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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-Myc-mG alpha i1

Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i1, myc-tag.

Catalog number RDB15369
Resource name pCAG-Myc-mG alpha i1
Clone info. Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i1, tagged with Myc at N-terminal, CAG promoter.
Comment The CMV promoter region of pcDNA3 (Clontech) was replaced with the promoter/enhancer region of pCAGGS (provided by Dr J.Miyazaki) to generate pcCAG. Expression was confirmed by the depositor with Western blotting.
Vector backbone pCAG-Myc (plasmid)
Selectable markers Amp^r (E. coli) Neo^r (mammalian cells).
Gene/insert name mouse Gnai1 cDNA
Depositor|Developer Nagata, Koh-ichi |
Other clones in our bank
External Database
mouse Gnai1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15369 pCAG-Myc-mG alpha i1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15369_A7I5p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V3
          Sequence file: RDB15369_A7I5a.seq check
          >D04868A2-2_A7I5_pCAG-F_V3_D01_10_ABI24.ab1
              1 TGCGTGGGGG GGCAGCCTGC TTTCGGGGGG GGAGGGGCAG GGCGGGGTTC GGCTTCTGGC
             61 GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC
            121 AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTTAATA
            181 CGACTCACTA TAGGGAGACC CAAGCTAATC GATATGGAGC AGAAGCTGAT CTCCGAGGAG
            241 GACCTGATAT CAAGCTTTGG ATCCGGCGCG CCGGGCTGCA CATTGAGCGC TGAGGACAAG
            301 GCGGCCGTGG AGCGCAGCAA GATGATCGAC CGCAACCTCC GGGAGGACGG CGAGAAGGCG
            361 GCGCGGGAGG TCAAGCTGCT GCTGCTGGGT GCTGGGGAAT CTGGAAAGAG TACCATTGTG
            421 AAGCAGATGA AGATTATCCA CGAAGCCGGC TACTCGGAAG AGGAGTGTAA GCAGTACAAG
            481 GCAGTGGTCT ACAGCAACAC TATCCAGTCC ATCATTGCCA TCATTAGAGC CATGGGGAGG
            541 TTGAAAATCG ACTTCGGAGA CTCTGCTCGG GCGGATGATG CTCGCCAACT TTTCGTGCTT
            601 GCTGGGGCTG CAGAAGAAGG CTTTATGACT GCAGAGCTCG CCGGTGTCAT AAAGAGACTG
            661 TGGAAAGACA GTGGTGTGCA AGCCTGCTTC AACAGATCCC GGGAGTACCA GCTGAACGAT
            721 TCGGCAGCGT ACTATCTGAA TGACTTGGAC AGAATAGCAC AGCCAAATTA CATCCCAACT
            781 CAGCAGGATG TCCTCAGAAC CAGAGTGAAG ACCACAGGGA TTGTGGAAAC CCACTTTACC
            841 TTCAAAGATC TTCATTTTAA AATGTTTGAC GTGGGAGGTC AGAGGTCAGA GCGGGAAGAA
            901 GTGGATCCAC TGCTTTGAAA GGGGTGACCG CCATCATCTT TCTGTGTGGG CCCTGAGTGA
            961 CTATGACCTG GTTCTTGCTG AAGATGAAAG AAATGAAACC GGTATGCACG AGAGCATGAA
           1021 GCTGTTCGAT AGCATCTGTA ACAACAAGTG GGTTTACAGA CCACGTCCCA TCATCCTTTT
           1081 TCCTCACAAA GAAGGACCTT CTTCGGAAGG AAAAATAAAA GAAGCCTCCT CAACGGATTA
          1141 TGCTAACTCT AGGAAATATG TGGCCAGAGG GGCTCTCC
          //
          Primer: pCA-bgter
          Sequence file: RDB15369_A7I5b.seq check
          >D04868A2-2_A715_ pCA-bgter_B03_06_ABI24.ab1
              1 GACGGCGGAA TGTCCCATAA TTTTTGGCAG AGGGAAAAGA TCTCAGTGGT ATTTGTGAGC
             61 CAGGGCATTG GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT GAGGAGTGAA
            121 TTCATACGAT TTAGGTGACA CTATAGAATA GGGCCCTCTA GATGCATGCT CGAGGTTTAA
            181 ACTTAGAAGA GACCACAGTC TTTTAGGTTA TTCTTTATGA TGACGTCTGT TACAGCATCG
            241 AACACGAACT GCACGTTCTT CGTATCTGTG GCGCACGTGA AGTGGGTGTA AATTTCCTTT
            301 GTGTCCTTCC TTTTATTGAG GTCTTCAAAC TGACACTGAA TATACGCGGC CGCTTCTTCA
            361 TATGTGTTTG AGCCTGCATA TTCTGGGTAG CATATCGTGA GGGGGCTCTT TTTTATTTTT
            421 TCTTCGAAGA GGTCCTTCTT GTTGAGGAAA AGGATGATGG ACGTGTCTGT AAACCACTTG
            481 TTGTTACAGA TGCTATCGAA CAGCTTCATG CTCTCGTGCA TACGGTTCAT TTCTTCATCT
            541 TCAGCAAGAA CCAGGTCATA GTCACTCAGG GCCACACAGA AGATGATGGC GGTCACCCCT
            601 TCAAAGCAGT GGATCCACTT CTTCCGCTCT GACCTCTGAC CTCCCACGTC AAACATTTTA
            661 AAATGAAGAT CTTTGAAGGT AAAGTGGGTT TCCACAATCC CTGTGGTCTT CACTCTGGTT
            721 CTGAGGACAT CCTGCTGAGT TGGGATGTAA TTTGGCTGTG CTATTCTGTC CAAGTCATTC
            781 AGATAGTACG CTGCCGAATC GTTCAGCTGG TACTCCCGGG ATCTGTTGAA GCAGGCTTGC
            841 ACACCACTGT CTTTCCACAG TCTCTTTATG ACACCGGCGA GCTCTGCAGT CATAAAGCCT
            901 TCTTCTGCAG CCCCAGCAAG CACGAAAAGT TGGCGAGCAT CATCCGCCCG AGCAGAGTCT
            961 CCGAAGTCGA TTTTCAACCT CCCCATGGGC TCTAATGATG GCAATGATGG GACTGGGATA
           1021 GTGTTGCTGT AGACCACTGC CCTTGTACTG CTTACACTCG TCTTCCCGAG TAGCCGGGCT
           1081 TTCGTGGATA ATCTTCATCT GCTTCACATT GTAACTCTTT CAGATTTCCC CAAGCCACCC
          1141 AGCAGCAGGC AAGCCATTGA ACT
          //
          Primer: SV40pro_F
          Sequence file: RDB15369_A7I5c.seq check
          >D04868A2-2_A7I5_SV40pro_F_F01_16_ABI24.ab1
              1 CCTGGGCCTG GGGCATTCAA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
             61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
            121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
            661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
            781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
            841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
            901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC
            961 GCCCAAACCT GCCATCACGA GATTCGATTC CACGCCGCCT TCTATGAAGT GGGGCTTCGG
           1021 AATCGTTTCC GGACGCGCCT GATGATCCTC CAGCGCGGGG AATCTCATGG CTGGAGTCCT
          1081 CGCCACCGAA CTGTTAATGC AGCTATATGG TACATAGGCA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl