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15371_A7I5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 RDB15371_A7I5a.seq
>D04868A4-2_A7I5_pCAG-F_V3_B02_05_ABI24.ab1
    1 CGGGGGGGCG GGCGCTTCGG GGGGGGAGGG GCAGGGCGGG GTTCGGCTTC TGGCGTGTGA
   61 CCGGCGGCTC TAGAGCCTCT GCTAACCATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC
  121 TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTT AATACGACTC
  181 ACTATAGGGA GACCCAAGCT AATCGATATG GAGCAGAAGC TGATCTCCGA GGAGGACCTG
  241 ATATCAAGCT TTGGATCTGG ATGTACGCTG AGCGCAGAGG AGAGAGCCGC CCTCGAGCGG
  301 AGCAAGGCGA TTGAGAAAAA CCTCAAAGAA GATGGCATCA GCGCCGCCAA AGACGTGAAA
  361 TTACTCCTGC TGGGGGCTGG AGAATCAGGA AAAAGCACCA TTGTGAAGCA GATGAAGATC
  421 ATCCATGAAG ATGGCTTCTC TGGGGAAGAC GTGAAGCAGT ACAAGCCTGT GGTCTACAGC
  481 AACACCATCC AGTCTCTGGC GGCCATTGTC CGGGCCATGG ACACTTTGGG CGTGGAGTAT
  541 GGTGACAAGG AGAGGAAGAC GGACTCCAAG ATGGTGTGTG ACGTGGTGAG TCGTATGGAA
  601 GACACTGAAC CGTTCTCTGC AGAACTTCTT TCTGCCATGA TGCGACTCTG GGGCGACTCG
  661 GGGATCCAGG AGTGCTTCAA CCGATCTCGG GAGTATCAGC TCAATGACTC TGCCAAATAC
  721 TACCTGGACA GCCTGGATCG GATTGGAGCC GGTGACTACC AGCCCACTGA GCAGGACATC
  781 CTCCGAACCA GAGTCAAAAC AACTGGCATC GTAGAAACCC ACTTCACCTT CAAGAACCTC
  841 CACTTCAGGC TGTTTGACGT CGGGGGCCAG CGATCTGAAC GCAAGAAGTG ATCCACTGCT
  901 TTGAGGATGT CACGGGCCAT CATCTTCTGT GTCGCACTCA GCGGCTATGA CCAGGTGCTC
  961 CACGAGGACG AAACCACGAA ACCGCATGCA CGAGTCTCTC ATGCTCTTCG ACTCCATCTG
 1021 TAACACAAGG TTTTTCATTG ATACTCAATC ATTCTCCTTC TCACCAGAAG AACCTCTTTG
 1081 GCGAGAAGAT AGAAGTCATC TTGACCATCT GCCTTTCCGA AATACCCAGG CTTCCGTACA
1141 CCCTAATG
//
Primer: pCA-bgter RDB15371_A7I5b.seq
>D04868A4-2_A715_ pCA-bgter_E03_15_ABI24.ab1
    1 AGCCAGAAGT CCCATAATTT TTGGCAGAGG GAAAAGATCT CAGTGGTATT TGTGAGCCAG
   61 GGCATTGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG GAGTGAATTC
  121 ATACGATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT GCATGCTCGA GGTTTAAACG
  181 CGGCCGCGGT ACCGCTAGAT CAGTACAAGC CGCAGCCCCG GAGATTGTTG GCAATGATGA
  241 TGTCGGTGAC GGCGTCGAAT ACCACCTGGA TATTATTCGT GTCTGTGGCA CAAGTCATGT
  301 GACAGTAAAT TTCTTTGTTG GGTGAGCGGT TTTTGCTTTC AAACTGTGTT TGGATGTAGG
  361 CAGCTGCATC TTCATAGGTG TTGGAGCCTG GGTATTCGGG AAAGCAGATG GTCAAGGGTG
  421 ACTTCTTAAT CTTCTCGCCA AAGAGGTCTT TCTTGTTGAG GAAGAGGATG ATGGAGGTAT
  481 CAATGAAAAA CTTGTTGTTA CAGATGGAGT CGAAGAGCAT GAGAGACTCG TGCATGCGGT
  541 TCGTGGTTTC GTCCTCGTGG AGCACCTGGT CATAGCCGCT GAGTGCGACA CAGAAGATGA
  601 TGGCCGTGAC ATCCTCAAAG CAGTGGATCC ACTTCTTGCG TTCAGATCGC TGGCCCCCGA
  661 CGTCAAACAG CCTGAAGTGG AGGTTCTTGA AGGTGAAGTG GGTTTCTACG ATGCCAGTTG
  721 TTTTGACTCT GGTTCGGAGG ATGTCCTGCT CAGTGGGCTG GTAGTCACCG GCTCCAATCC
  781 GATCCAGGCT GTCCAGGTAG TATTTGGCAG AGTCATTGAG CTGATACTCC CGAGATCGGT
  841 TGAAGCACTC CTGGATCCCC GAGTCGCCCC AGAGTCGCAT CATGGCAGAA AGAAGTTCTG
  901 CAGAGAACGG TTCAGTGTCT TCCATACGAC TCACCACGTC ACACACCATC TTGGAGTCCG
  961 TCTTCCTCTC CTTGTCACCA TACTCCACGC CCAAAGTGTC CATGGGCCCG GGACAATGGG
 1021 CCGCCAGAGA CTGGATGGGT GTTGCTGTAG ACCACAGGCT TGTACTGCTT TCACGTCTTC
 1081 CCCAGAGAAG CCATCTTCAT GATGATCGTC ATCTGCCTCA CAATTGATGC TTTTTTCCAT
1141 GAATTCCTTC CAAG
//
Primer: SV40pro_F RDB15371_A7I5c.seq
>D04868A4-2_A7I5_SV40pro_F_D02_11_ABI24.ab1
    1 CCTGGGCCTG TGGCCAATCA GGAAGTAGGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG
  121 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
  181 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
  241 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
  301 TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
  361 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
  421 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
  481 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
  541 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
  601 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC
  661 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
  721 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
  781 GCTATCAGGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG
  841 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC
  901 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GGTTCGAAAT GACCGACCAA
  961 GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCC GCCGCCTTTC TATGAAAGGT
 1021 TGGGCTTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCAGCGC GGGGGATCTT
 1081 CATGCTGAGT TCTTCGCCAA CCCCAACTGG TTATGCAGCT TATATGGGTT ACAAAATAAA
1141 GGCCAAATAG GCCATTCTAC A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

2019.02.24

GNP_filter3_RDBDEP_html_190131.pl