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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pENTR1A-CMV-GFP-BGHpA(r)

Gateway(R) entry vector of GFP expression unit driven by the ubiquious promoter (CMV).

Catalog number RDB15108
Resource name pENTR1A-CMV-GFP-BGHpA(r)
Clone info. Gateway(R) entry vector of GFP expression unit driven by the ubiquious promoter (CMV), bovine growth hormone polyadenylation signal (BGHpA) [reverse].
Vector backbone pENTR1A (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Kanamycin
Depositor|Developer Hioki, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15108 pENTR1A-CMV-GFP-BGHpA(r) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB15108zzk01.seq

Original reference

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15108_A7Fdp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, EGFP 5'
Sequence file: RDB15108_A7Fda.seq check
>D04569A2-2_A7Ed_CMV-Forward_C03_09_ABI24.ab1
    1 CATCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCGA CGGTATCGAT AAGCTTGATT CGAGCCACCA
  121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
  181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
  241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
  301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
  361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
  421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
  481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
  541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
  601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
  661 ACCACTACCA GCAGAACACC CCCCATCGGC GACGCCCCGT GCTGCTGCCC GACACCACTA
  721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCAACGAGA AGCCCGATCA CATGTCCTGC
  781 TGAGTTCGTG ACCGCCGCCG GATCACCTCC TCGCATGACG AGCTGTACAG GTAATCGATT
  841 CTGGTGGCCT TCTAGTGCAG CAATTCTGTT GTTGGCCCTC CCCGTGCTCG TGACCTGAGG
  901 TCACCTCCCA TTGTCCTCCT ATAATGGAAA TGCATCGCAA TTGTCTGGAT TAGTCACTCA
961 TCTGCTGTTG CCAGACCAGC GC
//
Primer: BGH_rev2
Region: BGH p(A), EGFP 3'
Sequence file: RDB15108_A7Fdb.seq check
>D04569A2-2_A7Ed_BGH_rev2_D03_12_ABI24.ab1
    1 CAGTCGCGTA GTAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCGAACT AGGAAGGCAC
   61 AGGAATTCGA TTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
  121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG
  181 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
  241 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC
  301 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG
  361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG
  421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
  481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG
  541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTAGGTCAGG
  601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCAGG
  661 GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG
  721 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC
  781 TTGCTCACCA TGGTGGCTCG AATCAAGCTT ATCGATACCG TCGATCTGAC GGTTCACTAA
  841 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG
  901 GGGCGGAGTT GTTACGACAT TTTTGGAAAG TCCCGTTGAT TTTGGGTGCC AAAACAAACT
  961 CCCATTGACG TCAATGGGGG GTGGAGACTT GGTAAATCCC CGTGAGTCAA ACCCGCTATC
 1021 CACGCCCATT GATGTACTGC CAAAACCGCA ATCACCATGG TATAGCGATG ACTACTACGT
1081 TAGATGGTAC CTGCCCTAGT ACGAAAGTTC CATTAAGGTC
//
Primer: BGHpA_F
Region: BGH p(A), attL1, rrnB T1 T2 ter
Sequence file: RDB15108_A7Fdc.seq check
>D04569A2_2_A7Fd_BGHpA_F_B01_04_ABI24.ab1
    1 GTAAAGGGGT AATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG
   61 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGGGATCC AGTCGACTGA
  121 ATTGGTTCCT TTAAAGCCTG CTTTTTTGTA CAAAGTTGGC ATTATAAAAA AGCATTGCTT
  181 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGGGGCCC
  241 GAGCTTAAGT AACTAACTAA CAGGAAGAGT TTGTAGAAAC GCAAAAAGGC CATCCGTCAG
  301 GATGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTTATGGC GGGCGTCCTG CCCGCCACCC
  361 TCCGGGCCGT TGCTTCACAA CGTTCAAATC CGCTCCCGGC GGATTTGTCC TACTCAGGAG
  421 AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTCCGACT GAGCCTTTCG
  481 TTTTATTTGA TGCCTGGCAG TTCCCTACTC TCGCTTAGTA GTTAGACGTC CCCGAGATCC
  541 ATGCTAGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA
  601 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT
  661 AGGCTCCGCC CCCCTGACGA GCATCACAAA ATCGACGCTC AAGTCAGAGT GGCGAAACCC
  721 GACAGACTAT AAAGATACCA GGCGTTCCCC TTGAGCTTCT TCGTGCGCCT CTCTGTCGAC
781 CTGCCCGCTT ACCGGGAATA CCTGTGTT
//
Primer: CMV_en_R
Region: attL2, KanR
Sequence file: RDB15108_A7Fdd.seq check
>D04569A2-2_A7Ed_CMV_en_R_A03_03_ABI24.ab1
    1 GACGTTTATG ATTACTATTA ATAACTGACC TCGAGATATC TAGACCCAGC TTTCTTGTAC
   61 AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC
  121 AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGCAGCTC TGGCCCGTGT CTCAAAATCT
  181 CTGATGTTAC ATTGCACAAG ATAAAAATAT ATCATCATGA ACAATAAAAC TGTCTGCTTA
  241 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG
  301 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG
  361 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT
  421 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG
  481 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC
  541 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC
  601 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTCC CTGCGCCGGT TGCATTCGAT
  661 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC
  721 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC
  781 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT
  841 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTTGA CGAGGGGAAA TTAATAGGTT
  901 GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGGATCTTGC CCATCCTATG
  961 GGAACTGCCT CGGTGAGTTT TTCTCCTTTC ATTTACAGAA ACGGCTTTTT TTCAAAAATA
 1021 TGGGTTATTG ATTATTCCCT GATATGAATT AAATGGCCAG TTTCAATTTG AATGCTCGAT
1081 GAGTTTTCTG ATTCAGACTT GGCTTTAACT TGGCCTGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

2021.05.03

GNP_filter3_RDBDEP_html_210401.pl