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pGL4-JF1_886-1349anti (#RDB15330)

Luciferase reporter of the mouse Ccnb1ip1, nt.886 to nt.1349, antisense orientation.

Clone info. Luciferase reporter of the mouse cyclin B1 interacting protein 1 (ccnb1ip1) promoter/enhancer [nt.886 to nt.1349, antisense orientation] was inserted, see Fig. 2A of Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pGL4.23 [luc2/minP] (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Amp^r
Gene/insert name mouse Ccnb1ip1 genomic DNA
Depositor|Developer Yoshida, Wataru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15330 pGL4-JF1_886-1349anti DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-JF1_886-1349anti was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15330).

Reference section:

Yoshida, W., Tomikawa, J., Inaki, M., Kimura, H., Onodera, M., Hata, K., Nakabayashi, K., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15330_A7Hmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB15330_A7Hma.seq check
>D04779A4_A7Hm_1_pGL4-4174F_D04_10_ABI24.ab1
    1 GCGGGGTCAG ACATTTCTCT GGCTACTGGC CCAAGGACGG GAAGCTACCT AAGTAATGGG
   61 CCTCTGAACC TGATTGGCTG AGTTCAAGAT CTCCTCCTCC TCCTCCTCCT CCTCCTCCTC
  121 CTCCTCCTCC TCCTCTTCTT CCTCTTCCTC CTCCTCTTCT TCCTCTTCCT CCTCCTCTTC
  181 TTCTTTTTCT TCCTCCTTCT TCTCCCCCTC CTCCCTGTTC CCCTCTTAAT TTTAAAATGT
  241 GCTGCCAGAC TGCAACTCAA CGCTCATTTT AAGATTGAAC TCCTACACAA AATTTCTTTC
  301 AGGATCATGA CACAAAACAA ACAAACACAG ATAATTGAAA CGGTTTGGGC ATGTCCTTGT
  361 TCTATTCCAA ACAGTTTCCA TCCTGAGTAA CCTCCCGTCC TTTTGCAACC TCAACATAGC
  421 TAACTTATGC AAGGTAATAA TAGGTCCAAT TATAAACAGT CTGTAACTAT CCAACTTAGA
  481 AATTGCCTTC AGGGCGGCCT CGGCGGCCAA GCTTAGACAC TAGAGGGTAT ATAATGGAAG
  541 CTCGACTTCC AGCTTGGCAA TCCGGTACTG TTGGTAAAGC CACCATGGAA GATGCCAAAA
  601 ACATTAAGAA GGGCCCAGCG CCATTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC
  661 TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCAC CATCGCCTTT ACCGACGCAC
  721 ATATCGAGGT GGACATTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG
  781 CTATGAAGCG CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC GAGAATAGCT
  841 TGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG GCCCCAGCTA
  901 ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG CCCACCGGTC
  961 GTATTCGTGG AGCAAGAAAG GGCTGCAAAA AGATCCTCAC GTGCAAAAGA AGCTACCGAT
 1021 CATACAAAGA TCATCATCAT GGGATAGCAA GGAACGGACT AACCAAGGGC TTCCGAAGGC
1081 ATGGTACACC TTCGATGGAC CTTCCCGATT TGGCCCACCC CCGTGC
//
Primer: pGL4-136R ()
Region: insert 3'
Sequence file: RDB15330_A7Hmb.seq check
>D04779A4_A7Hm_1_pGL4-136R_D05_11_ABI24.ab1
    1 GCCTTATGCT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTGG
   61 AAGTCGAGCT TCCATTATAT ACCCTCTAGT GTCTAAGCTT GGCCGCCGAG GCCGCCCTGA
  121 AGGCAATTTC TAAGTTGGAT AGTTACAGAC TGTTTATAAT TGGACCTATT ATTACCTTGC
  181 ATAAGTTAGC TATGTTGAGG TTGCAAAAGG ACGGGAGGTT ACTCAGGATG GAAACTGTTT
  241 GGAATAGAAC AAGGACATGC CCAAACCGTT TCAATTATCT GTGTTTGTTT GTTTTGTGTC
  301 ATGATCCTGA AAGAAATTTT GTGTAGGAGT TCAATCTTAA AATGAGCGTT GAGTTGCAGT
  361 CTGGCAGCAC ATTTTAAAAT TAAGAGGGGA ACAGGGAGGA GGGGGAGAAG AAGGAGGAAG
  421 AAAAAGAAGA AGAGGAGGAG GAAGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGAGGAGG
  481 AGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGAT CTTGAACTCA GCCAATCAGG
  541 TTCAGAGGCC CATTACTTAG GTAGCTTCCC GTCCTTGGGC CAGTTAGGCC AGAGAAATGT
  601 TCTGGCACCT GCACTTGCAC TGGGGACAGC CTATTTTGCT AGTTTGTTTT GTTTCGTTTT
  661 GTTTTGATGG AGAGCGTATG TTAGTACTAT CGATTCACAC AAAAAACCAA CACACAGATG
  721 TAATGAAAAT AAAGATATTT TATTGCGGCC TGTCCAATAC CTCCCGTACC TTAATATTAC
  781 TTACTTATCC TTGAGAGACG TACTAGTAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
  841 GGACGAGTAT GAGCATCCAA CATTTTTCGT GTCGCACTCA TTCCCTTCTT TTGCGGCATT
  901 TTGCTTGCCC TGTTTTTGCA CACCCCGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC
  961 AACTGGGTTG CAAGAGTGGG CTATATCGAA CTGGATCTCA ATTAGCGGCA AGATCCCTTT
 1021 GAGTTCTTTC CGCCCCTCGA AGAAACCGAT TCCCGGATGG ATGGAAGCAA CTTTCCAAAG
1081 GTACCTGGCC TAATGTGGCC GCGATGTTTG ATTCCCCGGT ATAG
//
Primer: pAxCALNL_F1 ()
Region: Luciferase 3'
Sequence file: RDB15330_A7Hmc.seq check
>D04779A4_A7Hm_1_pAxCALNLF1_H10_22_ABI24.ab1
    1 TAAATGTATC TTATCATGTC TGCTCGAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA
   61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA
  121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT
  181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC
  241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG
  301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG
  361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT
  421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG
  481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA
  541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT
  601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA
  661 GAATGGCGCT GGTTGTTTCT GTCAGGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC
  721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT
  781 GCAAGTTGCT TAGGTCGTAC TTGTCGATGA GAGTGCTCTT AGCGAAGAAG CTAAATAGTG
  841 TGGGCACCAG CAGGGCAGAT TGAATCTTAT AGTCTTGCAA GCTGCGCAAG AATAGCTCCT
  901 CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC AGCGTGGTGA
  961 ACATGCCGAA AGCCGTGTGA AATGGCACCA CGCTGAGGAT AGCGTGTCGG GATGATCTGG
 1021 CTGCCGGAGA TGGGGTCGCG GCATGACTGA TTCGACACAA GCGTGCGGTT CGTAGGCTAC
1081 GCCCTGGTCA ATTCAGTTAC TGCCCAACTA AC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshida, W., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]