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pHA_RSB1D100p5

Expression vector of S. cerevisiae Rsb1 mutant.

Catalog number RDB15071
Resource name pHA_RSB1D100p5
Alternative name pHM23
Clone info. Expression vector of S. cerevisiae Rsb1 mutant lacking the central part of loop 5 region; 199 to 228th a.a), tagged with N-terminal 3xHA under the GAPDH (TDH3) promoter.
Expression was confirmed by the depositor with immunoblotting. For pAK162, refer Kihara, A. et al. J. Biol. Chem., 277(33): 30048-30054, 2002. PMID: 12034738.
Vector backbone pAK162 (plasmid)
Selectable markers Amp^r (E. coli), URA3 (S.c).
Gene/insert name S. cerevisiae RSB1 cDNA
Depositor Kihara, Akio |
 
Other clones in our bank S. cerevisiae RSB1 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makuta, H. et al. J. Biochem. 161(2), 207-213, 2017).
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RDB15071 pHA_RSB1D100p5 Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Makuta, H., Loop 5 region is important for the activity of the long-chain base transporter Rsb1. J. Biochem. 161 (2): 207-213 (2017). PMID 28175317.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Makuta, H., Loop 5 region is important for the activity of the long-chain base transporter Rsb1. J. Biochem. 161 (2): 207-213 (2017). PMID 28175317.

2019.07.17

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