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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2-SYN-GFP-BGHpA

AAV2 expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)).

Catalog number RDB15113
Resource name pAAV2-SYN-GFP-BGHpA
Clone info. AAV2 expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)), bovine growth hormone polyadenylation signal (BGHpA), see Fig. 1A of Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017.
Expression was confrimed by the depositor with microscopic observation.
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Gene/insert name Human SYN1 genomic DNA
Depositor|Developer Hioki, Hiroyuki |
 
Other clones in our bank human SYN1 (NCBI Gene 6853) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infectious virus.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15113 pAAV2-SYN-GFP-BGHpA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15113_A7Fdp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N
Region: EGFP 5', SYN pro, AAV2 ITR(L)
Sequence file: RDB15113_A7Fda.seq check
>D04569A7-2_A7Fd_EGFP-N_G01_07_ABI08.ab1
    1 GACCCGGGGT AAGCTCCTCG CCCTTGCTCA CCATGGTGGC TCGAATCAAG CTTATCGATA
   61 CCGTCCGCCG CAGCGCAGAT GGTCGCGCCC GTGCCCCCCT ATCTCGCGCC TCGCGTGGTG
  121 CGGTCCGGCT GGGCCGGCGG CGGCGCGGAC GCGACCAAGG TGGCCGGGAA GGGGAGTTTG
  181 CGGGGGACCG GCGAGTGACG TCAGCGCGCC TTCAGTGCTG AGGCGGCGGT GGCGCGCGCC
  241 GCCAGGCGGG GGCGAAGGCA CTGTCCGCGG TGCTGAAGCT GGCAGTGCGC ACGCGCCTCG
  301 CCGCATCCTG TTTCCCCTCC CCCTCTCTGA TAGGGGATGC GCAATTTGGG GAATGGGGGT
  361 TGGGTGCTTG TCCAGTGGGT CGGGGTCGGT CGTCAGGTAG GCACCCCCAC CCCGCCTCAT
  421 CCTGGTCCTA AAACCCACTT GCACTCATAC GCAGGGCCCT CTGCAGGACC TCGAGATATC
  481 TAGACCCAGC TTTCTTGTAC AAAGTGGTAC GCGTGCGGCC GCAGGAACCC CTAGTGATGG
541 AGTTGGCCAC TCCCTCTCTG CGCGCTC
//
Primer: EGFP-C-rev
Region: EGFP
Sequence file: RDB15113_A7Fdb.seq check
>D04569A7-2_A7Fd_EGFP-C_rev_B05_05_ABI24.ab1
    1 CACCCCGATG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG
   61 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT
  121 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC
  181 GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
  241 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
  301 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
  361 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
  421 GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
  481 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC
  541 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG
  601 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CTCGAATCAA
  661 GCTTATCGAT ACCGTCCGCC GCAGCGCAGA TGGTCGCGCC CGTGCCCCCC TATCTCGCGC
  721 CTCGCGTGGT GCGGTCCGGC TGGGCCGGCG GCGGCGCGGA CGCGACCAAG GTGGCCGGGA
  781 AGGGGAGTTT GCGGGGGACC GGCGAGTGAC GTCAGCGCGC CTTCAGTGCT GAGGCGGCGG
  841 TGGCGCGCGC CGCCAGGCGG GGGCGAAGGC ACTGTCCGCG GTGCTGAAGC TGGCAGTGCG
  901 CACGCGCCTC GCCGCATCCT GTTTCCCCTC CCCCTCTCTG ATAGGGATGC GCATTTGGGA
  961 AATGGGGGGG TTGGGGTGCT TGGTCCAGGT GGGGTCGGGG TCGGTCGTCA GGTAAGGCAC
 1021 CCCCCACCCC GCCTCATCCC TGGGTCCCTA TACCCCACTT TGCACCTCAT ACCGCAGGGC
 1081 CCTCTGGCAT GAACCCTTCC AGATATCTAG AACCCAGCTT CCTTGGTACA AAGTTGTTAC
 1141 GCGTGCCGGC CGCAGGATCT CCCTAATGAA TGATGGCAAC TCCCTCTTCT ATGCGCTGCG
1201 TAC
//
Primer: EGFP-C
Region: EGFP 3', BGH p(A), attB1, AAV2 ITR(R)
Sequence file: RDB15113_A7Fdc.seq check
>D04569A7-2_A7Fd_EGFP-C_H01_08_ABI08.ab1
    1 CCAGGGCCCC TCCTCGGGGC ATGGGGACGG AGCTGTACAA AGTAAATCGA ATTCCTGTGC
   61 CTTCTAAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA
  121 GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT
  181 AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA
  241 GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG GGATCCAGTC GACTGAATTG
  301 GTTCCTTTAA AGCCTGCTTT TTTGTACAAA CTTGTCACGT GCGGACCGAG CGGCCGCAGG
361 AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCAA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

2020.12.14

GNP_filter3_RDBDEP_html_201104.pl