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pGL4-JF1_1-1799 (#RDB15327)

Luciferase reporter of the mouse Ccnb1ip1, nt.1 to nt.1799, sense orientation.

Clone info. Luciferase reporter of the mouse cyclin B1 interacting protein 1 (ccnb1ip1) promoter/enhancer [nt.1 to nt.1799, sense orientation] was inserted, see Fig. 2A of Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pGL4.23 [luc2/minP] (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Amp^r
Gene/insert name mouse Ccnb1ip1 genomic DNA
Depositor|Developer Yoshida, Wataru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15327 pGL4-JF1_1-1799 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-JF1_1-1799 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15327).

Reference section:

Yoshida, W., Tomikawa, J., Inaki, M., Kimura, H., Onodera, M., Hata, K., Nakabayashi, K., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15327_A7Hmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB15327_A7Hma.seq check
>D04779A1_A7Hm_2_pGL4-4174F_E10_13_ABI24.ab1
    1 AATAGGTCAG GACATTTCTC TGGCCTACTG GCCGGATCAA TCCACCTCCC TTTTCCTCCC
   61 CAGTGCCAGG ATTAAAAGTG TGTGCCACCT TTCCTGGTTC ATAATGCCTT TTCATTTATT
  121 TATTTTTAAT AGATGAGTGA TATTCCATTG TATAGTACCA CATTTTTTTT CCTGATCCAT
  181 TTATCTCTTG ATGGATGTCT AGGCTATTTC TAATTCCTGG CTATTATGTA CAGCAACAGT
  241 GACCATGGAT GAACAAGTGC CTTTGTAGCA GACAGACACA TCCTTTGGGT GTATGCCTAA
  301 GAGTGGTATA CAGCTGGAAT CTGAGGAGAC TCTGTTCCCA GCTTCCTGAG GAATGCACAG
  361 TGATTTCCAC AGTGGCTGTG CAAGCTTTCA CTTCCCCAGT AATAGACGCA TGTTTCCCTT
  421 TCCCTACAGC TTTGCCAGCA TTGTTCTATT TGTTCTATTG ATCTTGGTCC TCTAATGTGG
  481 GTAAGGTGGA GTAGTTTGGA TTGGCATTTC CCTGATAACT ACGGATGCTG ACCATTGCTT
  541 CGGTGATCCT GAATCATTTG CCTTTCATCC TTTGAGATTT CTTTGTTTAG ATATGGGATA
  601 TTCATCTTCT TGATGTGCAG TTTTTAAAGC TTTGAATGTA TTTTAGATAT TAATCCTCTA
  661 TTGGAATGTA CTTGGTAAAG ATCTTTTCCC ATTCTATAAA GTGCTTTTTT TTTTTGTCTG
  721 AATGATGGTG TTTGCTATAC AGATGCCACA TAAAGTTTAT ATCAAGGGCC AACCTGTTAA
  781 CCCAACCCTT GTTGTACTAC AATATTTTAA GTTGGGGGAA TGAAGCCTTA GATGTGTACA
  841 AGTATTCAAA TTTTATCTTG AAGGATAAGC ATTAAACCAC CCATTAATAA TTGTATTGTC
  901 TGTTAGCTTG AAGGTTGCCC TGAAAGGCAA TTTCTAAGTT GGATAGTTAC AGACTGTTTA
  961 TAATTGGACC TATTATTACC CTTGCATAAG TTAGCTATGT TGAGGTTGCA AAAGACGGAG
 1021 GTTACTCAGA TGGAAACTGT TTGGAATAGA ACAACGACAT GCCCAAACCG TTTCATTATC
1081 TGTGTTGATT TTGGTTGTGT CAATGCATCC TGCAAAAGAA AA
//
Primer: pGL4-136R ()
Region: insert 3'
Sequence file: RDB15327_A7Hmb.seq check
>D04779A1_A7Hm_2_pGL4-136R_F10_16_ABI24.ab1
    1 GGCTTTGATT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTGG
   61 AAGTCGAGCT TCCATTATAT ACCCTCTAGT GTCTAAGCTT GGCCGCCGAG GCCACCAAAG
  121 ACAGAGGCTG CACTCACCCT CCGAGACCGC CCAGGTTAGC GCCGGGGTAG TAACGGTGCC
  181 TTTCCCAATT AGAAAAAAAA AAAAAAAAAA AAAAGAATAC AGACGGATTT CCCCAGATTC
  241 AGGAACACTG TGCCCGAGGC AGAATGTATT GTTTCTGGGT CACCTGGTCT GGGGAAAGCT
  301 GGCAAACAAG GACTGCCGAG GCCCACGTGG ACCAAACCAG GTACTCCACC TGCCTGCCCT
  361 CCCCTAACCT CCCAAAGCAG AAACGTATCT CTTCTCAACT CACAAACTCC AGCGCTGACA
  421 TGAAAAAAAA AAGTCTTTCC CTCGGTTAGA CCCCTCAAGA AAATGAGAAA TTAAAAACCA
  481 GTGATCCGAG TTAAAAACCA GAGATCCAAA TTAACTGTGC ACTTACCAGA CCATCAGAAG
  541 CGAGAGATAG AGAAGGTTCA AGGACGGGAA GCTACCTAAG TAATGGGCCT CTGAACCTGA
601 TTGGCTGAGT TCAAGATCTC CTCCTCCTCC TCCTCCTCCT CCTCCTCCAC CT
//
Primer: pAxCALNL_F1 ()
Region: Luciferase 3'
Sequence file: RDB15327_A7Hmc.seq check
>D04779A1_A7Hm_2_pAxCALNLF1_G10_19_ABI24.ab1
    1 ACAATGCATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC
   61 ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC
  121 AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC
  181 TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA
  241 CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG
  301 GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG
  361 GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC
  421 TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA
  481 GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC
  541 AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTAGCC
  601 TCGAAGAAGG GCACCACCTT GCCTACTGCG CCAGGCTTGT CGTCCCCTTC GGGGGTGATC
  661 AGAATGGCGC TGGTTGTTTC TGTCAGGCCG TAGCCCTGGC GGATGCCTGG TAGGTGGAAG
  721 CGTTTGGCCA CGGCCTCACC TACCTCCTTG CTGAGCGGCG CCCCGCCGCT GGCGATCTCG
  781 TGCAAGTTGC TTAGGTCGTA CTTGTCGATG AGAGTGCTCT TAGCGAAGAA GCTAAATAGT
  841 GTGGGCACCA GCAGGGCAGA TTGAATCTTA TAGTCTTGCA AGCTGCGCAA GAATAGCTCC
  901 TCCTCGAAGC GGTACATGAG CACGACCCGA AAGCCGCAGA TCAAGTAGCC CAGCGTGGTG
  961 AACATGCCGA AAGCCGTGGT GAAATGGCAC CACGCTGAGA TAGCGGTGTC GGGGAATGAT
 1021 CTGGATTGCC GAGATGGGGG TCGCGGGCAT GACCTGGATT CGACACAGCG ATGCGGGTGC
1081 GGTACGCTAC GCCGTTGGGC AATTCCGGTT ACTTGACCCA CCTAACG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshida, W., An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species. PLoS One 10 (6): e0131204 (2015). PMID 26110280. [link to RRC of NBRP]