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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2-DEST(f)

Gateway(R) destination vector of AAV2 [forword].

Catalog number RDB15110
Resource name pAAV2-DEST(f)
Clone info. Gateway(R) destination vector of AAV2 [forword].
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Hioki, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


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RDB15110 pAAV2-DEST(f) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15110_B0J8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Rfa-M_R
Region: attR1,AAV2 ITR
Sequence file: RDB15110_B0J8a.seq check
>15110_15110_B0J8_1_Rfa-M_R_H11_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGANT TGTGAATTGT TCTTGANGCA GATGANTTTC AGGACTATGA
   61 CACTAGCGTA TATGAATAGG TAGATGTTTT TATTTTGTCA CACAAAAAAG AGGCTCGCAC
  121 CTCTTTTTCT TATTTCTTTT TATGATTTAA TACGGCATTG AGGACAATAG CGAGTAGGCT
  181 GGATACGACG ATTCCGTTTG AGAAGAACAT TTGGAAGGCT GTCGGTCGAC TAAGTTGGCA
  241 GCATCACCCG AAGAACATTT GGAAGGCTGT CGGTCGACTA CAGGTCACTA ATACCATCTA
  301 AGTAGTTGAT TCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT
  361 TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT
  421 TTTTGTACAA ACTTGTACGC GTGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC
481 CCTCTCTGCG CGCTCNNNNN NNN
//
Primer: ccdB-C_F
Region: ccdB,attR1
Sequence file: RDB15110_B0J8b.seq check
>15110_15110_B0J8_1_ccdB-C_F_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCG NNNNNNNNNN CTGGCGCATG ATGACCNNCC GATATGGCCA
   61 GTGTGCCGGT CTCCNNNNNN NNGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA
  121 TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT GTCAGGCTCC CTTATACACA
  181 GCCAGTCTGC AGGTCGATAC AGTAGAAATT ACAGAAACTT TATCACGTTT AGTAAGTATA
  241 GAGGCTGAAA ATCCAGATGA AGCCGAACGA CTTGTAAGAG AAAAGTATAA GAGTTGTGAA
  301 ATTGTTCTTG ATGCAGATGA TTTTCAGGAC TATGACACTA GCGTATATGA ATAGGTAGAT
  361 GTTTTTATTT TGTCACACAA AAAAGAGGCT CGCACCTCTT TTTCTTATTT CTTTTTATGA
  421 TTTAATACGG CATTGAGGAC AATAGCGAGT AGGCTGGATA CGACGATTCC GTTTGAGAAG
  481 AACATTTGGA AGGCTGTCGG TCGACTAAGT TGGCAGCATC ACCCGAAGAA CATTTGGAAG
  541 GCTGTCGGTC GACTACAGGT CACTAATACC ATCTAAGTAG TTGATTCATA GTGACTGGAT
  601 ATGTTGTGTT TTACNGTATT ATGTAGTCTG TTTTTTANGC AAANNCTAAT TTAATATATT
  661 GANATTTATA TCATTTTACG TTTCTCNNTN NGCTTTTTTG NNCAAANNTG NNNNCNNGCN
721 GNNNNNNNNN N
//
Primer: Cm_M_R
Region: CmR,cat pro,attR2
Sequence file: RDB15110_B0J8c.seq check
>15110_15110_B0J8_1_Cm-M_R_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTCACCGTCT TTCNTTGCCN TACGGAATTC
   61 CGGATGAGCA TTCATCANGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT
  121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT
  181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC
  241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT
  301 CGATAACTCA AAAAATACGC CCGGTAGTGA TCTTATTTCA TTATGGTGAA AGTTGGAACC
  361 TCTTACGTGC CGATCAACGT CTCATTTTCG CCAAAAGTTG GCCCAGGGCT TCCCGGTATC
  421 AACAGGGACA CCAGGATTTA TTTATTCTGC GAAGTGATCT TCCGTCACAG GTATTTATTC
  481 GGCGCAAAGT GCGTCGGGTG ATGCTGCCAA CTTAGTCGAC TACAGGTCAC TAATACCATC
  541 TAAGTAGTTG ATTCATAGTG ACTGGATATG TTGTGTTTTA CAGTATTATG TAGTCTGTTT
  601 TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT CTCGTTCNGC
  661 TTTCNTGNAC AAAGTGGTCA CGTGCGNANC GANCNGNCCG CANGANNCCC NTANNGNTGN
721 ANNTNGNNCN ANNNNNNNNN N
//
Primer: Cm_N_rev
Region: cat pro,attR2,AAV2 ITR
Sequence file: RDB15110_B0J8d.seq check
>15110_15110_B0J8_1_Cm_N_rev_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNTCTTNNNG ATGCNNTTGG GANATATCAA CGGTGGTATA TCCAGTGATT
   61 TTTTTCTCCN TTNNNNNNNC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC
  121 GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC TTACGTGCCG ATCAACGTCT
  181 CATTTTCGCC AAAAGTTGGC CCAGGGCTTC CCGGTATCAA CAGGGACACC AGGATTTATT
  241 TATTCTGCGA AGTGATCTTC CGTCACAGGT ATTTATTCGG CGCAAAGTGC GTCGGGTGAT
  301 GCTGCCAACT TAGTCGACTA CAGGTCACTA ATACCATCTA AGTAGTTGAT TCATAGTGAC
  361 TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT CTAATTTAAT
  421 ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TCTTGTACAA AGTGGTCACG
  481 TGCGGACCGA GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG
541 NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

2020.11.06

GNP_filter3_RDBDEP_html_201104.pl