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pK178.CAG-loxP-stop-loxP-mir30 (GFP RNAi scramble control) (Supernova) (#RDB15059)

Cre-based Supernova series, a supernova-mediated RNAi enables sparsely labeled cell-specific gene knockdown without using genetically altered mice.

Alternative name pK178,
Supernova-mediated RNAi vectors
Clone info. Cre-based Supernova series, a supernova-mediated RNAi enables sparsely labeled cell-specific gene knockdown without using genetically altered mice. Cre-dependent GFP shRNA scramble control expression vector of loxP-stop-loxP-mir30 under the CAG promoter. Please refer Fig.5 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pCAG-loxP-stop-loxP-mir30 (plasmid)
Selectable markers Amp^r (E. coli).
Depositor|Developer Iwasato, Takuji |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。

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RDB15059 pK178.CAG-loxP-stop-loxP-mir30 (GFP RNAi scramble control) (Supernova) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pK178.CAG-loxP-stop-loxP-mir30 (GFP RNAi scramble control) (Supernova) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15059).

Reference section:

Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15059_A7Enp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB15059_A7Ena.seq check
>D04276B8_A7En_1_pAxCA_F1_G09_21_ABI24.ab1
    1 AATCATCTTC TACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG
   61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTGATTA ATTCGAGCGA ACGCGTACTA
  121 GTATAACTTC GTATAGCATA CATTATACGA GTTATTTTTT GAGCTCCATA ATCAGCCATA
  181 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
  241 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
  301 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
  361 GTGGTTTGTC CAAACTCATC AATGTATCCG CGGCATAATC AGCCATACCA CATTTGTAGA
  421 GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA
  481 TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  541 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  601 ACTCATCAAT GTATATCGAT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  661 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  721 CCAAACTCAT CAATGTATCT TATCATGTCT GGATCAAATC CGAATAACTT CGTATAGCAT
  781 ACATTATACG AAGTTATACT AGTCTCGATC GACTAGGGAT AACAGGGTAA TTGTTTGAAT
  841 GAGGCTTCAG TACTTTACAG AATCGTTGCC TGCACATCTT GGAAACACTT GCTGGGATTA
  901 CTTCTTCAGG TTAACCCAAC AGAAAGGCTC GAGAAAGGTA TATTGCTGTT GACAGTGAGC
  961 GAGCTCATAG AAGCTCTACA ATCTAGTGAA AGCCACAGAT GTAGATTTGT AGAGCTTCTA
 1021 TGAGCGTGCC TACTGCCTCG GGACTTCAAG GGGCTACTTT AAGAAGCCAT AATCTGATTA
1081 CTAGACTGAT ACTGGCCTAT CTCTTTGGAT ACATTTTTTT ACCCAAAC
//
Primer: pCA-bgter
Sequence file: RDB15059_A7Enb.seq check
>D04276B8_A7En_1_pCA-bgter_H09_24_ABI24.ab1
    1 AACCCAGGAG TCCATAATTT TTGGCAGAGG GAAAAAGATC TCAGTGGTAT TTGTGAGCCA
   61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGCGGC
  121 CGCATCGATG ATATCAAAAG TGATAATTTA TACCATTTTA ATTCAGCTTT GTAAAAATGT
  181 ATCAAAGAGA TAGCAAGGTA TTCAGTTTTA GTAAACAAGA TAATTGCTCC TAAAGTAGCC
  241 CCTTGAATTC CGAGGCAGTA GGCACGCTCA TAGAAGCTCT ACAATCTACA TCTGTGGCTT
  301 CACTAGATTG TAGAGCTTCT ATGAGCTCGC TCACTGTCAA CAGCAATATA CCTTCTCGAG
  361 CCTTCTGTTG GGTTAACCTG AAGAAGTAAT CCCAGCAAGT GTTTCCAAGA TGTGCAGGCA
  421 ACGATTCTGT AAAGTACTGA AGCCTCATTC AAACAATTAC CCTGTTATCC CTAGTCGATC
  481 GAGACTAGTA TAACTTCGTA TAATGTATGC TATACGAAGT TATTCGGATT TGATCCAGAC
  541 ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC
  601 TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA
  661 CAAGTTATCG ATATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA
  721 ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA
  781 TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG
  841 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGCCGCGGA
  901 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT
  961 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTTAA
 1021 CACCAACAAA TTGCATTTCA TTTTATGTTT CAGTTCACGG GGAAGTGTGG AGATTTTTAA
1081 AGGCAGTAGA CTCTACAATG TGTATGGCTG ATTTATGGAA GCCTCTAAAA AT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]