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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova)

Cre-based Supernova series. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream AmCyan is expressed.

Catalog number RDB15056
Resource name pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova)
Alternative name pK039,
Cre based supernova XFP
Clone info. Cre-based Supernova series. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream AmCyan is expressed. Expression vector of loxP-stop-loxP-AmCyan under the CAG promoter. Please refer Supplemetary Figs. 1 and 2a of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pK029 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Depositor|Developer Iwasato, Takuji |
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            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。
            付加的提供条件：
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
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            RDB15056 pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15056).

            Reference section:

            Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            Featured content Expression regulation (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15056_A7Enp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Sequence file: RDB15056_A7Ena.seq check
            1
            Primer: pAxCALNLF1
            Sequence file: RDB15056_A7Enb.seq check
            1
            Primer: PRE_rev
            Sequence file: RDB15056_A7Enc.seq check
            >D04276B5_A7En_1_PRE_rev_E10_13_ABI24.ab1
                1 TCCCATTCCA CATAAGCCGT AAAAAGGAGC ACATAGTTAA GAATACCAGT CAATCTTTCA
               61 CAAATTTTGT AATCCAGAGG TTGAGCGGCC GAAGGGCGAA TTCGCGGCCG CTTACTTAGT
              121 TACCCGGGGA GCATGTCAAG GTCAAAATCG TCAAGAGCGT CAGCAGGCAG CATATCAAGG
              181 TCAAAGTCGT CAAGGGCATC GGCTGGGAGC ATGTCTAAGT CAAAATCGTC AAGGGCGTCG
              241 GCCGGCCCGC CGCTTTCGCA CTTTAGCTGT TTCTCCAGGC CACATATGAT TAGTTCCAGG
              301 CCGAAAAGGA AGGCAGGTTC GGCTCCCTGA TGGTCGAACA GCTCAATTGC TTGTCTCAGA
              361 AGTGGGGGCA TAGAATCGGT GGTAGGTGTC TCTCTTTCCT CTTTTGCTAC TTGATGCTCC
              421 TGATCCTCCA ATACGCAGCC CAGTGTAAAG TGGCCCACGG CGGACAGAGC GTACAGTGCG
              481 TTCTCCAGGG AGAAGCCTTG CTGACACAGG AACGCGAGCT GATTTTCCAG GGTTTCGTAC
              541 TGTTTCTCTG TTGGGCGGGT GCCGAGATGC ACTTTAGCCC CGTCGCGATG TGAGAGGAGA
              601 GCACAGCGGA ATGACTTGGC GTTGTTCCGC AGAAAGTCTT GCCATGACTC GCCTTCCAGG
              661 GGGCAGAAGT GGGTATGATG CCTGTCCAGC ATCTCGATTG CCAGGGCATC GAGCAGGGCC
              721 CGCTTGTTCT TCACGTGCCA GTACAGGGTA GGCTGCTCAA CTCCCAGCTT TTGAGCGAGT
              781 TTCCTTGTCG TCAGGCCTTC GATACCGACT TCATTGAGTA ATTCCAGAGC AGAGTTTATG
              841 ACTTTGCTCT TGTCCAGTCT AGACATCGTG TTTTTCAAAG GAAACCACGT CCCCGTGGGT
              901 TCGGGGGGGC CTAGACGTTT TTTTTAACCT CGACTAAAAC ACATGTAAAG CATGTGCACC
              961 GGAGGCCCCC AGATCAGATC CCATACAATG GGGGTACCCT TCTGGGGCAT CCCTTTCAGC
             1021 CCCTTGTTTG AAATACGGCT TGAGGAGAGC CCATTTTTAC TCTTTCCACA AACTATCCAA
             1081 CTTCACAACG GTGGGCACTG GGATTGATGC CCGGCCCTTT TGGGCCAGGG GTGTAATCTT
            1141 AAATACAACA CGATTG
            //
            Primer: SV40pro_F_V2
            Sequence file: RDB15056_A7End.seq check
            1

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]

            2023.12.12

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