Please review the QC test results indicated by icon below as well as clone information before placing your order.

pENTR1A-CMV-GFP-BGHpA(r)

Gateway(R) entry vector of GFP expression unit driven by the ubiquious promoter (CMV).

Sequence (full)	RDB15108zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15108_A7Fdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, EGFP 5' Sequence file: RDB15108_A7Fda.seq
>D04569A2-2_A7Ed_CMV-Forward_C03_09_ABI24.ab1     1 CATCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCGA CGGTATCGAT AAGCTTGATT CGAGCCACCA   121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG   181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG   241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC   301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC   361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT   421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG   481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA   541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG   601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG   661 ACCACTACCA GCAGAACACC CCCCATCGGC GACGCCCCGT GCTGCTGCCC GACACCACTA   721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCAACGAGA AGCCCGATCA CATGTCCTGC   781 TGAGTTCGTG ACCGCCGCCG GATCACCTCC TCGCATGACG AGCTGTACAG GTAATCGATT   841 CTGGTGGCCT TCTAGTGCAG CAATTCTGTT GTTGGCCCTC CCCGTGCTCG TGACCTGAGG   901 TCACCTCCCA TTGTCCTCCT ATAATGGAAA TGCATCGCAA TTGTCTGGAT TAGTCACTCA 961 TCTGCTGTTG CCAGACCAGC GC //
Primer: BGH_rev2 (Pr0606) Region: BGH p(A), EGFP 3' Sequence file: RDB15108_A7Fdb.seq
>D04569A2-2_A7Ed_BGH_rev2_D03_12_ABI24.ab1     1 CAGTCGCGTA GTAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCGAACT AGGAAGGCAC    61 AGGAATTCGA TTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG   121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG   181 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC   241 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC   301 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG   361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG   421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG   481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG   541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTAGGTCAGG   601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCAGG   661 GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG   721 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC   781 TTGCTCACCA TGGTGGCTCG AATCAAGCTT ATCGATACCG TCGATCTGAC GGTTCACTAA   841 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG   901 GGGCGGAGTT GTTACGACAT TTTTGGAAAG TCCCGTTGAT TTTGGGTGCC AAAACAAACT   961 CCCATTGACG TCAATGGGGG GTGGAGACTT GGTAAATCCC CGTGAGTCAA ACCCGCTATC  1021 CACGCCCATT GATGTACTGC CAAAACCGCA ATCACCATGG TATAGCGATG ACTACTACGT 1081 TAGATGGTAC CTGCCCTAGT ACGAAAGTTC CATTAAGGTC //
Primer: BGHpA_F (Pr0373) Region: BGH p(A), attL1, rrnB T1 T2 ter Sequence file: RDB15108_A7Fdc.seq
>D04569A2_2_A7Fd_BGHpA_F_B01_04_ABI24.ab1     1 GTAAAGGGGT AATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG    61 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGGGATCC AGTCGACTGA   121 ATTGGTTCCT TTAAAGCCTG CTTTTTTGTA CAAAGTTGGC ATTATAAAAA AGCATTGCTT   181 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGGGGCCC   241 GAGCTTAAGT AACTAACTAA CAGGAAGAGT TTGTAGAAAC GCAAAAAGGC CATCCGTCAG   301 GATGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTTATGGC GGGCGTCCTG CCCGCCACCC   361 TCCGGGCCGT TGCTTCACAA CGTTCAAATC CGCTCCCGGC GGATTTGTCC TACTCAGGAG   421 AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTCCGACT GAGCCTTTCG   481 TTTTATTTGA TGCCTGGCAG TTCCCTACTC TCGCTTAGTA GTTAGACGTC CCCGAGATCC   541 ATGCTAGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA   601 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT   661 AGGCTCCGCC CCCCTGACGA GCATCACAAA ATCGACGCTC AAGTCAGAGT GGCGAAACCC   721 GACAGACTAT AAAGATACCA GGCGTTCCCC TTGAGCTTCT TCGTGCGCCT CTCTGTCGAC 781 CTGCCCGCTT ACCGGGAATA CCTGTGTT //
Primer: CMV_en_R (Pr0173) Region: attL2, KanR Sequence file: RDB15108_A7Fdd.seq
>D04569A2-2_A7Ed_CMV_en_R_A03_03_ABI24.ab1     1 GACGTTTATG ATTACTATTA ATAACTGACC TCGAGATATC TAGACCCAGC TTTCTTGTAC    61 AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC   121 AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGCAGCTC TGGCCCGTGT CTCAAAATCT   181 CTGATGTTAC ATTGCACAAG ATAAAAATAT ATCATCATGA ACAATAAAAC TGTCTGCTTA   241 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG   301 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG   361 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT   421 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG   481 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC   541 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC   601 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTCC CTGCGCCGGT TGCATTCGAT   661 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC   721 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC   781 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT   841 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTTGA CGAGGGGAAA TTAATAGGTT   901 GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGGATCTTGC CCATCCTATG   961 GGAACTGCCT CGGTGAGTTT TTCTCCTTTC ATTTACAGAA ACGGCTTTTT TTCAAAAATA  1021 TGGGTTATTG ATTATTCCCT GATATGAATT AAATGGCCAG TTTCAATTTG AATGCTCGAT 1081 GAGTTTTCTG ATTCAGACTT GGCTTTAACT TGGCCTGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl