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PDIA3-mCherry-KDEL

Expression vector of rat Protein disulfide-isomerase A3 (PDIA3), mCherry-ER retention signal (KDEL) tag.

Catalog number RDB15153
Resource name PDIA3-mCherry-KDEL
Clone info. Expression vector of rat Protein disulfide-isomerase A3 (PDIA3) with C-terminally tagged mCherry-ER retention signal (KDEL). cDNA fragment 74nt to 1588nt of NM_017319.1 is cloned.
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy. Known differences (at least): C to T substitution at 1499nt of NM_017319.1 (Leu to Phe).
Vector backbone pmCherry-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Pdia3 cDNA
Depositor|Developer Hamachi, Itaru |
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External Database
rat Pdia3

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Methods 13 (11): 931-937, 2016)。
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          RDB15153 PDIA3-mCherry-KDEL DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The PDIA3-mCherry-KDEL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15153).

          Reference section:

          Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15153_A7Flp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB15153_A7Fla.seq check
          >D04425A2_A7Fl_2_CMV-Forward_E07_13_ABI24.ab1
              1 ACTTTCAAAC TCCCGCCCCC ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
             61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG ACTCAGATCT
            121 CGAGGCCACC ATGCGCTTCA GCTGCCTTGC GCTGCTCCCG GGCGTGGCGT TGCTGCTCGC
            181 CTCGGCCCTC CTCGCCTCCG CCTCAGACGT GTTGGAACTG ACGGACGAAA ACTTCGAGAG
            241 TCGCGTCTCC GACACGGGCT CAGCTGGGCT CATGCTAGTC GAGTTCTTCG CCCCATGGTG
            301 TGGACATTGC AAGAGGCTTG CCCCTGAGTA TGAAGCTGCA GCAACCAGAT TAAAAGGAAT
            361 AGTCCCATTA GCAAAGGTGG ACTGCACTGC CAACACAAAC ACCTGTAATA AGTATGGCGT
            421 CAGCGGCTAC CCAACTCTTA AAATATTTAG AGATGGTGAA GAAGCGGGTG CTTATGATGG
            481 GCCTAGGACT GCCGATGGAA TTGTCAGCCA CTTGAAGAAA CAAGCAGGAC CAGCTTCAGT
            541 TCCTCTCAGG ACTGAGGACG AATTTAAGAA GTTCATTAGT GATAAAGATG CCTCGGTGGT
            601 GGGCTTTTTC AGGGATTTAT TCAGTGATGG CCACTCCGAG TTCCTAAAAG CAGCCAGCAA
            661 CTTGAGAGAT AACTACCGAT TTGCACACAC CAACGTTGAA TCTCTGGTGA AGGAGTACGA
            721 TGATAATGGA GAGGGGATTA CTATATTTCG TCCATTACAT CTGGCTAACA AATTTGAAGA
            781 CAAAATTGTG GCATATACTG AAAAGAAAAT GACCAGTGGC AAAATCAAGA AGTTTATTCA
            841 GGAAAGCATT TTTTGGTCTC TGTCCTCATA TGACAGAAGA TAATAAAGAT TTGATACAAG
            901 GCAAGGACTT ACTCACGGCT TACTATGATG TGGACTATGA AAAGAATACT AAAGGTTCTA
            961 ACTACTGGGA GAAACAGGTT CATGGATGGG TGGCAAAGAC ATTCCCTTGG ATGCTGGACA
           1021 CGAACTCAAA CTTTGCTGTA GCTAGCCCGT AAAACCGTTT AGCCAATGAA TGGTCCTGAA
          1081 CCTTCGACCT TAGGAAAGCA CTAACCTGGA AGAGAGAATC
          //
          Primer: mCherry-N_rev
          Sequence file: RDB15153_A7Flb.seq check
          >D04425A2_A7Fl_2_mCherry-N_R_F07_16_ABI24.ab1
              1 TCGCAGGAAC CTTCCTTGAT GATGGCCATG TTATCCTCCT CGCCCTTGCT CACCATGGTG
             61 GCGACCGGTG GATCCCGGGC CCGCGGGAGG TCCTCTTGTG CCTTCTTCTT CTTCTTAGGT
            121 TTTTCTTCTT GAATTATAGG AGGGTTTGTA GCTTCTCGTT GTAGATAGCT GATAAAATCA
            181 TTTAATTCAC GGCCACCTTC ATACTTCTTT GGAGTTAGCT TCTTGTTGGC TGGTGAGAAG
            241 TAGATGGTAG GAAAACCCTT GACTTCATAT GGAGAAGGCA CATCATTGGC TGTGGCATCC
            301 ATCTTGGCTA TGACAATATT TGGGTCTTTG CTGAGTTTCT CCCCCAGCTC TTTGTACTTG
            361 GGTTCCAGGT TCTTACAGTG GCCACACCAA GGAGCATAAA ACTCGATCAG CACGTCCTTG
            421 TCTTCAGCAT TCACTATGTC ATCAAAACTC TCTGCTACCA CCACCTTGAC AGGTCCTTCG
            481 TTGGTCTCGG GGATAGGTTC AGACTTCAGG TATCTCTTCA AGTTGCCATC AAAGTATTCC
            541 TGCAGGAACC GCTCAAGAGC CTTGCCATCC CGCGAGAACT CCTCCTGCAT GACAAACTTC
            601 TCTCCTTTAG CAGTTCTGAT AGCCACAACA GGAATCTCTC CAGTAGTGCT TTCTAAGCCA
            661 AAGTCAGACA ATTCATGGCT AAAGGTTTTA CGGCTAGCTA CAGCAAAGTT GAGTTTGTGT
            721 CCAGCATCAA GGAATGTCTT TGCCACCATC ATGACCCTGT TTCTCCAGTA GTTAGAACCT
            781 TTAGTATTCT TTTCATAGTC CACATCATAG TAAGCCGTGA GTAAGTCCTT GCCTTGTATC
            841 AAATCTTTAT TATCTTCTGT CATATGAGGA CAGAGACCAA AAAATGCTTT TCCTGAATAA
            901 ACTTCTTGAT TTTGCCACTG GTCATTTTTC TTTTCAGTAT ATGCCACAAT TTTTGTCTTC
            961 AAATTTGTTA GCCAGATGTA AATGGACGAA AATATAGTAA ATCCCCCTCT CCATTATCAT
           1021 CGTACTCCCT TCACAGAGGA TTCAAACGTT GGTTGTGTGC AAATCGGGTA GGTTTATTCT
          1081 CTCAGTGCCT GGGCCTGGCC TTTTTAGGAA CTTCGGAAGT TGGCCAATA
          //
          Primer: SV40polyA-R
          Sequence file: RDB15153_A7Flc.seq check
          >D04425A2_A7Fl_2_SV40polyA-R_G07_19_ABI24.ab1
              1 AAAAATTGGG AGTTTTTAAG CAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC
             61 TAGAGTCGCG GCCGCTAGAG CTCATCCTTC TTGTACAGCT CGTCCATGCC GCCGGTGGAG
            121 TGGCGGCCCT CGGCGCGTTC GTACTGTTCC ACGATGGTGT AGTCCTCGTT GTGGGAGGTG
            181 ATGTCCAACT TGATGTTGAC GTTGTAGGCG CCGGGCAGCT GCACGGGCTT CTTGGCCTTG
            241 TAGGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG CCTCTGCTTG
            301 ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGGAGGC CTCCCAGCCC
            361 ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG CAGCTTCACC
            421 TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC CACGCCGCCG
            481 TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG CTTCAAGTAG
            541 TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACATGAA CTGAGGGGAC
            601 AGGATGTCCC AGGCGAAGGG CAGGGGGCCA CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG
            661 GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG GCCGTTCACG
            721 GAGCCCTCCA TGTGCACCTT GAAGCGCATG AACTCCTTGA TGATGGCCAT GTTATCCTCC
            781 TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCCGGG CCCGCGGGAG GTCCTCTTGT
            841 GCCTTCTTCT TCTTCTTAGG TTTTTCTTCT TGAATTATAG GAGGGTTTGT AGCTTCTCGT
            901 TGTAGATAGC TGATAAAATC ATTTTAATTC ACGGCCACCT TCATACTTCT TTGGAGTTAG
            961 CTTTCTTGTT TGGCTGGGTG AGAAGTAGAT GGTAGGAAAA ACCCTTGACT TCATATGGGA
           1021 GGAAGCACCA TCAATTGGGC TGTGGCAATC AATCTTGGCC TAATGACCAT ATTGGGGTCC
          1081 TTTGGCCTGA AGTTTCTCCC TCAGCTCTTT GTACTTGGTA TTCCAGGCAA ATC
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB15153_A7Fld.seq check
          >D04425A2_A7F1_2_SV40pro_F_V2_F06_18_ABI24.ab1
              1 AGGGGCGGGA GATTCGAGCT TTTTTGGAGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC
             61 AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC
            121 TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC
            181 CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC
            241 CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG
            301 CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT
            361 GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC
            421 CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA
            481 CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA
            541 TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT
            601 CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC
            661 GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT
            721 GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG
            781 CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT
            841 CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC
            901 GACCAAGCGA CGCCCAACCC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA
            961 AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGG
           1021 ATCTCATGCT GGAGTTCTTC GCCCACCCTA AGGGGGAGGC TAACTGAAAA CACGGGAAGG
           1081 AGAAACATAC CGGGAAGGAA ACCCGCGGCT ATGGAACGGC ATTAAAAGGA CGGAATTAAA
          1141 AACCGCACGG GTGTTTGGGT TCGTTTTTTG TTTCAAATAA AAGACGGCG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

          2023.12.13

          GNP_filter3_RDBDEP_html_231213.pl