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pBCT-CCH1H-EGFP

The NcoI/NotI EGFP fragment was inserted into the SalI/NotI sites to construct EGFP-tagged Cch1. Expression vector of S. cerevisiae CCH1, fused with EGFP.

Catalog number RDB15298
Resource name pBCT-CCH1H-EGFP
Clone info. Expression vector of S. cerevisiae CCH1, fused with EGFP, under the TDH3 promoter and ADH1 terminator with the LEU2 marker.
Comment Expression was confirmed by the depositor with fluorescence confocal images.
Vector backbone pBC111 (plasmid)
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae).
Gene/insert name Saccharomyces cerevisiae CCH1 genomic
Depositor|Developer Iida, Hidetoshi |
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External Database
Saccharomyces cerevisiae CCH1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Cho, T. et al., Genes Cells 22(1), 94-104, 2017).
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cho, T. et al., Genes Cells 22(1), 94-104, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB15298 pBCT-CCH1H-EGFP Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Cho, T., Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+ -ATPase in yeast. Genes Cells 22 (1): 94-104 (2017). PMID 27935186.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Cho, T., Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+ -ATPase in yeast. Genes Cells 22 (1): 94-104 (2017). PMID 27935186.

          2022.07.13

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