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pGL4-JF1_886-1146 (#RDB15333)

Luciferase reporter of the mouse Ccnb1ip1, nt.886 to nt.1146, sense orientation.

Clone info. Luciferase reporter of the mouse cyclin B1 interacting protein 1 (ccnb1ip1) promoter/enhancer [nt.886 to nt.1146, sense orientation] was inserted, see Fig. 2A of Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pGL4.23 [luc2/minP] (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Amp^r
Gene/insert name mouse Ccnb1ip1 genomic DNA
Depositor|Developer Yoshida, Wataru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15333 pGL4-JF1_886-1146 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-JF1_886-1146 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15333).

Reference section:

Yoshida, W., Tomikawa, J., Inaki, M., Kimura, H., Onodera, M., Hata, K., Nakabayashi, K., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15333_A7Hmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB15333_A7Hma.seq check
>D04779A7_A7Hm_1_pGL4-4174F_G12_21_ABI24.ab1
    1 ACAGGGGTCA GACATTTCTC TGGCCTAACT GGCCGCCCTG AAGGCAATTT CTAAGTTGGA
   61 TAGTTACAGA CTGTTTATAA TTGGACCTAT TATTACCTTG CATAAGTTAG CTATGTTGAG
  121 GTTGCAAAAG GACGGGAGGT TACTCAGGAT GGAAACTGTT TGGAATAGAA CAAGGACATG
  181 CCCAAACCGT TTCAATTATC TGTGTTTGTT TGTTTTGTGT CATGATCCTG AAAGAAATTT
  241 TGTGTAGGAG TTCAATCTTA AAATGAGCGT TGAGTTGCAG TCTGGCAGCA CATTTGGCCT
  301 CGGCGGCCAA GCTTAGACAC TAGAGGGTAT ATAATGGAAG CTCGACTTCC AGCTTGGCAA
  361 TCCGGTACTG TTGGTAAAGC CACCATGGAA GATGCCAAAA ACATTAAGAA GGGCCCAGCG
  421 CCATTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC CATGAAGCGC
  481 TACGCCCTGG TGCCCGGCAC CATCGCCTTT ACCGACGCAC ATATCGAGGT GGACATTACC
  541 TACGCCGAGT ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAAGCG CTATGGGCTG
  601 AATACAAACC ATCGGATCGT GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG
  661 TTGGGTGCCC TGTTCATCGG TGTGGCTGTG GCCCCAGCTA ACGACATCTA CAACGAGCGC
  721 GAGCTGCTGA ACAGCATGGG CATCAGCCAG CCCACCGTCG TATTCGTGAG CAAGAAAGGG
  781 CTGCAAAAGA TCCTCAACGT GCAAAGAAGC TACCGATCAT ACAAAAGATC ATCATCATGG
  841 ATAGCAAGAC CGACTACCAG GGCTTCCAAA GCATGTACAC CTTCGTGACT TCCCATTTGC
  901 CACCCGGCTT CAACGAGTAC GACTTCGTGC CCGAGAGCTT CGACCGGGAC AAAACCATCG
  961 CCCTGATCAT GAACAGTAGT GGCAGTACCG GGATTGCCCA AAGGGCGTAG CCCTACCCGC
 1021 ACCCGCACCG CTTGTGTCCG ATTTCAGTCA TGCCCGCGGA CCCCCATCCT TCCGGCCAAT
1081 AGATCAATTG TCGGACACCG GCTATCCATC AGCGCGATGG ATGCCCATTT TCAC
//
Primer: pGL4-136R ()
Region: insert 3'
Sequence file: RDB15333_A7Hmb.seq check
>D04779A7_A7Hm_1_pGL4-136R_H12_24_ABI24.ab1
    1 GTCTTTGTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTGGA
   61 AGTCGAGCTT CCATTATATA CCCTCTAGTG TCTAAGCTTG GCCGCCGAGG CCAAATGTGC
  121 TGCCAGACTG CAACTCAACG CTCATTTTAA GATTGAACTC CTACACAAAA TTTCTTTCAG
  181 GATCATGACA CAAAACAAAC AAACACAGAT AATTGAAACG GTTTGGGCAT GTCCTTGTTC
  241 TATTCCAAAC AGTTTCCATC CTGAGTAACC TCCCGTCCTT TTGCAACCTC AACATAGCTA
  301 ACTTATGCAA GGTAATAATA GGTCCAATTA TAAACAGTCT GTAACTATCC AACTTAGAAA
  361 TTGCCTTCAG GGCGGCCAGT TAGGCCAGAG AAATGTTCTG GCACCTGCAC TTGCACTGGG
  421 GACAGCCTAT TTTGCTAGTT TGTTTTGTTT CGTTTTGTTT TGATGGAGAG CGTATGTTAG
  481 TACTATCGAT TCACACAAAA AACCAACACA CAGATGTAAT GAAAATAAAG ATATTTTATT
  541 GCGGCCTGTC CAATACCTCC CGTACCTTAA TATTACTTAC TTATCCTTGA GAGACGTACT
  601 AGTAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAC GAGTATGAGC ATCCAACATT
  661 TTCGTGTCGC ACTCATTCCC TTCTTTGCGG CATTTTGCTT GCCTGTTTTT GCACACCCCG
  721 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAACTGGG TGCAAGAGTG GGCTATATCG
  781 AACTGGATCT CAATAGCGGC AAGATCCTTG AGTCTTTCCG CCCCGAAGAA CGATTCCCGA
  841 TGATGAGCAC TTTCAAAGTA CTGCTATGTG GCGCGGTGTT GTCCCGTATA GACGCCGGGC
  901 AAGAGCAGCT TGGTCGCCGT ATACACTACT CACAAAACGA CTTGGTTGAG TACTCGCCGG
  961 TCACGGAAAA GCATCTTACG GATGGCATGA CGGGTAAGAG AATTGTGTAG TGCTGCCATT
 1021 TACCATGAGC GACCACCACG CGGTCAACTT ACTTCTGACA CCGAATCGAG CCTATGAGCT
1081 GAACTGCATT CTTCATATAT GGGTGAATCA ATGTGACCCG GACTTGGAAT CG
//
Primer: pAxCALNL_F1 ()
Region: Luciferase 3'
Sequence file: RDB15333_A7Hmc.seq check
>D04779A7_A7Hm_1_pAxCALNLF1_A01_01_ABI24.ab1
    1 CTCATTTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC
   61 ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC
  121 AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC
  181 TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA
  241 CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG
  301 GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG
  361 GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC
  421 TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA
  481 GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC
  541 AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTAGCC
  601 TCGAAGAAGG GCACCACCTT GCCTACTGCG CCAGGCTTGT CGTCCCCTTC GGGGGTGATC
  661 AGAATGGCGC TGGTTGTTTC TGTCAGGCCG TAGCCCTGGC GGATGCCTGG TAGGTGGAAG
  721 CGTTTGGCCA CGGCCTCACC TACCTCCTTG CTGAGCGGCG CCCCGCCGCT GGCGATCTCG
  781 TGCAAGTTGC TTAGGTCGTA CTTGTCGATG AGAGTGCTCT TAGCGAAGAA GCTAAATAGT
  841 GTGGGCACCA GCAGGGCAGA TTGAATCTTA TAGTCTTGCA AGCTGCGCAA GAATAGCTCC
  901 TCCTCGAAGC GGTACATGAG CACGACCCGA AAGCCGCAGA TCAAGTAGCC CAGCGTGGTG
  961 AACATGCCGA AAGCCGTGGT GAAATGGCAC CACGCTGAGG ATAGCGGTGT TCGGGGATGG
 1021 ATCTGGCTGC CGAGAATGGG TTCGCGGGCC ATGGACTGAA TCGCACACAA AGTCGGTTGC
1081 CGGTTGCGGT AAGGGCCTAA CGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshida, W., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]