Resource data sheet
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pHA_YLR046C (#RDB15070)

Expression vector of S. cerevisiae YLR046C.

Alternative name pHM27
Clone info. Expression vector of S. cerevisiae YLR046C, tagged with N-terminal 3xHA under the GAPDH (TDH3) promoter.
Comment Expression was confirmed by the depositor with immunoblotting. For pAK162, refer Kihara, A. et al. J. Biol. Chem., 277(33): 30048-30054, 2002. PMID: 12034738.
Vector backbone pAK162 (plasmid)
Selectable markers Amp^r (E. coli), URA3 (S.c).
Gene/insert name S. cerevisiae YLR046C cDNA
Depositor|Developer Kihara, Akio |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makuta, H. et al. J. Biochem. 161(2), 207-213, 2017).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makuta, H. et al. J. Biochem. 161(2), 207-213, 2017)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15070 pHA_YLR046C Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The pHA_YLR046C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15070).

Reference section:

Makuta, H., Obara, K., Kihara, A., Loop 5 region is important for the activity of the long-chain base transporter Rsb1. J. Biochem. 161 (2): 207-213 (2017). PMID 28175317. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Makuta, H., Loop 5 region is important for the activity of the long-chain base transporter Rsb1. J. Biochem. 161 (2): 207-213 (2017). PMID 28175317. [link to RRC of NBRP]