Resource data sheet
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pFN18A-MCM10-ID-term-Halo (#RDB15207)

Expression vector of Drosophila Minichromosome maintenance protein 10 (MCM10), Internal Domain

Clone info. Expression vector of Drosophila truncated form of Minichromosome maintenance protein 10 (MCM10) [Internal Domain, 241 to 430 a.a], tagged with Halo, see Fig. 4A of Vo, N. et al. Nucleic Acids Res., 45 (3): 1233-1254, 2017.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pFN18A Flexi (plasmid)
Selectable markers Amp^r
Gene/insert name Drosophila Mcm10 cDNA
Depositor|Developer Yamaguchi, Masamitsu |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15207 pFN18A-MCM10-ID-term-Halo DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFN18A-MCM10-ID-term-Halo was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15207).

Reference section:

Vo, N., Anh Suong, D.N., Yoshino, N., Yoshida, H., Cotterill, S., Yamaguchi, M., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15207_A8Ksp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Halo-C_F ()
Sequence file: RDB15207_A8Ksa.seq check
1
Primer: T7 terminator ()
Sequence file: RDB15207_A8Ksb.seq check
1
Primer: T7_-20bp ()
Sequence file: RDB15207_A8Ksc.seq check
1
Primer: pTrcHiS-R ()
Sequence file: RDB15207_A8Ksd.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Vo, N., Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res. 45 (3): 1233-1254 (2017). PMID 28180289. [link to RRC of NBRP]