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pFN18A-HP1a-Halo

Expression vector of Drosophila Heterochromatin protein 1a (HP1a)

Catalog number RDB15210
Resource name pFN18A-HP1a-Halo
Clone info. Expression vector of Drosophila Heterochromatin protein 1a (HP1a) [full length, 1 to 206 a.a], tagged with Halo, see Fig. 4A of Vo, N. et al. Nucleic Acids Res., 45 (3): 1233-1254, 2017.
Expression was confirmed by the depositor with western blotting.
Vector backbone pFN18A Flexi (plasmid)
Selectable markers Amp^r
Gene/insert name Drosophila dHP1 cDNA
Depositor Yamaguchi, Masamitsu |
 
Other clones in our bank Drosophila Su(var)205 (KEGG orthology K11587) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017).
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RDB15210 pFN18A-HP1a-Halo DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15210_A8Ksp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Halo-C_F RDB15210_A8Ksa.seq
>D04544A9_A8Ks_1_Halo-C_F_B05_05_ABI24.ab1
    1 GGGCTTACTG CTGCAGAGAC ACCCGGACCT GATCGGCAGC GAGATCGCGC GCTGGCTGTC
   61 GACGCTCGAG ATTTCCGGCG AGCCAACCAC TGAGGATCTG TACTTTCAGA GCGATAACGC
  121 GATCGCCGGC AAGAAAATCG ACAACCCTGA GAGCTCGGCA AAGGTTTCCG ATGCCGAAGA
  181 GGAGGAGGAG GAGTACGCCG TGGAAAAGAT CATCGACAGG CGGGTGCGCA AGGGAAAGGT
  241 GGAGTACTAT CTGAAATGGA AGGGCTATCC CGAAACTGAG AACACGTGGG AGCCGGAGAA
  301 CAATCTCGAC TGCCAGGATC TTATCCAGCA GTACGAGGCG AGCCGCAAGG ATGAGGAGAA
  361 GTCAGCCGCC TCCAAGAAGG ATCGACCCAG CAGCAGCGCC AAGGCCAAGG AAACTCAAGG
  421 ACGCGCCAGC AGTTCGACGT CCACAGCAAG CAAGCGAAAG TCCGAAGAAC CAACAGCGCC
  481 CTCTGGCAAT AAATCAAAAC GTACCACAGA TGCGGAGCAG GACACCATTC CCGTTTCAGG
  541 ATCTACCGGA TTCGATCGCG GCCTGGAGGC CGAAAAGATC TTGGGTGCCT CCGACAATAA
  601 TGGCCGCCTG ACATTCCTCA TTCAGTTCAA AGGCGTGGAC CAAGCAGAAA TGGTGCCCTC
  661 CTCAGTGGCC AATGAAAAAA TCCCACGAAT GGTAATCCAC TTCTACGAAG AGCGCCTATC
  721 CTGGTACTCT GATAATGAAG ATGTTTAAAC GAATTCGGGC TCGGTACCCG GGGATCCTCT
  781 AGAGTCGACC TGCAGGCATG CAAGCTGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG
  841 AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG
  901 TCTTGAGGGG TTTTTTTGCT GAAAGGAGGA ACTATATCCG GTTCGCTTGC TGTCCATAAA
  961 ACCGCCCAGT CTAGCTATCG CCATGTAAGC CCACTGCAAG CTACCTGCTT TCTCTTTGCG
 1021 CTTGCGTTTT TCCCTTGTCC AGATAGCCCA GTAGCTGACA TTCATCCCGG GGTCAGCACC
 1081 GTTTTCTGCG AACTGACTTT TCTACGTATG ATTTCTTAGA CGGTTCAAGG TTGGCCACCT
1141 TTTTTTG
//
Primer: rrnBT1ter_F RDB15210_A8Ksb.seq
>D04544A9_A8Ks_1_rrnBT1ter_F_B01_02_ABI08.ab1
    1 CGGGGCGGAT TGACGTTGCG AGCACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA
   61 ACTGCCAGGC ATCAAATTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA
  121 CAAACTCTTT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGGATCTCGA
  181 TCCCGCGAAA TTAATACGAC TCACTATAGG GTATTTTAAC TTTACTAAGG AGAATTCATC
  241 ATGGCAGAAA TCGGTACTGG CTTTCCATTC GACCCCCATT ATGTGGAAGT CCTGGGCGAG
  301 CGCATGCACT ACGTCGATGT TGGTCCGCGC GATGGCACCC CTGTGCTGTT CCTGCACGGT
  361 AACCCGACCT CCTCCTACGT GTGGCGCAAC ATCATCCCGC ATGTTGCACC GACCCATCGC
  421 TGCATTGCTC CAGACCTGAT CGGTATGGGC AAATCCGACA AACCAGACCT GGGTTATTTC
  481 TTCGACGACC ACGTCCGCTT CATGGATGCC TTCATCGAAG CCCTGGGTCT GGAAGAGGTC
  541 GTCCTGGTCA TTCACGACTG GGGCTCCGCT CTGGGTTTCC ACTGGGCCAA GCGCAATCCA
  601 GAGCGCGTCA AAGGTATTGC ATTTATGGAG TTCATCCGCC CTATCCCGAC CTGGGACGAA
  661 TGGCCAGAAT TTGCCCGCGA GACCTTCCAG GCCTTCCGCA CCACCGACGT CGGCCGCAAG
  721 CTGATCATCG ATCAGAACGT TTTTATCGAG GGTACGCTGC CGATGGGTGT CGTCCGCCCG
  781 CTGACTGAAG TCGAGATGGA CCATTACCGC GAGCCGTTCC TGAATCCTGT TGACCGCGAG
  841 CCACTGTGGC GCTTCCCAAA CGAGCTGCCA ATCGCCGGTG AGCCAGCGAA CATCGTCGCG
  901 CTGGTCGAAG AATACATGGA CTGGCTGCAC CAGTCCCCTG TCCCGAAGCT GCTGTTTCTG
  961 GGGGCACCCC AGGCGTTCTG ATCCCACCCG GCCGAAGCCC GCTCGCCTGG CCAAAAGCCC
 1021 TGCCTTAACT TGCAAAGGCT GTGACATCGG CCCCGGGGTC CTGGATCCTG GCCTGGCAGG
1081 AGACCAGCTG GACCTGAATC GCAAGCGAAA TTCGCCGCGC TGGTCTGTTT CGAACGGCT
//
Primer: pTrcHis-R RDB15210_A8Ksc.seq
>D04544A9_A8Ks_1_pTrcHiS-R_D05_11_ABI24.ab1
    1 AAGGCCGGGA AAAATCTCGA TCCTCTACGC CGGACGCATC GTGGCCGGAG TACTGCGGTT
   61 TATCTCGATG GCTACGAGGG CAGACAGTAA GTGGATTTAC CATAATCCCT TAATTGTACG
  121 CACCGCTAAA ACGCGTTCAG CGCGATCACG GCAGCAGACA GGTAAAAATG GCAACAAACC
  181 ACCCGAAAAA CTGCCGCAAT CGCGCCTGAT AAATTTTAAC CGTATGAATA CCTATGCAAC
  241 CAGAGGGTAC AGGCCACATT ACCCCCACTT AATCCACTGA AGCTGCCATT TTTCATGGTT
  301 TCACCATCCC AGCGAAGGGC CATCCAGCGT GCGTTCCTGT ATTTCCGGAC TATAAAAGGA
  361 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
  421 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAAAT CCTTTTTTTC
  481 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
  541 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
  601 CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
  661 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
  721 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
  781 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
  841 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
  901 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
  961 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
 1021 GATGCTCGTC AGGGGGGCGG AGCCCTATGG AAAAACGCCA GCAACGCGGG CCTTTTTACC
 1081 GGATCTTGCC TTTTGGCTGG GCTTTTGCTC ACATGATCTT TCTGCGTATC CCTGGAATCC
1141 GTGATACGTA TACCGTCCTT GTTGCAAT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289.

2019.06.11

GNP_filter3_RDBDEP_html_190430.pl