Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAAV2-DEST(f)

Gateway(R) destination vector of AAV2 [forword].

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	使用上の注意】このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15110_B0J8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Rfa-M_R Region: attR1,AAV2 ITR Sequence file: RDB15110_B0J8a.seq
>15110_15110_B0J8_1_Rfa-M_R_H11_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNGANT TGTGAATTGT TCTTGANGCA GATGANTTTC AGGACTATGA    61 CACTAGCGTA TATGAATAGG TAGATGTTTT TATTTTGTCA CACAAAAAAG AGGCTCGCAC   121 CTCTTTTTCT TATTTCTTTT TATGATTTAA TACGGCATTG AGGACAATAG CGAGTAGGCT   181 GGATACGACG ATTCCGTTTG AGAAGAACAT TTGGAAGGCT GTCGGTCGAC TAAGTTGGCA   241 GCATCACCCG AAGAACATTT GGAAGGCTGT CGGTCGACTA CAGGTCACTA ATACCATCTA   301 AGTAGTTGAT TCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT   361 TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT   421 TTTTGTACAA ACTTGTACGC GTGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC 481 CCTCTCTGCG CGCTCNNNNN NNN //
Primer: ccdB-C_F Region: ccdB,attR1 Sequence file: RDB15110_B0J8b.seq
>15110_15110_B0J8_1_ccdB-C_F_B06_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCG NNNNNNNNNN CTGGCGCATG ATGACCNNCC GATATGGCCA    61 GTGTGCCGGT CTCCNNNNNN NNGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA   121 TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT GTCAGGCTCC CTTATACACA   181 GCCAGTCTGC AGGTCGATAC AGTAGAAATT ACAGAAACTT TATCACGTTT AGTAAGTATA   241 GAGGCTGAAA ATCCAGATGA AGCCGAACGA CTTGTAAGAG AAAAGTATAA GAGTTGTGAA   301 ATTGTTCTTG ATGCAGATGA TTTTCAGGAC TATGACACTA GCGTATATGA ATAGGTAGAT   361 GTTTTTATTT TGTCACACAA AAAAGAGGCT CGCACCTCTT TTTCTTATTT CTTTTTATGA   421 TTTAATACGG CATTGAGGAC AATAGCGAGT AGGCTGGATA CGACGATTCC GTTTGAGAAG   481 AACATTTGGA AGGCTGTCGG TCGACTAAGT TGGCAGCATC ACCCGAAGAA CATTTGGAAG   541 GCTGTCGGTC GACTACAGGT CACTAATACC ATCTAAGTAG TTGATTCATA GTGACTGGAT   601 ATGTTGTGTT TTACNGTATT ATGTAGTCTG TTTTTTANGC AAANNCTAAT TTAATATATT   661 GANATTTATA TCATTTTACG TTTCTCNNTN NGCTTTTTTG NNCAAANNTG NNNNCNNGCN 721 GNNNNNNNNN N //
Primer: Cm_M_R Region: CmR,cat pro,attR2 Sequence file: RDB15110_B0J8c.seq
>15110_15110_B0J8_1_Cm-M_R_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTCACCGTCT TTCNTTGCCN TACGGAATTC    61 CGGATGAGCA TTCATCANGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT   121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT   181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC   241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT   301 CGATAACTCA AAAAATACGC CCGGTAGTGA TCTTATTTCA TTATGGTGAA AGTTGGAACC   361 TCTTACGTGC CGATCAACGT CTCATTTTCG CCAAAAGTTG GCCCAGGGCT TCCCGGTATC   421 AACAGGGACA CCAGGATTTA TTTATTCTGC GAAGTGATCT TCCGTCACAG GTATTTATTC   481 GGCGCAAAGT GCGTCGGGTG ATGCTGCCAA CTTAGTCGAC TACAGGTCAC TAATACCATC   541 TAAGTAGTTG ATTCATAGTG ACTGGATATG TTGTGTTTTA CAGTATTATG TAGTCTGTTT   601 TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT CTCGTTCNGC   661 TTTCNTGNAC AAAGTGGTCA CGTGCGNANC GANCNGNCCG CANGANNCCC NTANNGNTGN 721 ANNTNGNNCN ANNNNNNNNN N //
Primer: Cm_N_rev Region: cat pro,attR2,AAV2 ITR Sequence file: RDB15110_B0J8d.seq
>15110_15110_B0J8_1_Cm_N_rev_C06_09_ABI24.ab1     1 NNNNNNNNNN NNTCTTNNNG ATGCNNTTGG GANATATCAA CGGTGGTATA TCCAGTGATT    61 TTTTTCTCCN TTNNNNNNNC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC   121 GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC TTACGTGCCG ATCAACGTCT   181 CATTTTCGCC AAAAGTTGGC CCAGGGCTTC CCGGTATCAA CAGGGACACC AGGATTTATT   241 TATTCTGCGA AGTGATCTTC CGTCACAGGT ATTTATTCGG CGCAAAGTGC GTCGGGTGAT   301 GCTGCCAACT TAGTCGACTA CAGGTCACTA ATACCATCTA AGTAGTTGAT TCATAGTGAC   361 TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT CTAATTTAAT   421 ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TCTTGTACAA AGTGGTCACG   481 TGCGGACCGA GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG 541 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.11.06

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