Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-JF1_1128-1312anti (#RDB15332)

Luciferase reporter of the mouse Ccnb1ip1, nt.1128 to nt.1312, antisense orientation.

Clone info. Luciferase reporter of the mouse cyclin B1 interacting protein 1 (ccnb1ip1) promoter/enhancer [nt.1128 to nt.1312, antisense orientation] was inserted, see Fig. 2A of Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pGL4.23 [luc2/minP] (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Amp^r
Gene/insert name mouse Ccnb1ip1 genomic DNA
Depositor|Developer Yoshida, Wataru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15332 pGL4-JF1_1128-1312anti DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-JF1_1128-1312anti was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15332).

Reference section:

Yoshida, W., Tomikawa, J., Inaki, M., Kimura, H., Onodera, M., Hata, K., Nakabayashi, K., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15332_A7Hmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB15332_A7Hma.seq check
>D04779A6_A7Hm_2_pGL4-4174F_F04_16_ABI24.ab1
    1 GCTGGTCAGA CATTTCTCTG GCTACTGGCC CCTGATTGGC TGAGTTCAAG ATCTCCTCCT
   61 CCTCCTCCTC CTCCTCCTCC TCCTCCTCCT CCTCCTCTTC TTCCTCTTCC TCCTCCTCTT
  121 CTTCCTCTTC CTCCTCCTCT TCTTCTTTTT CTTCCTCCTT CTTCTCCCCC TCCTCCCTGT
  181 TCCCCTCTTA ATTTTAAAAT GTGCTGCCAG ACTGCGGCCT CGGCGGCCAA GCTTAGACAC
  241 TAGAGGGTAT ATAATGGAAG CTCGACTTCC AGCTTGGCAA TCCGGTACTG TTGGTAAAGC
  301 CACCATGGAA GATGCCAAAA ACATTAAGAA GGGCCCAGCG CCATTCTACC CACTCGAAGA
  361 CGGGACCGCC GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCAC
  421 CATCGCCTTT ACCGACGCAC ATATCGAGGT GGACATTACC TACGCCGAGT ACTTCGAGAT
  481 GAGCGTTCGG CTGGCAGAAG CTATGAAGCG CTATGGGCTG AATACAAACC ATCGGATCGT
  541 GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG
  601 TGTGGCTGTG GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG
  661 CATCAGCCAG CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCAAAAGA TCCTCAACGT
  721 GCAAAAGAAG CTACCGATCA TACAAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA
  781 GGGCTTCCAA AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCGGCT TCAACGAGTA
  841 CGACTTCGTG CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA TGAACAGTAG
  901 TGGCAGTACC GGGATTGCCC AAGGGCGTAG CCCTACCGCA CCGCACCCGC TTGTGTCCGA
  961 TTCAGTCATG CCCGCGACCC CCATCTTCGG GCAACCAGAT CATCCCCCGA CACCGGCTAT
 1021 CCTCAGCGTG GTGCCCAATT TTCACCCACG CCTCGGCATT GATTCAACCC ACGCTGGACT
1081 AACTGGATCC TGCGGGCCTT TCGGGATCGG TGGCCCTCCA AT
//
Primer: pGL4-136R ()
Region: insert 3'
Sequence file: RDB15332_A7Hmb.seq check
>D04779A6_A7Hm_2_pGL4-136R_F05_17_ABI24.ab1
    1 GACTTTGCAT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTGG
   61 AAGTCGAGCT TCCATTATAT ACCCTCTAGT GTCTAAGCTT GGCCGCCGAG GCCGCAGTCT
  121 GGCAGCACAT TTTAAAATTA AGAGGGGAAC AGGGAGGAGG GGGAGAAGAA GGAGGAAGAA
  181 AAAGAAGAAG AGGAGGAGGA AGAGGAAGAA GAGGAGGAGG AAGAGGAAGA AGAGGAGGAG
  241 GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGAGATCT TGAACTCAGC CAATCAGGGG
  301 CCAGTTAGGC CAGAGAAATG TTCTGGCACC TGCACTTGCA CTGGGGACAG CCTATTTTGC
  361 TAGTTTGTTT TGTTTCGTTT TGTTTTGATG GAGAGCGTAT GTTAGTACTA TCGATTCACA
  421 CAAAAAACCA ACACACAGAT GTAATGAAAA TAAAGATATT TTATTGCGGC CTGTCCAATA
  481 CCTCCCGTAC CTTAATATTA CTTACTTATC CTTGAGAGAC GTACTAGTAA CCCTGATAAA
  541 TGCTTCAATA ATATTGAAAA AGGACGAGTA TGAGCATCCA ACATTTTCGT GTCGCACTCA
  601 TTCCCTTCTT TGCGGCATTT TGCTTGCCTG TTTTTGCACA CCCCGAAACG CTGGTGAAAG
  661 TAAAAGATGC TGAAGATCAA CTGGGTGCAA GAGTGGGCTA TATCGAACTG GATCTCAATA
  721 GCGGCAAGAT CCTTGAGTCT TTCCGCCCCG AAGAACGATT CCCGATGATG AGCACTTTCA
  781 AAGTACTGCT ATGTGGCGCG GTGTTGTCCC GTATAGACGC CGGGCAAGAG CAGCTTGGTC
  841 GCCGTATACA CTACTCACAA AACGACTTGG TTGAGTACTC GCCGGTCACG GAAAAGCATC
  901 TTACGGATGG CATGACGGTA AGAGAATTGT GTAGTGCTGC CATTACCATG AGCGACAACA
  961 CCGCGGCCAA CTTACTTCTG ACAACGATCG GAGGCCCTAA GGAGCTGACT GCATTTCTTT
 1021 CATAATTATG GGTTGATCAT GTGACCCGGG CTTGACCGCT GGCAAACCCA GAGGTGACCG
1081 AAGTCATTAC CGGAAACCGA ACGAGCCGGT GAATATACCC AACGCGA
//
Primer: pAxCALNL_F1 ()
Region: Luciferase 3'
Sequence file: RDB15332_A7Hmc.seq check
>D04779A6_A7Hm_2_pAxCALNLF1_F12_18_ABI24.ab1
    1 AATAATGTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA
   61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC
  121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT
  181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT
  241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC
  301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG
  361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG
  421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG
  481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA
  541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC
  601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT
  661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA
  721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC
  781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG
  841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC
  901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGT
  961 GAACATGCCG AAGCCCGTGG TGAAATGGGC ACCACGCTGA GGATAGCGGT GTCGGGGATG
 1021 ATCTGGTTTG CGAGATGGGG GTCGCGGGGC ATGACTGAAT CCGGACACAG CGATGCGCTG
 1081 CGTAAGGCTA CGCCTTGTCA ATCCAGTACT GCCATACTGT TACATGAATC CAAGGGATCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshida, W., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]