Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2-DEST(r) (#RDB15111)

Gateway(R) destination vector of AAV2 [reverse].

Clone info. Gateway(R) destination vector of AAV2 [reverse].
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Hioki, Hiroyuki |
 
Sequence (full) RDB15111zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
Remarks ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
備考 使用上の注意】このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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RDB15111 pAAV2-DEST(r) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAAV2-DEST(r) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15111).

Reference section:

Sohn, J., Takahashi, M., Okamoto, S., Ishida, Y., Furuta, T., Hioki, H., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15111_A7Fdp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ccdB-C_F (Pr0136)
Region: ccdB 3', attR1, AAV2 ITR（R）
Sequence file: RDB15111_A7Fda.seq check
>D04569A5-1_A7Fd_ccdB-C_F_D01_04_ABI08.ab1
    1 CTATTGGGGG GGGGGTGAAA ATCGGGGGGA TGAAGCTGGC GCATGATGAC CACCGATATG
   61 GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA CCGCGAAAAT
  121 GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG CTCCCTTATA
  181 CACAGCCAGT CTGCAGGTCG ATACAGTAGA AATTACAGAA ACTTTATCAC GTTTAGTAAG
  241 TATAGAGGCT GAAAATCCAG ATGAAGCCGA ACGACTTGTA AGAGAAAAGT ATAAGAGTTG
  301 TGAAATTGTT CTTGATGCAG ATGATTTTCA GGACTATGAC ACTAGCGTAT ATGAATAGGT
  361 AGATGTTTTT ATTTTGTCAC ACAAAAAAGA GGCTCGCACC TCTTTTTCTT ATTTCTTTTT
  421 ATGATTTAAT ACGGCATTGA GGACAATAGC GAGTAGGCTG GATACGACGA TTCCGTTTGA
  481 GAAGAACATT TGGAAGGCTG TCGGTCGACT AAGTTGGCAG CATCACCCGA AGAACATTTG
  541 GAAGGCTGTC GGTCGACTAC AGGTCACTAA TACCATCTAA GTAGTTGATT CATAGTGACT
  601 GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC TAATTTAATA
  661 TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA CTTGTCACGT
  721 GCGGACCGAG CGGCCGCAGG AACCCCTAGT GATGGGAGTT GGCCACTCCC TCTCTGCGCG
781 CTC
//
Primer: Cm_M_R (Pr0012)
Region: CmR 5', cat pro, attR2, AAV2 ITR (L)
Sequence file: RDB15111_A7Fdb.seq check
>D04569A5-1_A7Fd_Cm_M_R_C01_03_ABI08.ab1
    1 TTGAGGAGGG GGAAACGTAT TCCCATTATT CACCAGCTCA CCGGTCTTTC ATTGGCCATA
   61 CGGAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
  121 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
  181 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
  241 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
  301 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG
  361 TTGGAACCTC TTACGTGCCG ATCAACGTCT CATTTTCGCC AAAAGTTGGC CCAGGGCTTC
  421 CCGGTATCAA CAGGGACACC AGGATTTATT TATTCTGCGA AGTGATCTTC CGTCACAGGT
  481 ATTTATTCGG CGCAAAGTGC GTCGGGTGAT GCTGCCAACT TAGTCGACTA CAGGTCACTA
  541 ATACCATCTA AGTAGTTGAT TCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA
  601 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT
  661 CGTTCAGCTT TCTTGTACAA AGTGGTACGC GTGCGGCCGC AGGAACCCCT AGTGATGGAG
721 TTGGCCCACT CCCTCTCTGC GCGCTCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]