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pCX-PURO-masFSH

Expression vector of the glycoprotein hormones alpha chain (alpha GSU) and follicle-stimulating hormone beta subunit (FSH beta) of mastmys, simultaneously.

Catalog number RDB15151
Resource name pCX-PURO-masFSH
Alternative name pCX-PURO-Mastomys-FSH
Clone info. Expression vector of the glycoprotein hormones alpha chain (alpha GSU) and follicle-stimulating hormone beta subunit (FSH beta) of mastomys, simultaneously by the dual CAG promoter, see Fig1 and Fig S6 of Ohta, A. J. Reprod. Dev. 2017. Please refer AF307149 for mastmoyst alpha GSU and AY458603 for mastmoyst FSH beta.
Expression was confirmed by the depositor with immunocytochemistry. This study was designed and performed by late Dr. Akihiko Ota.
Vector backbone pCX-puro (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mastmoys alpha GSU cDNA mastmoys FSH beta cDNA
Depositor Kato, Yukio |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ohta, A. J. Reprod. Dev. 2017) and an acknowledgment to the DEPOSITOR are requested.
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RDB15151 pCX-PURO-masFSH Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Ohta, A., Construction and expression of vectors encoding biologically active rodent gonadotropins. J. Reprod. Dev. 63 (6): 605-609 (2017). PMID 29033405.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Ohta, A., Construction and expression of vectors encoding biologically active rodent gonadotropins. J. Reprod. Dev. 63 (6): 605-609 (2017). PMID 29033405.

2019.07.17

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