The NcoI/NotI EGFP fragment was inserted into the SalI/NotI sites to construct EGFP-tagged Cch1. Expression vector of S. cerevisiae CCH1, fused with EGFP.
Catalog number | RDB15298 |
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Resource name | pBCT-CCH1H-EGFP |
Clone info. | Expression vector of S. cerevisiae CCH1, fused with EGFP, under the TDH3 promoter and ADH1 terminator with the LEU2 marker. Expression was confirmed by the depositor with fluorescence confocal images. |
Vector backbone | pBC111 (plasmid) |
Selectable markers | Amp^r (E. coli), LEU2 (S. cerevisiae). |
Gene/insert name | Saccharomyces cerevisiae CCH1 genomic |
Depositor | Iida, Hidetoshi | |
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Other clones in our bank | Saccharomyces cerevisiae CCH1 (KEGG orthology K21864) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Cho, T. et al., Genes Cells 22(1), 94-104, 2017). |
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Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
Remarks | Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15298 | pBCT-CCH1H-EGFP | Under QC test. Please contact us. | DNA solution |
Electronic file
Original reference
original | Cho, T., Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+ -ATPase in yeast. Genes Cells 22 (1): 94-104 (2017). PMID 27935186. |
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Further references such as user reports and related articles (go to bottom)
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This clone will be sequenced a portion and digested by restriction enzyme for examination.
Original, user report and related articles
original | Cho, T., Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+ -ATPase in yeast. Genes Cells 22 (1): 94-104 (2017). PMID 27935186. |
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2019.07.17
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