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mCherry-RAB1A

Expression vector of rat Ras-related protein Rab-1A (RAB1A), mCherry tag.

Catalog number RDB15158
Resource name mCherry-RAB1A
Clone info. Expression vector of rat Ras-related protein Rab-1A (RAB1A) with N-terminally tagged mCherry.
Expression was confirmed by the depositor with confocal lase scanning microscopy.
Vector backbone pmCherry-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Rab1a cDNA
Depositor Hamachi, Itaru |
 
Other clones in our bank rat Rab1a (KEGG orthology K07874) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
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RDB15158 mCherry-RAB1A DNA solution

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References and tips

Electronic file

Featured content

References

original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15158_A7Flp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F RDB15158_A7Fla.seq
>D04425A7_A7Fl_1_mCherry-C_F_B10_04_ABI24.ab1
    1 TGACCAGAAA GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA
   61 CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT
  121 CCACCGGCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGCT ATGAATCCCG
  181 AATATGATTA TTTATTCAAG TTACTCCTGA TTGGCGACTC TGGGGTTGGA AAGTCTTGCC
  241 TTCTCCTTAG GTTTGCGGAT GACACGTATA CGGAAAGCTA CATCAGCACG ATTGGTGTGG
  301 ATTTCAAGAT ACGGACTATA GAGCTAGACG GGAAAACAAT CAAGCTTCAG ATATGGGACA
  361 CAGCAGGCCA GGAAAGATTT CGAACAATCA CCTCCAGTTA TTACAGAGGA GCCCATGGCA
  421 TCATAGTTGT GTATGATGTG ACAGACCAGG AGTCCTTCAA TAACGTGAAA CAGTGGCTGC
  481 AGGAGATCGA TCGCTACGCC AGCGAAAATG TCAACAAGTT GTTGGTAGGG AACAAATGTG
  541 ACCTGACCAC AAAGAAAGTA GTAGACTACA CAACAGCCAA GGAATTTGCA GATTCCCTTG
  601 GAATTCCATT TTTGGAAACC AGTGCTAAGA ACGCAACGAA TGTAGAACAG TCTTTCATGA
  661 CCATGGCAGC GGAGATTAAA AAGCGGATGG GTCCTGGAGC AACAGCTGGA GGTGCGGAGA
  721 AGTCCAATGT TAAAATCCAG AGCACTCCAG TCAAGCAGTC AGGTGGAGGC TGCTGCTAAG
  781 GATCCACCGG ATCTAGATAA CTGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC
  841 TTGCTTTAAA AAAACCTCCC ACACCTCCCC CCTGAACCTG AAACATAAAA TGAATGCAAT
  901 TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
  961 AAATTTCACA AATAAAGCAT TTTTTTTCAC TGCATTCTAG TTTGTGGGTT TGTCCCAAAC
 1021 TCATCAATGT ATCTTTAACG CGTAAATTTG TAAGCGTTTA ATATTTTGTT AAAATCGCGT
1081 CAAATTTTTA TTAAATCAGG CCCTTCCAAT TTGTA
//
Primer: CMV-Forward RDB15158_A7Flb.seq
>D04425A7_A7Fl_1_CMV-Forward_A10_01_ABI24.ab1
    1 CGATACACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
  121 GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA
  181 TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT
  241 ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT
  301 GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG
  361 ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA
  421 ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT
  481 TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA
  541 AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA
  601 AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA
  661 AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA
  721 AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG
  781 AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG
  841 CTATGAATCC CGAATATGAT TATTTATTCA AGTTACTCCT GATTGGCGAC TCTGGGGTTG
  901 GAAAGTCTTG CCTTCTCCTT AGGTTTGCGG ATGACACGTA TACGGAAAGC TACATCAGGC
  961 ACGATTGGTG TGGGATTTCA AGATACGGAC TATAGAGCTA GACGCAAAAC AATCAGCTTT
 1021 CAGATATGGG ACACAGCAGT CAGCAAAGGA TTTCGGAACA ATCACTTCAG TATACAGAGG
1081 AGCTCAATGC ATCAAATAAG GTTGGGTGG
//
Primer: SV40pro_F_V2 RDB15158_A7Flc.seq
>D04425A7_A7Fl_1_SV40prp_F_V2_G11_07_ABI08.ab1
    1 GAAGGGGTTT TTTTGGGAGG CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA
  901 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CCTTCTATGA AAGGTTGGGC
  961 TTCGGAATCG TTTTTCCGGG ACGCCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
 1021 TGGAGTTCTT TCGCCACCCT ACGGGGAGCC TACCTGAAAC ACGGAAAGGA GACATTACTG
1081 GAAGGAACCC GCCGCTATGA ACGGCCCAAT AGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391.

2018.11.21

GNP_filter3_RDBDEP_html_181012.pl