Sequencing and PCR primers for primer information.
Expression vector of Drosophila Minichromosome maintenance protein 10 (MCM10), Linker 2
| Catalog number | RDB15208 |
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| Resource name | pFN18A-MCM10-Linker2-Halo |
| Clone info. | Expression vector of Drosophila truncated form of Minichromosome maintenance protein 10 (MCM10) [Linker 2, 431 to 510 a.a], tagged with Halo, see Fig. 4A of Vo, N. et al. Nucleic Acids Res., 45 (3): 1233-1254, 2017. Expression was confirmed by the depositor with western blotting. |
| Vector backbone | pFN18A Flexi (plasmid) |
| Selectable markers | Amp^r |
| Gene/insert name | Drosophila Mcm10 cDNA |
| Depositor | Yamaguchi, Masamitsu | |
| Other clones in our bank | Drosophila Mcm10 (KEGG orthology K10736) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017). |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15208 | pFN18A-MCM10-Linker2-Halo | DNA solution |
Electronic file
Original reference
| original | Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289. |
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Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15208_A8Ksp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: Halo-C_F | RDB15208_A8Ksa.seq |
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>D04544A7_A8Ks_3_Halo-C_F_B04_04_ABI24.ab1 1 AGGCCTGACC TGCTGCAGAG ACACCCGGAC CTGATCGGCA GCGAGATCGC GCGCTGGCTG 61 TCGACGCTCG AGATTTCCGG CGAGCCAACC ACTGAGGATC TGTACTTTCA GAGCGATAAC 121 GCGATCGCCC ATACCGCTAA CCACACCTCA AAGCCCAAAA CAGCCGAACC CACTAAAATT 181 CCATATGCAG AACGTGGCGG TCCTGTTTCC CGTTTGGCTG GTGGTGTGGA AGCGTCTAGG 241 AAACAGAGAG TCCAAGATCT AGAGCGGTTG CGTCTGCTTA AAGAGGAAAA TGAGCGCTTT 301 GAAAAAAAGA AGCAGGCGGA GGGCCATGTC TTGGGAAGTG ATAACAAAAA AGAATCTGAA 361 GCAGGCACAG TTTAAACGAA TTCGGGCTCG GTACCCGGGG ATCCTCTAGA GTCGACCTGC 421 AGGCATGCAA GCTGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC 481 CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT 541 TTTGCTGAAA GGAGGAACTA TATCCGGTTC GCTTGCTGTC CATAAAACCG CCCAGTCTAG 601 CTATCGCCAT GTAAGCCCAC TGCAAGCTAC CTGCTTTCTC TTTGCGCTTG CGTTTTCCCT 661 TGTCCAGATA GCCCAGTAGC TGACATTCAT CCGGGGTCAG CACCGTTTCT GCGGACTGGC 721 TTTCTACGTA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 781 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 841 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 901 CGCCCTTATT CCCTTTTTTT GCGGCATTTT GCCTTTCCTG TTTTTTGCTC ACCCAGAAAC 961 GCTGGGTGAA AGTAAAAGAT GCTGAAGATC AGTTTGGGTG CACGAGTGGG TTTACATCGA 1021 ACTGGATCTC AACAGCGGGT AAGATCCTTG AGAGTTTTCG CTCTCGGAAG AACGGTTTTC 1081 GATGATGAGC ACTTTTCAAA GTCTGCTATG TGGCGCGATA TAATCCCGGT ATTGACGCCC 1141 GGGCCAGAG // |
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| Primer: T7_-20bp | RDB15208_A8Ksb.seq |
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>D04544A7_A8Ks_3_T7_20pb_A04_01_ABI24.ab1 1 TCAATAGGGG GGAATTCATC ATGGCAGAAA TCGGTACTGG CTTTCCATTC GACCCCCATT 61 ATGTGGAAGT CCTGGGCGAG CGCATGCACT ACGTCGATGT TGGTCCGCGC GATGGCACCC 121 CTGTGCTGTT CCTGCACGGT AACCCGACCT CCTCCTACGT GTGGCGCAAC ATCATCCCGC 181 ATGTTGCACC GACCCATCGC TGCATTGCTC CAGACCTGAT CGGTATGGGC AAATCCGACA 241 AACCAGACCT GGGTTATTTC TTCGACGACC ACGTCCGCTT CATGGATGCC TTCATCGAAG 301 CCCTGGGTCT GGAAGAGGTC GTCCTGGTCA TTCACGACTG GGGCTCCGCT CTGGGTTTCC 361 ACTGGGCCAA GCGCAATCCA GAGCGCGTCA AAGGTATTGC ATTTATGGAG TTCATCCGCC 421 CTATCCCGAC CTGGGACGAA TGGCCAGAAT TTGCCCGCGA GACCTTCCAG GCCTTCCGCA 481 CCACCGACGT CGGCCGCAAG CTGATCATCG ATCAGAACGT TTTTATCGAG GGTACGCTGC 541 CGATGGGTGT CGTCCGCCCG CTGACTGAAG TCGAGATGGA CCATTACCGC GAGCCGTTCC 601 TGAATCCTGT TGACCGCGAG CCACTGTGGC GCTTCCCAAA CGAGCTGCCA ATCGCCGGTG 661 AGCCAGCGAA CATCGTCGCG CTGGTCGAAG AATACATGGA CTGGCTGCAC CAGTCCCCTG 721 TCCCGAAGCT GCTGTTCTGG GGCACCCCAG GCGTTCTGAT CCCACCGGCC GAAGCCGCTC 781 GCCTGGCCAA AAGCCTGCCT AACTGCAAGG CTGTGGACAT CGGCCCGGGT CTGAATCTGC 841 TGCAAGAAGA CAACCCGGAC CTGATCGGCA GCGAGATCGC GCGCTGGCTG TCGACGCTCG 901 AGATTTCCGG CGAGCCAACC ACTGAGGATC TGTACTTTCA GAGCGATAAC GCGATCGCCC 961 ATACCGCTAA CCACACCTCA AGCCCAAAAC AGCCGAACCC ACTAAAATTT CCATATGCAG 1021 AACGTGGCGG GTCCCTGTTT CCCGTTTGGC TGGGTGGTGT GGAAAGCGTC TAGAAACAGA 1081 GAGTTCCAGA TCTAGAGCCG ATGCCGTCCT GCCTAAGAGG AGAATGGAGG CGGCTTTTTG 1141 AATAAAAAG // |
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| Primer: pTrcHiS-R | RDB15208_A8Ksc.seq |
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>D04544A7_A8Ks_3_pTrcHiS-R_D04_10_ABI24.ab1 1 AGGGGCCTGA AAGATCTCGG ATCCTCTACG CCGGACGCAT CGTGGCCGGA GTACTGCGGT 61 TTATCTCGAT GGCTACGAGG GCAGACAGTA AGTGGATTTA CCATAATCCC TTAATTGTAC 121 GCACCGCTAA AACGCGTTCA GCGCGATCAC GGCAGCAGAC AGGTAAAAAT GGCAACAAAC 181 CACCCGAAAA ACTGCCGCAA TCGCGCCTGA TAAATTTTAA CCGTATGAAT ACCTATGCAA 241 CCAGAGGGTA CAGGCCACAT TACCCCCACT TAATCCACTG AAGCTGCCAT TTTTCATGGT 301 TTCACCATCC CAGCGAAGGG CCATCCAGCG TGCGTTCCTG TATTTCCGGA CTATAAAAGG 361 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG 421 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAAA TCCTTTTTTT 481 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG 541 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA 601 CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA 661 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG 721 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC 781 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA 841 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG 901 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC 961 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG 1021 TGATGCTCGT CAGGGGGGCG GGAGCCCTAT GGGAAAACGC CAGCACGGCG GCCTTTTTAC 1081 CGGATCTGAC TTTTGCTGGA CTTTTGCTCA CATGATCTTT CCTGCGTTTA ATTCCCCTGA 1141 TCTGTTGAAT TAACCGTAAT TTAACG // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289. |
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2019.07.19
GNP_filter3_RDBDEP_html_190432.pl