Please review the QC test results indicated by icon below as well as clone information before placing your order.

mCherry-RAB1A

Expression vector of rat Ras-related protein Rab-1A (RAB1A), mCherry tag.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Methods 13 (11): 931-937, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15158_A7Flp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F Sequence file: RDB15158_A7Fla.seq
>D04425A7_A7Fl_1_mCherry-C_F_B10_04_ABI24.ab1     1 TGACCAGAAA GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA    61 CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT   121 CCACCGGCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGCT ATGAATCCCG   181 AATATGATTA TTTATTCAAG TTACTCCTGA TTGGCGACTC TGGGGTTGGA AAGTCTTGCC   241 TTCTCCTTAG GTTTGCGGAT GACACGTATA CGGAAAGCTA CATCAGCACG ATTGGTGTGG   301 ATTTCAAGAT ACGGACTATA GAGCTAGACG GGAAAACAAT CAAGCTTCAG ATATGGGACA   361 CAGCAGGCCA GGAAAGATTT CGAACAATCA CCTCCAGTTA TTACAGAGGA GCCCATGGCA   421 TCATAGTTGT GTATGATGTG ACAGACCAGG AGTCCTTCAA TAACGTGAAA CAGTGGCTGC   481 AGGAGATCGA TCGCTACGCC AGCGAAAATG TCAACAAGTT GTTGGTAGGG AACAAATGTG   541 ACCTGACCAC AAAGAAAGTA GTAGACTACA CAACAGCCAA GGAATTTGCA GATTCCCTTG   601 GAATTCCATT TTTGGAAACC AGTGCTAAGA ACGCAACGAA TGTAGAACAG TCTTTCATGA   661 CCATGGCAGC GGAGATTAAA AAGCGGATGG GTCCTGGAGC AACAGCTGGA GGTGCGGAGA   721 AGTCCAATGT TAAAATCCAG AGCACTCCAG TCAAGCAGTC AGGTGGAGGC TGCTGCTAAG   781 GATCCACCGG ATCTAGATAA CTGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC   841 TTGCTTTAAA AAAACCTCCC ACACCTCCCC CCTGAACCTG AAACATAAAA TGAATGCAAT   901 TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC   961 AAATTTCACA AATAAAGCAT TTTTTTTCAC TGCATTCTAG TTTGTGGGTT TGTCCCAAAC  1021 TCATCAATGT ATCTTTAACG CGTAAATTTG TAAGCGTTTA ATATTTTGTT AAAATCGCGT 1081 CAAATTTTTA TTAAATCAGG CCCTTCCAAT TTGTA //
Primer: CMV-Forward Sequence file: RDB15158_A7Flb.seq
>D04425A7_A7Fl_1_CMV-Forward_A10_01_ABI24.ab1     1 CGATACACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA   121 GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA   181 TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT   241 ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT   301 GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG   361 ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA   421 ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT   481 TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA   541 AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA   601 AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA   661 AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA   721 AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG   781 AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG   841 CTATGAATCC CGAATATGAT TATTTATTCA AGTTACTCCT GATTGGCGAC TCTGGGGTTG   901 GAAAGTCTTG CCTTCTCCTT AGGTTTGCGG ATGACACGTA TACGGAAAGC TACATCAGGC   961 ACGATTGGTG TGGGATTTCA AGATACGGAC TATAGAGCTA GACGCAAAAC AATCAGCTTT  1021 CAGATATGGG ACACAGCAGT CAGCAAAGGA TTTCGGAACA ATCACTTCAG TATACAGAGG 1081 AGCTCAATGC ATCAAATAAG GTTGGGTGG //
Primer: SV40pro_F_V2 Sequence file: RDB15158_A7Flc.seq
>D04425A7_A7Fl_1_SV40prp_F_V2_G11_07_ABI08.ab1     1 GAAGGGGTTT TTTTGGGAGG CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT    61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA   121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC   181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA   241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG   301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC   361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG   421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA   481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC   541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA   601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG   661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT   721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG   781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC   841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA   901 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CCTTCTATGA AAGGTTGGGC   961 TTCGGAATCG TTTTTCCGGG ACGCCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC  1021 TGGAGTTCTT TCGCCACCCT ACGGGGAGCC TACCTGAAAC ACGGAAAGGA GACATTACTG 1081 GAAGGAACCC GCCGCTATGA ACGGCCCAAT AGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl