Resource data sheet
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pBC111 (#RDB15296)

S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli.

Clone info. S. cerevisiae-E. coli shuttle vector, replicating with a low copy number in E. coli, delivative of the YCplac111.
Comment The ColE1 ori of YCplac111 have been replaced with the ori and rop derived from pBR322. Refer the Experimental Procedures of Iida, K. et al. J. Biol. Chem., 282 (35): 25659-25667, 2007. PMID: 17569661.
Vector backbone pBC111 (plasmid)
Selectable markers Amp^r (E. coli), LEU2 (S. cerevisiae).
Depositor|Developer Iida, Hidetoshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Cho, T. et al., Genes Cells 22(1), 94-104, 2017).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cho, T. et al., Genes Cells 22(1), 94-104, 2017)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15296 pBC111 Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pBC111 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15296).

Reference section:

Iida, K., Teng, J., Tada, T., Saka, A., Tamai, M., Izumi-Nakaseko, H., Adachi-Akahane, S., Iida, H., Essential, completely conserved glycine residue in the domain III S2-S3 linker of voltage-gated calcium channel alpha1 subunits in yeast and mammals. J. Biol. Chem. 282 (35): 25659-25667 (2007). PMID 17569661. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Iida, K., Essential, completely conserved glycine residue in the domain III S2-S3 linker of voltage-gated calcium channel alpha1 subunits in yeast and mammals. J. Biol. Chem. 282 (35): 25659-25667 (2007). PMID 17569661. [link to RRC of NBRP]