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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2-DEST(f)

Gateway(R) destination vector of AAV2 [forword].

Catalog number RDB15110
Resource name pAAV2-DEST(f)
Clone info. Gateway(R) destination vector of AAV2 [forword].
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Hioki, Hiroyuki |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 使用上の注意】このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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            RDB15110 pAAV2-DEST(f) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB15110zzk02.seq provided by the depositor

            Original reference

            original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15110_B0J8p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Rfa-M_R
            Region: attR1,AAV2 ITR
            Sequence file: RDB15110_B0J8a.seq check
            >15110_15110_B0J8_1_Rfa-M_R_H11_23_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGANT TGTGAATTGT TCTTGANGCA GATGANTTTC AGGACTATGA
               61 CACTAGCGTA TATGAATAGG TAGATGTTTT TATTTTGTCA CACAAAAAAG AGGCTCGCAC
              121 CTCTTTTTCT TATTTCTTTT TATGATTTAA TACGGCATTG AGGACAATAG CGAGTAGGCT
              181 GGATACGACG ATTCCGTTTG AGAAGAACAT TTGGAAGGCT GTCGGTCGAC TAAGTTGGCA
              241 GCATCACCCG AAGAACATTT GGAAGGCTGT CGGTCGACTA CAGGTCACTA ATACCATCTA
              301 AGTAGTTGAT TCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT
              361 TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT
              421 TTTTGTACAA ACTTGTACGC GTGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC
            481 CCTCTCTGCG CGCTCNNNNN NNN
            //
            Primer: ccdB-C_F
            Region: ccdB,attR1
            Sequence file: RDB15110_B0J8b.seq check
            >15110_15110_B0J8_1_ccdB-C_F_B06_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNCG NNNNNNNNNN CTGGCGCATG ATGACCNNCC GATATGGCCA
               61 GTGTGCCGGT CTCCNNNNNN NNGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA
              121 TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT GTCAGGCTCC CTTATACACA
              181 GCCAGTCTGC AGGTCGATAC AGTAGAAATT ACAGAAACTT TATCACGTTT AGTAAGTATA
              241 GAGGCTGAAA ATCCAGATGA AGCCGAACGA CTTGTAAGAG AAAAGTATAA GAGTTGTGAA
              301 ATTGTTCTTG ATGCAGATGA TTTTCAGGAC TATGACACTA GCGTATATGA ATAGGTAGAT
              361 GTTTTTATTT TGTCACACAA AAAAGAGGCT CGCACCTCTT TTTCTTATTT CTTTTTATGA
              421 TTTAATACGG CATTGAGGAC AATAGCGAGT AGGCTGGATA CGACGATTCC GTTTGAGAAG
              481 AACATTTGGA AGGCTGTCGG TCGACTAAGT TGGCAGCATC ACCCGAAGAA CATTTGGAAG
              541 GCTGTCGGTC GACTACAGGT CACTAATACC ATCTAAGTAG TTGATTCATA GTGACTGGAT
              601 ATGTTGTGTT TTACNGTATT ATGTAGTCTG TTTTTTANGC AAANNCTAAT TTAATATATT
              661 GANATTTATA TCATTTTACG TTTCTCNNTN NGCTTTTTTG NNCAAANNTG NNNNCNNGCN
            721 GNNNNNNNNN N
            //
            Primer: Cm_M_R
            Region: CmR,cat pro,attR2
            Sequence file: RDB15110_B0J8c.seq check
            >15110_15110_B0J8_1_Cm-M_R_H02_23_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTCACCGTCT TTCNTTGCCN TACGGAATTC
               61 CGGATGAGCA TTCATCANGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT
              121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT
              181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC
              241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT
              301 CGATAACTCA AAAAATACGC CCGGTAGTGA TCTTATTTCA TTATGGTGAA AGTTGGAACC
              361 TCTTACGTGC CGATCAACGT CTCATTTTCG CCAAAAGTTG GCCCAGGGCT TCCCGGTATC
              421 AACAGGGACA CCAGGATTTA TTTATTCTGC GAAGTGATCT TCCGTCACAG GTATTTATTC
              481 GGCGCAAAGT GCGTCGGGTG ATGCTGCCAA CTTAGTCGAC TACAGGTCAC TAATACCATC
              541 TAAGTAGTTG ATTCATAGTG ACTGGATATG TTGTGTTTTA CAGTATTATG TAGTCTGTTT
              601 TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT CTCGTTCNGC
              661 TTTCNTGNAC AAAGTGGTCA CGTGCGNANC GANCNGNCCG CANGANNCCC NTANNGNTGN
            721 ANNTNGNNCN ANNNNNNNNN N
            //
            Primer: Cm_N_rev
            Region: cat pro,attR2,AAV2 ITR
            Sequence file: RDB15110_B0J8d.seq check
            >15110_15110_B0J8_1_Cm_N_rev_C06_09_ABI24.ab1
                1 NNNNNNNNNN NNTCTTNNNG ATGCNNTTGG GANATATCAA CGGTGGTATA TCCAGTGATT
               61 TTTTTCTCCN TTNNNNNNNC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC
              121 GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC TTACGTGCCG ATCAACGTCT
              181 CATTTTCGCC AAAAGTTGGC CCAGGGCTTC CCGGTATCAA CAGGGACACC AGGATTTATT
              241 TATTCTGCGA AGTGATCTTC CGTCACAGGT ATTTATTCGG CGCAAAGTGC GTCGGGTGAT
              301 GCTGCCAACT TAGTCGACTA CAGGTCACTA ATACCATCTA AGTAGTTGAT TCATAGTGAC
              361 TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT CTAATTTAAT
              421 ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TCTTGTACAA AGTGGTCACG
              481 TGCGGACCGA GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG
            541 NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

            2022.03.03

            GNP_filter3_RDBDEP_html_220225.pl