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pGL4-JF1_886-1146anti (#RDB15334)

Luciferase reporter of the mouse Ccnb1ip1, nt.886 to nt.1146, antisense orientation.

Clone info. Luciferase reporter of the mouse cyclin B1 interacting protein 1 (ccnb1ip1) promoter/enhancer [nt.886 to nt.1146, antisense orientation] was inserted, see Fig. 2A of Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pGL4.23 [luc2/minP] (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Amp^r
Gene/insert name mouse Ccnb1ip1 genomic DNA
Depositor|Developer Yoshida, Wataru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15334 pGL4-JF1_886-1146anti DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-JF1_886-1146anti was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15334).

Reference section:

Yoshida, W., Tomikawa, J., Inaki, M., Kimura, H., Onodera, M., Hata, K., Nakabayashi, K., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15334_A7Hmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB15334_A7Hma.seq check
>D04779A8_A7Hm_2_pGL4-4174F_B01_04_ABI24.ab1
    1 AATGGGGTCA GAACATTTCT CTGGCCTAAC TGGCCAAATG TGCTGCCAGA CTGCAACTCA
   61 ACGCTCATTT TAAGATTGAA CTCCTACACA AAATTTCTTT CAGGATCATG ACACAAAACA
  121 AACAAACACA GATAATTGAA ACGGTTTGGG CATGTCCTTG TTCTATTCCA AACAGTTTCC
  181 ATCCTGAGTA ACCTCCCGTC CTTTTGCAAC CTCAACATAG CTAACTTATG CAAGGTAATA
  241 ATAGGTCCAA TTATAAACAG TCTGTAACTA TCCAACTTAG AAATTGCCTT CAGGGCGGCC
  301 TCGGCGGCCA AGCTTAGACA CTAGAGGGTA TATAATGGAA GCTCGACTTC CAGCTTGGCA
  361 ATCCGGTACT GTTGGTAAAG CCACCATGGA AGATGCCAAA AACATTAAGA AGGGCCCAGC
  421 GCCATTCTAC CCACTCGAAG ACGGGACCGC CGGCGAGCAG CTGCACAAAG CCATGAAGCG
  481 CTACGCCCTG GTGCCCGGCA CCATCGCCTT TACCGACGCA CATATCGAGG TGGACATTAC
  541 CTACGCCGAG TACTTCGAGA TGAGCGTTCG GCTGGCAGAA GCTATGAAGC GCTATGGGCT
  601 GAATACAAAC CATCGGATCG TGGTGTGCAG CGAGAATAGC TTGCAGTTCT TCATGCCCGT
  661 GTTGGGTGCC CTGTTCATCG GTGTGGCTGT GGCCCCAGCT AACGACATCT ACAACGAGCG
  721 CGAGCTGCTG AACAGCATGG GCATCAGCCA GCCCACCGTC GTATTCGTGA GCAAGAAAGG
  781 GCTGCAAAAG ATCCTCAACG TGCAAAAGAA GCTACCGATC ATACAAAAGA TCATCATCAT
  841 GGATAGCAAG ACCGACTACC AGGGCTTCCA AAGCATGTAC ACCTTCGTGA CTTCCCATTT
  901 GCCACCCGGC TTCAACGAGT ACGACTTCGT GCCCGAGAGC TTCGACCGGG ACAAAACCAT
  961 CGCCCTGATC ATGAACAGTA GTGGCAGTAC CGGATTGCCC AAGGGCGTAG CCCTACCGCA
 1021 ACCGGCACCG CTTGTGTCCG ATTCAGTTCA ATGCCCGCGA CCCCCATCTT TCGCAATAGA
1081 TTCATTCCCC CGACACCGCT ATCCCTCAGC CGGGTGGGTG TGC
//
Primer: pGL4-136R ()
Region: insert 3'
Sequence file: RDB15334_A7Hmb.seq check
>D04779A8_A7Hm_2_pGL4-136R_C01_07_ABI24.ab1
    1 GTTTTTTGTT TTTGGCATCT TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTG
   61 GAAGTCGAGC TTCCATTATA TACCCTCTAG TGTCTAAGCT TGGCCGCCGA GGCCGCCCTG
  121 AAGGCAATTT CTAAGTTGGA TAGTTACAGA CTGTTTATAA TTGGACCTAT TATTACCTTG
  181 CATAAGTTAG CTATGTTGAG GTTGCAAAAG GACGGGAGGT TACTCAGGAT GGAAACTGTT
  241 TGGAATAGAA CAAGGACATG CCCAAACCGT TTCAATTATC TGTGTTTGTT TGTTTTGTGT
  301 CATGATCCTG AAAGAAATTT TGTGTAGGAG TTCAATCTTA AAATGAGCGT TGAGTTGCAG
  361 TCTGGCAGCA CATTTGGCCA GTTAGGCCAG AGAAATGTTC TGGCACCTGC ACTTGCACTG
  421 GGGACAGCCT ATTTTGCTAG TTTGTTTTGT TTCGTTTTGT TTTGATGGAG AGCGTATGTT
  481 AGTACTATCG ATTCACACAA AAAACCAACA CACAGATGTA ATGAAAATAA AGATATTTTA
  541 TTGCGGCCTG TCCAATACCT CCCGTACCTT AATATTACTT ACTTATCCTT GAGAGACGTA
  601 CTAGTAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG ACGAGTATGA GCATCCAACA
  661 TTTTCGTGTC GCACTCATTC CCTTCTTTGC GGCATTTTGC TTGCCTGTTT TTGCACACCC
  721 CGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAACTG GGTGCAAGAG TGGGCTATAT
  781 CGAACTGGAT CTCAATAGCG GCAAGATCCT TGAGTCTTTC CGCCCCCGAA GAACGATTCC
  841 CGATGATGAG CACTTTCAAA GTACTGCTAT GTGGCGCGGT GTTGTCCCGT ATAGACGCCG
  901 GGCAAGAGCA GCTTGGTCGC CGTATACACT ACTCACAAAA CGACTTGGTT TGAGTACTCG
  961 CCCGGTCACG GGAAAAAGCA TCTTTACGGA TGGGCATGAC GGTAAGAGAA TTTGTGTAGT
 1021 GCCTGCCCAT TTACCCATGA GCGGACACAA CCGCGGTGAA CCTTTACGTT CCTGACACGA
1081 TTCCGAAGAC CGTAAAGGAA GCTTGACTGC CATTTTCCTT TCC
//
Primer: pAxCALNL_F1 ()
Region: Luciferase 3'
Sequence file: RDB15334_A7Hmc.seq check
>D04779A8_A7Hm_2_pAxCALNLF1_D01_10_ABI24.ab1
    1 CAACAAAGGA TATTCTTATC AATGTCTGCT CGAAGCGGCC GGCCGCCCCG ACTCTAGAAT
   61 TATTACACGG CGATCTTGCC GCCCTTCTTG GCCTTAATGA GAATCTCGCG GATCTTGCGG
  121 GCGTCCAACT TGCCGGTCAG TCCTTTAGGC ACCTCGTCCA CGAACACAAC ACCACCGCGC
  181 AGCTTCTTGG CGGTTGTAAC CTGGCTGGCC ACATAGTCCA CGATCTCCTT CTCGGTCATG
  241 GTTTTACCGT GTTCCAGCAC GACGACTGCG GCGGGCAGCT CGCCGGCATC GTCGTCGGGC
  301 AGGCCGGCGA CCCCGGCGTC GAAGATGTTG GGGTGTTGCA GCAGGATGCT CTCCAGTTCG
  361 GCTGGGGCTA CCTGGTAGCC CTTGTATTTG ATCAGGCTCT TCAGCCGGTC CACGATGAAG
  421 AAGTGCTCGT CCTCGTCCCA GTAGGCGATG TCGCCGCTGT GCAGCCAGCC GTCCTTGTCG
  481 ATGAGAGCGT TTGTAGCCTC GGGGTTGTTA ACGTAGCCGC TCATGATCAT GGGGCCACGG
  541 ACGCACAGCT CGCCGCGCTG GTTCACACCC AGTGTCTTAC CGGTGTCCAA GTCCACCACC
  601 TTAGCCTCGA AGAAGGGCAC CACCTTGCCT ACTGCGCCAG GCTTGTCGTC CCCTTCGGGG
  661 GTGATCAGAA TGGCGCTGGT TGTTTCTGTC AGGCCGTAGC CCTGGCGGAT GCCTGGTAGG
  721 TGGAAGCGTT TGGCCACGGC CTCACCTACC TCCTTGCTGA GCGGCGCCCC GCCGCTGGCG
  781 ATCTCGTGCA AGTTGCTTAG GTCGTACTTG TCGATGAGAG TGCTCTTAGC GAAGAAGCTA
  841 AATAGTGTGG GCACCCAGCA GGGCAGATTG AATCTTTATA GTCTTGGCAA GCCTGCGCAA
  901 GAATAGCTCC TTCCTCGAAA GCGGTACAAT GAAGCACGAA CCCCGAAGCC CGCCAGATCC
  961 AAGTAAGCCC CAAGCCGGTT GGTGAAACAA TGCCCGGAAG CCGTTGATGA AATGGGCCAC
 1021 CCACGCCTGA AGGATAAGCC GGTGGTCGGG GGAATGGATT CCTGGGTTTG CACGAAAGAA
 1081 ATGGGGGTTC GCCCGGGCAA TGAACCTTGA AATTCCGGGA CACACCAAGA CCGGGGTGGC
1141 GACGTATTGC CGCGGGGTAA G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshida, W., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]