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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2-CMV-GFP-BGHpA (#RDB15112)

AAV2 expression vector of GFP driven by the ubiquious promoter (CMV)

Clone info. AAV2 expression vector of GFP driven by the ubiquious promoter (CMV), bovine growth hormone polyadenylation signal (BGHpA), see Fig. 1A of Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017.
Comment Expression was confrimed by the depositor with microscopic observation.
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Depositor|Developer Hioki, Hiroyuki |
 
Sequence (full) RDB15112zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15112 pAAV2-CMV-GFP-BGHpA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAAV2-CMV-GFP-BGHpA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15112).

Reference section:

Sohn, J., Takahashi, M., Okamoto, S., Ishida, Y., Furuta, T., Hioki, H., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15112_A7Fdp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N (Pr0027)
Region: EGFP 5', CMV pro, AAV2 ITR(L)
Sequence file: RDB15112_A7Fda.seq check
>D04569A6-1_A7Fd_EGFP-N_E01_05_ABI08.ab1
    1 GAAACCGGGG GGGGACAGCT CCTCGGCCCT TGCTCACCAT GGTGGCTCGA ATCAAGCTTA
   61 TCGATACCGT CGATCTGACG GTTCACTAAA CCAGCTCTGC TTATATAGAC CTCCCACCGT
  121 ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT TGGAAAGTCC
  181 CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT
  241 CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG CATCACCATG
  301 GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA TAAGGTCATG
  361 TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT
  421 GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT
  481 TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT TATTGACGTC
  541 AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG TTATGTAACG
  601 CGGAACTCCA TATATGGGCT ATGAACTAAT GACCCCGTAA TTGATTACTA TTAATAACTG
  661 ACCTCGAGAT ATCTAGACCC AGCTTTCTTG TACAAAGTGG TACGCGTGCG GCCGCAGGAA
721 CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC
//
Primer: CMV_Forward (Pr0016)
Region: EGFP
Sequence file: RDB15112_A7Fdb.seq check
>D04569A6-1_A7Fd_CMV-Forward_G04_19_ABI24.ab1
    1 TATACACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCG ACGGTATCGA TAAGCTTGAT TCGAGCCACC
  121 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
  181 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
  241 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
  301 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
  361 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
  421 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
  481 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
  541 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
  601 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC
  661 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
  721 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
  781 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA
  841 ATCGAATTCC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
  901 CCCTTGACCC TGGAAGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG AAATTGCATC
961 GCATTGTCTG AGTAGGTGTC ATTCTATTTC TGGGGCCTGG GTGTGGGGGG GGG
//
Primer: EGFP-C (Pr0025)
Region: EGFP 3', BGH p(A), attB1, AAV2 ITR（R）
Sequence file: RDB15112_A7Fdc.seq check
>D04569A6-1_A7Fd_EGFP-C_F01_06_ABI08.ab1
    1 TCAGGGTTCT TTTCGGGCAT GCACGAGCTG TACAAGTAAA TCGAATTCCT GTGCCTTCTA
   61 GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA
  121 CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC
  181 ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA
  241 GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGGGATCCA GTCGACTGAA TTGGTTCCTT
  301 TAAAGCCTGC TTTTTTGTAC AAACTTGTCA CGTGCGGACC GAGCGGCCGC AGGAACCCCT
361 AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]