Resource data sheet
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pBCT-CCH1H-EGFP (#RDB15298)

The NcoI/NotI EGFP fragment was inserted into the SalI/NotI sites to construct EGFP-tagged Cch1. Expression vector of S. cerevisiae CCH1, fused with EGFP.

Clone info. Expression vector of S. cerevisiae CCH1, fused with EGFP, under the TDH3 promoter and ADH1 terminator with the LEU2 marker.
Comment Expression was confirmed by the depositor with fluorescence confocal images.
Vector backbone pBC111 (plasmid)
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae).
Gene/insert name Saccharomyces cerevisiae CCH1 genomic
Depositor|Developer Iida, Hidetoshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Cho, T. et al., Genes Cells 22(1), 94-104, 2017).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cho, T. et al., Genes Cells 22(1), 94-104, 2017)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15298 pBCT-CCH1H-EGFP Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pBCT-CCH1H-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15298).

Reference section:

Cho, T., Ishii-Kato, A., Fukata, Y., Nakayama, Y., Iida, K., Fukata, M., Iida, H., Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+ -ATPase in yeast. Genes Cells 22 (1): 94-104 (2017). PMID 27935186. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Cho, T., Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+ -ATPase in yeast. Genes Cells 22 (1): 94-104 (2017). PMID 27935186. [link to RRC of NBRP]