RDB15109 - pENTR1A-SYN-GFP-BGHpA(r)

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pENTR1A-SYN-GFP-BGHpA(r)

Gateway(R) entry vector of GFP expression unit driven by the neuron-specific promoter (human synapsin I (SYN)).


Catalog number	RDB15109
Resource name	pENTR1A-SYN-GFP-BGHpA(r)
Clone info.	Gateway(R) entry vector of GFP expression unit driven by the neuron-specific promoter (human synapsin I (SYN)), bovine growth hormone polyadenylation signal (BGHpA) [reverse], Gateway entry vector.
Vector backbone	pENTR1A (plasmid)
Size of vector backbone	2.7 kb
Selectable markers	Kanamycin
Gene/insert name	Human SYN1 genomic DNA
Depositor\|Developer	Hioki, Hiroyuki \|
Other clones in our bank	human SYN1 (NCBI Gene 6853) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)

Ordering Please visit Information of Request for Distribution.[link]
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]

Remarks
Please use Schedule A (GFP-A) additionally.
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally. Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。 Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15109	pENTR1A-SYN-GFP-BGHpA(r)	DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file	Nucleotide sequence provided by the depositor RDB15109zzk01.seq

Original reference

original	Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15109_A7Fdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: KanR-N_R Region: KanR 5', attL2, SYN pro, EGFP 5' Sequence file: RDB15109_A7Fda.seq
>D04569A3-2_A7Fd_KanR-N_R_H03_24_ABI24.ab1 1 TGGGGACATT TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTCGAC 61 GTTTCCCGTT GAATATGGCT CATAACACCC CTTGTATTAC TGTTTATGTA AGCAGACAGT 121 TTTATTGTTC ATGATGATAT ATTTTTATCT TGTGCAATGT AACATCAGAG ATTTTGAGAC 181 ACGGGCCAGA GCTGCAGCTG GATGGCAAAT AATGATTTTA TTTTGACTGA TAGTGACCTG 241 TTCGTTGCAA CAAATTGATA AGCAATGCTT TCTTATAATG CCAACTTTGT ACAAGAAAGC 301 TGGGTCTAGA TATCTCGAGG TCCTGCAGAG GGCCCTGCGT ATGAGTGCAA GTGGGTTTTA 361 GGACCAGGAT GAGGCGGGGT GGGGGTGCCT ACCTGACGAC CGACCCCGAC CCACTGGACA 421 AGCACCCAAC CCCCATTCCC CAAATTGCGC ATCCCCTATC AGAGAGGGGG AGGGGAAACA 481 GGATGCGGCG AGGCGCGTGC GCACTGCCAG CTTCAGCACC GCGGACAGTG CCTTCGCCCC 541 CGCCTGGCGG CGCGCGCCAC CGCCGCCTCA GCACTGAAGG CGCGCTGACG TCACTCGCCG 601 GTCCCCCGCA AACTCCCCTT CCCGGCCACC TTGGTCGCGT CCGCGCCGCC GCCGGCCCAG 661 CCGGACCGCA CCACGCGAGG CGCGAGATAG GGGGGCACGG GCGCGACCAT CTGCGCTGCG 721 GCGGACGGTA TCGATAAGCT TGATTCGAGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT 781 CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG 841 CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG 901 CACCACCGGC AAGCTGCCCG TGCCCTGACC CACCCTCGTG ACCACCCTGA CCTACGGCGT 961 GCAGTGCTTC AGCCCGCTAC CCCGACCACA TGAAAGCAGC ACGACTTTCT TTCCAAGTCC 1021 GCCATGCCCG AAGGCTAACG TCAGGAGCCG CAACCATCCT TTCATCAGGA CGATGTCAAC 1081 TTACTAGACC CGGCGCCCGG AG //
Primer: BGH_rev2 Region: BGH p(A), EGFP 3' Sequence file: RDB15109_A7Fdb.seq
>D04569A3-2_A7Ed_BGH_rev2_G03_21_ABI24.ab1 1 CAAGTCGCGG TGAGTAGCAC GGGGGCGGGG CAACAACGAG ATGGCTGGCG AACTGAGGAA 61 GGCACGGGAA TTCGATTTAC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG 121 TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG 181 ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT 241 TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA 301 AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG TGGCTGTTGT 361 AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA 421 GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT 481 TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT 541 TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT GAAGCACTGC ACGCCGTAGG 601 TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT 661 TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT 721 GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT 781 CGCCCTTGCT CACCATGGTG GCTCGAATCA AGCTTATCGA TACCGTCCGC CGCAGCGCAG 841 ATGGTCGCGC CCGTGCCCCC CCTATCTCGC GCCTCGCGTG GTGCGGTCCG GCTGGGCCGG 901 CGGCGGCGCG GACGCGACCA AAGTGGCCGG GAAGGGAGTT TGCGGGGGAC CGGCGAGTGA 961 CGTCAGCGCG CCTTCAGTGC TGAGCGGCGG TGGCGCGCGC CGCCAGCGGG GGGCGAAGCA 1021 CTGTCGCGTG CTGAGCTGGC CAATTGCGCA CGCGCTCGCG GCATCCTGAT CCCGTTCCCC 1081 CTCTCTGGAT TAGGGGATGG CGCCAATTAG A //
Primer: BGHpA_F Region: BGH p(A), attL1, rrnB T1 T2 ter Sequence file: RDB15109_A7Fdc.seq
>D04569A3_2_A7Fd_BGHpA_F_D01_10_ABI24.ab1 1 AGGGGGGGAA TGGGGTCATT CTATTCTGGG GGGGGTGGGG GTGGGGGCAG GGAACAGCAA 61 GGGGGGAGGG ATTGGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGG 121 GATCCAGTCG ACTGAATTGG TTCCTTTAAA GCCTGCTTTT TTGTACAAAG TTGGCATTAT 181 AAAAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT 241 CATTATTTGG GGCCCGAGCT TAAGTAACTA ACTAACAGGA AGAGTTTGTA GAAACGCAAA 301 AAGGCCATCC GTCAGGATGG CCTTCTGCTT AGTTTGATGC CTGGCAGTTT ATGGCGGGCG 361 TCCTGCCCGC CACCCTCCGG GCCGTTGCTT CACAACGTTC AAATCCGCTC CCGGCGGATT 421 TGTCCTACTC AGGAGAGCGT TCACCGACAA ACAACAGATA AAACGAAAGG CCCAGTCTTC 481 CGACTGAGCC TTTCGTTTTA TTTGATGCCT GGCAGTTCCC TACTCTCGCT TAGTAGTTAG 541 ACGTCCCCGA GATCCATGCT AGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 601 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 661 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGGCTCAAG 721 GTCAGAGGGT GGCGAAACCC GACAGGAACT ATAAAGGAAT ACCAAGGCCG TTTCCCCCTG 781 GGAAGCTCCC CTCGTGGCGC CTCCTCCTTG TTCCGGAACC CCTGTGCCGC CTTTTATACG 841 GAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

2021.05.03

GNP_filter3_RDBDEP_html_210401.pl