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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2-CMV-GFP-BGHpA

AAV2 expression vector of GFP driven by the ubiquious promoter (CMV)

Catalog number RDB15112
Resource name pAAV2-CMV-GFP-BGHpA
Clone info. AAV2 expression vector of GFP driven by the ubiquious promoter (CMV), bovine growth hormone polyadenylation signal (BGHpA), see Fig. 1A of Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017.
Comment Expression was confrimed by the depositor with microscopic observation.
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Depositor|Developer Hioki, Hiroyuki |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15112 pAAV2-CMV-GFP-BGHpA DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB15112zzk01.seq provided by the depositor

            Original reference

            original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15112_A7Fdp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EGFP-N
            Region: EGFP 5', CMV pro, AAV2 ITR(L)
            Sequence file: RDB15112_A7Fda.seq check
            >D04569A6-1_A7Fd_EGFP-N_E01_05_ABI08.ab1
                1 GAAACCGGGG GGGGACAGCT CCTCGGCCCT TGCTCACCAT GGTGGCTCGA ATCAAGCTTA
               61 TCGATACCGT CGATCTGACG GTTCACTAAA CCAGCTCTGC TTATATAGAC CTCCCACCGT
              121 ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT TGGAAAGTCC
              181 CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT
              241 CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG CATCACCATG
              301 GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA TAAGGTCATG
              361 TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT
              421 GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT
              481 TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT TATTGACGTC
              541 AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG TTATGTAACG
              601 CGGAACTCCA TATATGGGCT ATGAACTAAT GACCCCGTAA TTGATTACTA TTAATAACTG
              661 ACCTCGAGAT ATCTAGACCC AGCTTTCTTG TACAAAGTGG TACGCGTGCG GCCGCAGGAA
            721 CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC
            //
            Primer: CMV_Forward
            Region: EGFP
            Sequence file: RDB15112_A7Fdb.seq check
            >D04569A6-1_A7Fd_CMV-Forward_G04_19_ABI24.ab1
                1 TATACACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
               61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCG ACGGTATCGA TAAGCTTGAT TCGAGCCACC
              121 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
              181 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
              241 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
              301 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
              361 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
              421 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
              481 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
              541 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
              601 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC
              661 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
              721 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
              781 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA
              841 ATCGAATTCC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
              901 CCCTTGACCC TGGAAGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG AAATTGCATC
            961 GCATTGTCTG AGTAGGTGTC ATTCTATTTC TGGGGCCTGG GTGTGGGGGG GGG
            //
            Primer: EGFP-C
            Region: EGFP 3', BGH p(A), attB1, AAV2 ITR(R)
            Sequence file: RDB15112_A7Fdc.seq check
            >D04569A6-1_A7Fd_EGFP-C_F01_06_ABI08.ab1
                1 TCAGGGTTCT TTTCGGGCAT GCACGAGCTG TACAAGTAAA TCGAATTCCT GTGCCTTCTA
               61 GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA
              121 CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC
              181 ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA
              241 GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGGGATCCA GTCGACTGAA TTGGTTCCTT
              301 TAAAGCCTGC TTTTTTGTAC AAACTTGTCA CGTGCGGACC GAGCGGCCGC AGGAACCCCT
            361 AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl