Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGL4-JF1_886-1146

Luciferase reporter of the mouse Ccnb1ip1, nt.886 to nt.1146, sense orientation.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15333_A7Hmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Region: insert 5' Sequence file: RDB15333_A7Hma.seq
>D04779A7_A7Hm_1_pGL4-4174F_G12_21_ABI24.ab1     1 ACAGGGGTCA GACATTTCTC TGGCCTAACT GGCCGCCCTG AAGGCAATTT CTAAGTTGGA    61 TAGTTACAGA CTGTTTATAA TTGGACCTAT TATTACCTTG CATAAGTTAG CTATGTTGAG   121 GTTGCAAAAG GACGGGAGGT TACTCAGGAT GGAAACTGTT TGGAATAGAA CAAGGACATG   181 CCCAAACCGT TTCAATTATC TGTGTTTGTT TGTTTTGTGT CATGATCCTG AAAGAAATTT   241 TGTGTAGGAG TTCAATCTTA AAATGAGCGT TGAGTTGCAG TCTGGCAGCA CATTTGGCCT   301 CGGCGGCCAA GCTTAGACAC TAGAGGGTAT ATAATGGAAG CTCGACTTCC AGCTTGGCAA   361 TCCGGTACTG TTGGTAAAGC CACCATGGAA GATGCCAAAA ACATTAAGAA GGGCCCAGCG   421 CCATTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC CATGAAGCGC   481 TACGCCCTGG TGCCCGGCAC CATCGCCTTT ACCGACGCAC ATATCGAGGT GGACATTACC   541 TACGCCGAGT ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAAGCG CTATGGGCTG   601 AATACAAACC ATCGGATCGT GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG   661 TTGGGTGCCC TGTTCATCGG TGTGGCTGTG GCCCCAGCTA ACGACATCTA CAACGAGCGC   721 GAGCTGCTGA ACAGCATGGG CATCAGCCAG CCCACCGTCG TATTCGTGAG CAAGAAAGGG   781 CTGCAAAAGA TCCTCAACGT GCAAAGAAGC TACCGATCAT ACAAAAGATC ATCATCATGG   841 ATAGCAAGAC CGACTACCAG GGCTTCCAAA GCATGTACAC CTTCGTGACT TCCCATTTGC   901 CACCCGGCTT CAACGAGTAC GACTTCGTGC CCGAGAGCTT CGACCGGGAC AAAACCATCG   961 CCCTGATCAT GAACAGTAGT GGCAGTACCG GGATTGCCCA AAGGGCGTAG CCCTACCCGC  1021 ACCCGCACCG CTTGTGTCCG ATTTCAGTCA TGCCCGCGGA CCCCCATCCT TCCGGCCAAT 1081 AGATCAATTG TCGGACACCG GCTATCCATC AGCGCGATGG ATGCCCATTT TCAC //
Primer: pGL4-136R Region: insert 3' Sequence file: RDB15333_A7Hmb.seq
>D04779A7_A7Hm_1_pGL4-136R_H12_24_ABI24.ab1     1 GTCTTTGTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTGGA    61 AGTCGAGCTT CCATTATATA CCCTCTAGTG TCTAAGCTTG GCCGCCGAGG CCAAATGTGC   121 TGCCAGACTG CAACTCAACG CTCATTTTAA GATTGAACTC CTACACAAAA TTTCTTTCAG   181 GATCATGACA CAAAACAAAC AAACACAGAT AATTGAAACG GTTTGGGCAT GTCCTTGTTC   241 TATTCCAAAC AGTTTCCATC CTGAGTAACC TCCCGTCCTT TTGCAACCTC AACATAGCTA   301 ACTTATGCAA GGTAATAATA GGTCCAATTA TAAACAGTCT GTAACTATCC AACTTAGAAA   361 TTGCCTTCAG GGCGGCCAGT TAGGCCAGAG AAATGTTCTG GCACCTGCAC TTGCACTGGG   421 GACAGCCTAT TTTGCTAGTT TGTTTTGTTT CGTTTTGTTT TGATGGAGAG CGTATGTTAG   481 TACTATCGAT TCACACAAAA AACCAACACA CAGATGTAAT GAAAATAAAG ATATTTTATT   541 GCGGCCTGTC CAATACCTCC CGTACCTTAA TATTACTTAC TTATCCTTGA GAGACGTACT   601 AGTAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAC GAGTATGAGC ATCCAACATT   661 TTCGTGTCGC ACTCATTCCC TTCTTTGCGG CATTTTGCTT GCCTGTTTTT GCACACCCCG   721 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAACTGGG TGCAAGAGTG GGCTATATCG   781 AACTGGATCT CAATAGCGGC AAGATCCTTG AGTCTTTCCG CCCCGAAGAA CGATTCCCGA   841 TGATGAGCAC TTTCAAAGTA CTGCTATGTG GCGCGGTGTT GTCCCGTATA GACGCCGGGC   901 AAGAGCAGCT TGGTCGCCGT ATACACTACT CACAAAACGA CTTGGTTGAG TACTCGCCGG   961 TCACGGAAAA GCATCTTACG GATGGCATGA CGGGTAAGAG AATTGTGTAG TGCTGCCATT  1021 TACCATGAGC GACCACCACG CGGTCAACTT ACTTCTGACA CCGAATCGAG CCTATGAGCT 1081 GAACTGCATT CTTCATATAT GGGTGAATCA ATGTGACCCG GACTTGGAAT CG //
Primer: pAxCALNL_F1 Region: Luciferase 3' Sequence file: RDB15333_A7Hmc.seq
>D04779A7_A7Hm_1_pAxCALNLF1_A01_01_ABI24.ab1     1 CTCATTTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC    61 ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC   121 AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC   181 TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA   241 CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG   301 GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG   361 GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC   421 TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA   481 GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC   541 AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTAGCC   601 TCGAAGAAGG GCACCACCTT GCCTACTGCG CCAGGCTTGT CGTCCCCTTC GGGGGTGATC   661 AGAATGGCGC TGGTTGTTTC TGTCAGGCCG TAGCCCTGGC GGATGCCTGG TAGGTGGAAG   721 CGTTTGGCCA CGGCCTCACC TACCTCCTTG CTGAGCGGCG CCCCGCCGCT GGCGATCTCG   781 TGCAAGTTGC TTAGGTCGTA CTTGTCGATG AGAGTGCTCT TAGCGAAGAA GCTAAATAGT   841 GTGGGCACCA GCAGGGCAGA TTGAATCTTA TAGTCTTGCA AGCTGCGCAA GAATAGCTCC   901 TCCTCGAAGC GGTACATGAG CACGACCCGA AAGCCGCAGA TCAAGTAGCC CAGCGTGGTG   961 AACATGCCGA AAGCCGTGGT GAAATGGCAC CACGCTGAGG ATAGCGGTGT TCGGGGATGG  1021 ATCTGGCTGC CGAGAATGGG TTCGCGGGCC ATGGACTGAA TCGCACACAA AGTCGGTTGC 1081 CGGTTGCGGT AAGGGCCTAA CGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl