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pCX-PURO-ｍouFSH (#RDB15149)

Expression vector of the glycoprotein hormones alpha chain (alpha GSU) and follicle-stimulating hormone beta subunit (FSH beta) of mouse, simultaneously.

Alternative name pCX-PURO-Mouse-FSH
Clone info. Expression vector of the glycoprotein hormones alpha chain (alpha GSU) and follicle-stimulating hormone beta subunit (FSH beta) of mouse, simultaneously by the dual CAG promoter, see Fig1 and Fig S6 of Ohta, A. J. Reprod. Dev. 2017.
Comment Expression was confirmed by the depositor with immunocytochemistry. This study was designed and performed by late Dr. Akihiko Ota.
Vector backbone pCX-puro (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Cga cDNA mouse Fshb cDNA
Depositor|Developer Kato, Yukio |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ohta, A. J. Reprod. Dev. 2017) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって開発者の指定する文献 （Ohta, A. J. Reprod. Dev. 2017) を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15149 pCX-PURO-ｍouFSH DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCX-PURO-ｍouFSH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15149).

Reference section:

Ohta, A., Tsunoda, Y., Tamura, Y., Iino, K., Nishimura, N., Nishihara, H., Takanashi, H., Yoshida, S., Kato, T., Kato, Y., Construction and expression of vectors encoding biologically active rodent gonadotropins. J. Reprod. Dev. 63 (6): 605-609 (2017). PMID 29033405. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15149_A7Fgp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCga_R1 (Pr1308)
Region: insert mid(mCga 5',CAG pro.1)
Sequence file: RDB15149_A7Fga.seq check
>D04674A4_A7Fg_1_mCga_R1_A03_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTTTGNN NNCNCAGCAT GTGGCCTCCG AGGTAATATT
   61 CTTTGGAACC NGCATTGTCT TCTTGGACCT GGCGGGAGTG GGATATGCCC TGGAGAAGCA
  121 ACAGCCCATA CACTGGTAGA TGGGGGCTCC TAGCTTGGAG AAGTATTTAT TTTCCTTTAG
  181 TTTACATTCT GGGCAACCCT GAATAATAAA GTCTCCATCA GGAAGAGAAT GAAGAATATG
  241 CAGGAACATG GACAGCATGA CCAGAATGAC AGCTGCATAT TTTCTGTAGT AATCCATAGC
  301 TCTTCTGCAC GCGTGACAAT CGAATTCTTT GCCAAAATGA TGAGACAGCA CAATAACCAG
  361 CACGTTGCCC AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTCT
  421 AGAGCCGCCG GTCACACGCC AGAAGCCGAA CCCCGCCCTG CCCCGTCCCC CCCGAAGGCA
  481 GCCGTCCCCC CGCGGACAGC CCCGAGGCTG GAGAGGGAGA AGGGGACGGC GGCGCGGCGA
  541 CGCACGAAGG CCCTCCCCGC CCATTTCCTT CCTGCCGGCG CCGCACCGCT TCGCCCCGCG
  601 CCCGCTAGAG GGGGTGCGGC GGCGCCTCCC AGATTTCGGC TCCGCACAGA TTTGGGACAA
  661 AGGAAGTCCC TGCGCCCTCT CGCACGATTA CCATAAAAGG CAATGGCTGC GGCTCGCCGC
  721 GCCTCGACAG CCGCCGGCGC TCCGGGGGCC GCCGCGCCCC TCCCCCGAGC CCTCCCCGGC
  781 CCGNGGCGGC CCCGCCCCGC CCGGCACCCC CACCTGCCGC CACCCCCCCG CCCGGCACGG
  841 CGAGCCCCGC GCNACGCCCC GTACNGAGCC CCCGCACCCN ANNCCNGGNC NNGCTCANCN
901 ACNCNGGGNN GGGGNGNNNN NNN
//
Primer: mCga_F1 (Pr1307)
Region: insert mid(mCga 3',beta-globin polyA.1)
Sequence file: RDB15149_A7Fgb.seq check
>D04674A4_A7Fg_1_mCag_F1_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTTNNTGNNT ATTCTTNNTT CTCTTCCTGA TGGAGACTTT
   61 ATTATTCAGG GNNGNNNNNA ATGTAAACTA AAGGAAAATA AATACTTCTC CAAGCTAGGA
  121 GCCCCCATCT ACCAGTGTAT GGGCTGTTGC TTCTCCAGGG CATATCCCAC TCCCGCCAGG
  181 TCCAAGAAGA CAATGCTGGT TCCAAAGAAT ATTACCTCGG AGGCCACATG CTGTGTGGCC
  241 AAAGCATTTA CTAAGGCCAC AGTAATGGGA AATGCCAGAG TGGAGAATCA TACGGAGTGC
  301 CACTGTAGCA CTTGCTACTA CCACAAGTCG TAGCTTCTGA GACGCGTAGC AATCACTAGT
  361 GAATTCACTC CTCAGGTGCA GGCTGCCTAT CAGAAGGTGG TGGCTGGTGT GGCCAATGCC
  421 CTGGCTCACA AATACCACTG AGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG
  481 AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG
  541 TGTTGGAATT TTTTGTGTCT CTCACTCGGA AGGACATATG GGAGGGCAAA TCATTTAAAA
  601 CATCAGAATG AGTATTTGGT TTAGAGTTTG GCAACATATG CCCATATGCT GGCTGCCATG
  661 AACAAAGGTT GGCTATAAAG AGGTCATCAG TATATGAAAC AGCCCCCTGC TGTCCATTCC
  721 TTATTCCATA GAAAAGCCTT GACTTGANGT TAGANTTTTT TTATATTTTG TTTTGTGTTA
  781 TTTTTTTCTT TAACATCCCT AAAATTTTCC TTACATGTTT TACTANCNNG ANTTTTCCTC
  841 CTCTCCTGAN TACTNCNAGN CATAGCTGTC CCTCTTCTCN NATNGANATC CCTNNNNCNG
  901 CANNCNNNGC TGANATTNNN TATTGACTNN TNATTANNAN TNATCNNNTN NNGGGNCNNN
961 ANNTNCNNAN NNNNNATNNG GNNNTNNNNN NNNNN
//
Primer: pCAG-F (Pr0328)
Region: insert mid(mFsh b 5')
Sequence file: RDB15149_A7Fgc.seq check
>15149_15149_A7Fg_1P_pCAG-F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGCANGG CGGGGTTCNN NNNNCTGGCG
   61 TGTGACCGGC GNNNNNNNNN CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA
  121 GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTCGATTG
  181 CTGCGGCCGC GGGAATTCGA TTCAGTCTAG ACCAGACCAT GATGAAGTTG ATCCAGCTTT
  241 GCATCTTATT CTGGTGCTGG AGAGCAATCT GCTGCCATAG CTGTGAANTG ACCAACATCA
  301 CCATCTCAGT AGAGAAGGAA GAGTGCCGTT TCTGCATAAN CATCAATACC ACTTGGTGTG
  361 CGGGCTACTG CTACACTAGG GATCTGNNNN NTAAGGACCC NNNTCGGNCC AATACCCANA
  421 AAGNNTGNAC CNTCNANGAG CTGGTATACN ANNNNNNNAN NNNGNCTGGN TGTGNCCGNC
481 NCTCANANTC CCTCNACNNN NNNNCNNNNN NNNNN
//
Primer: M13R (Pr0160)
Region: insert mid(beta-globin polyA.2,mFsh b 3')
Sequence file: RDB15149_A7Fgd.seq check
>D04674A4_A7Fg_1_M13Rv_H03_08_ABI08.ab1
    1 NNNNNNNNNG NNNNNNNNGN NNNNNNNNNC CNTAAGAGAA GANGGNNAGC TATGACTGGG
   61 NNTAGTCAGG AGAGGAGGAA AAATCTGGCT AGTAAAACAT GTAAGGAAAA TTTTAGGGAT
  121 GTTAAAGAAA AAAATAACAC AAAACAAAAT ATAAAAAAAA TCTAACCTCA AGTCAAGGCT
  181 TTTCTATGGA ATAAGGAATG GACAGCAGGG GGCTGTTTCA TATACTGATG ACCTCTTTAT
  241 AGCCAACCTT TGTTCATGGC AGCCAGCATA TGGGCATATG TTGCCAAACT CTAAACCAAA
  301 TACTCATTCT GATGTTTTAA ATGATTTGCC CTCCCATATG TCCTTCCGAG TGAGAGACAC
  361 AAAAAATTCC AACACACTAT TGCAATGAAA ATAAATTTCC TTTATTAGCC AGAAGTCAGA
  421 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCTCAGTGG
  481 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC
  541 TGAGGAGTGA ATTCACTAGT GATTGCTGCG GCCGCGAATT CACTAGTGAT TCGATCTAGA
  601 GTCCATCGTC GTTTATTCCT TCATTTCACT GAAGGAGCAG TAGCTGGGTC CTAGGCCCCT
  661 CACGGTGCAG TCAGTGCTGT CGCTGTCACA CTTGCCACAG TGACATTCAG TGGCTACTGG
  721 ATATGTGTAG AGGGAGTCTG AGTGGCGGGC ACAGCCAGGC AATCTTACGG TCTCGTATAC
  781 CAGCTCCTTG AANGTACATA CTTTCTGGGT ATTGGGCCGA NCTGGGNCCT TATACACCAG
  841 ATNCCNAGTG TAGCANTANC CCGCACACCN NNNGGTATTG ATGCTTATGC ANAAACGGCA
  901 NTCNNNCTTC TCTANTGANA TGGNGANGNT GGNCANTTCA NAGCTANNNN ANCANANNGN
961 TCNNCNNNNN NNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,insert 5'(CMV en.1,CAG pro.1)
Sequence file: RDB15149_A7Fge.seq check
>D04674A4_A7Fg_1_Amp_R_G02_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TATNAGGGTT ATTGTCTCNT GAGCGGANAC
   61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
  121 GTGCCACCTG GGTCGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC
  181 ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC
  241 TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT
  301 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA
  361 CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG
  421 TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA
  481 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCGAG GTGAGCCCCA CGTTCTGCTT
  541 CACTCTCCCC ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT
  601 ATTTTGTGCA GCGATGGGGG CGGGGGGGGG GGGGGNNNNN NNNCCAGGCG GNNNGGGNCG
  661 NNNNNNGGGG CGGGGCGGNN CNAGNNNAAN AGNNNCGGCG NNNNCNNNNN NAANNGNNNN
721 NNNNNN
//
Primer: SV40pA3element Reverse (Pr0108)
Region: SV40 pro_ori,PuroR
Sequence file: RDB15149_A7Fgf.seq check
>D04674A4_A7Fg_1_SV40pA3'element Reverse_A02_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCNNGCAA CGCGGCCTTT TTACGGTTCC
   61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
  121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC
  181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG
  241 CGCGTTGGCC GATTCATTAA TGCAGCGGAT CCTGTGGAAT GTGTGTCAGT TAGGGTGTGG
  301 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC
  361 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT
  421 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC
  481 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA
  541 GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG
  601 CTTTTGCAAA AAGCTTGCAT GCCTGCAGGT CGGCCGCCAC GACCGGTGCC GCCACCATCC
  661 CCTGACCCAC GCCCCTGACC CCTCACAAGG AGACGACCTT CCATGACCGA NTACAAGCCC
  721 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG
  781 TTCGCCGANT ACCCCGCCAC GCGCCACACC GTCNACCCGG ACCGCCACAT CGAGCGGGNC
  841 ANCGANCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGNGNGGGTC
  901 GCGNANGANG GNGNCNNNGN GGCGGNNTGG ACCACGCNNG ANNNCGNCGA ANNGGGGGGN
961 NGNGNTNCNN NNANNNCGNN NNNNNNNN
//
Primer: PuroR_F (Pr0554)
Region: SV40 pA
Sequence file: RDB15149_A7Fgg.seq check
>D04674A4_A7Fg_1_PuroR_F_F02_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN NNGNNCGCGC ACCTNNNNNN NTGACCCGCA
   61 AGCCCGGTGC CTGNCGCCCG CCCCACGACC CGCAGCGCCC GACCGAAAGG AGCGCACGAC
  121 CCCATGGCTC CGACCGAAGC CACCCGGGGC GGCCCCGCCG ACCCCGCACC CGCCCCCGAG
  181 GCCCACCGAC TCTAGAGGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT
  241 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT
  301 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
  361 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
  421 TCTTATCATG TCTGGATCGA TCCGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG
  481 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT
  541 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA
  601 CAGCTATGAC CATGATTACG CCAAGCTTGG GCTGCAGGTC GAGGGATCTC CATAAGAGAA
  661 GAGGGACAGC TATGACTGGG AGTAGTCAGG AGAGGAGGAA AAATCTGGCT AGTAAAACAT
  721 GTAAGGAAAA TTTTAGGGAT GTTAAAGAAA AAANTAACAC AAAACAAAAT ATAAAAAAAA
  781 TCTAACCTCA AGTCAAGGCT TTTCTATGGA ATNANGAATG GACAGCANGG GGCTGTTNCN
  841 TATACTGATG ACCTCTTTAT AGCCNACCTT TGTNCATGGC AGCCAGCATA TGGGCATATG
  901 TTGCCNAANT CTAAANCNAA ANANNCNTTC TGANGNTTNN AANGANTTGC CNNNCCNNAN
961 GNNNTTNCNN ANNGANANAN NNNAAAANNN NNNANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ohta, A., Construction and expression of vectors encoding biologically active rodent gonadotropins. J. Reprod. Dev. 63 (6): 605-609 (2017). PMID 29033405. [link to RRC of NBRP]