Resource data sheet
Prev.

pAAV2-DEST(r)

Gateway(R) destination vector of AAV2 [reverse].

Catalog number RDB15111
Resource name pAAV2-DEST(r)
Clone info. Gateway(R) destination vector of AAV2 [reverse].
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Hioki, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (John, J. et al. PLoS One, 12 (1): e0169611, 2017)
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Remarks ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15111 pAAV2-DEST(r) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15111_A7Fdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: ccdB 3', attR1, AAV2 ITR(R) | Primer: ccdB-C_F | Sequence: RDB15111_A7Fda.seq
>D04569A5-1_A7Fd_ccdB-C_F_D01_04_ABI08.ab1
    1 CTATTGGGGG GGGGGTGAAA ATCGGGGGGA TGAAGCTGGC GCATGATGAC CACCGATATG
   61 GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA CCGCGAAAAT
  121 GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG CTCCCTTATA
  181 CACAGCCAGT CTGCAGGTCG ATACAGTAGA AATTACAGAA ACTTTATCAC GTTTAGTAAG
  241 TATAGAGGCT GAAAATCCAG ATGAAGCCGA ACGACTTGTA AGAGAAAAGT ATAAGAGTTG
  301 TGAAATTGTT CTTGATGCAG ATGATTTTCA GGACTATGAC ACTAGCGTAT ATGAATAGGT
  361 AGATGTTTTT ATTTTGTCAC ACAAAAAAGA GGCTCGCACC TCTTTTTCTT ATTTCTTTTT
  421 ATGATTTAAT ACGGCATTGA GGACAATAGC GAGTAGGCTG GATACGACGA TTCCGTTTGA
  481 GAAGAACATT TGGAAGGCTG TCGGTCGACT AAGTTGGCAG CATCACCCGA AGAACATTTG
  541 GAAGGCTGTC GGTCGACTAC AGGTCACTAA TACCATCTAA GTAGTTGATT CATAGTGACT
  601 GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC TAATTTAATA
  661 TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA CTTGTCACGT
  721 GCGGACCGAG CGGCCGCAGG AACCCCTAGT GATGGGAGTT GGCCACTCCC TCTCTGCGCG
781 CTC
//
Region: CmR 5', cat pro, attR2, AAV2 ITR (L) | Primer: Cm_M_R | Sequence: RDB15111_A7Fdb.seq
>D04569A5-1_A7Fd_Cm_M_R_C01_03_ABI08.ab1
    1 TTAAGGAGGG GGAAACGTAT TCCCATTATT CACCAGCTCA CCGGTCTTTC ATTGGCCATA
   61 CGGAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
  121 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
  181 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
  241 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
  301 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG
  361 TTGGAACCTC TTACGTGCCG ATCAACGTCT CATTTTCGCC AAAAGTTGGC CCAGGGCTTC
  421 CCGGTATCAA CAGGGACACC AGGATTTATT TATTCTGCGA AGTGATCTTC CGTCACAGGT
  481 ATTTATTCGG CGCAAAGTGC GTCGGGTGAT GCTGCCAACT TAGTCGACTA CAGGTCACTA
  541 ATACCATCTA AGTAGTTGAT TCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA
  601 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT
  661 CGTTCAGCTT TCTTGTACAA AAGTGGTACG CGTGCGGCCG CAGGAACCCC TAGTGATGGA
721 GTTGGCCCAC TCCCTCTCTG CGCGCTCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

2019.11.02

GNP_filter3_RDBDEP_html_190927.pl