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pK177.CAG-loxP-stop-loxP-mir30 (GFP RNAi) (Supernova) (#RDB15058)

Cre-based Supernova series, a supernova-mediated RNAi enables sparsely labeled cell-specific gene knockdown without using genetically altered mice.

Alternative name pK177,
Supernova-mediated RNAi vectors
Clone info. Cre-based Supernova series, a supernova-mediated RNAi enables sparsely labeled cell-specific gene knockdown without using genetically altered mice. Cre-dependent GFP shRNA expression vector of loxP-stop-loxP-mir30 under the CAG promoter. Please refer Fig.5 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pCAG-loxP-stop-loxP-mir30 (plasmid)
Selectable markers Amp^r (E. coli).
Depositor|Developer Iwasato, Takuji |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15058 pK177.CAG-loxP-stop-loxP-mir30 (GFP RNAi) (Supernova) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pK177.CAG-loxP-stop-loxP-mir30 (GFP RNAi) (Supernova) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15058).

Reference section:

Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15058_A7Enp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164)
Region: SV40pAx3,loxP
Sequence file: RDB15058_A7Ena.seq check
>D04276B7_A7En_2_pAxCA_F1_G08_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TGCCTTCTTC TTTTTCCNAC NGCTCCTGGG CAACGTGCTG
   61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTGATTA ATTCGAGCGA ACGCGTACTA
  121 GTATAACTTC GTATAGCATA CATTATACGA GTTATTTTTT GAGCTCCATA ATCAGCCATA
  181 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
  241 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
  301 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
  361 GTGGTTTGTC CAAACTCATC AATGTATCCG CGGCATAATC AGCCATACCA CATTTGTAGA
  421 GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA
  481 TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  541 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  601 ACTCATCAAT GTATATCGAT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  661 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  721 CCAAACTCAT CAATGTATCT TATCATGTCT GGATCAAATC CGAATAACTT CGTATAGCAT
  781 ACATTATACG AAGTTATACT AGTCTCGATC GANTAGGGAT AACANGGTAA TTGNTTGAAT
  841 GANGCTTCAG TACTTTACAG AATCGTTGNC NGCACATCTT GNAAACACTN GCTGGGANTA
  901 CTTCTTCANG NTAANCNNAC AGAANGGNTC NANAANNNNA TATNGNTGNN NNNANNGANC
961 GAANCNNCNA NNNNCNANNN CNNNNNNNNN ANNNNNNNNN N
//
Primer: pCA-bgter (Pr0066)
Region: mir30(GFP RNAi), loxP, SV40 pAx2
Sequence file: RDB15058_A7Enb.seq check
>D04276B7_A7En_2_pCA-bgter_H08_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT TTTGNNNGAN GGANAAGNNC TCAGTGGTAT TTGTGAGCCA
   61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGCGGC
  121 CGCATCGATG ATATCAAAAG TGATAATTTA TACCATTTTA ATTCAGCTTT GTAAAAATGT
  181 ATCAAAGAGA TAGCAAGGTA TTCAGTTTTA GTAAACAAGA TAATTGCTCC TAAAGTAGCC
  241 CCTTGAATTC CGAGGCAGTA GGCACAGCCA CAACGTCTAT ATCATGTACA TCTGTGGCTT
  301 CACTACATGA TATAGACGTT GTGGCTTCGC TCACTGTCAA CAGCAATATA CCTTCTCGAG
  361 CCTTCTGTTG GGTTAACCTG AAGAAGTAAT CCCAGCAAGT GTTTCCAAGA TGTGCAGGCA
  421 ACGATTCTGT AAAGTACTGA AGCCTCATTC AAACAATTAC CCTGTTATCC CTAGTCGATC
  481 GAGACTAGTA TAACTTCGTA TAATGTATGC TATACGAAGT TATTCGGATT TGATCCAGAC
  541 ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC
  601 TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA
  661 CAAGTTATCG ATATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA
  721 ATGCTTTATT TGTGAAATTT GTGANGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA
  781 TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CANGTTCANG GGGAGGTGTG
  841 NGANGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGN ANGNNTGANT ANGCCNCGNA
  901 NACNTTNATG ANTTTGGNNA AACCNCAACT AGAATGCANN GAAAAAAAAT GNTTTATTNG
961 NGNNNTNNNN ANGCTNNTNN NNTNNNTNNN NNNNNNTNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]