Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBSIISK-SYN-GFP-BGHpA

Expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15107_A7Fdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Region: SYN pro, EGFP 5' Sequence file: RDB15107_A7Fda.seq
>D04569A1-2_A7Ed_M13(-40)_G02_20_ABI24.ab1     1 AGCCAAGCGC GCGCGTATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG    61 AGGTCCTGCA GAGGGCCCTG CGTATGAGTG CAAGTGGGTT TTAGGACCAG GATGAGGCGG   121 GGTGGGGGTG CCTACCTGAC GACCGACCCC GACCCACTGG ACAAGCACCC AACCCCCATT   181 CCCCAAATTG CGCATCCCCT ATCAGAGAGG GGGAGGGGAA ACAGGATGCG GCGAGGCGCG   241 TGCGCACTGC CAGCTTCAGC ACCGCGGACA GTGCCTTCGC CCCCGCCTGG CGGCGCGCGC   301 CACCGCCGCC TCAGCACTGA AGGCGCGCTG ACGTCACTCG CCGGTCCCCC GCAAACTCCC   361 CTTCCCGGCC ACCTTGGTCG CGTCCGCGCC GCCGCCGGCC CAGCCGGACC GCACCACGCG   421 AGGCGCGAGA TAGGGGGGCA CGGGCGCGAC CATCTGCGCT GCGGCGGACG GTATCGATAA   481 GCTTGATTCG AGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA   541 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG   601 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC   661 CCGTGCCCTG CCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT   721 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTTCTG CCATGCCCGA ATGCTACGAT   781 CCAGGAGCGC ACATCTCTTC ATTGTACGAC GGCAACTACA GACTCGAGAA CCCACGGTCC   841 AAAGGTTGGA AAGATTCTAT AGCGACACAC ACCTTGGTTG AATCCCCCAC TCCCAGCCCT   901 CATAGCGATT CGACCTCTCA TGAGGATGAG AGGCGACACT CTCCTGTGGT GTCTCGAGAG   961 CCTCGGATTA TCTAGCGCGC ACCACATCGG TCTTATATGT CTATGAGTCT GGCCAGGCAG  1021 GATCGATGTG ATGCGTGCAG TACGATTCGC CATCAGTACG TACCGATGGT GCCGTACTTA 1081 GCAGCGCG //
Primer: BGH_rev2 Region: EGFP 3' Sequence file: RDB15107_A7Fdb.seq
>D04569A1-2_A7Ed_BGH_rev2_H02_23_ABI24.ab1     1 CGTCGGGGTG AGTAGCACGG GGGAGGGCAA ACAACAGATG GCTGGCGAAC TAGAAGGCAC    61 AGGAATTCGG TTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG   121 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG   181 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC   241 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC   301 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG   361 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG   421 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG   481 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG   541 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTAGGTCAGG   601 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCAGG   661 GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG   721 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC   781 TTGCTCACCA TGGTGGCTCG AATCAAGCTT ATCGATACCG TCCGCCGCAG CGCAGATGGT   841 CGCGCCCGTG CCCCCCCTAT CTCGCGCCTC GCGTGGTGCG GTCCGGCTGG GCCGGGCGGC   901 GGGCGCGGAC GCGACCAAAG TGGCCCGGGA ATGGGAGTTT GCGGGGGACC GGGCGAGTGA   961 CGTCAGCGCG CCCTTCAGTG CTGAGCGCGG TGGCGCGCGC CGCCAGCGGG GGGGCGAAGC  1021 ACTGTCCGCG GTGCTGAGCT GGCAATTGCG CACGCGCTCG CGCATCTGAT TCCCGTCCCC 1081 GTTCTTCTGG AATAGGGGGA TTGGCCGCCA AATG //
Primer: EGFP-C Region: BGH p(A) Sequence file: RDB15107_A7Fdc.seq
>D04569A1-2_A7Fd_EGFP-C_A02_02_ABI24.ab1     1 CAGGACTCTC GGGCATGGAC GAGCTGTACA AGTAAATCGA ATTCCTGTGC CTTCTAGTTG    61 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC   121 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC   181 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG   241 GCATGCTGGG GATGCGGTGG GCTCTATGGG GATCCACTAG TTCTAGAGCG GCCGCCACCG   301 CGGTGGAGCT CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA   361 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA   421 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT   481 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA   541 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC   601 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG   661 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC   721 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC   781 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA   841 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC   901 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT   961 AGCTCACGCT GTAGGTATCT CAGTTCGGGT GTAGGTCGTT TCGCTCCCAA GCTGGGGCTG  1021 TGGTGCACGA ACCCCCCCCG TTCAGCCCGG ACGCCTGCCG CCTTATCCGG TACTATCGTC 1081 CATGAAGTTC GAACCCGGTT AAAGAACCAC CGAAACCTTT AAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.05.03

GNP_filter3_RDBDEP_html_210401.pl