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pCAG-Myc-mG alpha i1

Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i1, myc-tag.

Catalog number RDB15369
Resource name pCAG-Myc-mG alpha i1
Clone info. Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i1, tagged with Myc at N-terminal, CAG promoter.
The CMV promoter region of pcDNA3 (Clontech) was replaced with the promoter/enhancer region of pCAGGS (provided by Dr J.Miyazaki) to generate pcCAG. Expression was confirmed by the depositor with Western blotting.
Vector backbone pCAG-Myc (plasmid)
Selectable markers Amp^r (E. coli) Neo^r (mammalian cells).
Gene/insert name mouse Gnai1 cDNA
Depositor Nagata, Koh-ichi |
 
Other clones in our bank mouse Gnai1 (NCBI Gene 14677) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)
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RDB15369 pCAG-Myc-mG alpha i1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15369_A7I5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 RDB15369_A7I5a.seq
>D04868A2-2_A7I5_pCAG-F_V3_D01_10_ABI24.ab1
    1 TGCGTGGGGG GGCAGCCTGC TTTCGGGGGG GGAGGGGCAG GGCGGGGTTC GGCTTCTGGC
   61 GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC
  121 AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTTAATA
  181 CGACTCACTA TAGGGAGACC CAAGCTAATC GATATGGAGC AGAAGCTGAT CTCCGAGGAG
  241 GACCTGATAT CAAGCTTTGG ATCCGGCGCG CCGGGCTGCA CATTGAGCGC TGAGGACAAG
  301 GCGGCCGTGG AGCGCAGCAA GATGATCGAC CGCAACCTCC GGGAGGACGG CGAGAAGGCG
  361 GCGCGGGAGG TCAAGCTGCT GCTGCTGGGT GCTGGGGAAT CTGGAAAGAG TACCATTGTG
  421 AAGCAGATGA AGATTATCCA CGAAGCCGGC TACTCGGAAG AGGAGTGTAA GCAGTACAAG
  481 GCAGTGGTCT ACAGCAACAC TATCCAGTCC ATCATTGCCA TCATTAGAGC CATGGGGAGG
  541 TTGAAAATCG ACTTCGGAGA CTCTGCTCGG GCGGATGATG CTCGCCAACT TTTCGTGCTT
  601 GCTGGGGCTG CAGAAGAAGG CTTTATGACT GCAGAGCTCG CCGGTGTCAT AAAGAGACTG
  661 TGGAAAGACA GTGGTGTGCA AGCCTGCTTC AACAGATCCC GGGAGTACCA GCTGAACGAT
  721 TCGGCAGCGT ACTATCTGAA TGACTTGGAC AGAATAGCAC AGCCAAATTA CATCCCAACT
  781 CAGCAGGATG TCCTCAGAAC CAGAGTGAAG ACCACAGGGA TTGTGGAAAC CCACTTTACC
  841 TTCAAAGATC TTCATTTTAA AATGTTTGAC GTGGGAGGTC AGAGGTCAGA GCGGGAAGAA
  901 GTGGATCCAC TGCTTTGAAA GGGGTGACCG CCATCATCTT TCTGTGTGGG CCCTGAGTGA
  961 CTATGACCTG GTTCTTGCTG AAGATGAAAG AAATGAAACC GGTATGCACG AGAGCATGAA
 1021 GCTGTTCGAT AGCATCTGTA ACAACAAGTG GGTTTACAGA CCACGTCCCA TCATCCTTTT
 1081 TCCTCACAAA GAAGGACCTT CTTCGGAAGG AAAAATAAAA GAAGCCTCCT CAACGGATTA
1141 TGCTAACTCT AGGAAATATG TGGCCAGAGG GGCTCTCC
//
Primer: pCA-bgter RDB15369_A7I5b.seq
>D04868A2-2_A715_ pCA-bgter_B03_06_ABI24.ab1
    1 GACGGCGGAA TGTCCCATAA TTTTTGGCAG AGGGAAAAGA TCTCAGTGGT ATTTGTGAGC
   61 CAGGGCATTG GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT GAGGAGTGAA
  121 TTCATACGAT TTAGGTGACA CTATAGAATA GGGCCCTCTA GATGCATGCT CGAGGTTTAA
  181 ACTTAGAAGA GACCACAGTC TTTTAGGTTA TTCTTTATGA TGACGTCTGT TACAGCATCG
  241 AACACGAACT GCACGTTCTT CGTATCTGTG GCGCACGTGA AGTGGGTGTA AATTTCCTTT
  301 GTGTCCTTCC TTTTATTGAG GTCTTCAAAC TGACACTGAA TATACGCGGC CGCTTCTTCA
  361 TATGTGTTTG AGCCTGCATA TTCTGGGTAG CATATCGTGA GGGGGCTCTT TTTTATTTTT
  421 TCTTCGAAGA GGTCCTTCTT GTTGAGGAAA AGGATGATGG ACGTGTCTGT AAACCACTTG
  481 TTGTTACAGA TGCTATCGAA CAGCTTCATG CTCTCGTGCA TACGGTTCAT TTCTTCATCT
  541 TCAGCAAGAA CCAGGTCATA GTCACTCAGG GCCACACAGA AGATGATGGC GGTCACCCCT
  601 TCAAAGCAGT GGATCCACTT CTTCCGCTCT GACCTCTGAC CTCCCACGTC AAACATTTTA
  661 AAATGAAGAT CTTTGAAGGT AAAGTGGGTT TCCACAATCC CTGTGGTCTT CACTCTGGTT
  721 CTGAGGACAT CCTGCTGAGT TGGGATGTAA TTTGGCTGTG CTATTCTGTC CAAGTCATTC
  781 AGATAGTACG CTGCCGAATC GTTCAGCTGG TACTCCCGGG ATCTGTTGAA GCAGGCTTGC
  841 ACACCACTGT CTTTCCACAG TCTCTTTATG ACACCGGCGA GCTCTGCAGT CATAAAGCCT
  901 TCTTCTGCAG CCCCAGCAAG CACGAAAAGT TGGCGAGCAT CATCCGCCCG AGCAGAGTCT
  961 CCGAAGTCGA TTTTCAACCT CCCCATGGGC TCTAATGATG GCAATGATGG GACTGGGATA
 1021 GTGTTGCTGT AGACCACTGC CCTTGTACTG CTTACACTCG TCTTCCCGAG TAGCCGGGCT
 1081 TTCGTGGATA ATCTTCATCT GCTTCACATT GTAACTCTTT CAGATTTCCC CAAGCCACCC
1141 AGCAGCAGGC AAGCCATTGA ACT
//
Primer: SV40pro_F RDB15369_A7I5c.seq
>D04868A2-2_A7I5_SV40pro_F_F01_16_ABI24.ab1
    1 CCTGGGCCTG GGGCATTCAA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC
  961 GCCCAAACCT GCCATCACGA GATTCGATTC CACGCCGCCT TCTATGAAGT GGGGCTTCGG
 1021 AATCGTTTCC GGACGCGCCT GATGATCCTC CAGCGCGGGG AATCTCATGG CTGGAGTCCT
1081 CGCCACCGAA CTGTTAATGC AGCTATATGG TACATAGGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

2019.02.24

GNP_filter3_RDBDEP_html_190131.pl