Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

human Period3 (-3.1 - +0.2 kb)_luciferase/pENTR-1A (#RDB15084)

Gateway(R) entry vector with human Period 3 [-3.1 to +0.2 kb]-luciferase fragment.

Clone info. Human PER3 promoter sequence (KpnI-XhoI 3.3 kb fragment from BAC clone “RP3-467L1”) followed by firefly luciferase gene (pGL3, Promega) is inserted into pENTR-1A (Invitrogen). Cloned fragment: 7781586 to 7784879(NC_000001.11, except 7784236A/G, 7784252A/G); relatively -3117 to +177, where +1 corresponds to 1 nt of NM_016831.1.
Vector backbone pENTR-1A (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Kan^r
Gene/insert name human PER3 genomic Firefly luciferase cDNA
Depositor|Developer Akashi, Makoto |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yamaguchi, A et al. Sci. Rep. 7(1): 6824, 2017)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamaguchi, A et al. Sci. Rep. 7(1): 6824, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15084 human Period3 (-3.1 - +0.2 kb)_luciferase/pENTR-1A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human Period3 (-3.1 - +0.2 kb)_luciferase/pENTR-1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15084).

Reference section:

Yamaguchi, A., Matsumura, R., Matsuzaki, T., Nakamura, W., Node, K., Akashi, M., A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans. Sci. Rep. 7 (1): 6824 (2017). PMID 28755004. [link to RRC of NBRP]
Matsumura, R., Akashi, M., Multiple circadian transcriptional elements cooperatively regulate cell-autonomous transcriptional oscillation of Period3, a mammalian clock gene. J. Biol. Chem. 292 (39): 16081-16092 (2017). PMID 28821614. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15084_A8Ctp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: rrnBT1ter_F (Pr0488)
Region: rrnB T2 ter,attL1,insert 5'(hPer3 pro 5')
Sequence file: RDB15084_A8Cta.seq check
>D04733A2_A8Ct_2_rrnBT1ter_F_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNGTNNT NNNNNNNGCC CGGANGGTGG CGGGCAGGAN GCCCGCCATA
   61 AACTGCCAGG CATCAAACTA AGCAGAAGGC CATCCTGACG GATGGCCTTT TTGCGTTTCT
  121 ACAAACTCTT CCTGTTAGTT AGTTACTTAA GCTCGGGCCC CAAATAATGA TTTTATTTTG
  181 ACTGATAGTG ACCTGTTCGT TGCAACAAAT TGATAAGCAA TGCTTTTTTA TAATGCCAAC
  241 TTTGTACAAA AAAGCAGGCT TTAAAGGAAC CAATTCAGTC GACTGGATCC GGTACCCAGC
  301 ATTTCCGCAT ACCCCAGTGA ACAGAAGAAA ACCGCTCCCC TTGTGAAAAG TGCTTTTTAG
  361 TATAGGCGAT GAGGAGGAAA CTGGCTTTTA CACAATTACC AAAATTCAGG ATGTGGCTTT
  421 GGAGTTCCCT AAATAGATGG TCTTTCTAAA GTGATGTGCA GAGCCGACCC CGTCTGTGGT
  481 AGACTAGATG GCTGCTGGCA TGGACCCTGG GATAGATGAG TGGATAGGTG AGGGACATCC
  541 CCAGACACAT GGCTCTGTTC CTGTAGATCC TTTTTTCCTC ATGAATGTAT TTCCATTCTT
  601 ATCGTAGAAC CCCTAGGAAA CAATATAATA CGGGTGGAAG AGGACTTTTG CTTATGTTTA
  661 CAATAGGAAA ATCCAAGTCC CTTTCCCGAA TTACAGGTCC CACGATGATC TGGTCCTTTC
  721 TAGTTTTCTG ACTTCATGTC ACACGACTCT CCAGCTCTCT CTTCTCCAAG CAAGGTACTC
  781 CACCAAAGTA TAGCTGTTGA CATCGGAGAG ACTTGTGCCC ATGAGACTTT CNACATCAGG
  841 GTGTGGCTTG AANGCCAGAN TTAGCTATGA ATGAAAACAG GCAANGAAGC CCTGANGGAN
  901 AAATNNANCT GATAAAGTTT GNTTCNGTTT CTCATCAGCA AANTGACTTC TTTNCNANNN
961 NCTNNNGNNN NNANNTNCAG NNNNNNCNNG NNNNNNNNNN
//
Primer: KanR-N_R (Pr0530)
Region: KanR,attL2,insert 3'(SV40 pA,luc 3')
Sequence file: RDB15084_A8Ctb.seq check
>D04733A2_A8Ct_2_KanR-N_R_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN ATAAATCNGC ATCNNTGTTG GAATTTAATC GCGGCCTCGA
   61 CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG
  121 TTTTATTGTT CATGATGATA TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA
  181 CACGGGCCAG AGCTGCAGCT GGATGGCAAA TAATGATTTT ATTTTGACTG ATAGTGACCT
  241 GTTCGTTGCA ACAAATTGAT AAGCAATGCT TTCTTATAAT GCCAACTTTG TACAAGAAAG
  301 CTGGGTCTAG ATATCTCGAC GGATCCTTAT CGATTTTACC ACATTTGTAG AGGTTTTACT
  361 TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT
  421 TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA
  481 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA
  541 TGTATCTTAT CATGTCTGCT CGAAGCGGCC GGCCGCCCCG ACTCTAGAAT TACACGGCGA
  601 TCTTTCCGCC CTTCTTGGCC TTTATGAGGA TCTCTCTGAT TTTTCTTGCG TCGAGTTTTC
  661 CGGTAAGACC TTTCGGTACT TCGTCCACAA ACACAACTCC TCCGCGCAAC TTTTTCGCGG
  721 TTGTTACTTG ACTGGCGACG TAATCCACGA TCTCTTTTTC CGTCATCGTC TTTCCGTGCT
  781 CCAAAACAAC AACGGCGGCG GGAAGTTCAN CGGCGTCATC GTCGGGAAGA NCTGCGACAC
  841 CTGCGTCGAA NANGTTGGGG NGNTGGANCA AGANGGANTC CAATTCAGCG GGANCCACCT
  901 GATAGCCTTT GTACTTNATC ANANANTTCN NNCGGNCAAN GATGAAGANN TGNNCNTCTT
  961 CNTCCCNNNN ANCTATGTCT NNNNANNGNN GNNNNNCNTN CNTNNNCNNT NNANNNNNNN
1021 NGNNGCNTNN NNNANNNNNN NNNN
//
Primer: Luc-B_R (Pr0790)
Region: insert mid(luc 5',hPer3 pro 3')
Sequence file: RDB15084_A8Ctc.seq check
>D04733A2_A8Ct_2_Luc-B_R_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNT CTCTTNNTNG CCTTATGCAG TTGCTCTCCN GCGGTTCCNT
   61 CTTCCNGCGG ATAGAATGGC GCCGGGCCTT TCTTTATGTT TTTGGCGTCT TCCATGGTGG
  121 CTTTACCAAC AGTACCGGAA TGCCAAGCTT ACTTAGATCG CAGATCTCGA GGTCTCCGCG
  181 GGGCTCCACG TCGTGCTGGG ACGGCCCGCA GCCTGCTTCT CGCTCACTTT GCAGTCAGTG
  241 ACAGACACCG GTTTCTCACT CCGGTCCCCG CCATGGCGTT AGTGAGAACA GGCGGCCACC
  301 ACGAGCCAGG GGGTCACGCT CATCTCCGAG GAGCTTTTCC CTCCAACATT TTCAAAACCC
  361 GGAGCTCGCT CGACTTTCAG GACTCCGGCC TAGGGGGAGG AGACAAGGGT GACAAGGGAC
  421 AAATGGAACA GACAGTCCGA CGCCAGCGGC CCGGCTGGCT GGGGCGCCCC GACCGCGCGG
  481 TCCTCACCCC TGCCCCAACT TTCCCGCGCG CCGAGGGATG GGGCCCGGGG AGGCGGGTGG
  541 CTCGGAAGCG CCACGCTCCG GGGGCCCCCA GTACCGAGGG CAGGGCTCGC GGGGCTCCTC
  601 TGCCGCGCGC GAGTGCCCAG AACTCCCGCC CGGCCGCCCA GAACTCCCGC CCGGCCGCGC
  661 ACTCACCTGC TGCCCCCGCC CTTGGCCCCG CGCACAGCCG CTCGCCCCCG GGGCCGCCCT
  721 CGCGCAGTCT CGAGGCTCGC CGCGTGCCGG TCAGCAGCCG GCGCCGCGCC CCCTGCCGCC
  781 GCGTGACCGG CCCCGCCCCC GGGCCGCGCG ATTGNTCGTC GGGGCCGACT CCGCANGCCT
  841 CCATTGGCCC TGGGGCGGNG CGCGANCCGG TGCGCCCGGG TTATATNAGC CAGGGCGANG
  901 GGGGCGGGGC CCCCGGGGGC GCGTTACNTN ACGCGCGGGN NCNGNCGGNN GNNGGCGCNN
  961 GGNNNNTCCC ANNNNNCCNG CGGGNGGGNA GGGGNNGGGN NNNNCCNNNN NNNGNNNNNN
1021 NNNCNNNNNN NNNN
//
Primer: Luc_inner_R2 (Pr1286)
Region: insert mid(luc 5',hPer3 pro 3')
Sequence file: RDB15084_A8Ctd.seq check
>D04733A2_A8Ct_2_Luc inner_R2_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCTNNNGNA GGCAGTTCTA TNNNGGNAGA GCNNACACCT
   61 TTAGGCAGAC CAGTAGATCC AGAGGAGTTC ATGATCAGTG CAATTGTCTT GTCCCTATCG
  121 AAGGACTCTG GCACAAAATC GTATTCATTA AAACCGGGAG GTAGATGAGA TGTGACGAAC
  181 GTGTACATCG ACTGAAATCC CTGGTAATCC GTTTTAGAAT CCATGATAAT AATTTTTTGG
  241 ATGATTGGGA GCTTTTTTTG CACGTTCAAA ATTTTTTGCA ACCCCTTTTT GGAAACGAAC
  301 ACCACGGTAG GCTGCGAAAT GCCCATACTG TTGAGCAATT CACGTTCATT ATAAATGTCG
  361 TTCGCGGGCG CAACTGCAAC TCCGATAAAT AACGCGCCCA ACACCGGCAT AAAGAATTGA
  421 AGAGAGTTTT CACTGCATAC GACGATTCTG TGATTTGTAT TCAGCCCATA TCGTTTCATA
  481 GCTTCTGCCA ACCGAACGGA CATTTCGAAG TACTCAGCGT AAGTGATGTC CACCTCGATA
  541 TGTGCATCTG TAAAAGCAAT TGTTCCAGGA ACCAGGGCGT ATCTCTTCAT AGCCTTATGC
  601 AGTTGCTCTC CAGCGGTTCC ATCTTCCAGC GGATAGAATG GCGCCGGGCC TTTCTTTATG
  661 TTTTTGGCGT CTTCCATGGT GGCTTTACCA ACAGTACCGG AATGCCAAGC TTACTTAGAT
  721 CGCAGATCTC GAGGTCTCCG CGGGGCTCCA CGTCGTGCTG GGACGGNCCG CAGCCTGCTT
  781 CTCGCTCACT TTGCANTCAG TGACAGACAC CGGTTTCTCA CTCCGGTCCC CGCCNTGGCG
  841 TTAGTGAGAA CAGGCNGCCA CCACNAGCNA GGGGGTCACG CTCATCTNNN ANGAGCTTTT
  901 NCCTCCNACA TTTTTCNAAA NCCGGANCTC GCTCGANTTT TNNNNNANTN CNGGCCTNNN
  961 GGANGNAGNN NNGGNNNNNN ANNGNANNAA NNNNNNANAN AGNCCGANNN NNNNNGNCCC
1021 NNNNNNNNNN
//
Primer: pAxCALNLF1 (Pr0166)
Region: insert mid(luc 3')
Sequence file: RDB15084_A8Cte.seq check
>D04733A2_A8Ct_2_pAxCALNLF1_H04_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NAGCGGCCGG CCGCCCCGAC TCTNNAATTA
   61 CACGGCGATC TTTCCGCCCT TCTTGGCCTT TATGAGGATC TCTCTGATTT TTCTTGCGTC
  121 GAGTTTTCCG GTAAGACCTT TCGGTACTTC GTCCACAAAC ACAACTCCTC CGCGCAACTT
  181 TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC TCTTTTTCCG TCATCGTCTT
  241 TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG GCGTCATCGT CGGGAAGACC
  301 TGCGACACCT GCGTCGAAGA TGTTGGGGTG TTGGAGCAAG ATGGATTCCA ATTCAGCGGG
  361 AGCCACCTGA TAGCCTTTGT ACTTAATCAG AGACTTCAGG CGGTCAACGA TGAAGAAGTG
  421 TTCGTCTTCG TCCCAGTAAG CTATGTCTCC AGAATGTAGC CATCCATCCT TGTCAATCAA
  481 GGCGTTGGTC GCTTCCGGAT TGTTTACATA ACCGGACATA ATCATAGGAC CTCTCACACA
  541 CAGTTCGCCT CTTTGATTAA CGCCCAGCGT TTTCCCGGTA TCCAGATCCA CAACCTTCGC
  601 TTCAAAAAAT GGAACAACTT TACCGACCGC GCCCGGTTTA TCATCCCCCT CGGGTGTAAT
  661 CAGAATAGCT GATGTAGTCT CAGTGAGCCC ATATCCTTGC CTGATACCTG GCAGANGGAA
  721 CCTCTTGGCA ACCGCTTCCC CNACTTCCTT ANANANGGGA GCGCCACCAN AAGCNANTTT
  781 CNTGTAAATT NNNATAAATC GTATTTGTCA ATCANAGTGC TTTTTGGNNA NNNANGAGAN
  841 NAGGNNTGGC ACCNNCANCG CACTTTTGNA TCTTGTNATC NNNGAANNNT NCNTCANAAA
  901 CNNCTCTNNN TCAAATCTNT ACATTNAAGA CGANTNNNAA NTNCNNNNTN TCNAATNTNC
  961 NNANNGTANN NAACNTTNCN AAANCNNNGN NNNGANTGGN NNNNNNNNTN AAATCNNNNN
1021 TATCNNNNNN NGNNTTTGNN TNGNNNAAAA NNNNGNTCTN NNGNNNNNNN NNN
//
Primer: Luc inner_R1 (Pr1257)
Region: insert mid(luc mid)
Sequence file: RDB15084_A8Ctf.seq check
>D04733A2_A8Ct_2_Luc inner_R1_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTTTTCCN GGTNNCNNGA TCCNCAACCT TCGCTTCNAA
   61 AAATGGAACA ACTTTACCGA CCGCGCCCGG TTTATCATCC CCCTCGGGTG TAATCAGAAT
  121 AGCTGATGTA GTCTCAGTGA GCCCATATCC TTGCCTGATA CCTGGCAGAT GGAACCTCTT
  181 GGCAACCGCT TCCCCGACTT CCTTAGAGAG GGGAGCGCCA CCAGAAGCAA TTTCGTGTAA
  241 ATTAGATAAA TCGTATTTGT CAATCAGAGT GCTTTTGGCG AAGAAGGAGA ATAGGGTTGG
  301 CACCAGCAGC GCACTTTGAA TCTTGTAATC CTGAAGGCTC CTCAGAAACA GCTCTTCTTC
  361 AAATCTATAC ATTAAGACGA CTCGAAATCC ACATATCAAA TATCCGAGTG TAGTAAACAT
  421 TCCAAAACCG TGATGGAATG GAACAACACT TAAAATCGCA GTATCCGGAA TGATTTGATT
  481 GCCAAAAATA GGATCTCTGG CATGCGAGAA TCTCACGCAG GCAGTTCTAT GAGGCAGAGC
  541 GACACCTTTA GGCAGACCAG TAGATCCAGA GGAGTTCATG ATCAGTGCAA TTGTCTTGTC
  601 CCTATCGAAG GACTCTGGCA CAAAATCGTA TTCATTAAAA CCGGGAGGTA GATGAGATGT
  661 GACGAACGTG TACATCGACT GAAATCCCTG GTAATCCGTT TTAGAATCCA TGATAATAAT
  721 TTTTTGGATG ANTGGGAGCT TTTTTTGCAC GTTCAAAATT TTTTGCAACC CCTTTTTGGA
  781 AACGAACACC ACGGTAGGCT GCGAAATGCC CATACTGTTG AGCAATTCAC GTTCATTATA
  841 AATGTCGTTC GCGGGCGCNA CTGCAACTCC GANAAATAAC GCGCCCNACA CCGGCATAAA
  901 GAATTGAANA NAGTTTTCAN TGCATACGAC GATTCTGTGN NTTGNATTCA GCCNNTANNN
961 NNTCNTANNN NCTGNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamaguchi, A., A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans. Sci. Rep. 7 (1): 6824 (2017). PMID 28755004. [link to RRC of NBRP]
original Matsumura, R., Multiple circadian transcriptional elements cooperatively regulate cell-autonomous transcriptional oscillation of Period3, a mammalian clock gene. J. Biol. Chem. 292 (39): 16081-16092 (2017). PMID 28821614. [link to RRC of NBRP]