Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i3, myc-tag.
Clone info. | Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i3, tagged with Myc at N-terminal, CAG promoter. |
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Comment | The CMV promoter region of pcDNA3 (Clontech) was replaced with the promoter/enhancer region of pCAGGS (provided by Dr J.Miyazaki) to generate pcCAG. Expression was confirmed by the depositor with Western blotting. |
Vector backbone | pCAG-Myc (plasmid) |
Selectable markers | Amp^r (E. coli) Neo^r (mammalian cells). |
Gene/insert name | mouse Gnai3 cDNA |
Depositor|Developer | Nagata, Koh-ichi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017) Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)。 MTAに書く付加的使用条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15370 | pCAG-Myc-mG alpha i3 | DNA solution |
Materials & Methods section:
The pCAG-Myc-mG alpha i3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15370). |
Reference section:
Hamada, N., Negishi, Y., Mizuno, M., Miya, F., Hattori, A., Okamoto, N., Kato, M., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Tabata, H., Saitoh, S., Nagata, K.I., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15370_A7I5p1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pCAG-F_V3 () Sequence file: RDB15370_A7I5a.seq ![]() |
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>D04868A3-1_A715_ pCAG-F_V3_F03_18_ABI24.ab1 1 CCTTGGGGGC AGGCTGCTTC GGGGGGGGAG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT 61 GACCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT 121 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TTAATACGAC 181 TCACTATAGG GAGACCCAAG CTAATCGATA TGGAGCAGAA GCTGATCTCC GAGGAGGACC 241 TGATATCAAG CTTTGGATCC GGCTGCACGT TGAGCGCCGA GGACAAGGCG GCGGTGGAGC 301 GGAGCAAGAT GATCGACCGC AACTTGCGGG AGGACGGGGA GAAAGCGGCC AAAGAAGTGA 361 AGCTGCTGCT GCTCGGCGCT GGAGAATCTG GTAAAAGTAC CATCGTGAAA CAGATGAAAA 421 TCATTCATGA GGACGGCTAT TCAGAGGACG AATGTAAACA GTATAAAGTA GTTGTCTACA 481 GCAATACTAT TCAGTCCATC ATTGCAATCA TACGAGCCAT GGGACGGTTG AAGATTGATT 541 TTGGGGAATC TGCCAGAGCA GATGATGCCC GACAGTTATT TGTTTTAGCT GGGAGTGCTG 601 AAGAAGGAGT CATGACTTCA GAACTAGCAG GCGTGATTAA ACGTTTATGG CGAGATGGCG 661 GGGTACAGGC ATGCTTTAGC AGGTCCAGGG AATATCAGCT CAATGATTCT GCTTCATACT 721 ACCTAAATGA TTTGGATAGA ATATCCCAGA CCAACTACAT TCCAACTCAG CAAGATGTTC 781 TTCGGACAAG AGTGAAGACT ACAGGCATTG TGGAGACCCA CTTCACCTTC AAGGAACTCT 841 ACTTCAAAAT GTTTGATGTA GGTGGCCAAA GATCCGAACG AAAAAAAGTG GATTCACTGT 901 TTTGAGGGAG TGACAGCAAT TATCTTTTGT GTGGCTCTCA GTGATTACGA CCTTGTTCTG 961 GGCTGAAGGA TGAGGAAATG AAACCGAAAT GCATGAAAGC ATGAAATTGT TTGACAGCAT 1021 TTGTAACAAC AAATGGTTTT ACAGACACTT CATCATCTCT TCTTTATTAG AAGACCTTTT 1081 TGAGGAAAAA ATAGAGGAGT TCAATAACAA TCTGTTATCC AGGAAATACC CACTAAGGT // |
Primer: pCA-bgter () Sequence file: RDB15370_A7I5b.seq ![]() |
>D04868A3-1_A715_ pCA-bgter_C03_09_ABI24.ab1 1 AGCTTAGAAA AGTCCAATAA TTTTTGGGCA GAGGGAAAAA GATCTCAGTG GTATTTGTGA 61 GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTG 121 AATTCATACG ATTTAGGTGA CACTATAGAA TAGGGCCCTC TAGATGCATG CTCGAGGTTT 181 AAACGCGGCC GCGGTACCGC TAGATCAATA AAGCCCACAT TCCTTTAAGT TGTTTTTAAT 241 GATGACATCC GTAACAGCAT CAAAAACAAA CTGCACATTT TTGGTGTCTG TGGCACAGGT 301 GAAGTGAGTG TAGACCTCCT TGGTATCTTT TCTTCGGTTC AGATCTTCAA ACTGGCACTG 361 AATGTAAGCA GCTGCCTCTT CGTATGTATT GGAACCTGTG TATTCTGGAT AACAGATTGT 421 TAATGGACTC CTCTTTATTT TTTCCTCAAA AAGGTCTTTC TTATTAAGAA AGAGAATGAT 481 TGAAGTGTCT GTAAACCATT TGTTGTTACA AATGCTGTCA AACAATTTCA TGCTTTCATG 541 CATTCGGTTC ATTTCCTCAT CCTCAGCCAG AACAAGGTCG TAATCACTGA GAGCCACACA 601 AAAGATAATT GCTGTCACTC CCTCAAAACA GTGAATCCAC TTTTTTCGTT CGGATCTTTG 661 GCCACCTACA TCAAACATTT TGAAGTAGAG TTCCTTGAAG GTGAAGTGGG TCTCCACAAT 721 GCCTGTAGTC TTCACTCTTG TCCGAAGAAC ATCTTGCTGA GTTGGAATGT AGTTGGTCTG 781 GGATATTCTA TCCAAATCAT TTAGGTAGTA TGAAGCAGAA TCATTGAGCT GATATTCCCT 841 GGACCTGCTA AAGCATGCCT GTACCCCGCC ATCTCGCCAT AAACGTTTAA TCACGCCTGC 901 TAGTTCTGAA GTCATGACTC CTTCTTCAGC ACTCCCAGCT AAAACAAATA AACTGTCGGG 961 CATCATCTGC TCTGGCAGAT TCCCCAAAAT CAAATCTTTC AACCGTCCCA TGGCTCGTAT 1021 GATTGCCAAT GATGGACTGA AATAGTATTG CTGGTAGACA ACTACTTTTA TACCTGTTTA 1081 CAATTCGTCC TCTGAATAGG CCGGTCCCTC ATGATGATAT TCCATCTGAT TTCAGCGATT 1141 GGGTAACCCT TTTTACCAGG ATTTCCTTCC CCAGACG // |
Primer: SV40pro_F () Sequence file: RDB15370_A7I5c.seq ![]() |
>D04868A3-1_A7I5_SV40pro_F_A02_02_ABI24.ab1 1 GCTTGGGCCC GGGGCATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT 121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC 241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA 541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA 661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG 721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT 781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG 841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC 901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA 961 CGCCCAACCT GCCCATCACG AGATTTCGAT TTCCACCGCC GCCTTCTATG AAAGGTGGGC 1021 TTCGGATCGT TTCCGGACGC GGCTGATGAT CTCAGCGCGG GATCTCATGC TGGAGGTCTC 1081 GTCACCGAAC TTGGTATGCA GCTTATATGG TACAATAAAG CCGAT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898. [link to RRC of NBRP] |
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