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pGL4-JF1_1-1799anti (#RDB15328)

Luciferase reporter of the mouse Ccnb1ip1, nt.1 to nt.1799, antisense orientation.

Clone info. Luciferase reporter of the mouse cyclin B1 interacting protein 1 (ccnb1ip1) promoter/enhancer [nt.1 to nt.1799, antisense orientation] was inserted, see Fig. 2A of Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pGL4.23 [luc2/minP] (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Amp^r
Gene/insert name mouse Ccnb1ip1 genomic DNA
Depositor|Developer Yoshida, Wataru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15328 pGL4-JF1_1-1799anti DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-JF1_1-1799anti was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15328).

Reference section:

Yoshida, W., Tomikawa, J., Inaki, M., Kimura, H., Onodera, M., Hata, K., Nakabayashi, K., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15328_A7Hmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB15328_A7Hma.seq check
>D04779A2_A7Hm_2_pGL4-4174F_A11_02_ABI24.ab1
    1 AATCAGGGTC AGACATTTCT CTGGCCTAAC TGGCCACCAA AGACAGAGGC TGCACTCACC
   61 CTCCGAGACC GCCCAGGTTA GCGCCGGGGT AGTAACGGTG CCTTTCCCAA TTAGAAAAAA
  121 AAAAAAAAAA AAAAAAAAAA AAAGAATACA GACGGATTTC CCCAGATTCA GGAACACTGT
  181 GCCCGAGGCA GAATGTATTG TTTCTGGGTC ACCTGGTCTG GGGAAAGCTG GCAAACAAGG
  241 ACTGCCGAGG CCCACGTGGA CCAAACCAGG TACTCCACCT GCCTGCCCTC CCCTAACCTC
  301 CCAAAGCAGA AACGTATCTC TTCTCAACTC ACAAACTCCA GCGCTGACAT GAAAAAAAAA
  361 AAGTCTTTCC CTCGGTTAGA CCCCTCAAGA AAATGAGAAA TTAAAAACCA GTGATCCGAG
  421 TTAAAAACCA GAGATCCAAA TTAACTGTGC ACTTACCAGA CCATCAGAAG CGAGAGATAG
  481 AGAAGGTTCA AGGACGGGAA GCTACCTAAG TAATGGGCCT CTGAACCTGA TTGGCTGAGT
541 TCAAGATCTC CTCCTCCTCC TCCTCCTCCT CCTCCACC
//
Primer: pGL4-136R ()
Region: insert 3'
Sequence file: RDB15328_A7Hmb.seq check
>D04779A2_A7Hm_2_pGL4-136R_B11_05_ABI24.ab1
    1 GGACTTTGTT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTGG
   61 AAGTCGAGCT TCCATTATAT ACCCTCTAGT GTCTAAGCTT GGCCGCCGAG GCCGGATCAA
  121 TCCACCTCCC TTTTCCTCCC CAGTGCCAGG ATTAAAAGTG TGTGCCACCT TTCCTGGTTC
  181 ATAATGCCTT TTCATTTATT TATTTTTAAT AGATGAGTGA TATTCCATTG TATAGTACCA
  241 CATTTTTTTT CCTGATCCAT TTATCTCTTG ATGGATGTCT AGGCTATTTC TAATTCCTGG
  301 CTATTATGTA CAGCAACAGT GACCATGGAT GAACAAGTGC CTTTGTAGCA GACAGACACA
  361 TCCTTTGGGT GTATGCCTAA GAGTGGTATA CAGCTGGAAT CTGAGGAGAC TCTGTTCCCA
  421 GCTTCCTGAG GAATGCACAG TGATTTCCAC AGTGGCTGTG CAAGCTTTCA CTTCCCCAGT
  481 AATAGACGCA TGTTTCCCTT TCCCTACAGC TTTGCCAGCA TTGTTCTATT TGTTCTATTG
  541 ATCTTGGTCC TCTAATGTGG GTAAGGTGGA GTAGTTTGGA TTGGCATTTC CCTGATAACT
  601 ACGGATGCTG ACCATTGCTT CGGTGATCCT GAATCATTTG CCTTTCATCC TTTGAGATTT
  661 CTTTGTTTAG ATATGGGATA TTCATCTTCT TGATGTGCAG TTTTTAAAGC TTTGAATGTA
  721 TTTTAGATAT TAATCCTCTA TTGGAATGTA CTTGGTAAAG ATCTTTTCCC ATTCTATAAA
  781 GTGCTTTTTT TTTTTGTCTG AATGATGGTG TTTGCTATAC AGATGCCACA TAAAGTTTAT
  841 ATCAAGGGCC AACCTGTTAA CCCAACCCTT GTTGTACTAC AATATTTTAA GTTGGGGGGA
  901 ATGAAGCCTT AGATGTGTAC AAGTATTCAA ATTTTATCTT GAAAGGATAA GCATTAAACC
  961 ACCCATTAAT AATTTGTATT TGTCTGTTTA GCTTTGAAGT TGCCCTGAAG GCAATTCTAA
 1021 GTTGGGATAG TTACAGACTG GTTTATAGTG GACCTATTAT TACCATGCAT AGTAAGCTAT
1081 GGTGAGTTGC AAAATGAGGT ACTCAGGATG CAACTGTTTT GGCAATTAGA
//
Primer: pAxCALNL_F1 ()
Region: Luciferase 3'
Sequence file: RDB15328_A7Hmc.seq check
>D04779A2_A7Hm_2_pAxCALNLF1_C11_08_ABI24.ab1
    1 ATAATATATC TTATCATGTC TGCTCGAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA
   61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA
  121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT
  181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC
  241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG
  301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG
  361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT
  421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG
  481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA
  541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT
  601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA
  661 GAATGGCGCT GGTTGTTTCT GTCAGGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC
  721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT
  781 GCAAGTTGCT TAGGTCGTAC TTGTCGATGA GAGTGCTCTT AGCGAAGAAG CTAAATAGTG
  841 TGGGCACCAG CAGGGCAGAT TGAATCTTAT AGTCTTGCAA GCTGCGCAAG AATAGCTCCT
  901 CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC AGCGTGATGA
  961 ACATGCCGAA GCCCGTGATG AAATGGCACC ACGCTGAGAT AGCGTGTCGG GGATGATCTG
 1021 GTTGCGAGAT GGGGTCGCGG GCATGACTGA TTCGACACAA GCGATGCGTT GCGTACGCTA
1081 CGTCCTGGCA TCAGTTACTG CCCACTACTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshida, W., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]