Resource data sheet
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CS-CMV-Bsd-IRES-Usp10_WT

Lentiviral vector of USP10 [wild type] after BSD-IRES. Gateway(R) expression vector.

Catalog number RDB15320
Resource name CS-CMV-Bsd-IRES-Usp10_WT
Clone info. Lentiviral vector of mouse ubiquitin-specific peptidase 10 (USP10) [wild type] after blasticidin deaminase (Bsd)-IRES. Gateway(R) expression vector.
Expression was confirmed by the depositor with western blotting. CS-CMV-RfA is a lentiviral Gateway destination vector constructed by inserting Gateway reading frame cassette A into the NheI and HpaI sites of CSII-CMV-MCS-IRES2-Bsd.
Vector backbone CS-CMV-RfA (plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name mouse Usp10 cDNA
Originator Higuchi, Masaya | Fujii, Masahiro |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested.(Stem Cell Reports 7(6), 1116-1129, 2016)
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Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15320 CS-CMV-Bsd-IRES-Usp10_WT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Higuchi, M., USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells. Stem Cell Reports 7 (6): 1116-1129 (2016). PMID 27974222.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Higuchi, M., USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells. Stem Cell Reports 7 (6): 1116-1129 (2016). PMID 27974222.

2019.07.17

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