RDB15322 - CS-CMV-Bsd-IRES-Usp10

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CS-CMV-Bsd-IRES-Usp10_95-792

Lentiviral vector of USP10 [95-792 truncated mutant] after BSD-IRES. Gateway(R) expression vector.

Catalog number	RDB15322
Resource name	CS-CMV-Bsd-IRES-Usp10_95-792
Clone info.	Lentiviral vector of mouse ubiquitin-specific peptidase 10 (USP10) [95-792 truncated mutant] after blasticidin deaminase (Bsd)-IRES. Gateway(R) expression vector.
Comment	Expression was confirmed by the depositor with western blotting. CS-CMV-RfA is a lentiviral Gateway destination vector constructed by inserting Gateway reading frame cassette A into the NheI and HpaI sites of CSII-CMV-MCS-IRES2-Bsd.
Vector backbone	CS-CMV-RfA (plasmid)
Selectable markers	Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name	mouse Usp10 cDNA
Depositor\|Developer	Higuchi, Masaya \| Fujii, Masahiro \|

Other clones in our bank

mouse Usp10

External Database
mouse Usp10

Reference sequence

Lentiviral plasmid list. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested.(Stem Cell Reports 7(6), 1116-1129, 2016)

Additional terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.

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Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]

Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested.(Stem Cell Reports 7(6), 1116-1129, 2016)
Additional terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Stem Cell Reports 7(6), 1116-1129, 2016)。
付加的提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB15322	CS-CMV-Bsd-IRES-Usp10_95-792	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Original reference

original	Higuchi, M., USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells. Stem Cell Reports 7 (6): 1116-1129 (2016). PMID 27974222.

Further references such as user reports and related articles (go to bottom)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Higuchi, M., USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells. Stem Cell Reports 7 (6): 1116-1129 (2016). PMID 27974222.

2022.05.17

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