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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK238.U6-gRNACreb1-CAG-loxP-stop-loxP-hspCas9 (Supernova) (#RDB15057)

Supernova series, a versatile vector system for single-cell labeling in vivo. Expression vector of both loxP-stop-loxP-Cas9 and gRNA (Creb1 as the target gene) under the CAG promoter.

Alternative name pK238,
vector for supernova CRISPR/CAS9
Clone info. Supernova series, a versatile vector system for single-cell labeling in vivo. Expression vector of both loxP-stop-loxP-Cas9 and gRNA (Creb1 as the target gene ) under the CAG promoter. This clone is used as Supernova-mediated CRISPR/Cas9 vectors. Please refer Fig.7 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pK237 (plasmid)
Selectable markers Amp^r (E. coli).
Depositor|Developer Iwasato, Takuji |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
提供案内 (日本国内) [open/close]

MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15057 pK238.U6-gRNACreb1-CAG-loxP-stop-loxP-hspCas9 (Supernova) DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pK238.U6-gRNACreb1-CAG-loxP-stop-loxP-hspCas9 (Supernova) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15057).

Reference section:

Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15057_A7Enp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse
Sequence file: RDB15057_A7Ena.seq check
>D04276B6_A7En_1_SV40pA3'element Reverse_A11_02_ABI24.ab1
    1 GTCGTCAGGG GGCGGAGCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTGAGG GCCTATTTCC CATGATTCCT TCATATTTGC
  121 ATATACGATA CAAGGCTGTT AGAGAGATAA TTGGAATTAA TTTGACTGTA AACACAAAGA
  181 TATTAGTACA AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA
  241 AATTATGTTT TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT
  301 TGGCTTTATA TATCTTGTGG AAAGGACGAA ACACCGTTGT CTGCTCCAGA TTCCAGTTTT
  361 AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC
  421 CGAGTCGGTG CTTTTTTGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT
  481 TTTTAGCGCG TGCGCCAATT CTGCAGACAA ATGGCTCTAG AGGTACCCGT TACATAACTT
  541 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAGTA
  601 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC
  661 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT
  721 AAATGGCCCG CCTGGCATTG TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG
  781 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG TGGAGCCCCA CGTTCTGCCT
  841 CACTCTCCCA TCTCCCCCCT CCCACCCATT TGATAATTAT TTTATATTTG GCACGATGGG
  901 TGCGGGGGGG CCCCCCAAGC GACCGCCGGC CAGGCCGGCT GCAGCATGCG CGACATCACG
961 GCGCTCAAGT CATAAGCGAG
//
Primer: pAxCA_F1
Sequence file: RDB15057_A7Enb.seq check
>D04276B6_A7En_1_pAxCA_F1_D11_11_ABI24.ab1
    1 GTTAACGTCC TGCTACATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCT AATAACTTCG TATAGCATAC
  121 ATTATACGAA GTTATATTAA GGGTTCCGGA TCCTCGGGGA CACCAAATAT GGCGATCTCG
  181 GCCTTTTCGT TTCTTGGAGC TGGGACATGT TTGCCATCGA TCCATCTACC ACCAGAACGG
  241 CCGTTAGATC TGCTGCCACC GTTGTTTCCA CCGAAGAAAC CACCGTTGCC GTAACCACCA
  301 CGACGGTTGT TGCTAAAGAA GCTGCCACCG CCACGGCCAC CGTTGTAGCC GCCGTTGTTG
  361 TTATTGTAGT TGCTACTGTT ATTTCTGGCA CTTCTTGGTT TTCCTCTTAA GTGAGGAGGA
  421 ACATAACCAT TCTCGTTGTT GTCGTTGATG CTTAAATTTT GCACTTGTTC GCTCAGTTCA
  481 GCCATAATAT GAAATGCTTT TCTTGTTGTT CTTACGGAAT ACCACTTGCC ACCTATCACC
  541 ACAACTAACT TTTTCCCGTT CCTCCATCTC TTTTATATTT TTTTTCTCGA GGGATCTTTG
  601 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA GAGATTTAAA
  661 GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT TAAACTACTG ATTCTAATTG
  721 TTTGTGTATT TTAGATTCCA ACCTATGGAA CTGATGAATG GGAGCAGTGG TGGAATGCCT
  781 TTAATGAGGA AAACCTGTTT TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG
  841 CTGACTCTCA ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT
  901 TTCTTCAGAA TTGCTAGTTT TTTGAGTCAT GCTGTGTTTA GTAATAGAAC TCTGCTGCTT
  961 TGCTATTTAC ACCCACAAAG AAAAGCCTGC ACTGCCTATA CAAGGAAAAG TATGGAAAAT
 1021 ATTCCTGTAC TTTAAGTTAG GCTTAACAGT TATATCTACA TCTGTTTCTA TCCACAGCAT
1081 AAAAGTTC
//
Primer: pAxCALNLF1
Sequence file: RDB15057_A7Enc.seq check
>D04276B6_A7En_1_pAxCALNLF1_B11_05_ABI24.ab1
    1 TTTTATGGTA TCTTATCATG TCTGGATCTG ACATGGTAAG TAAGCTTGGG CTGCAGGTCG
   61 AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATATGTCGA CGGTACCGCG
  121 GGCCCGGGAT CCACCGGTGC CACCATGGAC TATAAGGACC ACGACGGAGA CTACAAGGAT
  181 CATGATATTG ATTACAAAGA CGATGACGAT AAGATGGCCC CAAAGAAGAA GCGGAAGGTC
  241 GGTATCCACG GAGTCCCAGC AGCCGACAAG AAGTACAGCA TCGGCCTGGA CATCGGCACC
  301 AACTCTGTGG GCTGGGCCGT GATCACCGAC GAGTACAAGG TGCCCAGCAA GAAATTCAAG
  361 GTGCTGGGCA ACACCGACCG GCACAGCATC AAGAAGAACC TGATCGGAGC CCTGCTGTTC
  421 GACAGCGGCG AAACAGCCGA GGCCACCCGG CTGAAGAGAA CCGCCAGAAG AAGATACACC
  481 AGACGGAAGA ACCGGATCTG CTATCTGCAA GAGATCTTCA GCAACGAGAT GGCCAAGGTG
  541 GACGACAGCT TCTTCCACAG ACTGGAAGAG TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC
  601 GAGCGGCACC CCATCTTCGG CAACATCGTG GACGAGGTGG CCTACCACGA GAAGTACCCC
  661 ACCATCTACC ACCTGAGAAA GAAACTGGTG GACAGCACCG ACAAGGCCGA CCTGCGGCTG
  721 ATCTATCTGG CCCTGGCCCA CATGATCAAG TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC
  781 CTGAACCCCG ACAACAGCGA CGTGGACAAG CTGTTCATCC AGCTGGTGCA GACCTACAAC
  841 CAGCTGTTCG AGGAAAACCC CATCAACGCC AGCGGCGTGG ACGCCAAGGC CATCCTGTCT
  901 GCCAGACTGA GCAAGAGCAG ACGGCTGGAA AATCTGATCG CCCAGCTGCC CGGCGAGAAG
  961 AAGATGGTCC TGTTCGGAAC CTGATTGCCC TGAGCCTGGG CTGACCCCAC TTTCAGAGCC
 1021 ACTCGAACTG CGAGATGCCT AACTGCAGCT GAACAAGACA CTACGACGAC GACCTTGGAC
1081 ACCTGGCCTT GGACCAGATT CGGGGCGAC
//
Primer: BGH_rev2
Sequence file: RDB15057_A7End.seq check
>D04276B6_A7En_1_BGH_rev2_C11_08_ABI24.ab1
    1 TATCACGTCA AGACGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGAG CTCTAGGAAT TCTTACTTTT TCTTTTTTGC CTGGCCGGCC
  121 TTTTTCGTGG CCGCCGGCCT TTTGTCGCCT CCCAGCTGAG ACAGGTCGAT CCGTGTCTCG
  181 TACAGGCCGG TGATGCTCTG GTGGATCAGG GTGGCGTCCA GCACCTCTTT GGTGCTGGTG
  241 TACCTCTTCC GGTCGATGGT GGTGTCAAAG TACTTGAAGG CGGCAGGGGC TCCCAGATTG
  301 GTCAGGGTAA ACAGGTGGAT GATATTCTCG GCCTGCTCTC TGATGGGCTT ATCCCGGTGC
  361 TTGTTGTAGG CGGACAGCAC TTTGTCCAGA TTAGCGTCGG CCAGGATCAC TCTCTTGGAG
  421 AACTCGCTGA TCTGCTCGAT GATCTCGTCC AGGTAGTGCT TGTGCTGTTC CACAAACAGC
  481 TGTTTCTGCT CATTATCCTC GGGGGAGCCC TTCAGCTTCT CATAGTGGCT GGCCAGGTAC
  541 AGGAAGTTCA CATATTTGGA GGGCAGGGCC AGTTCGTTTC CCTTCTGCAG TTCGCCGGCA
  601 GAGGCCAGCA TTCTCTTCCG GCCGTTTTCC AGCTCGAACA GGGAGTACTT AGGCAGCTTG
  661 ATGATCAGGT CCTTTTTCAC TTCTTTGTAG CCCTTGGCTT CCAGAAAGTC GATGGGATTC
  721 TTCTCGAAGC TGCTTCTTTC CATGATGGTG ATCCCCAGCA GCTCTTTCAC ACTCTTCAGT
  781 TTCTTGGACT TGCCCTTTTC CACTTTGGCC ACCACCAGCA CAGAATAGGC CACGGTGGGG
  841 GCTGTCGAAG CCGCCGTACT TCTTAGGGTC CCAGTCCTTC TTTCTGGCGA TCAGCTTATC
  901 GCTGTTCCTC TTGGGCAGGA TAGACTCTTT GCTGAAGCCG CCTGTCTGCA CCTCGGTCTT
  961 TTTCACGATA TTCACTTGGG GCATGCTCAG CACTTTCCGC ACGGGTGGGC AAAATCCCGG
 1021 CCCCTTATCC CACACGAATC TCCCCGGTTT TCGCGTTTGT TCTCGAATCA GAGCGCTTCT
1081 GGATCTCGGC CGATGGCCAG GTATCTCGGT CTGGAAAAGT TCATTGAATT GTTTGGC
//
Primer: f1ori_R3
Sequence file: RDB15057e_A7En.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]