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mCherry-LC3B (#RDB15159)

Autophagosome marker. Expression vector of rat Microtubule-associated proteins 1A/1B light chain 3B (LC3B), mCherry tag.

Clone info. Expression vector of rat Microtubule-associated proteins 1A/1B light chain 3B (LC3B) with N-terminally tagged mCherry. Autophagosome marker.
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy.
Vector backbone pmCherry-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Map1lc3b cDNA
Depositor|Developer Hamachi, Itaru |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Miki T. et al., Nat. Methods 13 (11): 931-937, 2016).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miki T. et al., Nat. Methods 13 (11): 931-937, 2016)。

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RDB15159 mCherry-LC3B DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mCherry-LC3B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15159).

Reference section:

Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15159_A7Flp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F (Pr0453)
Region: mCherry,insert,SV40p(A),f1ori
Sequence file: RDB15159_A7Fla.seq check
>D04425A8_A7Fl_2_mCherry_C_F_H11_08_ABI08.ab1
    1 TGACACGCCA GGCCCGTGCA GCTGCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA
   61 CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT
  121 CCACCGGCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGCT CAAGCTTCGA
  181 ATTCCATGCC GTCCGAGAAG ACCTTCAAAC AGCGCCGGAG CTTCGAACAA AGAGTGGAAG
  241 ATGTCCGGCT CATCCGGGAG CAGCACCCCA CCAAGATCCC AGTGATTATA GAGCGATACA
  301 AGGGTGAGAA GCAGCTGCCC GTCCTGGACA AGACCAAGTT CCTTGTACCT GATCACGTGA
  361 ATATGAGCGA ACTCATCAAG ATAATTAGAA GGCGCCTGCA GCTCAATGCT AACCAAGCCT
  421 TCTTCCTCCT GGTGAATGGG CACAGCATGG TGAGTGTGTC CACACCCATC TCTGAAGTGT
  481 ACGAGAGCGA GAGAGATGAA GACGGCTTCC TGTACATGGT CTATGCCTCC CAGGAGACGT
  541 TCGGGACAGC ACTGGCTGTG TAAGGATCCA CCGGATCTAG ATAACTGATC ATAATCAGCC
  601 ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC
  661 TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT
  721 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA
  781 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAACGCGT AAATTGTAAG CGTTAATATT
  841 TTGTTAAAAT TCGCGTTAAA TTTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA
  901 AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC
  961 AGTTTGGAAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CCAACGTCAA AGGGCGAAAA
 1021 ACCGTCTATC AGCGATGCCA CTACGTGAAC CCATCACCCT TATTCAGTTT TTTTGGGGGT
1081 CCAGGTGCCG TAAGGCACTT AATCCGGGAG CCTTAAAGGG AGTCTTC
//
Primer: CMV_Forward (Pr0016)
Region: CMVpro,mCherry,insert5'
Sequence file: RDB15159_A7Flb.seq check
>15159_15159_A7Fl_CMV-Forward_A07_01_ABI24.ab1
    1 CCTTCACTCC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
  121 GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA
  181 TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT
  241 ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT
  301 GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG
  361 ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA
  421 ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT
  481 TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA
  541 AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA
  601 AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA
  661 AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA
  721 AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG
  781 AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG
  841 CTCAAGCTTC GAATTCCATG CCGTCCGAGA AGACCTTCAA ACAGCGCCGG AGCTTCGAAC
  901 AAAGAGTGGA AAGATGTCCG GCTCATCCGG GAGCAGCACC CCACCAAGAT CCCAGTGATT
  961 ATAGAGCGAT ACAAGGGTGA GAAGCAGCTG CCCGTCCTGA CAGACCCAAG TTCCCTTGTA
 1021 CCTGATCACG TGAATATGAG CGAACTCATC CAGGATTATA GAGCGCCTGC AGCTCAATGC
1081 CTAACAAGCT TTCATCGTTC CCTGGGGTGA ATTGGGCCCA CAG
//
Primer: SV40pro_F_V2 (Pr0154)
Region: SV40pro_ori,NeoR_KanR
Sequence file: RDB15159_A7Flc.seq check
>15159_15159_A7Fl_SV40pro_F_V2_B07_04_ABI24.ab1
    1 GTTACGACTT TTTTGGAGGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  841 GCCTTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT TCGAAATGAC CCGACCAAGC
  901 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
  961 CTTCGGAATT CGTTTTCCGG GGACGCCCGG CTTGGATGAT CCTTCCAGCG CGGGATTCTT
 1021 CATGCTGGGA GGTTCTTTCG GCCACCCTTA GGGGGAGCTT AACCTGAACA CGCAAGGAGA
1081 CAATACGGAG ACCGCGCTAT TGACGGCCAA ATTAAAAAAG GAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]
user_report Sakaguchi, H., NEAT1 Confers Radioresistance to Hepatocellular Carcinoma Cells by Inducing Autophagy through GABARAP. Int. J. Mol. Sci. 23 (2): 711 (2022). PMID 35054896. [link to RRC of NBRP]