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pFN18A-HP1a-Chromo-Halo (#RDB15211)

Expression vector of Drosophila Heterochromatin protein 1a (HP1a), Chromo domain

Clone info. Expression vector of Drosophila truncated form of Heterochromatin protein 1a (HP1a) [Chromo domain, 24 to 72 a.a], tagged with Halo, see Fig. 4A of Vo, N. et al. Nucleic Acids Res., 45 (3): 1233-1254, 2017.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pFN18A Flexi (plasmid)
Selectable markers Amp^r
Gene/insert name Drosophila dHP1 cDNA
Depositor|Developer Yamaguchi, Masamitsu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017).
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017)。

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RDB15211 pFN18A-HP1a-Chromo-Halo DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFN18A-HP1a-Chromo-Halo was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15211).

Reference section:

Vo, N., Anh Suong, D.N., Yoshino, N., Yoshida, H., Cotterill, S., Yamaguchi, M., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15211_A8Ksp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Halo-C_F ()
Sequence file: RDB15211_A8Ksa.seq check
>D04544B1_A8Ks_1_Halo-C_F_F05_17_ABI24.ab1
    1 GGGCGGTGAC CTGCTGGCAG GAGACAACCC GGACCTGATC GGCAGCGAGA TCGCGCGCTG
   61 GCTGTCGACG CTCGAGATTT CCGGCGAGCC AACCACTGAG GATCTGTACT TTCAGAGCGA
  121 TAACGCGATC GCCTACGCCG TGGAAAAGAT CATCGACAGG CGGGTGCGCA AGGGAAAGGT
  181 GGAGTACTAT CTGAAATGGA AGGGCTATCC CGAAACTGAG AACACGTGGG AGCCGGAGAA
  241 CAATCTCGAC TGCCAGGATC TTATCCAGCA GTACGAGGCG GTTTAAACGA ATTCGGGCTC
  301 GGTACCCGGG GATCCTCTAG AGTCGACCTG CAGGCATGCA AGCTGATCCG GCTGCTAACA
  361 AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC
  421 TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGTT
  481 CGCTTGCTGT CCATAAAACC GCCCAGTCTA GCTATCGCCA TGTAAGCCCA CTGCAAGCTA
  541 CCTGCTTTCT CTTTGCGCTT GCGTTTTCCC TTGTCCAGAT AGCCCAGTAG CTGACATTCA
  601 TCCGGGGTCA GCACCGTTTC TGCGGACTGG CTTTCTACGT AATGGTTTCT TAGACGTCAG
  661 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
  721 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
  781 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
  841 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
  901 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
  961 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTCAA AGTTCTGCTA TGTGGCGCGG
 1021 GTAATTATCC CGTATTTGAC GCCGGGGCAA GAGCAACTCG GTCGCCGCAT ACACTAATTC
 1081 TCAGATGACT TTGGTTGAGT ACTCACAGTT CACAGGAAAG CATCTTACTG GATGGTCAAT
1141 GACAAGTTAA GAAGAAATTA TATTG
//
Primer: rrnBT1ter_F ()
Sequence file: RDB15211_A8Ksb.seq check
>D04544B1_A8Ks_1_rrnBT1ter_F_D01_04_ABI08.ab1
    1 AGGGGGCGAG GATTGACGGT TGCGAAGCAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC
   61 CATAAACTGC CAGGCATCAA ATTAAGCAGA AGGCCATCCT GACGGATGGC CTTTTTGCGT
  121 TTCTACAAAC TCTTTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGGAT
  181 CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGTATT TTAACTTTAC TAAGGAGAAT
  241 TCATCATGGC AGAAATCGGT ACTGGCTTTC CATTCGACCC CCATTATGTG GAAGTCCTGG
  301 GCGAGCGCAT GCACTACGTC GATGTTGGTC CGCGCGATGG CACCCCTGTG CTGTTCCTGC
  361 ACGGTAACCC GACCTCCTCC TACGTGTGGC GCAACATCAT CCCGCATGTT GCACCGACCC
  421 ATCGCTGCAT TGCTCCAGAC CTGATCGGTA TGGGCAAATC CGACAAACCA GACCTGGGTT
  481 ATTTCTTCGA CGACCACGTC CGCTTCATGG ATGCCTTCAT CGAAGCCCTG GGTCTGGAAG
  541 AGGTCGTCCT GGTCATTCAC GACTGGGGCT CCGCTCTGGG TTTCCACTGG GCCAAGCGCA
  601 ATCCAGAGCG CGTCAAAGGT ATTGCATTTA TGGAGTTCAT CCGCCCTATC CCGACCTGGG
  661 ACGAATGGCC AGAATTTGCC CGCGAGACCT TCCAGGCCTT CCGCACCACC GACGTCGGCC
  721 GCAAGCTGAT CATCGATCAG AACGTTTTTA TCGAGGGTAC GCTGCCGATG GGTGTCGTCC
  781 GCCCGCTGAC TGAAGTCGAG ATGGACCATT ACCGCGAGCC GTTCCTGAAT CCTGTTGACC
  841 GCGAGCCACT GTGGCGCTTC CCAAACGAGC TGCCAATCGC CGGTGAGCCA GCGAACATCG
  901 TCGCGCTGGT CGAAGAAATA CATGGAACTG GGCTGCACCA GTCCCCTGTC CCGAAGCTGC
  961 TGTTTCTGGG GGCACCCCAG GCGTTCTGAT CCCACCGGCC CGAAGCCCGC TCGCTGGACC
 1021 AAAAGCCCTG CCTAACCTGC AAGGCTGTGG AACATCGGAC CGGGATCTGA ATCTGCCTGC
 1081 CAAGAGAACC ACCCTGGACC TGATCGGCAG CGAGAATCGC GCGGCGCCTG GGGCCTGATT
1141 CCGAATG
//
Primer: pTrcHis-R ()
Sequence file: RDB15211_A8Ksc.seq check
>D04544B1_A8Ks_1_pTrcHiS-R_G05_20_ABI24.ab1
    1 AAGGCGGAAG ATCTCGATCC TCTACGCCGG ACGCATCGTG GCCGGAGTAC TGCGGTTTAT
   61 CTCGATGGCT ACGAGGGCAG ACAGTAAGTG GATTTACCAT AATCCCTTAA TTGTACGCAC
  121 CGCTAAAACG CGTTCAGCGC GATCACGGCA GCAGACAGGT AAAAATGGCA ACAAACCACC
  181 CGAAAAACTG CCGCAATCGC GCCTGATAAA TTTTAACCGT ATGAATACCT ATGCAACCAG
  241 AGGGTACAGG CCACATTACC CCCACTTAAT CCACTGAAGC TGCCATTTTT CATGGTTTCA
  301 CCATCCCAGC GAAGGGCCAT CCAGCGTGCG TTCCTGTATT TCCGGACTAT AAAAGGATCT
  361 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC
  421 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAAATCCT TTTTTTCTGC
  481 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG
  541 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA
  601 ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC
  661 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT
  721 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA
  781 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC
  841 TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC
  901 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGGAAACGCC
  961 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTTGTG
 1021 ATGCTCGTCA GGGGGGCGGG AGCCTATGGG AAAAACGCCA GCCAACGCGG GCCTTTTTTA
 1081 CGGATCTGAC TTTTGCTGAC TTTTGGCTCA CATGATCTTC TTGCCGATAT CCCCTTGATT
1141 TCTGGGTGGA ATACTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289. [link to RRC of NBRP]