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pBC111

S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli.

Catalog number RDB15296
Resource name pBC111
Clone info. S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli, delivative of the YCplac111.
Comment The ColE1 ori of YCplac111 have been replaced with the ori and rop derived from pBR322. Refer the Experimental Procedures of Iida, K. et al. J. Biol. Chem., 282 (35): 25659-25667, 2007. PMID: 17569661.
Vector backbone pBC111 (plasmid)
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae).
Depositor|Developer Iida, Hidetoshi |
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            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Cho, T. et al., Genes Cells 22(1), 94-104, 2017).
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            RDB15296 pBC111 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            original Iida, K., Essential, completely conserved glycine residue in the domain III S2-S3 linker of voltage-gated calcium channel alpha1 subunits in yeast and mammals. J. Biol. Chem. 282 (35): 25659-25667 (2007). PMID 17569661.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Iida, K., Essential, completely conserved glycine residue in the domain III S2-S3 linker of voltage-gated calcium channel alpha1 subunits in yeast and mammals. J. Biol. Chem. 282 (35): 25659-25667 (2007). PMID 17569661.

            2022.05.17

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