Resource data sheet
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pAAV2-DEST(f) (#RDB15110)

Gateway(R) destination vector of AAV2 [forword].


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Sohn, J., PLoS One 12 (1): e0169611 (2017). PMID 28060929. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Gateway(R) destination vector of AAV2 [forword].
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Growth conditions LB+Amp, 30oC
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Hioki, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
Remarks ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
備考 使用上の注意】このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB15110 pAAV2-DEST(f) DNA solution

How to cite this biological resource

Materials & Methods section:

The pAAV2-DEST(f) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15110).

Reference section:

Sohn, J., Takahashi, M., Okamoto, S., Ishida, Y., Furuta, T., Hioki, H., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [PubMed] [Article] [RRC of NBRP]