Please review the QC test results indicated by icon below as well as clone information before placing your order.

ARF4-mCherry

Expression vector of rat ADP-ribosylation factor 4 (ARF4), mCherry fused tag.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Methods 13 (11): 931-937, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15156_A7Flp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB15156_A7Fla.seq
>D04425A5_A7Fl_1_CMV-Forward_H08_23_ABI24.ab1     1 CTTCCACTCC GCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGC   121 CACCATGGGC CTCACCATCT CCTCTCTCTT CTCGCGCCTC TTCGGCAAGA AGCAGATGCG   181 CATTTTGATG GTTGGATTGG ATGCTGCGGG CAAGACGACG ATTCTGTATA AACTGAAACT   241 AGGGGAGATA GTCACCACCA TTCCTACCAT TGGTTTTAAT GTGGAAACAG TAGAATATAA   301 GAACATTTGT TTCACAGTTT GGGATGTTGG TGGTCAAGAT AAAATTAGGC CTCTCTGGAG   361 GCATTACTTC CAGAATACCC AGGGTCTCAT TTTCGTGGTA GATAGCAATG ATCGTGAAAG   421 AATTCAGGAA GGAGCAGCCG TGCTGCAGAA AATGCTTCTG GAAGATGAGC TGCAGGATGC   481 TGTGCTGCTG CTTTTTGCCA ACAAACAGGA TTTGCCAAAT GCTATGGCCA TCAGTGAGAT   541 GACAGACAAG TTAGGTCTTC AGTCTCTCCG GAACAGAACA TGGTATGTCC AAGCCACTTG   601 TGCTACACAA GGAACTGGTC TGTACGAGGG GCTGGATTGG CTGTCAAATG AACTTTCAAA   661 ACGCGGGGAT CCACCGGTCG CCACCATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCAT   721 CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG GCCACGAGTT   781 CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA   841 GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC AGTTCATGTA   901 CGGCTCCAAG GCCTACGTGA AGCACCCCCG CCGACATCCC CGACTACTTG AAGCTGTCCC   961 TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGGACGGCG GGCGTGGTGA  1021 CCGTGACCCA GGACTCGTCC CTGCAAGACC GCCGAGTCCA TCCTACATGT GAGCTTGCGG 1081 CGGCCACCGA ACCTTCCCCC TCCCGGACCG GATC //
Primer: SV40polyA-R Sequence file: RDB15156_A7Flb.seq
>D04425A5_A7Fl_1_SV40polyA-R_A09_03_ABI24.ab1     1 AAAAGGGGGA GTTTTTAAGC AAGTAAACCT CTACAAATGT GGTATGGCTG ATTATGATCT    61 AGAGTCGCGG CCGCTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG CGGCCCTCGG   121 CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAGGTGATG TCCAACTTGA   181 TGTTGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG GTGGTCTTGA   241 CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC TCGCCCTTCA   301 GGGCGCCGTC CTCGGGGTAC ATCCGCTCGG AGGAGGCCTC CCAGCCCATG GTCTTCTTCT   361 GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG CTTCACCTTG TAGATGAACT   421 CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA CGGTCACCAC GCCGCCGTCC TCGAAGTTCA   481 TCACGCGCTC CCACTTGAAG CCCTCGGGGA AGGACAGCTT CAAGTAGTCG GGGATGTCGG   541 CGGGGTGCTT CACGTAGGCC TTGGAGCCGT ACATGAACTG AGGGGACAGG ATGTCCCAGG   601 CGAAGGGCAG GGGGCCACCC TTGGTCACCT TCAGCTTGGC GGTCTGGGTG CCCTCGTAGG   661 GGCGGCCCTC GCCCTCGCCC TCGATCTCGA ACTCGTGGCC GTTCACGGAG CCCTCCATGT   721 GCACCTTGAA GCGCATGAAC TCCTTGATGA TGGCCATGTT ATCCTCCTCG CCCTTGCTCA   781 CCATGGTGGC GACCGGTGGA TCCCCGCGTT TTGAAAGTTC ATTTGACAGC CAATCCAGCC   841 CCTCGTACAG ACCAGTTCCT TGTGTAGCAC AAGTGGCTTG GACATACCAT GTTCTGTTCC   901 GGAGAGACTG AAGACCTAAC TTGTCTGTCA TCTCACTGAT GGCCATAGCA TTTTGGCAAA   961 TCCTGTTTGT TGGCAAAAAG CAGCAGCACA GCATCCCTGC AGCTCATCTT CCAGGAAGGC  1021 ATTTTTTCTG CAGCAACGGC TGCTTCCCTT CCTGAATTTC TTTTCACGAT CATGGCTAAT 1081 CTACCACGAC ATGGAGACTC TTTGGGGGTA TTTTCTTGGA AG //
Primer: SV40pro_F_V2 Sequence file: RDB15156_A7Flc.seq
>D04425A5_A7Fl_1_SV40pro_F_V2_B09_06_ABI24.ab1     1 GGCAAACTTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA    61 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG   121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC   181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG   241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC   301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG   361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT   421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG   481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT   541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC   601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG   661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC   721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT   781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT   841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA   901 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CCTTCTATGA AAGGTTGGGG   961 CTTCGGAATC GTTTTTCCCG GGGACGCCCG GCTGGGATGA TCCCTCCCAG CGCGGGGATC  1021 TCATGGCTGG AGTTCTTTCG CCCACCCTTA AGGGGGAAGC TACTGAAACA CGAGGAGACC 1081 ATACCGGAAG AACCCGCGCC TTATGGGAAC G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl