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CS-CMV-Bsd-IRES-Usp10_77-792

Lentiviral vector of USP10 [77-792 truncated mutant] after BSD-IRES. Gateway(R) expression vector.

Catalog number RDB15321
Resource name CS-CMV-Bsd-IRES-Usp10_77-792
Clone info. Lentiviral vector of mouse ubiquitin-specific peptidase 10 (USP10) [77-792 truncated mutant] after blasticidin deaminase (Bsd)-IRES. Gateway(R) expression vector.
Comment Expression was confirmed by the depositor with western blotting. CS-CMV-RfA is a lentiviral Gateway destination vector constructed by inserting Gateway reading frame cassette A into the NheI and HpaI sites of CSII-CMV-MCS-IRES2-Bsd.
Vector backbone CS-CMV-RfA (plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name mouse Usp10 cDNA
Depositor|Developer Higuchi, Masaya | Fujii, Masahiro |
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mouse Usp10

          Reference sequence
            
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          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested.(Stem Cell Reports 7(6), 1116-1129, 2016)
          Additional terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
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          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Remarks
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Stem Cell Reports 7(6), 1116-1129, 2016)。
          付加的提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考
          Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB15321 CS-CMV-Bsd-IRES-Usp10_77-792 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
          Ordering instruction of plasmids [in Japanese] [in English]

          References and tips

          Electronic file

          Original reference

          original Higuchi, M., USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells. Stem Cell Reports 7 (6): 1116-1129 (2016). PMID 27974222.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Higuchi, M., USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells. Stem Cell Reports 7 (6): 1116-1129 (2016). PMID 27974222.

          2022.05.17

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