Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCX-PURO-ｍouFSH

Expression vector of the glycoprotein hormones alpha chain (alpha GSU) and follicle-stimulating hormone beta subunit (FSH beta) of mouse, simultaneously.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ohta, A. J. Reprod. Dev. 2017) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

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Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

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提供条件	利用者は、研究成果の公表にあたって開発者の指定する文献 （Ohta, A. J. Reprod. Dev. 2017) を引用し、寄託者への謝辞の表明を必要とする。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15149_A7Fgp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCga_R1 (Pr1308) Region: insert mid(mCga 5',CAG pro.1) Sequence file: RDB15149_A7Fga.seq
>D04674A4_A7Fg_1_mCga_R1_A03_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTTTGNN NNCNCAGCAT GTGGCCTCCG AGGTAATATT    61 CTTTGGAACC NGCATTGTCT TCTTGGACCT GGCGGGAGTG GGATATGCCC TGGAGAAGCA   121 ACAGCCCATA CACTGGTAGA TGGGGGCTCC TAGCTTGGAG AAGTATTTAT TTTCCTTTAG   181 TTTACATTCT GGGCAACCCT GAATAATAAA GTCTCCATCA GGAAGAGAAT GAAGAATATG   241 CAGGAACATG GACAGCATGA CCAGAATGAC AGCTGCATAT TTTCTGTAGT AATCCATAGC   301 TCTTCTGCAC GCGTGACAAT CGAATTCTTT GCCAAAATGA TGAGACAGCA CAATAACCAG   361 CACGTTGCCC AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTCT   421 AGAGCCGCCG GTCACACGCC AGAAGCCGAA CCCCGCCCTG CCCCGTCCCC CCCGAAGGCA   481 GCCGTCCCCC CGCGGACAGC CCCGAGGCTG GAGAGGGAGA AGGGGACGGC GGCGCGGCGA   541 CGCACGAAGG CCCTCCCCGC CCATTTCCTT CCTGCCGGCG CCGCACCGCT TCGCCCCGCG   601 CCCGCTAGAG GGGGTGCGGC GGCGCCTCCC AGATTTCGGC TCCGCACAGA TTTGGGACAA   661 AGGAAGTCCC TGCGCCCTCT CGCACGATTA CCATAAAAGG CAATGGCTGC GGCTCGCCGC   721 GCCTCGACAG CCGCCGGCGC TCCGGGGGCC GCCGCGCCCC TCCCCCGAGC CCTCCCCGGC   781 CCGNGGCGGC CCCGCCCCGC CCGGCACCCC CACCTGCCGC CACCCCCCCG CCCGGCACGG   841 CGAGCCCCGC GCNACGCCCC GTACNGAGCC CCCGCACCCN ANNCCNGGNC NNGCTCANCN 901 ACNCNGGGNN GGGGNGNNNN NNN //
Primer: mCga_F1 (Pr1307) Region: insert mid(mCga 3',beta-globin polyA.1) Sequence file: RDB15149_A7Fgb.seq
>D04674A4_A7Fg_1_mCag_F1_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NTTNNTGNNT ATTCTTNNTT CTCTTCCTGA TGGAGACTTT    61 ATTATTCAGG GNNGNNNNNA ATGTAAACTA AAGGAAAATA AATACTTCTC CAAGCTAGGA   121 GCCCCCATCT ACCAGTGTAT GGGCTGTTGC TTCTCCAGGG CATATCCCAC TCCCGCCAGG   181 TCCAAGAAGA CAATGCTGGT TCCAAAGAAT ATTACCTCGG AGGCCACATG CTGTGTGGCC   241 AAAGCATTTA CTAAGGCCAC AGTAATGGGA AATGCCAGAG TGGAGAATCA TACGGAGTGC   301 CACTGTAGCA CTTGCTACTA CCACAAGTCG TAGCTTCTGA GACGCGTAGC AATCACTAGT   361 GAATTCACTC CTCAGGTGCA GGCTGCCTAT CAGAAGGTGG TGGCTGGTGT GGCCAATGCC   421 CTGGCTCACA AATACCACTG AGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG   481 AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG   541 TGTTGGAATT TTTTGTGTCT CTCACTCGGA AGGACATATG GGAGGGCAAA TCATTTAAAA   601 CATCAGAATG AGTATTTGGT TTAGAGTTTG GCAACATATG CCCATATGCT GGCTGCCATG   661 AACAAAGGTT GGCTATAAAG AGGTCATCAG TATATGAAAC AGCCCCCTGC TGTCCATTCC   721 TTATTCCATA GAAAAGCCTT GACTTGANGT TAGANTTTTT TTATATTTTG TTTTGTGTTA   781 TTTTTTTCTT TAACATCCCT AAAATTTTCC TTACATGTTT TACTANCNNG ANTTTTCCTC   841 CTCTCCTGAN TACTNCNAGN CATAGCTGTC CCTCTTCTCN NATNGANATC CCTNNNNCNG   901 CANNCNNNGC TGANATTNNN TATTGACTNN TNATTANNAN TNATCNNNTN NNGGGNCNNN 961 ANNTNCNNAN NNNNNATNNG GNNNTNNNNN NNNNN //
Primer: pCAG-F (Pr0328) Region: insert mid(mFsh b 5') Sequence file: RDB15149_A7Fgc.seq
>15149_15149_A7Fg_1P_pCAG-F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGCANGG CGGGGTTCNN NNNNCTGGCG    61 TGTGACCGGC GNNNNNNNNN CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA   121 GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCGATTG   181 CTGCGGCCGC GGGAATTCGA TTCAGTCTAG ACCAGACCAT GATGAAGTTG ATCCAGCTTT   241 GCATCTTATT CTGGTGCTGG AGAGCAATCT GCTGCCATAG CTGTGAANTG ACCAACATCA   301 CCATCTCAGT AGAGAAGGAA GAGTGCCGTT TCTGCATAAN CATCAATACC ACTTGGTGTG   361 CGGGCTACTG CTACACTAGG GATCTGNNNN NTAAGGACCC NNNTCGGNCC AATACCCANA   421 AAGNNTGNAC CNTCNANGAG CTGGTATACN ANNNNNNNAN NNNGNCTGGN TGTGNCCGNC 481 NCTCANANTC CCTCNACNNN NNNNCNNNNN NNNNN //
Primer: M13R (Pr0160) Region: insert mid(beta-globin polyA.2,mFsh b 3') Sequence file: RDB15149_A7Fgd.seq
>D04674A4_A7Fg_1_M13Rv_H03_08_ABI08.ab1     1 NNNNNNNNNG NNNNNNNNGN NNNNNNNNNC CNTAAGAGAA GANGGNNAGC TATGACTGGG    61 NNTAGTCAGG AGAGGAGGAA AAATCTGGCT AGTAAAACAT GTAAGGAAAA TTTTAGGGAT   121 GTTAAAGAAA AAAATAACAC AAAACAAAAT ATAAAAAAAA TCTAACCTCA AGTCAAGGCT   181 TTTCTATGGA ATAAGGAATG GACAGCAGGG GGCTGTTTCA TATACTGATG ACCTCTTTAT   241 AGCCAACCTT TGTTCATGGC AGCCAGCATA TGGGCATATG TTGCCAAACT CTAAACCAAA   301 TACTCATTCT GATGTTTTAA ATGATTTGCC CTCCCATATG TCCTTCCGAG TGAGAGACAC   361 AAAAAATTCC AACACACTAT TGCAATGAAA ATAAATTTCC TTTATTAGCC AGAAGTCAGA   421 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCTCAGTGG   481 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC   541 TGAGGAGTGA ATTCACTAGT GATTGCTGCG GCCGCGAATT CACTAGTGAT TCGATCTAGA   601 GTCCATCGTC GTTTATTCCT TCATTTCACT GAAGGAGCAG TAGCTGGGTC CTAGGCCCCT   661 CACGGTGCAG TCAGTGCTGT CGCTGTCACA CTTGCCACAG TGACATTCAG TGGCTACTGG   721 ATATGTGTAG AGGGAGTCTG AGTGGCGGGC ACAGCCAGGC AATCTTACGG TCTCGTATAC   781 CAGCTCCTTG AANGTACATA CTTTCTGGGT ATTGGGCCGA NCTGGGNCCT TATACACCAG   841 ATNCCNAGTG TAGCANTANC CCGCACACCN NNNGGTATTG ATGCTTATGC ANAAACGGCA   901 NTCNNNCTTC TCTANTGANA TGGNGANGNT GGNCANTTCA NAGCTANNNN ANCANANNGN 961 TCNNCNNNNN NNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro,insert 5'(CMV en.1,CAG pro.1) Sequence file: RDB15149_A7Fge.seq
>D04674A4_A7Fg_1_Amp_R_G02_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TATNAGGGTT ATTGTCTCNT GAGCGGANAC    61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA   121 GTGCCACCTG GGTCGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC   181 ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC   241 TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT   301 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA   361 CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG   421 TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA   481 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCGAG GTGAGCCCCA CGTTCTGCTT   541 CACTCTCCCC ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT   601 ATTTTGTGCA GCGATGGGGG CGGGGGGGGG GGGGGNNNNN NNNCCAGGCG GNNNGGGNCG   661 NNNNNNGGGG CGGGGCGGNN CNAGNNNAAN AGNNNCGGCG NNNNCNNNNN NAANNGNNNN 721 NNNNNN //
Primer: SV40pA3element Reverse (Pr0108) Region: SV40 pro_ori,PuroR Sequence file: RDB15149_A7Fgf.seq
>D04674A4_A7Fg_1_SV40pA3'element Reverse_A02_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCNNGCAA CGCGGCCTTT TTACGGTTCC    61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG   121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC   181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG   241 CGCGTTGGCC GATTCATTAA TGCAGCGGAT CCTGTGGAAT GTGTGTCAGT TAGGGTGTGG   301 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC   361 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT   421 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC   481 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA   541 GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG   601 CTTTTGCAAA AAGCTTGCAT GCCTGCAGGT CGGCCGCCAC GACCGGTGCC GCCACCATCC   661 CCTGACCCAC GCCCCTGACC CCTCACAAGG AGACGACCTT CCATGACCGA NTACAAGCCC   721 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG   781 TTCGCCGANT ACCCCGCCAC GCGCCACACC GTCNACCCGG ACCGCCACAT CGAGCGGGNC   841 ANCGANCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGNGNGGGTC   901 GCGNANGANG GNGNCNNNGN GGCGGNNTGG ACCACGCNNG ANNNCGNCGA ANNGGGGGGN 961 NGNGNTNCNN NNANNNCGNN NNNNNNNN //
Primer: PuroR_F (Pr0554) Region: SV40 pA Sequence file: RDB15149_A7Fgg.seq
>D04674A4_A7Fg_1_PuroR_F_F02_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN NNGNNCGCGC ACCTNNNNNN NTGACCCGCA    61 AGCCCGGTGC CTGNCGCCCG CCCCACGACC CGCAGCGCCC GACCGAAAGG AGCGCACGAC   121 CCCATGGCTC CGACCGAAGC CACCCGGGGC GGCCCCGCCG ACCCCGCACC CGCCCCCGAG   181 GCCCACCGAC TCTAGAGGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT   241 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT   301 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC   361 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA   421 TCTTATCATG TCTGGATCGA TCCGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG   481 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT   541 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA   601 CAGCTATGAC CATGATTACG CCAAGCTTGG GCTGCAGGTC GAGGGATCTC CATAAGAGAA   661 GAGGGACAGC TATGACTGGG AGTAGTCAGG AGAGGAGGAA AAATCTGGCT AGTAAAACAT   721 GTAAGGAAAA TTTTAGGGAT GTTAAAGAAA AAANTAACAC AAAACAAAAT ATAAAAAAAA   781 TCTAACCTCA AGTCAAGGCT TTTCTATGGA ATNANGAATG GACAGCANGG GGCTGTTNCN   841 TATACTGATG ACCTCTTTAT AGCCNACCTT TGTNCATGGC AGCCAGCATA TGGGCATATG   901 TTGCCNAANT CTAAANCNAA ANANNCNTTC TGANGNTTNN AANGANTTGC CNNNCCNNAN 961 GNNNTTNCNN ANNGANANAN NNNAAAANNN NNNANNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.07.27

GNP_filter3_RDBDEP_html_230702.pl