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human Period3 (-3.1 - +0.2 kb)_luciferase/pENTR-1A (#RDB15084)

Gateway(R) entry vector with human Period 3 [-3.1 to +0.2 kb]-luciferase fragment.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Yamaguchi, A., Sci. Rep. 7 (1): 6824 (2017). PMID 28755004. [PubMed] [Article] [RRC of NBRP]
Publication Matsumura, R., J. Biol. Chem. 292 (39): 16081-16092 (2017). PMID 28821614. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Human PER3 promoter sequence (KpnI-XhoI 3.3 kb fragment from BAC clone “RP3-467L1”) followed by firefly luciferase gene (pGL3, Promega) is inserted into pENTR-1A (Invitrogen). Cloned fragment: 7781586 to 7784879(NC_000001.11, except 7784236A/G, 7784252A/G); relatively -3117 to +177, where +1 corresponds to 1 nt of NM_016831.1.
Vector backbone pENTR-1A (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Kan^r
Growth conditions LB+Kanamycin, 37oC
Gene/insert name human PER3 genomic Firefly luciferase cDNA
Depositor|Developer Akashi, Makoto |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamaguchi, A et al. Sci. Rep. 7(1): 6824, 2017)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamaguchi, A et al. Sci. Rep. 7(1): 6824, 2017)。
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee
RDB15084 human Period3 (-3.1 - +0.2 kb)_luciferase/pENTR-1A DNA solution

How to cite this biological resource

Materials & Methods section:

The human Period3 (-3.1 - +0.2 kb)_luciferase/pENTR-1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15084).

Reference section:

Yamaguchi, A., Matsumura, R., Matsuzaki, T., Nakamura, W., Node, K., Akashi, M., A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans. Sci. Rep. 7 (1): 6824 (2017). PMID 28755004. [PubMed] [Article] [RRC of NBRP]
Matsumura, R., Akashi, M., Multiple circadian transcriptional elements cooperatively regulate cell-autonomous transcriptional oscillation of Period3, a mammalian clock gene. J. Biol. Chem. 292 (39): 16081-16092 (2017). PMID 28821614. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yamaguchi, A., A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans. Sci. Rep. 7 (1): 6824 (2017). PMID 28755004. [PubMed] [Article] [RRC of NBRP]
original Matsumura, R., Multiple circadian transcriptional elements cooperatively regulate cell-autonomous transcriptional oscillation of Period3, a mammalian clock gene. J. Biol. Chem. 292 (39): 16081-16092 (2017). PMID 28821614. [PubMed] [Article] [RRC of NBRP]