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mCherry-LC3B (#RDB15159)

Autophagosome marker. Expression vector of rat Microtubule-associated proteins 1A/1B light chain 3B (LC3B), mCherry tag.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Miki, T., Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of rat Microtubule-associated proteins 1A/1B light chain 3B (LC3B) with N-terminally tagged mCherry. Autophagosome marker.
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy.
Vector backbone pmCherry-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Growth conditions LB+Kanamycin, 37oC
Gene/insert name rat Map1lc3b cDNA
Depositor|Developer Hamachi, Itaru |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Miki T. et al., Nat. Methods 13 (11): 931-937, 2016).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miki T. et al., Nat. Methods 13 (11): 931-937, 2016)。

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RDB15159 mCherry-LC3B DNA solution

How to cite this biological resource

Materials & Methods section:

The mCherry-LC3B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15159).

Reference section:

Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [PubMed] [Article] [RRC of NBRP]
user_report Sakaguchi, H., NEAT1 Confers Radioresistance to Hepatocellular Carcinoma Cells by Inducing Autophagy through GABARAP. Int. J. Mol. Sci. 23 (2): 711 (2022). PMID 35054896. [PubMed] [Article] [RRC of NBRP]