Sequencing and PCR primers for primer information.
Gateway(R) destination vector of AAV2 [forword].
| Catalog number | RDB15110 |
|---|---|
| Resource name | pAAV2-DEST(f) |
| Clone info. | Gateway(R) destination vector of AAV2 [forword]. |
| Vector backbone | pAAV-MCS (plasmid) |
| Size of vector backbone | 4.7 kb |
| Selectable markers | Ampicillin |
| Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
| Depositor | Hioki, Hiroyuki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (John, J. et al. PLoS One, 12 (1): e0169611, 2017) |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
| Remarks | ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15110 | pAAV2-DEST(f) | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Empty Backbone (English text) |
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| Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB15110_A9A8p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Region: ccdB 3', attR1, AAV2 ITR(L) | Primer: ccdB-C_F | Sequence: RDB15110_A9A8a.seq |
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>D04569A4_A9A8_3_ccdB-C_F_A03_03_ABI24.ab1 1 GCAGATACGG GTGGTGCATA TCGGGGATGA AGCTGGCGCA TGATGACCAC CGATATGGCC 61 AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC 121 ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC 181 AGCCAGTCTG CAGGTCGATA CAGTAGAAAT TACAGAAACT TTATCACGTT TAGTAAGTAT 241 AGAGGCTGAA AATCCAGATG AAGCCGAACG ACTTGTAAGA GAAAAGTATA AGAGTTGTGA 301 AATTGTTCTT GATGCAGATG ATTTTCAGGA CTATGACACT AGCGTATATG AATAGGTAGA 361 TGTTTTTATT TTGTCACACA AAAAAGAGGC TCGCACCTCT TTTTCTTATT TCTTTTTATG 421 ATTTAATACG GCATTGAGGA CAATAGCGAG TAGGCTGGAT ACGACGATTC CGTTTGAGAA 481 GAACATTTGG AAGGCTGTCG GTCGACTAAG TTGGCAGCAT CACCCGAAGA ACATTTGGAA 541 GGCTGTCGGT CGACTACAGG TCACTAATAC CATCTAAGTA GTTGATTCAT AGTGACTGGA 601 TATGTTGTGT TTTACAGTAT TATGTAGTCT GTTTTTTATG CAAAATCTAA TTTAATATAT 661 TGATATTTAT ATCATTTTAC GTTTCTCGTT CAGCTTTTTT GTACAAACTT GTACGCGTGC 721 GGCCGCAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC TCTGCGCGCT C // |
| Region: CmR 3', ccdB | Primer: Cm_C_F | Sequence: RDB15110_A9A8b.seq |
|
>D04569A4_A9A8_3_Cm_C_F_B03_06_ABI24.ab1 1 GGGCCTAGCG CGTGGATCCG GCTTACTaAA AGCCAGATAA CAGTATGCGT ATTTGCGCGC 61 TGATTTTTGC GGTATAAGAA TATATACTGA TATGTATACC CGAAGTATGT CAAAAAGAGG 121 TATGCTATGA AGCAGCGTAT TACAGTGACA GTTGACAGCG ACAGCTATCA GTTGCTCAAG 181 GCATATATGA TGTCAATATC TCCGGTCTGG TAAGCACAAC CATGCAGAAT GAAGCCCGTC 241 GTCTGCGTGC CGAACGCTGG AAAGCGGAAA ATCAGGAAGG GATGGCTGAG GTCGCCCGGT 301 TTATTGAAAT GAACGGCTCT TTTGCTGACG AGAACAGGGG CTGGTGAAAT GCAGTTTAAG 361 GTTTACACCT ATAAAAGAGA GAGCCGTTAT CGTCTGTTTG TGGATGTACA GAGTGATATT 421 ATTGACACGC CCGGGCGACG GATGGTGATC CCCCTGGCCA GTGCACGTCT GCTGTCAGAT 481 AAAGTCTCCC GTGAACTTTA CCCGGTGGTG CATATCGGGG ATGAAAGCTG GCGCATGATG 541 ACCACCGATA TGGCCAGTGT GCCGGTCTCC GTTATCGGGG AAGAAGTGGC TGATCTCAGC 601 CACCGCGAAA ATGACATCAA AAACGCCATT AACCTGATGT TCTGGGGAAT ATAAATGTCA 661 GGCTCCCTTA TACACAGCCA GTCTGCAGGT CGATACAGTA GAAATTACAG AAACTTTATC 721 ACGTTTAGTA AGTATAGAGG CTGAAAATCC AGATGAAGCC GAACGACTTG TAAGAGAAAA 781 GTATAAGAGT TGTGAAATTG TTCTTGATGC AGATGATTTT CAGGACTATG ACACTAGCGT 841 ATATGAATAG GTAGATGTTT TTTATTTTGT CACACAAAAA AGAGGCTCGC ACCTCTTTTT 901 CTTATTTCTT TTTATGATTT AATACGGCAT TGAGGACAAT AGCGAGTAGG CTGGATACGA 961 CGATTCCGTT TGAGAAGAAC ATTTGGGAAG CTGTCGGTCG ACTAAGTTGG CAGCATCACC 1021 CGAAGACATT TGGAAGCTGT CGGTCGACTA CAGGTCACTA ATACCATCTA AGGTAGTGAT 1081 CATAGTGACT GGATATGTGT GTTTACAGTA ATATGTAGTC TGGTTTTTAA TGCCAAAATT 1141 CTATACTTAA AAATAATATA ATTG // |
| Region: CmR 5', cat pro, attR2, AAV2 ITR（R） | Primer: Cm_N_rev | Sequence: RDB15110_A9A8c.seq |
|
>D04569A4_A9A8_3_Cm_N_rev_C03_09_ABI24.ab1 1 CAAAGTCTAA AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG 61 ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG 121 CCCGGTAGTG ATCTTATTTC ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCGATCAACG 181 TCTCATTTTC GCCAAAAGTT GGCCCAGGGC TTCCCGGTAT CAACAGGGAC ACCAGGATTT 241 ATTTATTCTG CGAAGTGATC TTCCGTCACA GGTATTTATT CGGCGCAAAG TGCGTCGGGT 301 GATGCTGCCA ACTTAGTCGA CTACAGGTCA CTAATACCAT CTAAGTAGTT GATTCATAGT 361 GACTGGATAT GTTGTGTTTT ACAGTATTAT GTAGTCTGTT TTTTATGCAA AATCTAATTT 421 AATATATTGA TATTTATATC ATTTTACGTT TCTCGTTCAG CTTTCTTGTA CAAAGTGGTC 481 ACGTGCGGAC CGAGCGGCCG CAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC 541 GCGCTC // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.11.02
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