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pBC111

S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli.

Catalog number RDB15296
Resource name pBC111
Clone info. S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli, delivative of the YCplac111.
The ColE1 ori of YCplac111 have been replaced with the ori and rop derived from pBR322. Refer the Experimental Procedures of Iida, K. et al. J. Biol. Chem., 282 (35): 25659-25667, 2007. PMID: 17569661.
Vector backbone pBC111 (plasmid)
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae).
Depositor Iida, Hidetoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Cho, T. et al., Genes Cells 22(1), 94-104, 2017).
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RDB15296 pBC111 Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Iida, K., Essential, completely conserved glycine residue in the domain III S2-S3 linker of voltage-gated calcium channel alpha1 subunits in yeast and mammals. J. Biol. Chem. 282 (35): 25659-25667 (2007). PMID 17569661.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Iida, K., Essential, completely conserved glycine residue in the domain III S2-S3 linker of voltage-gated calcium channel alpha1 subunits in yeast and mammals. J. Biol. Chem. 282 (35): 25659-25667 (2007). PMID 17569661.

2019.07.17

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