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ARF4-mCherry (#RDB15156)

Expression vector of rat ADP-ribosylation factor 4 (ARF4), mCherry fused tag.

Clone info. Expression vector of rat ADP-ribosylation factor 4 (ARF4) with C-terminally tagged mCherry fused.
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy.
Vector backbone pmCherry-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Arf4 cDNA
Depositor|Developer Hamachi, Itaru |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
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RDB15156 ARF4-mCherry DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The ARF4-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15156).

Reference section:

Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15156_A7Flp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15156_A7Fla.seq check
>D04425A5_A7Fl_1_CMV-Forward_H08_23_ABI24.ab1
    1 CTTCCACTCC GCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGC
  121 CACCATGGGC CTCACCATCT CCTCTCTCTT CTCGCGCCTC TTCGGCAAGA AGCAGATGCG
  181 CATTTTGATG GTTGGATTGG ATGCTGCGGG CAAGACGACG ATTCTGTATA AACTGAAACT
  241 AGGGGAGATA GTCACCACCA TTCCTACCAT TGGTTTTAAT GTGGAAACAG TAGAATATAA
  301 GAACATTTGT TTCACAGTTT GGGATGTTGG TGGTCAAGAT AAAATTAGGC CTCTCTGGAG
  361 GCATTACTTC CAGAATACCC AGGGTCTCAT TTTCGTGGTA GATAGCAATG ATCGTGAAAG
  421 AATTCAGGAA GGAGCAGCCG TGCTGCAGAA AATGCTTCTG GAAGATGAGC TGCAGGATGC
  481 TGTGCTGCTG CTTTTTGCCA ACAAACAGGA TTTGCCAAAT GCTATGGCCA TCAGTGAGAT
  541 GACAGACAAG TTAGGTCTTC AGTCTCTCCG GAACAGAACA TGGTATGTCC AAGCCACTTG
  601 TGCTACACAA GGAACTGGTC TGTACGAGGG GCTGGATTGG CTGTCAAATG AACTTTCAAA
  661 ACGCGGGGAT CCACCGGTCG CCACCATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCAT
  721 CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG GCCACGAGTT
  781 CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA
  841 GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC AGTTCATGTA
  901 CGGCTCCAAG GCCTACGTGA AGCACCCCCG CCGACATCCC CGACTACTTG AAGCTGTCCC
  961 TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGGACGGCG GGCGTGGTGA
 1021 CCGTGACCCA GGACTCGTCC CTGCAAGACC GCCGAGTCCA TCCTACATGT GAGCTTGCGG
1081 CGGCCACCGA ACCTTCCCCC TCCCGGACCG GATC
//
Primer: SV40polyA-R
Sequence file: RDB15156_A7Flb.seq check
>D04425A5_A7Fl_1_SV40polyA-R_A09_03_ABI24.ab1
    1 AAAAGGGGGA GTTTTTAAGC AAGTAAACCT CTACAAATGT GGTATGGCTG ATTATGATCT
   61 AGAGTCGCGG CCGCTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG CGGCCCTCGG
  121 CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAGGTGATG TCCAACTTGA
  181 TGTTGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG GTGGTCTTGA
  241 CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC TCGCCCTTCA
  301 GGGCGCCGTC CTCGGGGTAC ATCCGCTCGG AGGAGGCCTC CCAGCCCATG GTCTTCTTCT
  361 GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG CTTCACCTTG TAGATGAACT
  421 CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA CGGTCACCAC GCCGCCGTCC TCGAAGTTCA
  481 TCACGCGCTC CCACTTGAAG CCCTCGGGGA AGGACAGCTT CAAGTAGTCG GGGATGTCGG
  541 CGGGGTGCTT CACGTAGGCC TTGGAGCCGT ACATGAACTG AGGGGACAGG ATGTCCCAGG
  601 CGAAGGGCAG GGGGCCACCC TTGGTCACCT TCAGCTTGGC GGTCTGGGTG CCCTCGTAGG
  661 GGCGGCCCTC GCCCTCGCCC TCGATCTCGA ACTCGTGGCC GTTCACGGAG CCCTCCATGT
  721 GCACCTTGAA GCGCATGAAC TCCTTGATGA TGGCCATGTT ATCCTCCTCG CCCTTGCTCA
  781 CCATGGTGGC GACCGGTGGA TCCCCGCGTT TTGAAAGTTC ATTTGACAGC CAATCCAGCC
  841 CCTCGTACAG ACCAGTTCCT TGTGTAGCAC AAGTGGCTTG GACATACCAT GTTCTGTTCC
  901 GGAGAGACTG AAGACCTAAC TTGTCTGTCA TCTCACTGAT GGCCATAGCA TTTTGGCAAA
  961 TCCTGTTTGT TGGCAAAAAG CAGCAGCACA GCATCCCTGC AGCTCATCTT CCAGGAAGGC
 1021 ATTTTTTCTG CAGCAACGGC TGCTTCCCTT CCTGAATTTC TTTTCACGAT CATGGCTAAT
1081 CTACCACGAC ATGGAGACTC TTTGGGGGTA TTTTCTTGGA AG
//
Primer: SV40pro_F_V2
Sequence file: RDB15156_A7Flc.seq check
>D04425A5_A7Fl_1_SV40pro_F_V2_B09_06_ABI24.ab1
    1 GGCAAACTTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA
   61 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC
  601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA
  901 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CCTTCTATGA AAGGTTGGGG
  961 CTTCGGAATC GTTTTTCCCG GGGACGCCCG GCTGGGATGA TCCCTCCCAG CGCGGGGATC
 1021 TCATGGCTGG AGTTCTTTCG CCCACCCTTA AGGGGGAAGC TACTGAAACA CGAGGAGACC
1081 ATACCGGAAG AACCCGCGCC TTATGGGAAC G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]