Please review the QC test results indicated by icon below as well as clone information before placing your order.

pENTR1A-SYN-GFP-BGHpA(r)

Gateway(R) entry vector of GFP expression unit driven by the neuron-specific promoter (human synapsin I (SYN)).

Sequence (full)	RDB15109zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15109_A7Fdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: KanR-N_R (Pr0530) Region: KanR 5', attL2, SYN pro, EGFP 5' Sequence file: RDB15109_A7Fda.seq
>D04569A3-2_A7Fd_KanR-N_R_H03_24_ABI24.ab1     1 TGGGGACATT TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTCGAC    61 GTTTCCCGTT GAATATGGCT CATAACACCC CTTGTATTAC TGTTTATGTA AGCAGACAGT   121 TTTATTGTTC ATGATGATAT ATTTTTATCT TGTGCAATGT AACATCAGAG ATTTTGAGAC   181 ACGGGCCAGA GCTGCAGCTG GATGGCAAAT AATGATTTTA TTTTGACTGA TAGTGACCTG   241 TTCGTTGCAA CAAATTGATA AGCAATGCTT TCTTATAATG CCAACTTTGT ACAAGAAAGC   301 TGGGTCTAGA TATCTCGAGG TCCTGCAGAG GGCCCTGCGT ATGAGTGCAA GTGGGTTTTA   361 GGACCAGGAT GAGGCGGGGT GGGGGTGCCT ACCTGACGAC CGACCCCGAC CCACTGGACA   421 AGCACCCAAC CCCCATTCCC CAAATTGCGC ATCCCCTATC AGAGAGGGGG AGGGGAAACA   481 GGATGCGGCG AGGCGCGTGC GCACTGCCAG CTTCAGCACC GCGGACAGTG CCTTCGCCCC   541 CGCCTGGCGG CGCGCGCCAC CGCCGCCTCA GCACTGAAGG CGCGCTGACG TCACTCGCCG   601 GTCCCCCGCA AACTCCCCTT CCCGGCCACC TTGGTCGCGT CCGCGCCGCC GCCGGCCCAG   661 CCGGACCGCA CCACGCGAGG CGCGAGATAG GGGGGCACGG GCGCGACCAT CTGCGCTGCG   721 GCGGACGGTA TCGATAAGCT TGATTCGAGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT   781 CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG   841 CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG   901 CACCACCGGC AAGCTGCCCG TGCCCTGACC CACCCTCGTG ACCACCCTGA CCTACGGCGT   961 GCAGTGCTTC AGCCCGCTAC CCCGACCACA TGAAAGCAGC ACGACTTTCT TTCCAAGTCC  1021 GCCATGCCCG AAGGCTAACG TCAGGAGCCG CAACCATCCT TTCATCAGGA CGATGTCAAC 1081 TTACTAGACC CGGCGCCCGG AG //
Primer: BGH_rev2 (Pr0606) Region: BGH p(A), EGFP 3' Sequence file: RDB15109_A7Fdb.seq
>D04569A3-2_A7Ed_BGH_rev2_G03_21_ABI24.ab1     1 CAAGTCGCGG TGAGTAGCAC GGGGGCGGGG CAACAACGAG ATGGCTGGCG AACTGAGGAA    61 GGCACGGGAA TTCGATTTAC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG   121 TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG   181 ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT   241 TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA   301 AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG TGGCTGTTGT   361 AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA   421 GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT   481 TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT   541 TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT GAAGCACTGC ACGCCGTAGG   601 TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT   661 TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT   721 GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT   781 CGCCCTTGCT CACCATGGTG GCTCGAATCA AGCTTATCGA TACCGTCCGC CGCAGCGCAG   841 ATGGTCGCGC CCGTGCCCCC CCTATCTCGC GCCTCGCGTG GTGCGGTCCG GCTGGGCCGG   901 CGGCGGCGCG GACGCGACCA AAGTGGCCGG GAAGGGAGTT TGCGGGGGAC CGGCGAGTGA   961 CGTCAGCGCG CCTTCAGTGC TGAGCGGCGG TGGCGCGCGC CGCCAGCGGG GGGCGAAGCA  1021 CTGTCGCGTG CTGAGCTGGC CAATTGCGCA CGCGCTCGCG GCATCCTGAT CCCGTTCCCC 1081 CTCTCTGGAT TAGGGGATGG CGCCAATTAG A //
Primer: BGHpA_F (Pr0373) Region: BGH p(A), attL1, rrnB T1 T2 ter Sequence file: RDB15109_A7Fdc.seq
>D04569A3_2_A7Fd_BGHpA_F_D01_10_ABI24.ab1     1 AGGGGGGGAA TGGGGTCATT CTATTCTGGG GGGGGTGGGG GTGGGGGCAG GGAACAGCAA    61 GGGGGGAGGG ATTGGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGG   121 GATCCAGTCG ACTGAATTGG TTCCTTTAAA GCCTGCTTTT TTGTACAAAG TTGGCATTAT   181 AAAAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT   241 CATTATTTGG GGCCCGAGCT TAAGTAACTA ACTAACAGGA AGAGTTTGTA GAAACGCAAA   301 AAGGCCATCC GTCAGGATGG CCTTCTGCTT AGTTTGATGC CTGGCAGTTT ATGGCGGGCG   361 TCCTGCCCGC CACCCTCCGG GCCGTTGCTT CACAACGTTC AAATCCGCTC CCGGCGGATT   421 TGTCCTACTC AGGAGAGCGT TCACCGACAA ACAACAGATA AAACGAAAGG CCCAGTCTTC   481 CGACTGAGCC TTTCGTTTTA TTTGATGCCT GGCAGTTCCC TACTCTCGCT TAGTAGTTAG   541 ACGTCCCCGA GATCCATGCT AGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA   601 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG   661 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGGCTCAAG   721 GTCAGAGGGT GGCGAAACCC GACAGGAACT ATAAAGGAAT ACCAAGGCCG TTTCCCCCTG   781 GGAAGCTCCC CTCGTGGCGC CTCCTCCTTG TTCCGGAACC CCTGTGCCGC CTTTTATACG 841 GAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl