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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBSIISK-SYN-GFP-BGHpA (#RDB15107)

Expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)).

Clone info. Expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)), bovine growth hormone polyadenylation signal (BGHpA).
Vector backbone pBSIISK (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name Human SYN1 genomic DNA
Depositor|Developer Hioki, Hiroyuki |
 
Sequence (full) RDB15107zzk01.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15107 pBSIISK-SYN-GFP-BGHpA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBSIISK-SYN-GFP-BGHpA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15107).

Reference section:

Sohn, J., Takahashi, M., Okamoto, S., Ishida, Y., Furuta, T., Hioki, H., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15107_A7Fdp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410)
Region: SYN pro, EGFP 5'
Sequence file: RDB15107_A7Fda.seq check
>D04569A1-2_A7Ed_M13(-40)_G02_20_ABI24.ab1
    1 AGCCAAGCGC GCGCGTATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG
   61 AGGTCCTGCA GAGGGCCCTG CGTATGAGTG CAAGTGGGTT TTAGGACCAG GATGAGGCGG
  121 GGTGGGGGTG CCTACCTGAC GACCGACCCC GACCCACTGG ACAAGCACCC AACCCCCATT
  181 CCCCAAATTG CGCATCCCCT ATCAGAGAGG GGGAGGGGAA ACAGGATGCG GCGAGGCGCG
  241 TGCGCACTGC CAGCTTCAGC ACCGCGGACA GTGCCTTCGC CCCCGCCTGG CGGCGCGCGC
  301 CACCGCCGCC TCAGCACTGA AGGCGCGCTG ACGTCACTCG CCGGTCCCCC GCAAACTCCC
  361 CTTCCCGGCC ACCTTGGTCG CGTCCGCGCC GCCGCCGGCC CAGCCGGACC GCACCACGCG
  421 AGGCGCGAGA TAGGGGGGCA CGGGCGCGAC CATCTGCGCT GCGGCGGACG GTATCGATAA
  481 GCTTGATTCG AGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
  541 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG
  601 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
  661 CCGTGCCCTG CCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT
  721 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTTCTG CCATGCCCGA ATGCTACGAT
  781 CCAGGAGCGC ACATCTCTTC ATTGTACGAC GGCAACTACA GACTCGAGAA CCCACGGTCC
  841 AAAGGTTGGA AAGATTCTAT AGCGACACAC ACCTTGGTTG AATCCCCCAC TCCCAGCCCT
  901 CATAGCGATT CGACCTCTCA TGAGGATGAG AGGCGACACT CTCCTGTGGT GTCTCGAGAG
  961 CCTCGGATTA TCTAGCGCGC ACCACATCGG TCTTATATGT CTATGAGTCT GGCCAGGCAG
 1021 GATCGATGTG ATGCGTGCAG TACGATTCGC CATCAGTACG TACCGATGGT GCCGTACTTA
1081 GCAGCGCG
//
Primer: BGH_rev2 (Pr0606)
Region: EGFP 3'
Sequence file: RDB15107_A7Fdb.seq check
>D04569A1-2_A7Ed_BGH_rev2_H02_23_ABI24.ab1
    1 CGTCGGGGTG AGTAGCACGG GGGAGGGCAA ACAACAGATG GCTGGCGAAC TAGAAGGCAC
   61 AGGAATTCGG TTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
  121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG
  181 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
  241 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC
  301 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG
  361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG
  421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
  481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG
  541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTAGGTCAGG
  601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCAGG
  661 GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG
  721 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC
  781 TTGCTCACCA TGGTGGCTCG AATCAAGCTT ATCGATACCG TCCGCCGCAG CGCAGATGGT
  841 CGCGCCCGTG CCCCCCCTAT CTCGCGCCTC GCGTGGTGCG GTCCGGCTGG GCCGGGCGGC
  901 GGGCGCGGAC GCGACCAAAG TGGCCCGGGA ATGGGAGTTT GCGGGGGACC GGGCGAGTGA
  961 CGTCAGCGCG CCCTTCAGTG CTGAGCGCGG TGGCGCGCGC CGCCAGCGGG GGGGCGAAGC
 1021 ACTGTCCGCG GTGCTGAGCT GGCAATTGCG CACGCGCTCG CGCATCTGAT TCCCGTCCCC
1081 GTTCTTCTGG AATAGGGGGA TTGGCCGCCA AATG
//
Primer: EGFP-C (Pr0025)
Region: BGH p(A)
Sequence file: RDB15107_A7Fdc.seq check
>D04569A1-2_A7Fd_EGFP-C_A02_02_ABI24.ab1
    1 CAGGACTCTC GGGCATGGAC GAGCTGTACA AGTAAATCGA ATTCCTGTGC CTTCTAGTTG
   61 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC
  121 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC
  181 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG
  241 GCATGCTGGG GATGCGGTGG GCTCTATGGG GATCCACTAG TTCTAGAGCG GCCGCCACCG
  301 CGGTGGAGCT CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA
  361 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA
  421 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT
  481 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA
  541 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC
  601 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
  661 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
  721 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
  781 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
  841 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
  901 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
  961 AGCTCACGCT GTAGGTATCT CAGTTCGGGT GTAGGTCGTT TCGCTCCCAA GCTGGGGCTG
 1021 TGGTGCACGA ACCCCCCCCG TTCAGCCCGG ACGCCTGCCG CCTTATCCGG TACTATCGTC
1081 CATGAAGTTC GAACCCGGTT AAAGAACCAC CGAAACCTTT AAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]