Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova)

Cre-based Supernova series. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream AmCyan is expressed.

Catalog number RDB15056
Resource name pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova)
Alternative name pK039,
Cre based supernova XFP
Clone info. Cre-based Supernova series. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream AmCyan is expressed. Expression vector of loxP-stop-loxP-AmCyan under the CAG promoter. Please refer Supplemetary Figs. 1 and 2a of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pK029 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Depositor|Developer Iwasato, Takuji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Exclusive MTA (for use for not-for-profit academic purpose)[Link]
Remarks
The BIOLOGICAL RESOURCE may not be used for COMMERCIAL PURPOSES. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs).
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15056 pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 20775045.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15056_A7Enp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB15056_A7Ena.seq check
//
Primer: pAxCALNLF1
Sequence file: RDB15056_A7Enb.seq check
//
Primer: PRE_rev
Sequence file: RDB15056_A7Enc.seq check
>D04276B5_A7En_1_PRE_rev_E10_13_ABI24.ab1
    1 TCCCATTCCA CATAAGCCGT AAAAAGGAGC ACATAGTTAA GAATACCAGT CAATCTTTCA
   61 CAAATTTTGT AATCCAGAGG TTGAGCGGCC GAAGGGCGAA TTCGCGGCCG CTTACTTAGT
  121 TACCCGGGGA GCATGTCAAG GTCAAAATCG TCAAGAGCGT CAGCAGGCAG CATATCAAGG
  181 TCAAAGTCGT CAAGGGCATC GGCTGGGAGC ATGTCTAAGT CAAAATCGTC AAGGGCGTCG
  241 GCCGGCCCGC CGCTTTCGCA CTTTAGCTGT TTCTCCAGGC CACATATGAT TAGTTCCAGG
  301 CCGAAAAGGA AGGCAGGTTC GGCTCCCTGA TGGTCGAACA GCTCAATTGC TTGTCTCAGA
  361 AGTGGGGGCA TAGAATCGGT GGTAGGTGTC TCTCTTTCCT CTTTTGCTAC TTGATGCTCC
  421 TGATCCTCCA ATACGCAGCC CAGTGTAAAG TGGCCCACGG CGGACAGAGC GTACAGTGCG
  481 TTCTCCAGGG AGAAGCCTTG CTGACACAGG AACGCGAGCT GATTTTCCAG GGTTTCGTAC
  541 TGTTTCTCTG TTGGGCGGGT GCCGAGATGC ACTTTAGCCC CGTCGCGATG TGAGAGGAGA
  601 GCACAGCGGA ATGACTTGGC GTTGTTCCGC AGAAAGTCTT GCCATGACTC GCCTTCCAGG
  661 GGGCAGAAGT GGGTATGATG CCTGTCCAGC ATCTCGATTG CCAGGGCATC GAGCAGGGCC
  721 CGCTTGTTCT TCACGTGCCA GTACAGGGTA GGCTGCTCAA CTCCCAGCTT TTGAGCGAGT
  781 TTCCTTGTCG TCAGGCCTTC GATACCGACT TCATTGAGTA ATTCCAGAGC AGAGTTTATG
  841 ACTTTGCTCT TGTCCAGTCT AGACATCGTG TTTTTCAAAG GAAACCACGT CCCCGTGGGT
  901 TCGGGGGGGC CTAGACGTTT TTTTTAACCT CGACTAAAAC ACATGTAAAG CATGTGCACC
  961 GGAGGCCCCC AGATCAGATC CCATACAATG GGGGTACCCT TCTGGGGCAT CCCTTTCAGC
 1021 CCCTTGTTTG AAATACGGCT TGAGGAGAGC CCATTTTTAC TCTTTCCACA AACTATCCAA
 1081 CTTCACAACG GTGGGCACTG GGATTGATGC CCGGCCCTTT TGGGCCAGGG GTGTAATCTT
1141 AAATACAACA CGATTG
//
Primer: SV40pro_F_V2
Sequence file: RDB15056_A7End.seq check
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 20775045.

2020.12.14

GNP_filter3_RDBDEP_html_201104.pl