Resource data sheet
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pCAG-Myc-mG alpha i2

Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i2, myc-tag.

Catalog number RDB15368
Resource name pCAG-Myc-mG alpha i2
Clone info. Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i2, tagged with Myc at N-terminal, CAG promoter.
The CMV promoter region of pcDNA3 (Clontech) was replaced with the promoter/enhancer region of pCAGGS (provided by Dr J.Miyazaki) to generate pcCAG. Expression was confirmed by the depositor with Western blotting.
Vector backbone pCAG-Myc (plasmid)
Selectable markers Amp^r (E. coli) Neo^r (mammalian cells).
Gene/insert name mouse Gnai2 cDNA
Depositor Nagata, Koh-ichi |
 
Other clones in our bank mouse Gnai2 (NCBI Gene 14678) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)
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RDB15368 pCAG-Myc-mG alpha i2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15368_A7I5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 RDB15368_A7I5a.seq
>D04868A1-1_A7I5_pCAG-F_V3_A01_01_ABI24.ab1
    1 CCTGGGGGCC AGGCTCCTTC GGGGGGGAGG GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG
   61 ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC
  121 CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT TAATACGACT
  181 CACTATAGGG AGACCCAAGC TAATCGATAT GGAGCAGAAG CTGATCTCCG AGGAGGACCT
  241 GATATCAAGC TTTGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCAGCCG AGCGCTCTAA
  301 AATGATCGAC AAGAACCTGC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TGAAGTTGCT
  361 TCTGTTAGGT GCTGGAGAAT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA
  421 TGAAGATGGC TACTCAGAAG AGGAGTGCCG GCAGTACCGT GCCGTGGTCT ACAGCAACAC
  481 CATCCAGTCT ATCATGGCCA TCGTGAAGGC CATGGGCAAC CTGCAGATCG ACTTTGCTGA
  541 CCCCCAGCGT GCGGATGATG CCAGGCAGCT GTTCGCCCTG TCCTGTGCTG CAGAGGAACA
  601 AGGGATGCTT CCTGAAGACC TGTCCGGTGT CATCCGGAGG CTCTGGGCTG ACCACGGTGT
  661 GCAAGCCTGC TTTGGCCGCT CACGAGAATA CCAGCTCAAT GACTCAGCCG CTTACTACCT
  721 GAATGATCTG GAGCGCATTG CACAGAGTGA CTACATCCCT ACACAGCACG ATGTGCTGCG
  781 GACCCGTGTG AAGACCACGG GCATCGTGGA AACACACTTC ACCTTCAAGG ACTTACACTT
  841 CAAGATGTTT GATGTGGGTG GTCAGCGGTC TGAGCGCAAG AAGTGGATCC ACTGCTTTGA
  901 GGCGTCACGG GCCATCATCT TCTGTGTCGC CTTGAGCGCC TATGACTTGG TGCTGGCTGA
  961 GGATGAGGAG ATGACGCATG CATGAGAGCA TGAAGCTGTT TGACAGCATC TGCAACAACA
 1021 GTGTTCCACA GACACTCAAT CATTCCTCTC TCAACAGAAG ACTGGTTGGA GAAAAGAATC
1081 ACCCAGAGCT CCTGGACATC TGGATTCCTT GATTACACGG GGGCTCAACC AAGGTATA
//
Primer: pCA-bgter RDB15368_A7I5b.seq
>D04868A1-1_A715_ pCA-bgter_A03_03_ABI24.ab1
    1 AGCACTGAAG TCCATAATTT TTGGCAGAGG GAAAAGATCT CAGTGGTATT TGTGAGCCAG
   61 GGCATTGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG GAGTGAATTC
  121 ATACGATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT GCATGCTCGA GGTTTAAACG
  181 CGGCCGCGGT ACCGCTAGAT CAGAAGAGGC CACAGTCCTT CAGGTTGTTC TTGATGATGA
  241 CGTCAGTGAC GGCATCGAAC ACAAACTGCA CGTTCTTGGT GTCGGTGGCG CACGTGAAGT
  301 GCGTGTAGAT CTCCTTGGTG TCTTTGCGCT TATTTAGATC CTCAAACTTG CTCTGGATGT
  361 AGCTGGCTGC CTCGTCGTAC TTGTTGGCCC CCGTGTACTC AGGGAAACAG ATGGTCAGGG
  421 AGCTCTGTGT GATCTTTTCT TCAAACAGGT CCTTCTTGTT GAGGAAGAGG ATGATGGAGG
  481 TGTCTGTGAA CCACTTGTTG TTGCAGATGC TGTCAAACAG CTTCATGCTC TCATGCATGC
  541 GGTTCATCTC CTCATCCTCA GCCAGCACCA AGTCATAGGC GCTCAAGGCG ACACAGAAGA
  601 TGATGGCCGT GACGCCCTCA AAGCAGTGGA TCCACTTCTT GCGCTCAGAC CGCTGACCAC
  661 CCACATCAAA CATCTTGAAG TGTAAGTCCT TGAAGGTGAA GTGTGTTTCC ACGATGCCCG
  721 TGGTCTTCAC ACGGGTCCGC AGCACATCCT GCTGTGTAGG GATGTAGTCA CTCTGTGCAA
  781 TGCGCTCCAG ATCATTCAGG TAGTAAGCGG CTGAGTCATT GAGCTGGTAT TCTCGTGAGC
  841 GGCCAAAGCA GGCTTGCACA CCGTGGTCAG CCCAGAGCCT CCGGATGACA CCGGACAGGT
  901 CTTCAGGAAG CATCCCTTGT TCCTCTGCAG CACAGGACAG GGCGAACAGC TGCCTGGCAT
  961 CATCCGCACG CTGGGGGTCA GCAAAGTCGA TCTGCAGGTT GCCCATGGGC CTTCACGATG
 1021 GCCATGATAG ACTGGGATGG TTGTGCTGTA GACACGGCAC GGGTACTGCC GGCACCTCCT
 1081 CTTCTGAGTT AGCATCTTCA TGATGATCTC ATCTGGCTGG ACGAATGTTG CTCTTTCCCT
1141 TGATTCCTCC AAG
//
Primer: SV40pro_F RDB15368_A7I5c.seq
>D04868A1-1_A7I5_SV40pro_F_C01_07_ABI24.ab1
    1 CCCTGGTCCT GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
  961 CCCAACCTGC CATCACGAGA TTTCGATCAC GCGCTTCTAT GAAAGGTGGG CTTCGGATCG
 1021 TTTTCGGGAC GGCGCCTGAT GATTCTCAGC GCGGGAATCT CATGCTGGAA GTCTCGCACT
1081 GACTGTATGC AGCTATATGC TACGATTTAA GGCCAGTAC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

2019.02.24

GNP_filter3_RDBDEP_html_190131.pl