Resource data sheet
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pAAV2-DEST(f)

Gateway(R) destination vector of AAV2 [forword].

Catalog number RDB15110
Resource name pAAV2-DEST(f)
Clone info. Gateway(R) destination vector of AAV2 [forword].
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Hioki, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (John, J. et al. PLoS One, 12 (1): e0169611, 2017)
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Remarks ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


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RDB15110 pAAV2-DEST(f) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

Further references such as user reports and related articles (go to bottom)

Featured content

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Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15110_A9A8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: ccdB 3', attR1, AAV2 ITR(L) | Primer: ccdB-C_F | Sequence: RDB15110_A9A8a.seq
>D04569A4_A9A8_3_ccdB-C_F_A03_03_ABI24.ab1
    1 GCAGATACGG GTGGTGCATA TCGGGGATGA AGCTGGCGCA TGATGACCAC CGATATGGCC
   61 AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC
  121 ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC
  181 AGCCAGTCTG CAGGTCGATA CAGTAGAAAT TACAGAAACT TTATCACGTT TAGTAAGTAT
  241 AGAGGCTGAA AATCCAGATG AAGCCGAACG ACTTGTAAGA GAAAAGTATA AGAGTTGTGA
  301 AATTGTTCTT GATGCAGATG ATTTTCAGGA CTATGACACT AGCGTATATG AATAGGTAGA
  361 TGTTTTTATT TTGTCACACA AAAAAGAGGC TCGCACCTCT TTTTCTTATT TCTTTTTATG
  421 ATTTAATACG GCATTGAGGA CAATAGCGAG TAGGCTGGAT ACGACGATTC CGTTTGAGAA
  481 GAACATTTGG AAGGCTGTCG GTCGACTAAG TTGGCAGCAT CACCCGAAGA ACATTTGGAA
  541 GGCTGTCGGT CGACTACAGG TCACTAATAC CATCTAAGTA GTTGATTCAT AGTGACTGGA
  601 TATGTTGTGT TTTACAGTAT TATGTAGTCT GTTTTTTATG CAAAATCTAA TTTAATATAT
  661 TGATATTTAT ATCATTTTAC GTTTCTCGTT CAGCTTTTTT GTACAAACTT GTACGCGTGC
721 GGCCGCAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC TCTGCGCGCT C
//
Region: CmR 3', ccdB | Primer: Cm_C_F | Sequence: RDB15110_A9A8b.seq
>D04569A4_A9A8_3_Cm_C_F_B03_06_ABI24.ab1
    1 GGGCCTAGCG CGTGGATCCG GCTTACTaAA AGCCAGATAA CAGTATGCGT ATTTGCGCGC
   61 TGATTTTTGC GGTATAAGAA TATATACTGA TATGTATACC CGAAGTATGT CAAAAAGAGG
  121 TATGCTATGA AGCAGCGTAT TACAGTGACA GTTGACAGCG ACAGCTATCA GTTGCTCAAG
  181 GCATATATGA TGTCAATATC TCCGGTCTGG TAAGCACAAC CATGCAGAAT GAAGCCCGTC
  241 GTCTGCGTGC CGAACGCTGG AAAGCGGAAA ATCAGGAAGG GATGGCTGAG GTCGCCCGGT
  301 TTATTGAAAT GAACGGCTCT TTTGCTGACG AGAACAGGGG CTGGTGAAAT GCAGTTTAAG
  361 GTTTACACCT ATAAAAGAGA GAGCCGTTAT CGTCTGTTTG TGGATGTACA GAGTGATATT
  421 ATTGACACGC CCGGGCGACG GATGGTGATC CCCCTGGCCA GTGCACGTCT GCTGTCAGAT
  481 AAAGTCTCCC GTGAACTTTA CCCGGTGGTG CATATCGGGG ATGAAAGCTG GCGCATGATG
  541 ACCACCGATA TGGCCAGTGT GCCGGTCTCC GTTATCGGGG AAGAAGTGGC TGATCTCAGC
  601 CACCGCGAAA ATGACATCAA AAACGCCATT AACCTGATGT TCTGGGGAAT ATAAATGTCA
  661 GGCTCCCTTA TACACAGCCA GTCTGCAGGT CGATACAGTA GAAATTACAG AAACTTTATC
  721 ACGTTTAGTA AGTATAGAGG CTGAAAATCC AGATGAAGCC GAACGACTTG TAAGAGAAAA
  781 GTATAAGAGT TGTGAAATTG TTCTTGATGC AGATGATTTT CAGGACTATG ACACTAGCGT
  841 ATATGAATAG GTAGATGTTT TTTATTTTGT CACACAAAAA AGAGGCTCGC ACCTCTTTTT
  901 CTTATTTCTT TTTATGATTT AATACGGCAT TGAGGACAAT AGCGAGTAGG CTGGATACGA
  961 CGATTCCGTT TGAGAAGAAC ATTTGGGAAG CTGTCGGTCG ACTAAGTTGG CAGCATCACC
 1021 CGAAGACATT TGGAAGCTGT CGGTCGACTA CAGGTCACTA ATACCATCTA AGGTAGTGAT
 1081 CATAGTGACT GGATATGTGT GTTTACAGTA ATATGTAGTC TGGTTTTTAA TGCCAAAATT
1141 CTATACTTAA AAATAATATA ATTG
//
Region: CmR 5', cat pro, attR2, AAV2 ITR(R) | Primer: Cm_N_rev | Sequence: RDB15110_A9A8c.seq
>D04569A4_A9A8_3_Cm_N_rev_C03_09_ABI24.ab1
    1 CAAAGTCTAA AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG
   61 ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG
  121 CCCGGTAGTG ATCTTATTTC ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCGATCAACG
  181 TCTCATTTTC GCCAAAAGTT GGCCCAGGGC TTCCCGGTAT CAACAGGGAC ACCAGGATTT
  241 ATTTATTCTG CGAAGTGATC TTCCGTCACA GGTATTTATT CGGCGCAAAG TGCGTCGGGT
  301 GATGCTGCCA ACTTAGTCGA CTACAGGTCA CTAATACCAT CTAAGTAGTT GATTCATAGT
  361 GACTGGATAT GTTGTGTTTT ACAGTATTAT GTAGTCTGTT TTTTATGCAA AATCTAATTT
  421 AATATATTGA TATTTATATC ATTTTACGTT TCTCGTTCAG CTTTCTTGTA CAAAGTGGTC
  481 ACGTGCGGAC CGAGCGGCCG CAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC
541 GCGCTC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

2019.11.02

GNP_filter3_RDBDEP_html_190927.pl