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pCX-PURO-ｍasFSH

Expression vector of the glycoprotein hormones alpha chain (alpha GSU) and follicle-stimulating hormone beta subunit (FSH beta) of mastmys, simultaneously.

Catalog number RDB15151
Resource name pCX-PURO-ｍasFSH
Alternative name pCX-PURO-Mastomys-FSH
Clone info. Expression vector of the glycoprotein hormones alpha chain (alpha GSU) and follicle-stimulating hormone beta subunit (FSH beta) of mastomys, simultaneously by the dual CAG promoter, see Fig1 and Fig S6 of Ohta, A. J. Reprod. Dev. 2017. Please refer AF307149 for mastmoyst alpha GSU and AY458603 for mastmoyst FSH beta.
Comment Expression was confirmed by the depositor with immunocytochemistry. This study was designed and performed by late Dr. Akihiko Ota.
Vector backbone pCX-puro (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mastmoys alpha GSU cDNA mastmoys FSH beta cDNA
Depositor|Developer Kato, Yukio |
Other clones in our bank
External Database
mastmoys alpha GSU mastmoys FSH beta
        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ohta, A. J. Reprod. Dev. 2017) and an acknowledgment to the DEPOSITOR are requested.
        Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
        Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって開発者の指定する文献 （Ohta, A. J. Reprod. Dev. 2017) を引用し、寄託者への謝辞の表明を必要とする。
        付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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        RDB15151 pCX-PURO-ｍasFSH Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Electronic file

        Original reference

        original Ohta, A., Construction and expression of vectors encoding biologically active rodent gonadotropins. J. Reprod. Dev. 63 (6): 605-609 (2017). PMID 29033405.

        Further references such as user reports and related articles (go to bottom)

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        Sequence information

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References

        Original, user report and related articles

        original Ohta, A., Construction and expression of vectors encoding biologically active rodent gonadotropins. J. Reprod. Dev. 63 (6): 605-609 (2017). PMID 29033405.

        2022.05.17

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