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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mCherry-LC3B

Autophagosome marker. Expression vector of rat Microtubule-associated proteins 1A/1B light chain 3B (LC3B), mCherry tag.

Catalog number RDB15159
Resource name mCherry-LC3B
Clone info. Expression vector of rat Microtubule-associated proteins 1A/1B light chain 3B (LC3B) with N-terminally tagged mCherry. Autophagosome marker.
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy.
Vector backbone pmCherry-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Map1lc3b cDNA
Depositor|Developer Hamachi, Itaru |
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External Database
rat Map1lc3b

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Miki T. et al., Nat. Methods 13 (11): 931-937, 2016).
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          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
          Remarks
          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miki T. et al., Nat. Methods 13 (11): 931-937, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
          備考
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15159 mCherry-LC3B DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Autophagy (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15159_A7Flp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: mCherry-C_F
          Region: mCherry,insert,SV40p(A),f1ori
          Sequence file: RDB15159_A7Fla.seq check
          >D04425A8_A7Fl_2_mCherry_C_F_H11_08_ABI08.ab1
              1 TGACACGCCA GGCCCGTGCA GCTGCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA
             61 CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT
            121 CCACCGGCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGCT CAAGCTTCGA
            181 ATTCCATGCC GTCCGAGAAG ACCTTCAAAC AGCGCCGGAG CTTCGAACAA AGAGTGGAAG
            241 ATGTCCGGCT CATCCGGGAG CAGCACCCCA CCAAGATCCC AGTGATTATA GAGCGATACA
            301 AGGGTGAGAA GCAGCTGCCC GTCCTGGACA AGACCAAGTT CCTTGTACCT GATCACGTGA
            361 ATATGAGCGA ACTCATCAAG ATAATTAGAA GGCGCCTGCA GCTCAATGCT AACCAAGCCT
            421 TCTTCCTCCT GGTGAATGGG CACAGCATGG TGAGTGTGTC CACACCCATC TCTGAAGTGT
            481 ACGAGAGCGA GAGAGATGAA GACGGCTTCC TGTACATGGT CTATGCCTCC CAGGAGACGT
            541 TCGGGACAGC ACTGGCTGTG TAAGGATCCA CCGGATCTAG ATAACTGATC ATAATCAGCC
            601 ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC
            661 TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT
            721 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA
            781 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAACGCGT AAATTGTAAG CGTTAATATT
            841 TTGTTAAAAT TCGCGTTAAA TTTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA
            901 AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC
            961 AGTTTGGAAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CCAACGTCAA AGGGCGAAAA
           1021 ACCGTCTATC AGCGATGCCA CTACGTGAAC CCATCACCCT TATTCAGTTT TTTTGGGGGT
          1081 CCAGGTGCCG TAAGGCACTT AATCCGGGAG CCTTAAAGGG AGTCTTC
          //
          Primer: CMV_Forward
          Region: CMVpro,mCherry,insert5'
          Sequence file: RDB15159_A7Flb.seq check
          >15159_15159_A7Fl_CMV-Forward_A07_01_ABI24.ab1
              1 CCTTCACTCC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
            121 GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA
            181 TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT
            241 ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT
            301 GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG
            361 ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA
            421 ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT
            481 TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA
            541 AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA
            601 AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA
            661 AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA
            721 AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG
            781 AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG
            841 CTCAAGCTTC GAATTCCATG CCGTCCGAGA AGACCTTCAA ACAGCGCCGG AGCTTCGAAC
            901 AAAGAGTGGA AAGATGTCCG GCTCATCCGG GAGCAGCACC CCACCAAGAT CCCAGTGATT
            961 ATAGAGCGAT ACAAGGGTGA GAAGCAGCTG CCCGTCCTGA CAGACCCAAG TTCCCTTGTA
           1021 CCTGATCACG TGAATATGAG CGAACTCATC CAGGATTATA GAGCGCCTGC AGCTCAATGC
          1081 CTAACAAGCT TTCATCGTTC CCTGGGGTGA ATTGGGCCCA CAG
          //
          Primer: SV40pro_F_V2
          Region: SV40pro_ori,NeoR_KanR
          Sequence file: RDB15159_A7Flc.seq check
          >15159_15159_A7Fl_SV40pro_F_V2_B07_04_ABI24.ab1
              1 GTTACGACTT TTTTGGAGGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
             61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
            601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
            721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
            781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
            841 GCCTTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT TCGAAATGAC CCGACCAAGC
            901 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
            961 CTTCGGAATT CGTTTTCCGG GGACGCCCGG CTTGGATGAT CCTTCCAGCG CGGGATTCTT
           1021 CATGCTGGGA GGTTCTTTCG GCCACCCTTA GGGGGAGCTT AACCTGAACA CGCAAGGAGA
          1081 CAATACGGAG ACCGCGCTAT TGACGGCCAA ATTAAAAAAG GAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391.
          user_report Sakaguchi, H., NEAT1 Confers Radioresistance to Hepatocellular Carcinoma Cells by Inducing Autophagy through GABARAP. Int. J. Mol. Sci. 23 (2): 711 (2022). PMID 35054896.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl