RDB15110 - pAAV2-DEST(f)

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pAAV2-DEST(f) (#RDB15110)

Gateway(R) destination vector of AAV2 [forword].

Clone info.	Gateway(R) destination vector of AAV2 [forword].
Vector backbone	pAAV-MCS (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Ampicillin
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Hioki, Hiroyuki \|

Sequence (full)	RDB15110zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)

Remarks ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
Remarks	((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone. Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
備考	使用上の注意】このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15110	pAAV2-DEST(f)	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAAV2-DEST(f) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15110).

Reference section:

Sohn, J., Takahashi, M., Okamoto, S., Ishida, Y., Furuta, T., Hioki, H., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15110_B0J8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Rfa-M_R (Pr0899) Region: attR1,AAV2 ITR Sequence file: RDB15110_B0J8a.seq
>15110_15110_B0J8_1_Rfa-M_R_H11_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNGANT TGTGAATTGT TCTTGANGCA GATGANTTTC AGGACTATGA 61 CACTAGCGTA TATGAATAGG TAGATGTTTT TATTTTGTCA CACAAAAAAG AGGCTCGCAC 121 CTCTTTTTCT TATTTCTTTT TATGATTTAA TACGGCATTG AGGACAATAG CGAGTAGGCT 181 GGATACGACG ATTCCGTTTG AGAAGAACAT TTGGAAGGCT GTCGGTCGAC TAAGTTGGCA 241 GCATCACCCG AAGAACATTT GGAAGGCTGT CGGTCGACTA CAGGTCACTA ATACCATCTA 301 AGTAGTTGAT TCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT 361 TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT 421 TTTTGTACAA ACTTGTACGC GTGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC 481 CCTCTCTGCG CGCTCNNNNN NNN //
Primer: ccdB-C_F (Pr0136) Region: ccdB,attR1 Sequence file: RDB15110_B0J8b.seq
>15110_15110_B0J8_1_ccdB-C_F_B06_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNCG NNNNNNNNNN CTGGCGCATG ATGACCNNCC GATATGGCCA 61 GTGTGCCGGT CTCCNNNNNN NNGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA 121 TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT GTCAGGCTCC CTTATACACA 181 GCCAGTCTGC AGGTCGATAC AGTAGAAATT ACAGAAACTT TATCACGTTT AGTAAGTATA 241 GAGGCTGAAA ATCCAGATGA AGCCGAACGA CTTGTAAGAG AAAAGTATAA GAGTTGTGAA 301 ATTGTTCTTG ATGCAGATGA TTTTCAGGAC TATGACACTA GCGTATATGA ATAGGTAGAT 361 GTTTTTATTT TGTCACACAA AAAAGAGGCT CGCACCTCTT TTTCTTATTT CTTTTTATGA 421 TTTAATACGG CATTGAGGAC AATAGCGAGT AGGCTGGATA CGACGATTCC GTTTGAGAAG 481 AACATTTGGA AGGCTGTCGG TCGACTAAGT TGGCAGCATC ACCCGAAGAA CATTTGGAAG 541 GCTGTCGGTC GACTACAGGT CACTAATACC ATCTAAGTAG TTGATTCATA GTGACTGGAT 601 ATGTTGTGTT TTACNGTATT ATGTAGTCTG TTTTTTANGC AAANNCTAAT TTAATATATT 661 GANATTTATA TCATTTTACG TTTCTCNNTN NGCTTTTTTG NNCAAANNTG NNNNCNNGCN 721 GNNNNNNNNN N //
Primer: Cm_M_R (Pr0012) Region: CmR,cat pro,attR2 Sequence file: RDB15110_B0J8c.seq
>15110_15110_B0J8_1_Cm-M_R_H02_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTCACCGTCT TTCNTTGCCN TACGGAATTC 61 CGGATGAGCA TTCATCANGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT 121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT 181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC 241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT 301 CGATAACTCA AAAAATACGC CCGGTAGTGA TCTTATTTCA TTATGGTGAA AGTTGGAACC 361 TCTTACGTGC CGATCAACGT CTCATTTTCG CCAAAAGTTG GCCCAGGGCT TCCCGGTATC 421 AACAGGGACA CCAGGATTTA TTTATTCTGC GAAGTGATCT TCCGTCACAG GTATTTATTC 481 GGCGCAAAGT GCGTCGGGTG ATGCTGCCAA CTTAGTCGAC TACAGGTCAC TAATACCATC 541 TAAGTAGTTG ATTCATAGTG ACTGGATATG TTGTGTTTTA CAGTATTATG TAGTCTGTTT 601 TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT CTCGTTCNGC 661 TTTCNTGNAC AAAGTGGTCA CGTGCGNANC GANCNGNCCG CANGANNCCC NTANNGNTGN 721 ANNTNGNNCN ANNNNNNNNN N //
Primer: Cm_N_rev (Pr0014) Region: cat pro,attR2,AAV2 ITR Sequence file: RDB15110_B0J8d.seq
>15110_15110_B0J8_1_Cm_N_rev_C06_09_ABI24.ab1 1 NNNNNNNNNN NNTCTTNNNG ATGCNNTTGG GANATATCAA CGGTGGTATA TCCAGTGATT 61 TTTTTCTCCN TTNNNNNNNC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC 121 GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC TTACGTGCCG ATCAACGTCT 181 CATTTTCGCC AAAAGTTGGC CCAGGGCTTC CCGGTATCAA CAGGGACACC AGGATTTATT 241 TATTCTGCGA AGTGATCTTC CGTCACAGGT ATTTATTCGG CGCAAAGTGC GTCGGGTGAT 301 GCTGCCAACT TAGTCGACTA CAGGTCACTA ATACCATCTA AGTAGTTGAT TCATAGTGAC 361 TGGATATGTT GTGTTTTACA GTATTATGTA GTCTGTTTTT TATGCAAAAT CTAATTTAAT 421 ATATTGATAT TTATATCATT TTACGTTTCT CGTTCAGCTT TCTTGTACAA AGTGGTCACG 481 TGCGGACCGA GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG 541 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]

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