Resource data sheet
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pFN18A-MCM10-ID-term-Halo

Expression vector of Drosophila Minichromosome maintenance protein 10 (MCM10), Internal Domain

Catalog number RDB15207
Resource name pFN18A-MCM10-ID-term-Halo
Clone info. Expression vector of Drosophila truncated form of Minichromosome maintenance protein 10 (MCM10) [Internal Domain, 241 to 430 a.a], tagged with Halo, see Fig. 4A of Vo, N. et al. Nucleic Acids Res., 45 (3): 1233-1254, 2017.
Expression was confirmed by the depositor with western blotting.
Vector backbone pFN18A Flexi (plasmid)
Selectable markers Amp^r
Gene/insert name Drosophila Mcm10 cDNA
Depositor Yamaguchi, Masamitsu |
 
Other clones in our bank Drosophila Mcm10 (KEGG orthology K10736) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017).
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RDB15207 pFN18A-MCM10-ID-term-Halo DNA solution

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References and tips

Electronic file

Original reference

original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15207_A8Ksp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Halo-C_F RDB15207_A8Ksa.seq
>D04544A6_A8Ks_2_Halo-C_F_F03_18_ABI24.ab1
    1 GCCTTACTGC TGCAGAGACA CCCGGACCTG ATCGGCAGCG AGATCGCGCG CTGGCTGTCG
   61 ACGCTCGAGA TTTCCGGCGA GCCAACCACT GAGGATCTGT ACTTTCAGAG CGATAACGCG
  121 ATCGCCGTTT CCAAAAATCC TGTAAAAAAC ACCAAGAAGG GTGATCCCTA CTCCACGTGG
  181 AAACTATCCG ATCTACGGGG AGAGGTTAAA ACGATCTCAC TTTTCCTTTT TAAAGAGGCC
  241 CACAAATCCC TGTGGAAAAC AGCGGAGGGT CTGTGCTTGG CTGTGTTGAA TCCAACTATT
  301 TTCGAGAGGA GAGCGGGAAG CTCCGATGTG GCCTGCCTAT CCATCGATAG CTCCCAGAAA
  361 GTCATGATCC TGGGTCAATC CAAAGATTTG GGCACATGTC GGGCCACCAA AAAAAATGGG
  421 GACAAGTGCA CTTCGGTGGT TAACCTAACC GACTGTGATT ATTGCATTTT TCATGTAAAG
  481 CAGGAATATG GCAAGATGTC CCGACGTTCT GAACTGCAAT CGGCGACCGC AGGTCGTGGT
  541 ATCAATGAAC TAAGAAACAA GGTTTTGGGC AAAAACGAGG TATTTTACGG CGGCCAAACA
  601 TTTACTGCAG TTCCCGCAAG AAAAAGTGCC AAGTTAATCA CCAAGGAACG TGATCGTCTG
  661 AGTATGCTGG CTGGCTATGA TGTTTCCCCC TTCGCCGTTT AAACGAATTC GGGCTCGGTA
  721 CCCGGGGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT GATCCGGCTG CTAACAAAGC
  781 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG
  841 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGTTCGCT
  901 TGCTGTCCAT AAAACCGCCC AGTCTAGCTA TCGCCATGTA AGCCCACTGC AAGCTACCTG
  961 CTTTCTCTTT GCGCTTGCGT TTTCCCTTTG TCCAGATAGC CCAGTAGCTG ACATTCATCC
 1021 CGGGGTCAGC ACCGTTTCTG CGGACTGGCT TTCTACGTAA TGGCTTTCTT AGACGTCAGT
 1081 GGCACTTTTT CGGGGAAATG TGCGCGACCC TAATTGATTA TTTTTCTAAT AACATTCCAA
1141 AATATG
//
Primer: T7 terminator RDB15207_A8Ksb.seq
>D04544A6_A8Ks_2_T7 terminator_G03_21_ABI24.ab1
    1 GATAGCTCTT TCGGGCTTTG TTAGCAGCCG GATCAGCTTG CATGCCTGCA GGTCGACTCT
   61 AGAGGATCCC CGGGTACCGA GCCCGAATTC GTTTAAACGG CGAAGGGGGA AACATCATAG
  121 CCAGCCAGCA TACTCAGACG ATCACGTTCC TTGGTGATTA ACTTGGCACT TTTTCTTGCG
  181 GGAACTGCAG TAAATGTTTG GCCGCCGTAA AATACCTCGT TTTTGCCCAA AACCTTGTTT
  241 CTTAGTTCAT TGATACCACG ACCTGCGGTC GCCGATTGCA GTTCAGAACG TCGGGACATC
  301 TTGCCATATT CCTGCTTTAC ATGAAAAATG CAATAATCAC AGTCGGTTAG GTTAACCACC
  361 GAAGTGCACT TGTCCCCATT TTTTTTGGTG GCCCGACATG TGCCCAAATC TTTGGATTGA
  421 CCCAGGATCA TGACTTTCTG GGAGCTATCG ATGGATAGGC AGGCCACATC GGAGCTTCCC
  481 GCTCTCCTCT CGAAAATAGT TGGATTCAAC ACAGCCAAGC ACAGACCCTC CGCTGTTTTC
  541 CACAGGGATT TGTGGGCCTC TTTAAAAAGG AAAAGTGAGA TCGTTTTAAC CTCTCCCCGT
  601 AGATCGGATA GTTTCCACGT GGAGTAGGGA TCACCCTTCT TGGTGTTTTT TACAGGATTT
  661 TTGGAAACGG CGATCGCGTT ATCGCTCTGA AAGTACAGAT CCTCAGTGGT TGGCTCGCCG
  721 GAAATCTCGA GCGTCGACAG CCAGCGCGCG ATCTCGCTGC CGATCAGGTC CGGGTTGTCT
  781 TCTTGCAGCA GATTCAGACC CGGGCCGATG TCCACAGCCT TGCAGTTAGG CAGGCTTTTG
  841 GCCAGGCGAG CGGCTTCGGC CGGTGGGATC AGAACGCCTG GGGGTGCCCC AGAACAGCAG
  901 CTTCGGGACA GGGGACTGGT GCAGCCAGTC CATGTATTCT TCGACCAGCG CGACGATGTT
  961 CGCTGGCTCA CCGGCGATTG GCAGCTCGTT TGGGAAGCGC CACAGTGGCT CGCGGTCAAC
 1021 AGGATTCAGA ACGGCTCGCG GTAATGGTCC ATCTCGACTT CAGTCAGCGG GCGACGAACA
 1081 CCCAATCGGG CAGCGTTACC GATCGATTAG ACGGATCCTT GGATCGAATG GAATCAAGCT
1141 TGGCGGACCG GAACCGTTCC GAGT
//
Primer: T7_-20bp RDB15207_A8Ksc.seq
>D04544A6_A8Ks_2_T7_20pb_E03_15_ABI24.ab1
    1 TAATACGAGA TCATCATGGC AGAAATCGGT ACTGGCTTTC CATTCGACCC CCATTATGTG
   61 GAAGTCCTGG GCGAGCGCAT GCACTACGTC GATGTTGGTC CGCGCGATGG CACCCCTGTG
  121 CTGTTCCTGC ACGGTAACCC GACCTCCTCC TACGTGTGGC GCAACATCAT CCCGCATGTT
  181 GCACCGACCC ATCGCTGCAT TGCTCCAGAC CTGATCGGTA TGGGCAAATC CGACAAACCA
  241 GACCTGGGTT ATTTCTTCGA CGACCACGTC CGCTTCATGG ATGCCTTCAT CGAAGCCCTG
  301 GGTCTGGAAG AGGTCGTCCT GGTCATTCAC GACTGGGGCT CCGCTCTGGG TTTCCACTGG
  361 GCCAAGCGCA ATCCAGAGCG CGTCAAAGGT ATTGCATTTA TGGAGTTCAT CCGCCCTATC
  421 CCGACCTGGG ACGAATGGCC AGAATTTGCC CGCGAGACCT TCCAGGCCTT CCGCACCACC
  481 GACGTCGGCC GCAAGCTGAT CATCGATCAG AACGTTTTTA TCGAGGGTAC GCTGCCGATG
  541 GGTGTCGTCC GCCCGCTGAC TGAAGTCGAG ATGGACCATT ACCGCGAGCC GTTCCTGAAT
  601 CCTGTTGACC GCGAGCCACT GTGGCGCTTC CCAAACGAGC TGCCAATCGC CGGTGAGCCA
  661 GCGAACATCG TCGCGCTGGT CGAAGAATAC ATGGACTGGC TGCACCAGTC CCCTGTCCCG
  721 AAGCTGCTGT TCTGGGGCAC CCCAGGCGTT CTGATCCCAC CGGCCGAAGC CGCTCGCCTG
  781 GCCAAAAGCC TGCCTAACTG CAAGGCTGTG GACATCGGCC CGGGTCTGAA TCTGCTGCAA
  841 GAAGACAACC CGGACCTGAT CGGCAGCGAG ATCGCGCGCT GGCTGTCGAC GCTCGAGATT
  901 TCCGGCGAGC CAACCACTGA GGATCTGTAC TTTCAGAGCG ATAACGCGAT CGCCGTTTCC
  961 AAAAATCCTG TAAAAAACAC CAAGAAAGGG TGATCCCTAC TCCACGTGGA AACTATCCGA
 1021 TCTACGGGGA GAGGTTAAAA CGATCTCACT TTTCCCTTTT TTAAAGAGCC CACAAATCCC
 1081 TGTGGCAAAA CAGCGAAGGA TCTGTGCGTG ACCTGTGTTG AATCCGAACT TATTTTTTCC
1141 GA
//
Primer: pTrcHiS-R RDB15207_A8Ksd.seq
>D04544A6_A8Ks_2_pTrcHiS-R_H03_24_ABI24.ab1
    1 AAGGCGGAAA GATCTCGATC CTCTACGCCG GACGCATCGT GGCCGGAGTA CTGCGGTTTA
   61 TCTCGATGGC TACGAGGGCA GACAGTAAGT GGATTTACCA TAATCCCTTA ATTGTACGCA
  121 CCGCTAAAAC GCGTTCAGCG CGATCACGGC AGCAGACAGG TAAAAATGGC AACAAACCAC
  181 CCGAAAAACT GCCGCAATCG CGCCTGATAA ATTTTAACCG TATGAATACC TATGCAACCA
  241 GAGGGTACAG GCCACATTAC CCCCACTTAA TCCACTGAAG CTGCCATTTT TCATGGTTTC
  301 ACCATCCCAG CGAAGGGCCA TCCAGCGTGC GTTCCTGTAT TTCCGGACTA TAAAAGGATC
  361 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC
  421 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAAATCC TTTTTTTCTG
  481 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG
  541 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA
  601 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG
  661 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG
  721 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA
  781 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC
  841 CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT
  901 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGGAAACGC
  961 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCCTCTG ACTTGAGCGT CGATTTTTGT
 1021 GATGCTCGTC AGGGGGGCGG GAGCCTATGG GAAAAACGCC AGCAACGCGG GCCTTTTTTA
 1081 CGGATCTGGC TTTTGCTGGC CTTTTGGCTC ACATGGACCT TTCTGCCGAT ATCCCCTTGA
1141 ATTCTGGTGG AATAGTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289.

2019.07.19

GNP_filter3_RDBDEP_html_190432.pl