RDB15111 - pAAV2-DEST(r)

Resource data sheet
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pAAV2-DEST(r)

Gateway(R) destination vector of AAV2 [reverse].

Catalog number	RDB15111
Resource name	pAAV2-DEST(r)
Clone info.	Gateway(R) destination vector of AAV2 [reverse].
Vector backbone	pAAV-MCS (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Ampicillin
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor	Hioki, Hiroyuki \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (John, J. et al. PLoS One, 12 (1): e0169611, 2017)

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Material Transfer Agreement (MTA) [Word]

Remarks ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (John, J. et al. PLoS One, 12 (1): e0169611, 2017)
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]
Remarks	((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (John, J. et al. PLoS One, 12 (1): e0169611, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]
備考	使用上の注意】このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15111	pAAV2-DEST(r)	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original	Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

Further references such as user reports and related articles (go to bottom)

Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB15111_A7Fdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: ccdB 3', attR1, AAV2 ITR（R） \| Primer: ccdB-C_F \| Sequence: RDB15111_A7Fda.seq
>D04569A5-1_A7Fd_ccdB-C_F_D01_04_ABI08.ab1 1 CTATTGGGGG GGGGGTGAAA ATCGGGGGGA TGAAGCTGGC GCATGATGAC CACCGATATG 61 GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA CCGCGAAAAT 121 GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG CTCCCTTATA 181 CACAGCCAGT CTGCAGGTCG ATACAGTAGA AATTACAGAA ACTTTATCAC GTTTAGTAAG 241 TATAGAGGCT GAAAATCCAG ATGAAGCCGA ACGACTTGTA AGAGAAAAGT ATAAGAGTTG 301 TGAAATTGTT CTTGATGCAG ATGATTTTCA GGACTATGAC ACTAGCGTAT ATGAATAGGT 361 AGATGTTTTT ATTTTGTCAC ACAAAAAAGA GGCTCGCACC TCTTTTTCTT ATTTCTTTTT 421 ATGATTTAAT ACGGCATTGA GGACAATAGC GAGTAGGCTG GATACGACGA TTCCGTTTGA 481 GAAGAACATT TGGAAGGCTG TCGGTCGACT AAGTTGGCAG CATCACCCGA AGAACATTTG 541 GAAGGCTGTC GGTCGACTAC AGGTCACTAA TACCATCTAA GTAGTTGATT CATAGTGACT 601 GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC TAATTTAATA 661 TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA CTTGTCACGT 721 GCGGACCGAG CGGCCGCAGG AACCCCTAGT GATGGGAGTT GGCCACTCCC TCTCTGCGCG 781 CTC //
Region: CmR 5', cat pro, attR2, AAV2 ITR (L) \| Primer: Cm_M_R \| Sequence: RDB15111_A7Fdb.seq
>D04569A5-1_A7Fd_Cm_M_R_C01_03_ABI08.ab1 1 TTAAGGAGGG GGAAACGTAT TCCCATTATT CACCAGCTCA CCGGTCTTTC ATTGGCCATA 61 CGGAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC 121 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG 181 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG 241 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT 301 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG 361 TTGGAACCTC TTACGTGCCG ATCAACGTCT CATTTTCGCC AAAAGTTGGC CCAGGGCTTC 421 CCGGTATCAA CAGGGACACC AGGATTTATT TATTCTGCGA AGTGATCTTC CGTCACAGGT 481 ATTTATTCGG CGCAAAGTGC GTCGGGTGAT GCTGCCAACT TAGTCGACTA CAGGTCACTA 541 ATACCATCTA AGTAGTTGAT TCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA 601 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT 661 CGTTCAGCTT TCTTGTACAA AAGTGGTACG CGTGCGGCCG CAGGAACCCC TAGTGATGGA 721 GTTGGCCCAC TCCCTCTCTG CGCGCTCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

2019.11.02

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