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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-Myc-mG alpha i2 (#RDB15368)

Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i2, myc-tag.

Clone info. Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i2, tagged with Myc at N-terminal, CAG promoter.
Comment The CMV promoter region of pcDNA3 (Clontech) was replaced with the promoter/enhancer region of pCAGGS (provided by Dr J.Miyazaki) to generate pcCAG. Expression was confirmed by the depositor with Western blotting.
Vector backbone pCAG-Myc (plasmid)
Selectable markers Amp^r (E. coli) Neo^r (mammalian cells).
Gene/insert name mouse Gnai2 cDNA
Depositor|Developer Nagata, Koh-ichi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15368 pCAG-Myc-mG alpha i2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG-Myc-mG alpha i2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15368).

Reference section:

Hamada, N., Negishi, Y., Mizuno, M., Miya, F., Hattori, A., Okamoto, N., Kato, M., Tsunoda, T., Yamasaki, M., Kanemura, Y., Kosaki, K., Tabata, H., Saitoh, S., Nagata, K.I., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15368_A7I5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 ()
Sequence file: RDB15368_A7I5a.seq check
>D04868A1-1_A7I5_pCAG-F_V3_A01_01_ABI24.ab1
    1 CCTGGGGGCC AGGCTCCTTC GGGGGGGAGG GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG
   61 ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC
  121 CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT TAATACGACT
  181 CACTATAGGG AGACCCAAGC TAATCGATAT GGAGCAGAAG CTGATCTCCG AGGAGGACCT
  241 GATATCAAGC TTTGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCAGCCG AGCGCTCTAA
  301 AATGATCGAC AAGAACCTGC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TGAAGTTGCT
  361 TCTGTTAGGT GCTGGAGAAT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA
  421 TGAAGATGGC TACTCAGAAG AGGAGTGCCG GCAGTACCGT GCCGTGGTCT ACAGCAACAC
  481 CATCCAGTCT ATCATGGCCA TCGTGAAGGC CATGGGCAAC CTGCAGATCG ACTTTGCTGA
  541 CCCCCAGCGT GCGGATGATG CCAGGCAGCT GTTCGCCCTG TCCTGTGCTG CAGAGGAACA
  601 AGGGATGCTT CCTGAAGACC TGTCCGGTGT CATCCGGAGG CTCTGGGCTG ACCACGGTGT
  661 GCAAGCCTGC TTTGGCCGCT CACGAGAATA CCAGCTCAAT GACTCAGCCG CTTACTACCT
  721 GAATGATCTG GAGCGCATTG CACAGAGTGA CTACATCCCT ACACAGCACG ATGTGCTGCG
  781 GACCCGTGTG AAGACCACGG GCATCGTGGA AACACACTTC ACCTTCAAGG ACTTACACTT
  841 CAAGATGTTT GATGTGGGTG GTCAGCGGTC TGAGCGCAAG AAGTGGATCC ACTGCTTTGA
  901 GGCGTCACGG GCCATCATCT TCTGTGTCGC CTTGAGCGCC TATGACTTGG TGCTGGCTGA
  961 GGATGAGGAG ATGACGCATG CATGAGAGCA TGAAGCTGTT TGACAGCATC TGCAACAACA
 1021 GTGTTCCACA GACACTCAAT CATTCCTCTC TCAACAGAAG ACTGGTTGGA GAAAAGAATC
1081 ACCCAGAGCT CCTGGACATC TGGATTCCTT GATTACACGG GGGCTCAACC AAGGTATA
//
Primer: pCA-bgter
Sequence file: RDB15368_A7I5b.seq check
>D04868A1-1_A715_ pCA-bgter_A03_03_ABI24.ab1
    1 AGCACTGAAG TCCATAATTT TTGGCAGAGG GAAAAGATCT CAGTGGTATT TGTGAGCCAG
   61 GGCATTGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG GAGTGAATTC
  121 ATACGATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT GCATGCTCGA GGTTTAAACG
  181 CGGCCGCGGT ACCGCTAGAT CAGAAGAGGC CACAGTCCTT CAGGTTGTTC TTGATGATGA
  241 CGTCAGTGAC GGCATCGAAC ACAAACTGCA CGTTCTTGGT GTCGGTGGCG CACGTGAAGT
  301 GCGTGTAGAT CTCCTTGGTG TCTTTGCGCT TATTTAGATC CTCAAACTTG CTCTGGATGT
  361 AGCTGGCTGC CTCGTCGTAC TTGTTGGCCC CCGTGTACTC AGGGAAACAG ATGGTCAGGG
  421 AGCTCTGTGT GATCTTTTCT TCAAACAGGT CCTTCTTGTT GAGGAAGAGG ATGATGGAGG
  481 TGTCTGTGAA CCACTTGTTG TTGCAGATGC TGTCAAACAG CTTCATGCTC TCATGCATGC
  541 GGTTCATCTC CTCATCCTCA GCCAGCACCA AGTCATAGGC GCTCAAGGCG ACACAGAAGA
  601 TGATGGCCGT GACGCCCTCA AAGCAGTGGA TCCACTTCTT GCGCTCAGAC CGCTGACCAC
  661 CCACATCAAA CATCTTGAAG TGTAAGTCCT TGAAGGTGAA GTGTGTTTCC ACGATGCCCG
  721 TGGTCTTCAC ACGGGTCCGC AGCACATCCT GCTGTGTAGG GATGTAGTCA CTCTGTGCAA
  781 TGCGCTCCAG ATCATTCAGG TAGTAAGCGG CTGAGTCATT GAGCTGGTAT TCTCGTGAGC
  841 GGCCAAAGCA GGCTTGCACA CCGTGGTCAG CCCAGAGCCT CCGGATGACA CCGGACAGGT
  901 CTTCAGGAAG CATCCCTTGT TCCTCTGCAG CACAGGACAG GGCGAACAGC TGCCTGGCAT
  961 CATCCGCACG CTGGGGGTCA GCAAAGTCGA TCTGCAGGTT GCCCATGGGC CTTCACGATG
 1021 GCCATGATAG ACTGGGATGG TTGTGCTGTA GACACGGCAC GGGTACTGCC GGCACCTCCT
 1081 CTTCTGAGTT AGCATCTTCA TGATGATCTC ATCTGGCTGG ACGAATGTTG CTCTTTCCCT
1141 TGATTCCTCC AAG
//
Primer: SV40pro_F ()
Sequence file: RDB15368_A7I5c.seq check
>D04868A1-1_A7I5_SV40pro_F_C01_07_ABI24.ab1
    1 CCCTGGTCCT GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
  961 CCCAACCTGC CATCACGAGA TTTCGATCAC GCGCTTCTAT GAAAGGTGGG CTTCGGATCG
 1021 TTTTCGGGAC GGCGCCTGAT GATTCTCAGC GCGGGAATCT CATGCTGGAA GTCTCGCACT
1081 GACTGTATGC AGCTATATGC TACGATTTAA GGCCAGTAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898. [link to RRC of NBRP]