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YCpL-MID1-HAx4

Expression vector of S. cerevisiae MID1, tagged with 4xHA.

Catalog number RDB15300
Resource name YCpL-MID1-HAx4
Clone info. Expression vector of S. cerevisiae MID1, tagged with C-terminal 4xHA, single copy vector, under the MID1 promoter and ADH1 terminator with the LEU2 marker.
Expression was confirmed by the depositor with western blotting.
Vector backbone YCplac111 (plasmid)
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae).
Gene/insert name Saccharomyces cerevisiae MID1 genomic
Depositor Iida, Hidetoshi |
 
Other clones in our bank Saccharomyces cerevisiae MID1 (KEGG orthology K08284) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Cho, T. et al., Genes Cells 22(1), 94-104, 2017).
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RDB15300 YCpL-MID1-HAx4 Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Cho, T., Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+ -ATPase in yeast. Genes Cells 22 (1): 94-104 (2017). PMID 27935186.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Cho, T., Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+ -ATPase in yeast. Genes Cells 22 (1): 94-104 (2017). PMID 27935186.

2019.07.17

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