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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-Myc-mG alpha i3 (#RDB15370)

Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i3, myc-tag.

Clone info. Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i3, tagged with Myc at N-terminal, CAG promoter.
Comment The CMV promoter region of pcDNA3 (Clontech) was replaced with the promoter/enhancer region of pCAGGS (provided by Dr J.Miyazaki) to generate pcCAG. Expression was confirmed by the depositor with Western blotting.
Vector backbone pCAG-Myc (plasmid)
Selectable markers Amp^r (E. coli) Neo^r (mammalian cells).
Gene/insert name mouse Gnai3 cDNA
Depositor|Developer Nagata, Koh-ichi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15370 pCAG-Myc-mG alpha i3 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG-Myc-mG alpha i3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15370).

Reference section:

Hamada, N., Negishi, Y., Mizuno, M., Miya, F., Hattori, A., Okamoto, N., Kato, M., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Tabata, H., Saitoh, S., Nagata, K.I., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15370_A7I5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 ()
Sequence file: RDB15370_A7I5a.seq check
>D04868A3-1_A715_ pCAG-F_V3_F03_18_ABI24.ab1
    1 CCTTGGGGGC AGGCTGCTTC GGGGGGGGAG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT
   61 GACCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT
  121 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TTAATACGAC
  181 TCACTATAGG GAGACCCAAG CTAATCGATA TGGAGCAGAA GCTGATCTCC GAGGAGGACC
  241 TGATATCAAG CTTTGGATCC GGCTGCACGT TGAGCGCCGA GGACAAGGCG GCGGTGGAGC
  301 GGAGCAAGAT GATCGACCGC AACTTGCGGG AGGACGGGGA GAAAGCGGCC AAAGAAGTGA
  361 AGCTGCTGCT GCTCGGCGCT GGAGAATCTG GTAAAAGTAC CATCGTGAAA CAGATGAAAA
  421 TCATTCATGA GGACGGCTAT TCAGAGGACG AATGTAAACA GTATAAAGTA GTTGTCTACA
  481 GCAATACTAT TCAGTCCATC ATTGCAATCA TACGAGCCAT GGGACGGTTG AAGATTGATT
  541 TTGGGGAATC TGCCAGAGCA GATGATGCCC GACAGTTATT TGTTTTAGCT GGGAGTGCTG
  601 AAGAAGGAGT CATGACTTCA GAACTAGCAG GCGTGATTAA ACGTTTATGG CGAGATGGCG
  661 GGGTACAGGC ATGCTTTAGC AGGTCCAGGG AATATCAGCT CAATGATTCT GCTTCATACT
  721 ACCTAAATGA TTTGGATAGA ATATCCCAGA CCAACTACAT TCCAACTCAG CAAGATGTTC
  781 TTCGGACAAG AGTGAAGACT ACAGGCATTG TGGAGACCCA CTTCACCTTC AAGGAACTCT
  841 ACTTCAAAAT GTTTGATGTA GGTGGCCAAA GATCCGAACG AAAAAAAGTG GATTCACTGT
  901 TTTGAGGGAG TGACAGCAAT TATCTTTTGT GTGGCTCTCA GTGATTACGA CCTTGTTCTG
  961 GGCTGAAGGA TGAGGAAATG AAACCGAAAT GCATGAAAGC ATGAAATTGT TTGACAGCAT
 1021 TTGTAACAAC AAATGGTTTT ACAGACACTT CATCATCTCT TCTTTATTAG AAGACCTTTT
1081 TGAGGAAAAA ATAGAGGAGT TCAATAACAA TCTGTTATCC AGGAAATACC CACTAAGGT
//
Primer: pCA-bgter ()
Sequence file: RDB15370_A7I5b.seq check
>D04868A3-1_A715_ pCA-bgter_C03_09_ABI24.ab1
    1 AGCTTAGAAA AGTCCAATAA TTTTTGGGCA GAGGGAAAAA GATCTCAGTG GTATTTGTGA
   61 GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG
  121 AATTCATACG ATTTAGGTGA CACTATAGAA TAGGGCCCTC TAGATGCATG CTCGAGGTTT
  181 AAACGCGGCC GCGGTACCGC TAGATCAATA AAGCCCACAT TCCTTTAAGT TGTTTTTAAT
  241 GATGACATCC GTAACAGCAT CAAAAACAAA CTGCACATTT TTGGTGTCTG TGGCACAGGT
  301 GAAGTGAGTG TAGACCTCCT TGGTATCTTT TCTTCGGTTC AGATCTTCAA ACTGGCACTG
  361 AATGTAAGCA GCTGCCTCTT CGTATGTATT GGAACCTGTG TATTCTGGAT AACAGATTGT
  421 TAATGGACTC CTCTTTATTT TTTCCTCAAA AAGGTCTTTC TTATTAAGAA AGAGAATGAT
  481 TGAAGTGTCT GTAAACCATT TGTTGTTACA AATGCTGTCA AACAATTTCA TGCTTTCATG
  541 CATTCGGTTC ATTTCCTCAT CCTCAGCCAG AACAAGGTCG TAATCACTGA GAGCCACACA
  601 AAAGATAATT GCTGTCACTC CCTCAAAACA GTGAATCCAC TTTTTTCGTT CGGATCTTTG
  661 GCCACCTACA TCAAACATTT TGAAGTAGAG TTCCTTGAAG GTGAAGTGGG TCTCCACAAT
  721 GCCTGTAGTC TTCACTCTTG TCCGAAGAAC ATCTTGCTGA GTTGGAATGT AGTTGGTCTG
  781 GGATATTCTA TCCAAATCAT TTAGGTAGTA TGAAGCAGAA TCATTGAGCT GATATTCCCT
  841 GGACCTGCTA AAGCATGCCT GTACCCCGCC ATCTCGCCAT AAACGTTTAA TCACGCCTGC
  901 TAGTTCTGAA GTCATGACTC CTTCTTCAGC ACTCCCAGCT AAAACAAATA AACTGTCGGG
  961 CATCATCTGC TCTGGCAGAT TCCCCAAAAT CAAATCTTTC AACCGTCCCA TGGCTCGTAT
 1021 GATTGCCAAT GATGGACTGA AATAGTATTG CTGGTAGACA ACTACTTTTA TACCTGTTTA
 1081 CAATTCGTCC TCTGAATAGG CCGGTCCCTC ATGATGATAT TCCATCTGAT TTCAGCGATT
1141 GGGTAACCCT TTTTACCAGG ATTTCCTTCC CCAGACG
//
Primer: SV40pro_F ()
Sequence file: RDB15370_A7I5c.seq check
>D04868A3-1_A7I5_SV40pro_F_A02_02_ABI24.ab1
    1 GCTTGGGCCC GGGGCATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA
  961 CGCCCAACCT GCCCATCACG AGATTTCGAT TTCCACCGCC GCCTTCTATG AAAGGTGGGC
 1021 TTCGGATCGT TTCCGGACGC GGCTGATGAT CTCAGCGCGG GATCTCATGC TGGAGGTCTC
1081 GTCACCGAAC TTGGTATGCA GCTTATATGG TACAATAAAG CCGAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898. [link to RRC of NBRP]