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PDIA3-mCherry-KDEL (#RDB15153)

Expression vector of rat Protein disulfide-isomerase A3 (PDIA3), mCherry-ER retention signal (KDEL) tag.

Clone info. Expression vector of rat Protein disulfide-isomerase A3 (PDIA3) with C-terminally tagged mCherry-ER retention signal (KDEL). cDNA fragment 74nt to 1588nt of NM_017319.1 is cloned.
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy. Known differences (at least): C to T substitution at 1499nt of NM_017319.1 (Leu to Phe).
Vector backbone pmCherry-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Pdia3 cDNA
Depositor|Developer Hamachi, Itaru |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
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RDB15153 PDIA3-mCherry-KDEL DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The PDIA3-mCherry-KDEL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15153).

Reference section:

Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15153_A7Flp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15153_A7Fla.seq check
>D04425A2_A7Fl_2_CMV-Forward_E07_13_ABI24.ab1
    1 ACTTTCAAAC TCCCGCCCCC ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG ACTCAGATCT
  121 CGAGGCCACC ATGCGCTTCA GCTGCCTTGC GCTGCTCCCG GGCGTGGCGT TGCTGCTCGC
  181 CTCGGCCCTC CTCGCCTCCG CCTCAGACGT GTTGGAACTG ACGGACGAAA ACTTCGAGAG
  241 TCGCGTCTCC GACACGGGCT CAGCTGGGCT CATGCTAGTC GAGTTCTTCG CCCCATGGTG
  301 TGGACATTGC AAGAGGCTTG CCCCTGAGTA TGAAGCTGCA GCAACCAGAT TAAAAGGAAT
  361 AGTCCCATTA GCAAAGGTGG ACTGCACTGC CAACACAAAC ACCTGTAATA AGTATGGCGT
  421 CAGCGGCTAC CCAACTCTTA AAATATTTAG AGATGGTGAA GAAGCGGGTG CTTATGATGG
  481 GCCTAGGACT GCCGATGGAA TTGTCAGCCA CTTGAAGAAA CAAGCAGGAC CAGCTTCAGT
  541 TCCTCTCAGG ACTGAGGACG AATTTAAGAA GTTCATTAGT GATAAAGATG CCTCGGTGGT
  601 GGGCTTTTTC AGGGATTTAT TCAGTGATGG CCACTCCGAG TTCCTAAAAG CAGCCAGCAA
  661 CTTGAGAGAT AACTACCGAT TTGCACACAC CAACGTTGAA TCTCTGGTGA AGGAGTACGA
  721 TGATAATGGA GAGGGGATTA CTATATTTCG TCCATTACAT CTGGCTAACA AATTTGAAGA
  781 CAAAATTGTG GCATATACTG AAAAGAAAAT GACCAGTGGC AAAATCAAGA AGTTTATTCA
  841 GGAAAGCATT TTTTGGTCTC TGTCCTCATA TGACAGAAGA TAATAAAGAT TTGATACAAG
  901 GCAAGGACTT ACTCACGGCT TACTATGATG TGGACTATGA AAAGAATACT AAAGGTTCTA
  961 ACTACTGGGA GAAACAGGTT CATGGATGGG TGGCAAAGAC ATTCCCTTGG ATGCTGGACA
 1021 CGAACTCAAA CTTTGCTGTA GCTAGCCCGT AAAACCGTTT AGCCAATGAA TGGTCCTGAA
1081 CCTTCGACCT TAGGAAAGCA CTAACCTGGA AGAGAGAATC
//
Primer: mCherry-N_rev
Sequence file: RDB15153_A7Flb.seq check
>D04425A2_A7Fl_2_mCherry-N_R_F07_16_ABI24.ab1
    1 TCGCAGGAAC CTTCCTTGAT GATGGCCATG TTATCCTCCT CGCCCTTGCT CACCATGGTG
   61 GCGACCGGTG GATCCCGGGC CCGCGGGAGG TCCTCTTGTG CCTTCTTCTT CTTCTTAGGT
  121 TTTTCTTCTT GAATTATAGG AGGGTTTGTA GCTTCTCGTT GTAGATAGCT GATAAAATCA
  181 TTTAATTCAC GGCCACCTTC ATACTTCTTT GGAGTTAGCT TCTTGTTGGC TGGTGAGAAG
  241 TAGATGGTAG GAAAACCCTT GACTTCATAT GGAGAAGGCA CATCATTGGC TGTGGCATCC
  301 ATCTTGGCTA TGACAATATT TGGGTCTTTG CTGAGTTTCT CCCCCAGCTC TTTGTACTTG
  361 GGTTCCAGGT TCTTACAGTG GCCACACCAA GGAGCATAAA ACTCGATCAG CACGTCCTTG
  421 TCTTCAGCAT TCACTATGTC ATCAAAACTC TCTGCTACCA CCACCTTGAC AGGTCCTTCG
  481 TTGGTCTCGG GGATAGGTTC AGACTTCAGG TATCTCTTCA AGTTGCCATC AAAGTATTCC
  541 TGCAGGAACC GCTCAAGAGC CTTGCCATCC CGCGAGAACT CCTCCTGCAT GACAAACTTC
  601 TCTCCTTTAG CAGTTCTGAT AGCCACAACA GGAATCTCTC CAGTAGTGCT TTCTAAGCCA
  661 AAGTCAGACA ATTCATGGCT AAAGGTTTTA CGGCTAGCTA CAGCAAAGTT GAGTTTGTGT
  721 CCAGCATCAA GGAATGTCTT TGCCACCATC ATGACCCTGT TTCTCCAGTA GTTAGAACCT
  781 TTAGTATTCT TTTCATAGTC CACATCATAG TAAGCCGTGA GTAAGTCCTT GCCTTGTATC
  841 AAATCTTTAT TATCTTCTGT CATATGAGGA CAGAGACCAA AAAATGCTTT TCCTGAATAA
  901 ACTTCTTGAT TTTGCCACTG GTCATTTTTC TTTTCAGTAT ATGCCACAAT TTTTGTCTTC
  961 AAATTTGTTA GCCAGATGTA AATGGACGAA AATATAGTAA ATCCCCCTCT CCATTATCAT
 1021 CGTACTCCCT TCACAGAGGA TTCAAACGTT GGTTGTGTGC AAATCGGGTA GGTTTATTCT
1081 CTCAGTGCCT GGGCCTGGCC TTTTTAGGAA CTTCGGAAGT TGGCCAATA
//
Primer: SV40polyA-R
Sequence file: RDB15153_A7Flc.seq check
>D04425A2_A7Fl_2_SV40polyA-R_G07_19_ABI24.ab1
    1 AAAAATTGGG AGTTTTTAAG CAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC
   61 TAGAGTCGCG GCCGCTAGAG CTCATCCTTC TTGTACAGCT CGTCCATGCC GCCGGTGGAG
  121 TGGCGGCCCT CGGCGCGTTC GTACTGTTCC ACGATGGTGT AGTCCTCGTT GTGGGAGGTG
  181 ATGTCCAACT TGATGTTGAC GTTGTAGGCG CCGGGCAGCT GCACGGGCTT CTTGGCCTTG
  241 TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG CCTCTGCTTG
  301 ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC CTCCCAGCCC
  361 ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG CAGCTTCACC
  421 TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC CACGCCGCCG
  481 TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG CTTCAAGTAG
  541 TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACATGAA CTGAGGGGAC
  601 AGGATGTCCC AGGCGAAGGG CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG
  661 GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG GCCGTTCACG
  721 GAGCCCTCCA TGTGCACCTT GAAGCGCATG AACTCCTTGA TGATGGCCAT GTTATCCTCC
  781 TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCCGGG CCCGCGGGAG GTCCTCTTGT
  841 GCCTTCTTCT TCTTCTTAGG TTTTTCTTCT TGAATTATAG GAGGGTTTGT AGCTTCTCGT
  901 TGTAGATAGC TGATAAAATC ATTTTAATTC ACGGCCACCT TCATACTTCT TTGGAGTTAG
  961 CTTTCTTGTT TGGCTGGGTG AGAAGTAGAT GGTAGGAAAA ACCCTTGACT TCATATGGGA
 1021 GGAAGCACCA TCAATTGGGC TGTGGCAATC AATCTTGGCC TAATGACCAT ATTGGGGTCC
1081 TTTGGCCTGA AGTTTCTCCC TCAGCTCTTT GTACTTGGTA TTCCAGGCAA ATC
//
Primer: SV40pro_F_V2
Sequence file: RDB15153_A7Fld.seq check
>D04425A2_A7F1_2_SV40pro_F_V2_F06_18_ABI24.ab1
    1 AGGGGCGGGA GATTCGAGCT TTTTTGGAGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC
   61 AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC
  121 TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC
  181 CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC
  241 CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG
  301 CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT
  361 GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC
  421 CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA
  481 CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA
  541 TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT
  601 CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC
  661 GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT
  721 GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG
  781 CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT
  841 CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC
  901 GACCAAGCGA CGCCCAACCC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA
  961 AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGG
 1021 ATCTCATGCT GGAGTTCTTC GCCCACCCTA AGGGGGAGGC TAACTGAAAA CACGGGAAGG
 1081 AGAAACATAC CGGGAAGGAA ACCCGCGGCT ATGGAACGGC ATTAAAAGGA CGGAATTAAA
1141 AACCGCACGG GTGTTTGGGT TCGTTTTTTG TTTCAAATAA AAGACGGCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]