Please review the QC test results indicated by icon below as well as clone information before placing your order.

pK238.U6-gRNACreb1-CAG-loxP-stop-loxP-hspCas9 (Supernova)

Supernova series, a versatile vector system for single-cell labeling in vivo. Expression vector of both loxP-stop-loxP-Cas9 and gRNA (Creb1 as the target gene) under the CAG promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15057_A7Enp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse Sequence file: RDB15057_A7Ena.seq
>D04276B6_A7En_1_SV40pA3'element Reverse_A11_02_ABI24.ab1     1 GTCGTCAGGG GGCGGAGCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC    61 CTTTTGCTGG CCTTTTGCTC ACATGTGAGG GCCTATTTCC CATGATTCCT TCATATTTGC   121 ATATACGATA CAAGGCTGTT AGAGAGATAA TTGGAATTAA TTTGACTGTA AACACAAAGA   181 TATTAGTACA AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA   241 AATTATGTTT TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT   301 TGGCTTTATA TATCTTGTGG AAAGGACGAA ACACCGTTGT CTGCTCCAGA TTCCAGTTTT   361 AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC   421 CGAGTCGGTG CTTTTTTGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT   481 TTTTAGCGCG TGCGCCAATT CTGCAGACAA ATGGCTCTAG AGGTACCCGT TACATAACTT   541 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAGTA   601 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC   661 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT   721 AAATGGCCCG CCTGGCATTG TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG   781 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG TGGAGCCCCA CGTTCTGCCT   841 CACTCTCCCA TCTCCCCCCT CCCACCCATT TGATAATTAT TTTATATTTG GCACGATGGG   901 TGCGGGGGGG CCCCCCAAGC GACCGCCGGC CAGGCCGGCT GCAGCATGCG CGACATCACG 961 GCGCTCAAGT CATAAGCGAG //
Primer: pAxCA_F1 Sequence file: RDB15057_A7Enb.seq
>D04276B6_A7En_1_pAxCA_F1_D11_11_ABI24.ab1     1 GTTAACGTCC TGCTACATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG    61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCT AATAACTTCG TATAGCATAC   121 ATTATACGAA GTTATATTAA GGGTTCCGGA TCCTCGGGGA CACCAAATAT GGCGATCTCG   181 GCCTTTTCGT TTCTTGGAGC TGGGACATGT TTGCCATCGA TCCATCTACC ACCAGAACGG   241 CCGTTAGATC TGCTGCCACC GTTGTTTCCA CCGAAGAAAC CACCGTTGCC GTAACCACCA   301 CGACGGTTGT TGCTAAAGAA GCTGCCACCG CCACGGCCAC CGTTGTAGCC GCCGTTGTTG   361 TTATTGTAGT TGCTACTGTT ATTTCTGGCA CTTCTTGGTT TTCCTCTTAA GTGAGGAGGA   421 ACATAACCAT TCTCGTTGTT GTCGTTGATG CTTAAATTTT GCACTTGTTC GCTCAGTTCA   481 GCCATAATAT GAAATGCTTT TCTTGTTGTT CTTACGGAAT ACCACTTGCC ACCTATCACC   541 ACAACTAACT TTTTCCCGTT CCTCCATCTC TTTTATATTT TTTTTCTCGA GGGATCTTTG   601 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA GAGATTTAAA   661 GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT TAAACTACTG ATTCTAATTG   721 TTTGTGTATT TTAGATTCCA ACCTATGGAA CTGATGAATG GGAGCAGTGG TGGAATGCCT   781 TTAATGAGGA AAACCTGTTT TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG   841 CTGACTCTCA ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT   901 TTCTTCAGAA TTGCTAGTTT TTTGAGTCAT GCTGTGTTTA GTAATAGAAC TCTGCTGCTT   961 TGCTATTTAC ACCCACAAAG AAAAGCCTGC ACTGCCTATA CAAGGAAAAG TATGGAAAAT  1021 ATTCCTGTAC TTTAAGTTAG GCTTAACAGT TATATCTACA TCTGTTTCTA TCCACAGCAT 1081 AAAAGTTC //
Primer: pAxCALNLF1 Sequence file: RDB15057_A7Enc.seq
>D04276B6_A7En_1_pAxCALNLF1_B11_05_ABI24.ab1     1 TTTTATGGTA TCTTATCATG TCTGGATCTG ACATGGTAAG TAAGCTTGGG CTGCAGGTCG    61 AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATATGTCGA CGGTACCGCG   121 GGCCCGGGAT CCACCGGTGC CACCATGGAC TATAAGGACC ACGACGGAGA CTACAAGGAT   181 CATGATATTG ATTACAAAGA CGATGACGAT AAGATGGCCC CAAAGAAGAA GCGGAAGGTC   241 GGTATCCACG GAGTCCCAGC AGCCGACAAG AAGTACAGCA TCGGCCTGGA CATCGGCACC   301 AACTCTGTGG GCTGGGCCGT GATCACCGAC GAGTACAAGG TGCCCAGCAA GAAATTCAAG   361 GTGCTGGGCA ACACCGACCG GCACAGCATC AAGAAGAACC TGATCGGAGC CCTGCTGTTC   421 GACAGCGGCG AAACAGCCGA GGCCACCCGG CTGAAGAGAA CCGCCAGAAG AAGATACACC   481 AGACGGAAGA ACCGGATCTG CTATCTGCAA GAGATCTTCA GCAACGAGAT GGCCAAGGTG   541 GACGACAGCT TCTTCCACAG ACTGGAAGAG TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC   601 GAGCGGCACC CCATCTTCGG CAACATCGTG GACGAGGTGG CCTACCACGA GAAGTACCCC   661 ACCATCTACC ACCTGAGAAA GAAACTGGTG GACAGCACCG ACAAGGCCGA CCTGCGGCTG   721 ATCTATCTGG CCCTGGCCCA CATGATCAAG TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC   781 CTGAACCCCG ACAACAGCGA CGTGGACAAG CTGTTCATCC AGCTGGTGCA GACCTACAAC   841 CAGCTGTTCG AGGAAAACCC CATCAACGCC AGCGGCGTGG ACGCCAAGGC CATCCTGTCT   901 GCCAGACTGA GCAAGAGCAG ACGGCTGGAA AATCTGATCG CCCAGCTGCC CGGCGAGAAG   961 AAGATGGTCC TGTTCGGAAC CTGATTGCCC TGAGCCTGGG CTGACCCCAC TTTCAGAGCC  1021 ACTCGAACTG CGAGATGCCT AACTGCAGCT GAACAAGACA CTACGACGAC GACCTTGGAC 1081 ACCTGGCCTT GGACCAGATT CGGGGCGAC //
Primer: BGH_rev2 Sequence file: RDB15057_A7End.seq
>D04276B6_A7En_1_BGH_rev2_C11_08_ABI24.ab1     1 TATCACGTCA AGACGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GATCAGCGAG CTCTAGGAAT TCTTACTTTT TCTTTTTTGC CTGGCCGGCC   121 TTTTTCGTGG CCGCCGGCCT TTTGTCGCCT CCCAGCTGAG ACAGGTCGAT CCGTGTCTCG   181 TACAGGCCGG TGATGCTCTG GTGGATCAGG GTGGCGTCCA GCACCTCTTT GGTGCTGGTG   241 TACCTCTTCC GGTCGATGGT GGTGTCAAAG TACTTGAAGG CGGCAGGGGC TCCCAGATTG   301 GTCAGGGTAA ACAGGTGGAT GATATTCTCG GCCTGCTCTC TGATGGGCTT ATCCCGGTGC   361 TTGTTGTAGG CGGACAGCAC TTTGTCCAGA TTAGCGTCGG CCAGGATCAC TCTCTTGGAG   421 AACTCGCTGA TCTGCTCGAT GATCTCGTCC AGGTAGTGCT TGTGCTGTTC CACAAACAGC   481 TGTTTCTGCT CATTATCCTC GGGGGAGCCC TTCAGCTTCT CATAGTGGCT GGCCAGGTAC   541 AGGAAGTTCA CATATTTGGA GGGCAGGGCC AGTTCGTTTC CCTTCTGCAG TTCGCCGGCA   601 GAGGCCAGCA TTCTCTTCCG GCCGTTTTCC AGCTCGAACA GGGAGTACTT AGGCAGCTTG   661 ATGATCAGGT CCTTTTTCAC TTCTTTGTAG CCCTTGGCTT CCAGAAAGTC GATGGGATTC   721 TTCTCGAAGC TGCTTCTTTC CATGATGGTG ATCCCCAGCA GCTCTTTCAC ACTCTTCAGT   781 TTCTTGGACT TGCCCTTTTC CACTTTGGCC ACCACCAGCA CAGAATAGGC CACGGTGGGG   841 GCTGTCGAAG CCGCCGTACT TCTTAGGGTC CCAGTCCTTC TTTCTGGCGA TCAGCTTATC   901 GCTGTTCCTC TTGGGCAGGA TAGACTCTTT GCTGAAGCCG CCTGTCTGCA CCTCGGTCTT   961 TTTCACGATA TTCACTTGGG GCATGCTCAG CACTTTCCGC ACGGGTGGGC AAAATCCCGG  1021 CCCCTTATCC CACACGAATC TCCCCGGTTT TCGCGTTTGT TCTCGAATCA GAGCGCTTCT 1081 GGATCTCGGC CGATGGCCAG GTATCTCGGT CTGGAAAAGT TCATTGAATT GTTTGGC //
Primer: f1ori_R3 Sequence file: RDB15057e_A7En.seq
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl