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pFN18A-MCM10-Linker2-Halo

Expression vector of Drosophila Minichromosome maintenance protein 10 (MCM10), Linker 2

Catalog number RDB15208
Resource name pFN18A-MCM10-Linker2-Halo
Clone info. Expression vector of Drosophila truncated form of Minichromosome maintenance protein 10 (MCM10) [Linker 2, 431 to 510 a.a], tagged with Halo, see Fig. 4A of Vo, N. et al. Nucleic Acids Res., 45 (3): 1233-1254, 2017.
Expression was confirmed by the depositor with western blotting.
Vector backbone pFN18A Flexi (plasmid)
Selectable markers Amp^r
Gene/insert name Drosophila Mcm10 cDNA
Depositor Yamaguchi, Masamitsu |
 
Other clones in our bank Drosophila Mcm10 (KEGG orthology K10736) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017).
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RDB15208 pFN18A-MCM10-Linker2-Halo DNA solution

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References and tips

Electronic file

Original reference

original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15208_A8Ksp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Halo-C_F RDB15208_A8Ksa.seq
>D04544A7_A8Ks_3_Halo-C_F_B04_04_ABI24.ab1
    1 AGGCCTGACC TGCTGCAGAG ACACCCGGAC CTGATCGGCA GCGAGATCGC GCGCTGGCTG
   61 TCGACGCTCG AGATTTCCGG CGAGCCAACC ACTGAGGATC TGTACTTTCA GAGCGATAAC
  121 GCGATCGCCC ATACCGCTAA CCACACCTCA AAGCCCAAAA CAGCCGAACC CACTAAAATT
  181 CCATATGCAG AACGTGGCGG TCCTGTTTCC CGTTTGGCTG GTGGTGTGGA AGCGTCTAGG
  241 AAACAGAGAG TCCAAGATCT AGAGCGGTTG CGTCTGCTTA AAGAGGAAAA TGAGCGCTTT
  301 GAAAAAAAGA AGCAGGCGGA GGGCCATGTC TTGGGAAGTG ATAACAAAAA AGAATCTGAA
  361 GCAGGCACAG TTTAAACGAA TTCGGGCTCG GTACCCGGGG ATCCTCTAGA GTCGACCTGC
  421 AGGCATGCAA GCTGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC
  481 CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT
  541 TTTGCTGAAA GGAGGAACTA TATCCGGTTC GCTTGCTGTC CATAAAACCG CCCAGTCTAG
  601 CTATCGCCAT GTAAGCCCAC TGCAAGCTAC CTGCTTTCTC TTTGCGCTTG CGTTTTCCCT
  661 TGTCCAGATA GCCCAGTAGC TGACATTCAT CCGGGGTCAG CACCGTTTCT GCGGACTGGC
  721 TTTCTACGTA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC
  781 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC
  841 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT
  901 CGCCCTTATT CCCTTTTTTT GCGGCATTTT GCCTTTCCTG TTTTTTGCTC ACCCAGAAAC
  961 GCTGGGTGAA AGTAAAAGAT GCTGAAGATC AGTTTGGGTG CACGAGTGGG TTTACATCGA
 1021 ACTGGATCTC AACAGCGGGT AAGATCCTTG AGAGTTTTCG CTCTCGGAAG AACGGTTTTC
 1081 GATGATGAGC ACTTTTCAAA GTCTGCTATG TGGCGCGATA TAATCCCGGT ATTGACGCCC
1141 GGGCCAGAG
//
Primer: T7_-20bp RDB15208_A8Ksb.seq
>D04544A7_A8Ks_3_T7_20pb_A04_01_ABI24.ab1
    1 TCAATAGGGG GGAATTCATC ATGGCAGAAA TCGGTACTGG CTTTCCATTC GACCCCCATT
   61 ATGTGGAAGT CCTGGGCGAG CGCATGCACT ACGTCGATGT TGGTCCGCGC GATGGCACCC
  121 CTGTGCTGTT CCTGCACGGT AACCCGACCT CCTCCTACGT GTGGCGCAAC ATCATCCCGC
  181 ATGTTGCACC GACCCATCGC TGCATTGCTC CAGACCTGAT CGGTATGGGC AAATCCGACA
  241 AACCAGACCT GGGTTATTTC TTCGACGACC ACGTCCGCTT CATGGATGCC TTCATCGAAG
  301 CCCTGGGTCT GGAAGAGGTC GTCCTGGTCA TTCACGACTG GGGCTCCGCT CTGGGTTTCC
  361 ACTGGGCCAA GCGCAATCCA GAGCGCGTCA AAGGTATTGC ATTTATGGAG TTCATCCGCC
  421 CTATCCCGAC CTGGGACGAA TGGCCAGAAT TTGCCCGCGA GACCTTCCAG GCCTTCCGCA
  481 CCACCGACGT CGGCCGCAAG CTGATCATCG ATCAGAACGT TTTTATCGAG GGTACGCTGC
  541 CGATGGGTGT CGTCCGCCCG CTGACTGAAG TCGAGATGGA CCATTACCGC GAGCCGTTCC
  601 TGAATCCTGT TGACCGCGAG CCACTGTGGC GCTTCCCAAA CGAGCTGCCA ATCGCCGGTG
  661 AGCCAGCGAA CATCGTCGCG CTGGTCGAAG AATACATGGA CTGGCTGCAC CAGTCCCCTG
  721 TCCCGAAGCT GCTGTTCTGG GGCACCCCAG GCGTTCTGAT CCCACCGGCC GAAGCCGCTC
  781 GCCTGGCCAA AAGCCTGCCT AACTGCAAGG CTGTGGACAT CGGCCCGGGT CTGAATCTGC
  841 TGCAAGAAGA CAACCCGGAC CTGATCGGCA GCGAGATCGC GCGCTGGCTG TCGACGCTCG
  901 AGATTTCCGG CGAGCCAACC ACTGAGGATC TGTACTTTCA GAGCGATAAC GCGATCGCCC
  961 ATACCGCTAA CCACACCTCA AGCCCAAAAC AGCCGAACCC ACTAAAATTT CCATATGCAG
 1021 AACGTGGCGG GTCCCTGTTT CCCGTTTGGC TGGGTGGTGT GGAAAGCGTC TAGAAACAGA
 1081 GAGTTCCAGA TCTAGAGCCG ATGCCGTCCT GCCTAAGAGG AGAATGGAGG CGGCTTTTTG
1141 AATAAAAAG
//
Primer: pTrcHiS-R RDB15208_A8Ksc.seq
>D04544A7_A8Ks_3_pTrcHiS-R_D04_10_ABI24.ab1
    1 AGGGGCCTGA AAGATCTCGG ATCCTCTACG CCGGACGCAT CGTGGCCGGA GTACTGCGGT
   61 TTATCTCGAT GGCTACGAGG GCAGACAGTA AGTGGATTTA CCATAATCCC TTAATTGTAC
  121 GCACCGCTAA AACGCGTTCA GCGCGATCAC GGCAGCAGAC AGGTAAAAAT GGCAACAAAC
  181 CACCCGAAAA ACTGCCGCAA TCGCGCCTGA TAAATTTTAA CCGTATGAAT ACCTATGCAA
  241 CCAGAGGGTA CAGGCCACAT TACCCCCACT TAATCCACTG AAGCTGCCAT TTTTCATGGT
  301 TTCACCATCC CAGCGAAGGG CCATCCAGCG TGCGTTCCTG TATTTCCGGA CTATAAAAGG
  361 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG
  421 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAAA TCCTTTTTTT
  481 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG
  541 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA
  601 CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA
  661 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG
  721 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC
  781 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA
  841 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG
  901 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC
  961 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG
 1021 TGATGCTCGT CAGGGGGGCG GGAGCCCTAT GGGAAAACGC CAGCACGGCG GCCTTTTTAC
 1081 CGGATCTGAC TTTTGCTGGA CTTTTGCTCA CATGATCTTT CCTGCGTTTA ATTCCCCTGA
1141 TCTGTTGAAT TAACCGTAAT TTAACG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289.

2019.07.19

GNP_filter3_RDBDEP_html_190432.pl