RDB15296 - pBC111

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pBC111

S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli.

Catalog number	RDB15296
Resource name	pBC111
Clone info.	S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli, delivative of the YCplac111. The ColE1 ori of YCplac111 have been replaced with the ori and rop derived from pBR322. Refer the Experimental Procedures of Iida, K. et al. J. Biol. Chem., 282 (35): 25659-25667, 2007. PMID: 17569661.
Vector backbone	pBC111 (plasmid)
Selectable markers	Amp^r (E. coli), LEU2 (S. cerevisiae).
Depositor\|Developer	Iida, Hidetoshi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Cho, T. et al., Genes Cells 22(1), 94-104, 2017).

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Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Cho, T. et al., Genes Cells 22(1), 94-104, 2017).
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cho, T. et al., Genes Cells 22(1), 94-104, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB15296	pBC111	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original	Iida, K., Essential, completely conserved glycine residue in the domain III S2-S3 linker of voltage-gated calcium channel alpha1 subunits in yeast and mammals. J. Biol. Chem. 282 (35): 25659-25667 (2007). PMID 17569661.

Further references such as user reports and related articles (go to bottom)

Featured content	Expression Vector Backbone of Saccharomyces cerevisiae (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Iida, K., Essential, completely conserved glycine residue in the domain III S2-S3 linker of voltage-gated calcium channel alpha1 subunits in yeast and mammals. J. Biol. Chem. 282 (35): 25659-25667 (2007). PMID 17569661.

2020.10.15

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