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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK177.CAG-loxP-stop-loxP-mir30 (GFP RNAi) (Supernova)

Cre-based Supernova series, a supernova-mediated RNAi enables sparsely labeled cell-specific gene knockdown without using genetically altered mice.

Catalog number RDB15058
Resource name pK177.CAG-loxP-stop-loxP-mir30 (GFP RNAi) (Supernova)
Alternative name pK177,
Supernova-mediated RNAi vectors
Clone info. Cre-based Supernova series, a supernova-mediated RNAi enables sparsely labeled cell-specific gene knockdown without using genetically altered mice. Cre-dependent GFP shRNA expression vector of loxP-stop-loxP-mir30 under the CAG promoter. Please refer Fig.5 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pCAG-loxP-stop-loxP-mir30 (plasmid)
Selectable markers Amp^r (E. coli).
Depositor|Developer Iwasato, Takuji |
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            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。
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            RDB15058 pK177.CAG-loxP-stop-loxP-mir30 (GFP RNAi) (Supernova) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15058_A7Enp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Region: SV40pAx3,loxP
            Sequence file: RDB15058_A7Ena.seq check
            >D04276B7_A7En_2_pAxCA_F1_G08_20_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN TGCCTTCTTC TTTTTCCNAC NGCTCCTGGG CAACGTGCTG
               61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTGATTA ATTCGAGCGA ACGCGTACTA
              121 GTATAACTTC GTATAGCATA CATTATACGA GTTATTTTTT GAGCTCCATA ATCAGCCATA
              181 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
              241 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
              301 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
              361 GTGGTTTGTC CAAACTCATC AATGTATCCG CGGCATAATC AGCCATACCA CATTTGTAGA
              421 GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA
              481 TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
              541 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
              601 ACTCATCAAT GTATATCGAT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
              661 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
              721 CCAAACTCAT CAATGTATCT TATCATGTCT GGATCAAATC CGAATAACTT CGTATAGCAT
              781 ACATTATACG AAGTTATACT AGTCTCGATC GANTAGGGAT AACANGGTAA TTGNTTGAAT
              841 GANGCTTCAG TACTTTACAG AATCGTTGNC NGCACATCTT GNAAACACTN GCTGGGANTA
              901 CTTCTTCANG NTAANCNNAC AGAANGGNTC NANAANNNNA TATNGNTGNN NNNANNGANC
            961 GAANCNNCNA NNNNCNANNN CNNNNNNNNN ANNNNNNNNN N
            //
            Primer: pCA-bgter
            Region: mir30(GFP RNAi), loxP, SV40 pAx2
            Sequence file: RDB15058_A7Enb.seq check
            >D04276B7_A7En_2_pCA-bgter_H08_23_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNT TTTGNNNGAN GGANAAGNNC TCAGTGGTAT TTGTGAGCCA
               61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGCGGC
              121 CGCATCGATG ATATCAAAAG TGATAATTTA TACCATTTTA ATTCAGCTTT GTAAAAATGT
              181 ATCAAAGAGA TAGCAAGGTA TTCAGTTTTA GTAAACAAGA TAATTGCTCC TAAAGTAGCC
              241 CCTTGAATTC CGAGGCAGTA GGCACAGCCA CAACGTCTAT ATCATGTACA TCTGTGGCTT
              301 CACTACATGA TATAGACGTT GTGGCTTCGC TCACTGTCAA CAGCAATATA CCTTCTCGAG
              361 CCTTCTGTTG GGTTAACCTG AAGAAGTAAT CCCAGCAAGT GTTTCCAAGA TGTGCAGGCA
              421 ACGATTCTGT AAAGTACTGA AGCCTCATTC AAACAATTAC CCTGTTATCC CTAGTCGATC
              481 GAGACTAGTA TAACTTCGTA TAATGTATGC TATACGAAGT TATTCGGATT TGATCCAGAC
              541 ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC
              601 TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA
              661 CAAGTTATCG ATATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA
              721 ATGCTTTATT TGTGAAATTT GTGANGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA
              781 TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CANGTTCANG GGGAGGTGTG
              841 NGANGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGN ANGNNTGANT ANGCCNCGNA
              901 NACNTTNATG ANTTTGGNNA AACCNCAACT AGAATGCANN GAAAAAAAAT GNTTTATTNG
            961 NGNNNTNNNN ANGCTNNTNN NNTNNNTNNN NNNNNNTNNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl