Sequencing and PCR primers for primer information.
Expression vector of Drosophila Minichromosome maintenance protein 10 (MCM10), Internal Domain
| Catalog number | RDB15207 |
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| Resource name | pFN18A-MCM10-ID-term-Halo |
| Clone info. | Expression vector of Drosophila truncated form of Minichromosome maintenance protein 10 (MCM10) [Internal Domain, 241 to 430 a.a], tagged with Halo, see Fig. 4A of Vo, N. et al. Nucleic Acids Res., 45 (3): 1233-1254, 2017. Expression was confirmed by the depositor with western blotting. |
| Vector backbone | pFN18A Flexi (plasmid) |
| Selectable markers | Amp^r |
| Gene/insert name | Drosophila Mcm10 cDNA |
| Depositor | Yamaguchi, Masamitsu | |
| Other clones in our bank | Drosophila Mcm10 (KEGG orthology K10736) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017). |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15207 | pFN18A-MCM10-ID-term-Halo | DNA solution |
Electronic file
Original reference
| original | Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289. |
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Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15207_A8Ksp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: Halo-C_F | RDB15207_A8Ksa.seq |
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>D04544A6_A8Ks_2_Halo-C_F_F03_18_ABI24.ab1 1 GCCTTACTGC TGCAGAGACA CCCGGACCTG ATCGGCAGCG AGATCGCGCG CTGGCTGTCG 61 ACGCTCGAGA TTTCCGGCGA GCCAACCACT GAGGATCTGT ACTTTCAGAG CGATAACGCG 121 ATCGCCGTTT CCAAAAATCC TGTAAAAAAC ACCAAGAAGG GTGATCCCTA CTCCACGTGG 181 AAACTATCCG ATCTACGGGG AGAGGTTAAA ACGATCTCAC TTTTCCTTTT TAAAGAGGCC 241 CACAAATCCC TGTGGAAAAC AGCGGAGGGT CTGTGCTTGG CTGTGTTGAA TCCAACTATT 301 TTCGAGAGGA GAGCGGGAAG CTCCGATGTG GCCTGCCTAT CCATCGATAG CTCCCAGAAA 361 GTCATGATCC TGGGTCAATC CAAAGATTTG GGCACATGTC GGGCCACCAA AAAAAATGGG 421 GACAAGTGCA CTTCGGTGGT TAACCTAACC GACTGTGATT ATTGCATTTT TCATGTAAAG 481 CAGGAATATG GCAAGATGTC CCGACGTTCT GAACTGCAAT CGGCGACCGC AGGTCGTGGT 541 ATCAATGAAC TAAGAAACAA GGTTTTGGGC AAAAACGAGG TATTTTACGG CGGCCAAACA 601 TTTACTGCAG TTCCCGCAAG AAAAAGTGCC AAGTTAATCA CCAAGGAACG TGATCGTCTG 661 AGTATGCTGG CTGGCTATGA TGTTTCCCCC TTCGCCGTTT AAACGAATTC GGGCTCGGTA 721 CCCGGGGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT GATCCGGCTG CTAACAAAGC 781 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG 841 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGTTCGCT 901 TGCTGTCCAT AAAACCGCCC AGTCTAGCTA TCGCCATGTA AGCCCACTGC AAGCTACCTG 961 CTTTCTCTTT GCGCTTGCGT TTTCCCTTTG TCCAGATAGC CCAGTAGCTG ACATTCATCC 1021 CGGGGTCAGC ACCGTTTCTG CGGACTGGCT TTCTACGTAA TGGCTTTCTT AGACGTCAGT 1081 GGCACTTTTT CGGGGAAATG TGCGCGACCC TAATTGATTA TTTTTCTAAT AACATTCCAA 1141 AATATG // |
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| Primer: T7 terminator | RDB15207_A8Ksb.seq |
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>D04544A6_A8Ks_2_T7 terminator_G03_21_ABI24.ab1 1 GATAGCTCTT TCGGGCTTTG TTAGCAGCCG GATCAGCTTG CATGCCTGCA GGTCGACTCT 61 AGAGGATCCC CGGGTACCGA GCCCGAATTC GTTTAAACGG CGAAGGGGGA AACATCATAG 121 CCAGCCAGCA TACTCAGACG ATCACGTTCC TTGGTGATTA ACTTGGCACT TTTTCTTGCG 181 GGAACTGCAG TAAATGTTTG GCCGCCGTAA AATACCTCGT TTTTGCCCAA AACCTTGTTT 241 CTTAGTTCAT TGATACCACG ACCTGCGGTC GCCGATTGCA GTTCAGAACG TCGGGACATC 301 TTGCCATATT CCTGCTTTAC ATGAAAAATG CAATAATCAC AGTCGGTTAG GTTAACCACC 361 GAAGTGCACT TGTCCCCATT TTTTTTGGTG GCCCGACATG TGCCCAAATC TTTGGATTGA 421 CCCAGGATCA TGACTTTCTG GGAGCTATCG ATGGATAGGC AGGCCACATC GGAGCTTCCC 481 GCTCTCCTCT CGAAAATAGT TGGATTCAAC ACAGCCAAGC ACAGACCCTC CGCTGTTTTC 541 CACAGGGATT TGTGGGCCTC TTTAAAAAGG AAAAGTGAGA TCGTTTTAAC CTCTCCCCGT 601 AGATCGGATA GTTTCCACGT GGAGTAGGGA TCACCCTTCT TGGTGTTTTT TACAGGATTT 661 TTGGAAACGG CGATCGCGTT ATCGCTCTGA AAGTACAGAT CCTCAGTGGT TGGCTCGCCG 721 GAAATCTCGA GCGTCGACAG CCAGCGCGCG ATCTCGCTGC CGATCAGGTC CGGGTTGTCT 781 TCTTGCAGCA GATTCAGACC CGGGCCGATG TCCACAGCCT TGCAGTTAGG CAGGCTTTTG 841 GCCAGGCGAG CGGCTTCGGC CGGTGGGATC AGAACGCCTG GGGGTGCCCC AGAACAGCAG 901 CTTCGGGACA GGGGACTGGT GCAGCCAGTC CATGTATTCT TCGACCAGCG CGACGATGTT 961 CGCTGGCTCA CCGGCGATTG GCAGCTCGTT TGGGAAGCGC CACAGTGGCT CGCGGTCAAC 1021 AGGATTCAGA ACGGCTCGCG GTAATGGTCC ATCTCGACTT CAGTCAGCGG GCGACGAACA 1081 CCCAATCGGG CAGCGTTACC GATCGATTAG ACGGATCCTT GGATCGAATG GAATCAAGCT 1141 TGGCGGACCG GAACCGTTCC GAGT // |
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| Primer: T7_-20bp | RDB15207_A8Ksc.seq |
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>D04544A6_A8Ks_2_T7_20pb_E03_15_ABI24.ab1 1 TAATACGAGA TCATCATGGC AGAAATCGGT ACTGGCTTTC CATTCGACCC CCATTATGTG 61 GAAGTCCTGG GCGAGCGCAT GCACTACGTC GATGTTGGTC CGCGCGATGG CACCCCTGTG 121 CTGTTCCTGC ACGGTAACCC GACCTCCTCC TACGTGTGGC GCAACATCAT CCCGCATGTT 181 GCACCGACCC ATCGCTGCAT TGCTCCAGAC CTGATCGGTA TGGGCAAATC CGACAAACCA 241 GACCTGGGTT ATTTCTTCGA CGACCACGTC CGCTTCATGG ATGCCTTCAT CGAAGCCCTG 301 GGTCTGGAAG AGGTCGTCCT GGTCATTCAC GACTGGGGCT CCGCTCTGGG TTTCCACTGG 361 GCCAAGCGCA ATCCAGAGCG CGTCAAAGGT ATTGCATTTA TGGAGTTCAT CCGCCCTATC 421 CCGACCTGGG ACGAATGGCC AGAATTTGCC CGCGAGACCT TCCAGGCCTT CCGCACCACC 481 GACGTCGGCC GCAAGCTGAT CATCGATCAG AACGTTTTTA TCGAGGGTAC GCTGCCGATG 541 GGTGTCGTCC GCCCGCTGAC TGAAGTCGAG ATGGACCATT ACCGCGAGCC GTTCCTGAAT 601 CCTGTTGACC GCGAGCCACT GTGGCGCTTC CCAAACGAGC TGCCAATCGC CGGTGAGCCA 661 GCGAACATCG TCGCGCTGGT CGAAGAATAC ATGGACTGGC TGCACCAGTC CCCTGTCCCG 721 AAGCTGCTGT TCTGGGGCAC CCCAGGCGTT CTGATCCCAC CGGCCGAAGC CGCTCGCCTG 781 GCCAAAAGCC TGCCTAACTG CAAGGCTGTG GACATCGGCC CGGGTCTGAA TCTGCTGCAA 841 GAAGACAACC CGGACCTGAT CGGCAGCGAG ATCGCGCGCT GGCTGTCGAC GCTCGAGATT 901 TCCGGCGAGC CAACCACTGA GGATCTGTAC TTTCAGAGCG ATAACGCGAT CGCCGTTTCC 961 AAAAATCCTG TAAAAAACAC CAAGAAAGGG TGATCCCTAC TCCACGTGGA AACTATCCGA 1021 TCTACGGGGA GAGGTTAAAA CGATCTCACT TTTCCCTTTT TTAAAGAGCC CACAAATCCC 1081 TGTGGCAAAA CAGCGAAGGA TCTGTGCGTG ACCTGTGTTG AATCCGAACT TATTTTTTCC 1141 GA // |
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| Primer: pTrcHiS-R | RDB15207_A8Ksd.seq |
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>D04544A6_A8Ks_2_pTrcHiS-R_H03_24_ABI24.ab1 1 AAGGCGGAAA GATCTCGATC CTCTACGCCG GACGCATCGT GGCCGGAGTA CTGCGGTTTA 61 TCTCGATGGC TACGAGGGCA GACAGTAAGT GGATTTACCA TAATCCCTTA ATTGTACGCA 121 CCGCTAAAAC GCGTTCAGCG CGATCACGGC AGCAGACAGG TAAAAATGGC AACAAACCAC 181 CCGAAAAACT GCCGCAATCG CGCCTGATAA ATTTTAACCG TATGAATACC TATGCAACCA 241 GAGGGTACAG GCCACATTAC CCCCACTTAA TCCACTGAAG CTGCCATTTT TCATGGTTTC 301 ACCATCCCAG CGAAGGGCCA TCCAGCGTGC GTTCCTGTAT TTCCGGACTA TAAAAGGATC 361 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC 421 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAAATCC TTTTTTTCTG 481 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG 541 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA 601 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 661 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG 721 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA 781 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 841 CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 901 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGGAAACGC 961 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCCTCTG ACTTGAGCGT CGATTTTTGT 1021 GATGCTCGTC AGGGGGGCGG GAGCCTATGG GAAAAACGCC AGCAACGCGG GCCTTTTTTA 1081 CGGATCTGGC TTTTGCTGGC CTTTTGGCTC ACATGGACCT TTCTGCCGAT ATCCCCTTGA 1141 ATTCTGGTGG AATAGTG // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289. |
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2019.07.19
GNP_filter3_RDBDEP_html_190432.pl