RDB15298 - pBCT-CCH1H-EGFP

Resource data sheet
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pBCT-CCH1H-EGFP

The NcoI/NotI EGFP fragment was inserted into the SalI/NotI sites to construct EGFP-tagged Cch1. Expression vector of S. cerevisiae CCH1, fused with EGFP.


Catalog number	RDB15298
Resource name	pBCT-CCH1H-EGFP
Clone info.	Expression vector of S. cerevisiae CCH1, fused with EGFP, under the TDH3 promoter and ADH1 terminator with the LEU2 marker. Expression was confirmed by the depositor with fluorescence confocal images.
Vector backbone	pBC111 (plasmid)
Selectable markers	Amp^r (E. coli), LEU2 (S. cerevisiae).
Gene/insert name	Saccharomyces cerevisiae CCH1 genomic
Depositor	Iida, Hidetoshi \|
Other clones in our bank	Saccharomyces cerevisiae CCH1 (KEGG orthology K21864) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Cho, T. et al., Genes Cells 22(1), 94-104, 2017).

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]

Remarks
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Cho, T. et al., Genes Cells 22(1), 94-104, 2017).
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cho, T. et al., Genes Cells 22(1), 94-104, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB15298	pBCT-CCH1H-EGFP	Under QC test. Please contact us.	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original	Cho, T., Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+ -ATPase in yeast. Genes Cells 22 (1): 94-104 (2017). PMID 27935186.

Further references such as user reports and related articles (go to bottom)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

Original, user report and related articles

original	Cho, T., Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+ -ATPase in yeast. Genes Cells 22 (1): 94-104 (2017). PMID 27935186.

2019.07.17

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